Biolegend
Anti-human CD4 PeCy7
344612
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Browse Similar Products: CD4 Flow Cytometry Antibodies
This antibody works very well and is extremely bright. I typically use at 1:100 or 1:200 but still get separation and even lower concentrations. Separation is good after fix perm as well so suitable for ICS applications
Flow Cytometry
Human lymph node
30 min on ice
None
BD Fortessa
Image uploaded shows CD3 on x axis and CD4 on y axis. Very clear separation. I have used this antibody on a variety of human samples with good results.
Very clear separation
Pricey, perhaps too bright for an abundant marker like CD4
Great CD4 antibody