EMD Millipore
Anti-Sar1 Antibody
07-692
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Browse Similar Products: Sar1 Western Blot Antibodies
Sar1 is essential to the formation of transport vesicles from ER. There are two isoforms of Sar1 in mammalians Sar1A and Sar1B. Anti-Sar1 Antibody 07-692 recognizes both the isoforms in the western blotting.
Western Blot
HeLa cell lysate -20ug
Primary antibody (1:2000) in dilution buffer (TBST with 5% BSA) with gentle agitation overnight at 4°C.
TBST with 5% w/v nonfat dry milk
Incubated the membrane with the Goat Anti-Rabbit IgG, H & L Chain Specific Peroxidase Conjugate (Cat No: 401315) 1:10000 diluted in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
None
Incubated the membrane with appropriate amount of Luminata Crescendo Western HRP substrate (WBLUR0100 Merck Millipore) for 5 min at room temperature. Drained the membrane of excess developing solution (without letting it to dry) wrapped in transparent plastic sheet and exposed the x-ray film for 1 min.
The anti Sar1 antibody recognizes Sa1A and Sar1B isoforms and this is evident when Sa1A and Sar1B siRNA treated the corresponding protein levels become very low compared ti scramble siRNA treatment
Not useful for immunofluorescence
Detects Sar1 specifically without background.
Antibody is very sensitive to detect Sar1 isoforms.