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Author Profile
Monica Valencia
RA3
University of Miami
Articles by Monica Valencia
Assay Kit/Reagent User Review
Homogenization and Genomic DNA Eliminator in 1 Column
October 20, 2015
This is a homogenizer and genomic DNA eliminator column 2 in 1. It's the first step to do after lysing your cells; no more time-...
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Assay Kit/Reagent User Review
Protease Inhibitor
October 19, 2015
This protease inhibitor cocktail is great for using to lyse cells to run western blots; it prevents protein degradation.
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Assay Kit/Reagent User Review
DNA Extraction
October 13, 2015
We constantly have to sequence plasmid DNA in our lab. Before going to a maxi prep we always do a mini prep to send it for ...
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Assay Kit/Reagent User Review
RNA Extraction Kit
October 02, 2015
I use this kit in the lab to extract RNA from cells. The RNA Integrity is quite high and it is an easy kit to use.
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Assay Kit/Reagent User Review
Cleansolutions Spray from Akron- Great to Clean Tissue Culture Room Equipment
May 22, 2015
Cleansolutions spray from Akron can be used to clean tissue culture hoods and incubators. It doesn't smell, it doesn't corrode. It...
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Antibody User Review
copGFP Western blot
April 30, 2015
In our lab we use copGFP as a marker for lentiviral infection, that way we can confirm either by immunofluorescence or Western ...
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Assay Kit/Reagent User Review
Tryple- Alternative to trypsin
April 24, 2015
This product can be used for very fast dissociation of hard to trypsinize cells without the need to inactivate with FBS or SBTI. ...
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Antibody User Review
Immunofluorescence staining using Life Technologies AF 2nd Ab
April 20, 2015
We do a lot of confocal microscopy in our laboratory. Life technologies is one of our favorite companies to get 2nd antibodies ...
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Equipment User Review
Smoke Robot
April 16, 2015
We study the effects of cigarette smoke in airway epithelial cells. This is a great model to expose the cells to whole cigarette ...
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Antibody User Review
EPAC1 Western blot
April 14, 2015
Western blot of extracts of whole mouse brain was done using EPAC1 antibody. We wanted to see whether the protein was expressed in...
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Antibody User Review
MUC5AC Immunfluorescence Staining in NHBE
April 13, 2015
This antibody was purchased to use as a marker of goblet cells in airway epithelial cells.
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Antibody User Review
E-cadherin / CDH1 Antibody Immunofluorescence
April 10, 2015
Cadherin formation is one of the steps among many in the process of airway epithelial cell differentiation. In our laboratory we ...
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Antibody User Review
ZO-1/TJP1 Immunofluorescence
April 09, 2015
In our laboratory we have to track airway epithelial cells throughout differentiation. Tight junction formation is among those ...
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Antibody User Review
Secondary Ab donkey anti Rabbit AF555
April 08, 2015
Secondary antibodies from Thermo Fisher Scientific are great for doing immunofluorescence. We work with primary cells (airway ...
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Antibody User Review
Donkey Anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 Conjugate
March 05, 2015
This is a great product to do immunofluorescence. We study primary cells (airway epithelial cells) in our lab and can use several ...
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Product Review
Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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