SP6800 Spectral Analyzer from Sony Corporation

Product Specs
ItemSP6800 Spectral Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberSP6800
DetectionFSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
Lasers2 or 3 lasers
Excitation405nm (60mW), 488nm (40mW), 638nm (60mW)
Parameters64 fluorescence channels
CapacityTubes (5 mL)
Data Throughput10,000 events/sec; 20,000 events/sec (max)
Dimensions(WxDxH) 60.0 x 63.5 x 71.3 cm

(141) Role of Epstein-Barr Virus in Pathogenesis and Racial Distribution of IgA NephropathyFrontiers in ImmunologyFebruary 28, 2020Katerina Zachova, Petr Kosztyu, Josef Zadrazil, Karel Matousovic, Karel Vondrak, Petr Hubacek, Bruce A. Julian, Zina Moldoveanu, Zdenek Novak, Klara Kostovcikova, et al.antibody used by FACS Canto II flow cytometer; (B) Usage of fluorophore-labeled antibodies used by SONY SP6800 flow cytometer. Option 1 Fluorophore Option 2 Antigen Fluorophore Option 3 Antigen Fluorophore Option

(142) Inhibition of Haspin kinase promotes cell-intrinsic and extrinsic anti-tumor activityCancer ResearchFebruary 15, 2020Johannes C. Melms et al.intracellular IFNγ was stained using anti-IFNγ-PerCp-Cy5.5 (XMG1.2, Biolegend) Cells were acquired with a Sony SP6800 Spectral Flow Cytometer (Sony, Tokyo, Japan) and the data were analyzed using FlowJo 10.5.3 (TreeStar,

(143) Neoleukin-2 enhances anti-tumour immunity downstream of peptide vaccination targeted by an anti-MHC class II VHHOpen biologyFebruary 1, 2020Crowley SJ, Bruck PT, Bhuiyan MA, Mitchell-Gears A, Walsh MJ, Zhangxu K, Ali LR, Jeong HJ, Ingram JR, Knipe DM, et al.anti-CD8 and TRP1 tetramer (NIH tetramer core facility) and analysed by flow cytometry using a Sony SP6800 spectral flow cytometer. CFSE-dim cells were gated by the number of cell divisions and the proliferation

(144) Programmed 'disarming' of the neutrophil proteome reduces the magnitude of inflammationNature immunologyFebruary 1, 2020Adrover JM, Aroca-Crevillén A, Crainiciuc G, Ostos F, Rojas-Vega Y, Rubio-Ponce A, Cilloniz C, Bonzón-Kulichenko E, Calvo E, Rico D, et al.data from human samples. Cytometry and cell sorting Cytometric analyses were performed using a Sony SP6800 Spectral Analyzer (Sony Biotechnology). Analysis was performed using FlowJo v.X (Tree Star). Cell sorting experiments

(145) The Effects of TiO2 Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell LinesInternational Journal of Molecular SciencesJanuary 17, 2020Basma Salama, El-Said El-Sherbini, Gehad El-Sayed, Mohamed El-Adl, Koki Kanehira, Akiyoshi Taniguchithen cellular internal granularity was assessed using SSC and cell size was assessed using FSC using a SP6800 spectral analyzer (Sony Biotechnology, Tokyo, Japan). The percentage of cells containing nanoparticles was calculated

(146) CCR2 inhibition reduces tumor myeloid cells and unmasks a checkpoint inhibitor effect to slow progression of resistant murine gliomasProceedings of the National Academy of Sciences of the United States of AmericaJanuary 14, 2020Flores-Toro JA, Luo D, Gopinath A, Sarkisian MR, Campbell JJ, Charo IF, Singh R, Schall TJ, Datta M, Jain RK, et al.using single-color compensation on either a BD LSR Fortessa flow cytometer (BD Biosciences) or a Sony SP6800 spectral analyzer and quantified using FCS Express software (De Novo Software). Statistical Analysis. Student t test was

