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Author Profile
Brahma Kumar
Graduate Student
Columbia University
Articles by Brahma Kumar
Antibody User Review
Decent CD8 Antibody
September 30, 2016
This is a decent CD8 antibody. Easy to use and works well at 1:50. Not as bright as I would expect from a BV650 color. Signal is ...
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Antibody User Review
Good Inexpensive CD8 Antibody
September 29, 2016
Antibody works great and isn't expensive. Use at 1:50. Some issues compensating this color with APC but not a huge problem.
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Antibody User Review
Great CD3 Antibody
September 28, 2016
I use this antibody for identification of T cells. This antibody can be used for a variety of experiments, for example identifying...
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Antibody User Review
CD4 Antibody That Works, Nothing Special Though
September 27, 2016
This antibody gives clear separation but is not as bright as I would expect from a BV711 color. Easy to use, works well at 1:50
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Antibody User Review
One Of The Few Human CCR7 Antibodies That Works Well
September 26, 2016
I have tried many CCR7 antibodies and this is one of the few that works. Separation is ok, but when plotted against CD45RA easy to...
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Antibody User Review
Decent CD19 Antibody
September 23, 2016
Separation is reasonable, not excellent, but i use this for excluding B cells from my analyses of T cells. I use it at 1:100.
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Antibody User Review
Good CD45RA Antibody From Biolegend
September 22, 2016
This is a reliably good anti CD45RA antibody from biolegend. BV605 is quite bright and may not be necessary for such an abundant ...
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Antibody User Review
Excellent CD69 Antibody
September 21, 2016
I have used many CD69 antibodies and this is one of the best. For a marker like cd69 that exists at a variety of expression levels...
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Antibody User Review
Great CD4 Antibody From Biolegend
September 19, 2016
This antibody works very well and is extremely bright. I typically use at 1:100 or 1:200 but still get separation and even lower ...
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Antibody User Review
Very Good CD3 Antibody
September 01, 2016
This APC CD3 antibody from biolegend works quite well. Works best at 1:200 or even 1:400. Even at 1:800 get good separation. ...
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Antibody User Review
BUV805 CD8 Works Well
August 30, 2016
This antihuman BUV805 from BD works well. it is quite dim, which is good for abundant marker like CD8. Works optimally at 1:25 or ...
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Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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