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Cell Analysis
A Guide to Flow Cytometry
Flow Cytometers / Flow Cytometry Systems
SP6800 Spectral Analyzer
Supplier Images
Published Figures
SP6800 Spectral Analyzer
SP6800 Spectral Analyzer from Sony Corporation
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Citations:
(204)
Figures:
(2)
Inquire
from
Sony Corporation for
SP6800 Spectral Analyzer
Description
SP6800 spectral analyzer / flow cytometer uses patented technologies and spectral technology to optimize sensitivity, and simplify multicolor panel design.
Product Specs
Citations
Reviews
Product Specs
Item
SP6800 Spectral Analyzer
Company
Sony Corporation
Price
Inquire
Catalog Number
SP6800
Detection
FSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
Lasers
2 or 3 lasers
Excitation
405nm (60mW), 488nm (40mW), 638nm (60mW)
Parameters
64 fluorescence channels
Capacity
Tubes (5 mL)
Data Throughput
10,000 events/sec; 20,000 events/sec (max)
Dimensions
(WxDxH) 60.0 x 63.5 x 71.3 cm
Add to Compare List
Citations (204)
(161)
Time-Delayed Integration–Spectral Flow Cytometer (TDI-SFC) for Low-Abundance-Cell Immunophenotyping
Analytical Chemistry
April 2, 2019
Wenting Hu, Steven A. Soper, J. Matt Jackson
contributions from autofluorescence.3,8-12 Commercial SFC instruments from Cytek (Aurora) and
Sony
(
SP6800
) are available with the highest resolution provided by Sony, where a series of prisms disperses
fluorescence
(162)
Platelet GPIbα is a mediator and potential interventional target for NASH and subsequent liver cancer
Nature medicine
April 1, 2019
Malehmir M, Pfister D, Gallage S, Szydlowska M, Inverso D, Kotsiliti E, Leone V, Peiseler M, Surewaard BGJ, Rath D,
et al
.
were analyzed using BD FACS LSR II, BD FACS LSR Fortessa,
BD
FACSCanto
, and
Sony
spectral analyzer
SP6800
flow cytometers
, and data were analyzed using FlowJo.
Histology
,
immunohistochemistry
, scanning and
(163)
Beyond the message: advantages of snapshot proteomics with single‐cell mass cytometry in solid tumors
The FEBS Journal
April 1, 2019
Akshitkumar M. Mistry, Allison R. Greenplate, Rebecca A. Ihrie, Jonathan M. Irish
enhance conventional detection technology, and Cytek™ Aurora (Cytek Biosciences, Fremont, CA, USA) and
SP6800
(
Sony
Biotechnology, San Jose, CA, USA), which detect and deconvolute a known ‘spectral signature’ for
(164)
Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors
Science Immunology
March 22, 2019
Mar Cabeza-Cabrerizo et al.
content examination, samples were collected on a LSR Fortessa
flow cytometer
(BD Biosciences) or a
SP6800 Spectral Analyzer
(
Sony
) and analyzed using FlowJo 9 or 10 software (TreeStar Inc.). Gating strategies are shown in Fig.
(165)
Loss of ARHGEF1 causes a human primary antibody deficiency
The Journal of clinical investigation
March 1, 2019
Bouafia A, Lofek S, Bruneau J, Chentout L, Lamrini H, Trinquand A, Deau MC, Heurtier L, Meignin V, Picard C,
et al
.
lysing solution. Cells washed in PBS were then analyzed with a
MACSQuant analyzer
(
Miltenyi Biotec
) or an
SP6800 spectral analyzer
(
Sony
).
Cell culture
. PBMCs were obtained by Ficoll-Plaque
density gradient centrifugation
.