(147) MDM2-P53 Signaling Pathway-Mediated Upregulation of CDC20 Promotes Progression of Human Diffuse Large B-Cell LymphomaOncoTargets and therapyJanuary 1, 2020Sun C, Li M, Feng Y, Sun F, Zhang L, Xu Y, Lu S, Zhu J, Huang J, Wang J, et al.Iodide (PI) for 15 min in dark at room temperature. The stained cells were analyzed by flow cytometry (SP6800; Sony, Tokyo, Japan) for within 1 h. For cell cycle, the cells were suspended in a pre-cooling 70% alcohol

(148) Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated MiceFrontiers in immunologyJanuary 1, 2020Fisher E, Padula L, Podack K, O'Neill K, Seavey MM, Jayaraman P, Jasuja R, Strbo N.tumor necrosis factor alpha (TNFα): Clone: MPG-XT22. Data were collected on Spectral analyzer SONY SP6800 instrument (Sony Biotechnologies, Inc, San Jose, CA, USA). Analysis was performed using FlowJo™ software

(149) IL-23 supports host defense against systemic Candida albicans infection by ensuring myeloid cell survivalPLOS PathogensDecember 30, 2019Selim Nur, Florian Sparber, Christina Lemberg, Eva Guiducci, Tiziano A. Schweizer, Pascale Zwicky, Burkhard Becher, Salomé LeibundGut-Landmannstaining steps were carried out on ice. Cells were analyzed on a FACS Gallios (Beckman Coulter), Sony SP6800 Spectral Analyzer (Sony Biotechnology) or FACS LSR II Fortessa (BD Biosciences) and the data were analyzed with FlowJo

(150) Inhibition of Haspin kinase promotes cell-intrinsic and extrinsic anti-tumor activityCancer Res.December 27, 2019Johannes C. Melms, Sreeram Vallabhaneni, Caitlin E. Mills, Clarence Yapp, Jia-Yun Chen, Eugenio Morelli, Patricia Waszyk, Sushil Kumar, Derrick Deming, Nienke Moret, et al.intracellular IFNγ was stained using anti-IFNγ-PerCp-Cy5.5 (XMG1.2, Biolegend) Cells were acquired with a Sony SP6800 Spectral Flow Cytometer (Sony, Tokyo, Japan) and the data were analyzed using FlowJo 10.5.3 (TreeStar,

(151) Pancreatic nerve electrostimulation inhibits recent-onset autoimmune diabetesNature BiotechnologyDecember 1, 2019Mélanie Guyot, Thomas Simon, Franck Ceppo, Clara Panzolini, Alice Guyon, Julien Lavergne, Emilie Murris, Douglas Daoudlarian, Romain Brusini, Hadi Zarif, et al.are 16 from BD Biosciences. Dead cells were excluded using 7-AAD staining. Data were acquired on 17 SP6800 (Sony) flow cytometer and analyzed using Kaluza software. 18 RT-PCR: RNAs from pancreas were isolated

(152) How to make usage of the standardized EuroFlow 8-color protocols possible for instruments of different manufacturersJournal of Immunological MethodsDecember 1, 2019Michaela Nováková et al.calibration particles. Evaluation of spectral differences among fluorochromes. Spectral differences of selected fluorochromes and emission filters were tested on the Sony SP6800 flow cytometer (Sony Biotechnology Inc., Tokyo, Japan) equipped with 405nm, 488nm and 638nm excitation

(153) TNF Receptor-Associated Factor 5 Limits Function of Plasmacytoid Dendritic Cells by Controlling IFN Regulatory Factor 5 ExpressionJournal of immunology (Baltimore, Md. : 1950)September 15, 2019Kobayashi S, Sakurai T, So T, Shiota Y, Asao A, Phung HT, Tanaka R, Kawabe T, Maruyama T, Kanno E, et al.REGULATES pDC FUNCTION BY CONTROLLING IRF5 EXPRESSION Data were acquired on a SPECTRAL CELL ANALYZER SP6800 (Sony) or a FACSCanto II (BD Biosciences) and were analyzed with FlowJo software (Tree Star). Statistical

