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Author Profile
Monica Valencia
RA3
University of Miami
Articles by Monica Valencia
Homogenization and Genomic DNA Eliminator in 1 Column
October 20, 2015
This is a homogenizer and genomic DNA eliminator column 2 in 1. It's the first step to do after lysing your cells; no more time-...
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Protease Inhibitor
October 19, 2015
This protease inhibitor cocktail is great for using to lyse cells to run western blots; it prevents protein degradation.
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DNA Extraction
October 13, 2015
We constantly have to sequence plasmid DNA in our lab. Before going to a maxi prep we always do a mini prep to send it for ...
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RNA Extraction Kit
October 02, 2015
I use this kit in the lab to extract RNA from cells. The RNA Integrity is quite high and it is an easy kit to use.
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Cleansolutions Spray from Akron- Great to Clean Tissue Culture Room Equipment
May 22, 2015
Cleansolutions spray from Akron can be used to clean tissue culture hoods and incubators. It doesn't smell, it doesn't corrode. It...
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copGFP Western blot
April 30, 2015
In our lab we use copGFP as a marker for lentiviral infection, that way we can confirm either by immunofluorescence or Western ...
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Tryple- Alternative to trypsin
April 24, 2015
This product can be used for very fast dissociation of hard to trypsinize cells without the need to inactivate with FBS or SBTI. ...
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Purchasing through Quartzy
April 21, 2015
Purchasing through Quartzy can potentially save lots of money. It gives you the advantage of potentially finding better prices ...
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Immunofluorescence staining using Life Technologies AF 2nd Ab
April 20, 2015
We do a lot of confocal microscopy in our laboratory. Life technologies is one of our favorite companies to get 2nd antibodies ...
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Smoke Robot
April 16, 2015
We study the effects of cigarette smoke in airway epithelial cells. This is a great model to expose the cells to whole cigarette ...
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EPAC1 Western blot
April 14, 2015
Western blot of extracts of whole mouse brain was done using EPAC1 antibody. We wanted to see whether the protein was expressed in...
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MUC5AC Immunfluorescence Staining in NHBE
April 13, 2015
This antibody was purchased to use as a marker of goblet cells in airway epithelial cells.
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E-cadherin / CDH1 Antibody Immunofluorescence
April 10, 2015
Cadherin formation is one of the steps among many in the process of airway epithelial cell differentiation. In our laboratory we ...
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ZO-1/TJP1 Immunofluorescence
April 09, 2015
In our laboratory we have to track airway epithelial cells throughout differentiation. Tight junction formation is among those ...
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Secondary Ab donkey anti Rabbit AF555
April 08, 2015
Secondary antibodies from Thermo Fisher Scientific are great for doing immunofluorescence. We work with primary cells (airway ...
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Product Review
Excellent Astrocyte Labeling with Low Background in Mouse Hippocampus
Ksenia Novototskaya
(2)
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We used this antibody to immunofluorescently label astrocytes in the mouse dorsal hippocampus following astrocyte-specific MOR knockdown. The signal was bright,...
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