T cell
blasts
(166)
Persistence of Candida albicans in the Oral Mucosa Induces a Curbed Inflammatory Host Response That Is Independent of Immunosuppression
Frontiers in Immunology
February 27, 2019
Florian R. Kirchner, Katharina Littringer, Simon Altmeier, Van Du T. Tran, Franziska Schönherr, Christina Lemberg, Marco Pagni, Dominique Sanglard, Nicole Joller, Salomé LeibundGut-Landmann
in BD
Perm/Wash buffer
(BD Biosciences), Cells were acquired on a FACS Gallios (Beckman Coulter), a
SP6800 Spectral Analyzer
(
Sony
) or a
BD LSRFortessa
(
BD Biosciences
). The data were analyzed with FlowJo software (FlowJo LLC). The
(167)
Size-Dependent Effect of Silver Nanoparticles on the Tumor Necrosis Factor α-Induced DNA Damage Response
International Journal of Molecular Sciences
February 27, 2019
Alaa Fehaid, Akiyoshi Taniguchi
until analysis. The percentage of cells taking up AgNPs was analyzed using a
flow cytometer
(FACS,
SP6800 spectral analyzer
,
Sony
Biotechnology, Japan). Forward scatter (FSC) is the laser light scattered at narrow angles to
(168)
Interleukin-1/-33 Signaling Pathways as Therapeutic Targets for Endometriosis
Frontiers in immunology
January 1, 2019
Kato T, Yasuda K, Matsushita K, Ishii KJ, Hirota S, Yoshimoto T, Shibahara H.
BioLegend
) followed by streptavidin (Brilliantviolet 421,
405225
,
BioLegend
). Data were acquired using
SP6800
flow cytometer
(
SONY
, Tokyo, Japan) and analyzed using FlowJo software (Tree Star Inc., OR). Histological
(169)
PEGylated TiO
2
nanoparticles mediated inhibition of cell migration via integrin beta 1
Science and Technology of Advanced Materials
December 31, 2018
Qingqing Sun, Koki Kanehira, Akiyoshi Taniguchi
maintain cellular viability. Finally, the FITC intensity of the NPs was detected using a cell analyzer (
SP6800
,
Sony
, Tokyo, Japan) to evaluate differences in cellular uptake. 2.6.
Flow cytometry
assessment of
(170)
Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α
Science and Technology of Advanced Materials
December 31, 2018
Alaa Fehaid, Akiyoshi Taniguchi
until analysis. The percentage of cells taking up AgNPs was analyzed using a
flow cytometer
(FACS,
SP6800 spectral analyzer
,
Sony
Biotechnology, Japan). Forward scatter (FSC) is the laser light scattered at narrow angles to
(171)
Memory formation and long-term maintenance of IL-7Rα+ ILC1s via a lymph node-liver axis
Nature Communications
November 19, 2018
Xianwei Wang, Hui Peng, Jingjing Cong, Xuefu Wang, Zhexiong Lian, Haiming Wei, Rui Sun, Zhigang Tian
Staining
Kit (eBioscience) was used.
Flow cytometry
was performed on an LSR II (BD Biosciences) or
Sony
SP6800 Spectral Analyzer
(Supplementary Fig 6). Data were analyzed using the FlowJo software (Tree Star).
Cell isolation
Liver
(172)
Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation
Mucosal immunology
November 1, 2018
Tastan C, Karhan E, Zhou W, Fleming E, Voigt AY, Yao X, Wang L, Horne M, Placek L, Kozhaya L,
et al
.
All
flow cytometry analysis
was performed using LSR Fortessa X-20
flow cytometer
(BD Biosciences) or
SP6800
(
Sony
)
flow cytometer
and analyzed using FlowJo software (Tree Star). Preparation of
bacteria
supernatants Bacterial
(173)
Antigen-selective modulation of AAV immunogenicity with tolerogenic rapamycin nanoparticles enables successful vector re-administration
Nature communications
October 5, 2018
Meliani A, Boisgerault F, Hardet R, Marmier S, Collaud F, Ronzitti G, Leborgne C, Costa Verdera H, Simon Sola M, Charles S,
et al
.
antibodies were used at one test per 106 cells. Samples were acquired using Sony Spectral cell analyzer
SP6800
(
Sony
Biotechnology Inc., San Jose, CA). Data analysis was performed using the
SP6800
software (Sony
(174)
Radiation and Local Anti-CD40 Generate an Effective in situ Vaccine in Preclinical Models of Pancreatic Cancer
Frontiers in Immunology
September 7, 2018
Sayeda Yasmin-Karim, Patrick T. Bruck, Michele Moreau, Sijumon Kunjachan, Gui Zhen Chen, Rajiv Kumar, Stephanie Grabow, Stephanie K. Dougan, Wilfred Ngwa
micron cell strainer. Cell preparations were stained and analyzed using a
Sony
spectral cytofluorimeter (
SP6800
).