(154) Human organotypic brain slice culture: a novel framework for environmental research in neuro-oncologyLife Science AllianceAugust 1, 2019Vidhya M Ravi et al.resuspended in at least 0.5 to 1 ml of FACS buffer depending on the number of cells. We used a Sony SP6800 Spectral Analyzer and recorded 100,000 events per sample in standardization mode with PMT voltage set to maximum to reach

(155) Combination therapy targeting both innate and adaptive immunity improves survival in a pre-clinical model of ovarian cancerJournal for immunotherapy of cancerJuly 30, 2019Hartl CA, Bertschi A, Puerto RB, Andresen C, Cheney EM, Mittendorf EA, Guerriero JL, Goldberg MS.inspection of intracellular cytokines and cytolytic molecules. Flow cytometry was performed on a Sony SP6800 Spectral Analyzer (Sony Biotechnology), and all antibodies were purchased from BioLegend, R&D Systmes, or Cell Signaling

(156) LC3-Associated Endocytosis Facilitates β-Amyloid Clearance and Mitigates Neurodegeneration in Murine Alzheimer's DiseaseCellJuly 25, 2019Heckmann BL, Teubner BJW, Tummers B, Boada-Romero E, Harris L, Yang M, Guy CS, Zakharenko SS, Green DR.the indicated, conjugated primary antibodies. For all experiments, cells were analyzed using a Sony SP6800 Spectral Analyzer (Sony). All analyses were performed using FlowJo v10.4 (Tree Star). Fluorescent compensation was performed

(157) Mouse HSA+ immature cardiomyocytes persist in the adult heart and expand after ischemic injuryPLOS BiologyJune 27, 2019Mariana Valente, Tatiana Pinho Resende, Diana Santos Nascimento, Odile Burlen-Defranoux, Francisca Soares-da-Silva, Benoit Dupont, Ana Cumano, Perpétua Pinto-do-ÓFlow cytometry data were acquired in a BD FACSCanto II (BD Bioscience), BD LSRFortessa (BD Bioscience), and Sony SP6800 analyzer (Sony, Japan) and analyzed with the FlowJo version 10.0.8 (BD Bioscience), Kaluza 1.5 (Beckman

(158) Tumor-associated reactive astrocytes aid the evolution of immunosuppressive environment in glioblastomaNature communicationsJune 11, 2019Henrik Heiland D, Ravi VM, Behringer SP, Frenking JH, Wurm J, Joseph K, Garrelfs NWC, Strähle J, Heynckes S, Grauvogel J, et al.and resuspended in at least 0.5 to 1 ml of FACS buffer depending on the number of cells. We used Sony SP6800 spectral analyzer and recorded 100,000 events per sample in standardization mode with PMT voltage set to maximum to reach

(159) Single organelle analysis to characterize mitochondrial function and crosstalk during viral infectionScientific reportsJune 11, 2019Schneider A, Kurz S, Manske K, Janas M, Heikenwälder M, Misgeld T, Aichler M, Weissmann SF, Zischka H, Knolle P, et al.final analysis concentration of 0.05 µg/µl. Samples were analysed using the Spectral Cell Analyzer SP6800 (Sony Biotechnology Inc, Japan). The sample flow rate was set to record about 1500 events per second.

(160) T-cell and B-cell perturbations identify distinct differences in HIV-2 compared with HIV-1-induced immunodeficiencyAIDSJune 1, 2019Bo L Hønge, Mikkel S Petersen, Sanne Jespersen, Candida Medina, David D S Té, Bertram Kjerulff, Thomas Engell-Sørensen, Tina Madsen, Alex L Laursen, Christian Wejse, et al.process is described in the supplementary section. Samples were analyzed on a Sony Spectral Cell Analyzer SP6800 (Sony Corporation, Tokyo, Japan) flow cytometer. The software SP6800 version 1.6.3.7151 was used for

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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer from Sony Corporation

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SP6800 Spectral Analyzer from Sony Corporation

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