Ramanath Hegde

Postdoctoral fellow

Institute of Protein Biochemistry, Italian National Research Council, Napoli

Antibody User Review
LC3B Antibody
September 16, 2016
Upon induction of autophagy LC3B-I is converted to LC3B-II through lipidation. This conversion is used to indicate the induction ... read more
Antibody User Review
p-c-JUN antibody for Western Blotting
September 02, 2016
c-Jun phosphorylated at Serine 63 by JNK. So the levels of Phospho-c-Jun (Ser63) is also used as a measure of activation status of... read more
Antibody User Review
Myc-Tag Antibody to Detect the Expression of Myc -Tagged Proteins
September 01, 2016
Detection of proteins which have no available antibody are made easy by epitope tags. These tags allow the labeling and detection ... read more
Antibody User Review
Sar1 Antibody For Western Blotting
August 31, 2016
Sar1 is essential to the formation of transport vesicles from ER. There are two isoforms of Sar1 in mammalians Sar1A and Sar1B. ... read more
Antibody User Review
Anti CFTR Antibody For Fluorescence And Western Blotting
August 30, 2016
Untagged CFTR is expressed in HeLa cells and was stained using the Human CFTR C-Terminus Antibody (MAB25031). The antibody stains... read more
Antibody User Review
Active p38 alpha Blotting
August 29, 2016
Many signals via MKK3, MKK6, activate p38 alpha MAPK by phosphorylation at Thr180 and Tyr182, which in turn phosphorylates a many ... read more
Antibody User Review
TGN46 Fluorescence Staining
August 23, 2016
TGN46 is a trans-Golgi localized protein and it can be used to access any protein that may be localized to trans-Golgi by ... read more
Antibody User Review
CAMKK2 Antibody For Western Blotting
August 22, 2016
The CAMKK2 siRNAs were treated to cells for 72 hours and efficacy and effectiveness of siRNA in CAMKK2 protein knockdown needed to... read more
Antibody User Review
GRASP55 Antibody For Immuno Staining
August 19, 2016
GRASP55 is important for assembly and membrane stacking of the Golgi cisternae. It can be used as a Golgi marker. read more
Antibody User Review
Syntaxin 5 Antibody For Immunostaining
August 18, 2016
Syntaxin-5 is known to locaize to the Endoplasmic reticulum-Golgi intermediate compartment membrane and Golgi apparatus (abundant ... read more
Antibody User Review
Detection of MLK3 Protein Levels Using Westen Blotting
April 04, 2016
Microarray study determined that MLK3 levels were going down upon treatment of drug of our interest (DRUG1, DRUG2). We tested the ... read more
Antibody User Review
Detection of CFTR Using This Anti-CFTR Antibody
March 31, 2016
Cell lysates of CFBE cells expressing CFTR was resolved by SDS-PAGE followed by western blotting. There will be two distinct bands... read more
Assay Kit/Reagent User Review
Assay to Monitor Proteasome Dysfunction
March 18, 2016
We wanted to test the proteasome function. So we used Proteasome Sensor Vector ZsProSensor-1 (Clontech #632425). ZsProSensor-1 is... read more
Antibody User Review
Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb Antibody Review
March 08, 2016
We are interested in studying a protein that we hypothesized to activate JNK when it is expressed in the cell. We transfected this... read more

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Efficient and Cost Friendly SYBR Green Mastermix for qPCR

Parijat Sarkar

I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ... read more

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