Flow cytometry
antibodies used in Female 6–8 week old C57BL/6J
mice
purchased from Jackson labs and
(175)
Von Hippel-Lindau Protein Is Required for Optimal Alveolar Macrophage Terminal Differentiation, Self-Renewal, and Function
Cell Reports
August 14, 2018
Helena M. Izquierdo, Paola Brandi, Manuel-José Gómez, Ruth Conde-Garrosa, Elena Priego, Michel Enamorado, Sarai Martínez-Cano, Iria Sánchez, Laura Conejero, Daniel Jimenez-Carretero,
et al.
cocktail in cold
flow cytometry
buffer. Samples were processed with a Spectral Analyzer
flow cytometer
(
SP6800
,
Sony
), and data were analyzed with FlowJo software (Tree Star). Additional information can be found
(176)
Dual HDAC and PI3K Inhibition Abrogates NFκB- and FOXM1-Mediated DNA Damage Response to Radiosensitize Pediatric High-Grade Gliomas
Cancer Research
July 15, 2018
Sharmistha Pal et al.
propidium iodide solution (Cell Signaling Technology). Cells were analyzed by
flow cytometry
(
Sony
SP6800
) and cell-cycle profiles were determined using the MODFIT software. Apoptosis was measured using the
(177)
[Fiat Lux. May be no more true in cytometry! Go to mass and spectrum but still stay classic]
médecine/sciences
June 15, 2018
Thierry Idziorek, Julie Cazareth, Catherine Blanc, Nathalie Jouy, Pierre Bourdely, Aurélien Corneau
cytomètre en flux utilisant la technologie d’analyse spectrale a été commercialisé en 2014 (sous le nom de
SP6800
par
SONY
Biotechnologies). Un schéma descriptif de ce cytomètre est présenté dans la Figure 3A. Dans
(178)
Flow Cytometry Data Preparation Guidelines for Improved Automated Phenotypic Analysis
The Journal of Immunology
May 15, 2018
Daniel Jimenez-Carretero, José M. Ligos, María Martínez-López, David Sancho, María C. Montoya
software tool without further adjustment. Data from the spectral
flow cytometer
were analyzed with
SP6800
software (
Sony
Biotechnology). Raw spectral data in comma-separated value format were obtained using
(179)
Dual HDAC and PI3K Inhibition Abrogates NFκB- and FOXM1-Mediated DNA Damage Response to Radiosensitize Pediatric High-Grade Gliomas
Cancer Res.
May 14, 2018
Sharmistha Pal, David Kozono, Xiaodong Yang, Wojciech Fendler, Whitney Fitts, Jing Ni, John A. Alberta, Jean Zhao, Kevin X. Liu, Jie Bian,
et al.
propidium iodide solution (Cell Signaling Technology). Cells were analyzed by
flow cytometry
(
Sony
SP6800
) and cell-cycle profiles were determined using the MODFIT software. Apoptosis was measured using the
(180)
Regulatory T cells with superior immunosuppressive capacity emigrate from the inflamed colon to draining lymph nodes
Mucosal Immunology
March 1, 2018
Y Nakanishi, R Ikebuchi, T Chtanova, Y Kusumoto, H Okuyama, T Moriya, T Honda, K Kabashima, T Watanabe, Y Sakai,
et al.
were acquired using Fortessa (BD MucosalImmunology | VOLUME 11 NUMBER 2 | MARCH 2018 Bioscience) or
SP6800
(
SONY
, Tokyo, Japan). Using Fortessa, KikGR green and red signals were detected using 530/60 and 595/50
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