SP6800 Spectral Analyzer from Sony Corporation

Product Specs
ItemSP6800 Spectral Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberSP6800
DetectionFSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
Lasers2 or 3 lasers
Excitation405nm (60mW), 488nm (40mW), 638nm (60mW)
Parameters64 fluorescence channels
CapacityTubes (5 mL)
Data Throughput10,000 events/sec; 20,000 events/sec (max)
Dimensions(WxDxH) 60.0 x 63.5 x 71.3 cm

(121) Monocytes promote UV‐induced epidermal carcinogenesisEuropean Journal of ImmunologyJuly 1, 2021Iva Lelios, Sebastian A. Stifter, Virginia Cecconi, Ekaterina Petrova, Mirjam Lutz, Dilay Cansever, Sebastian G. Utz, Burkhard Becher, Maries van den Broek, Melanie GreterBioLegend), and TNF‐a (MP6‐XT22; BioLegend). Data were acquired on BD LSRFortessa, BD FACSymphony (BD), SP6800 (Sony Biotechnologies), or Cytek Aurora. Fluorescence‐activated cell sorting (FACS) was performed on

(122) ISL1 promoted tumorigenesis and EMT via Aurora kinase A-induced activation of PI3K/AKT signaling pathway in neuroblastomaCell Death & DiseaseJune 1, 2021Mengzhen Li et al.Annexin V-FITC for 15 min in the dark. Analysis of the stained cells was performed with flow cytometry (SP6800, Sony, Japan) in 1 h. Xenograft tumorigenesis in vivo NOD/SCID mice at 3–4 weeks of age were used

(123) cIAP1/2 antagonism eliminates MHC class I–negative tumors through T cell–dependent reprogramming of mononuclear phagocytesScience Translational MedicineMay 19, 2021Kevin Roehle et al.in PBS, and resuspended in 1% formalin prior to analysis on either a spectral flow cytometer (Sony SP6800) or a Fortessa cytofluorimeter (BD). Flow cytometry antibodies used in this study were purchased from

(124) In utero and early-life exposure to thirdhand smoke causes profound changes to the immune systemClinical ScienceApril 30, 2021Antoine M. Snijders et al./Gr-1mod-hi )]. Antibody details are provided in Supplementary Table S4. The cells were analyzed on a SP6800 spectral analyzer (Sony Biotechnology Inc.) and the percentages of cell populations were delineated with FlowJo software. A

(125) Correction of β-thalassemia by CRISPR/Cas9 editing of the α-globin locus in human hematopoietic stem cellsBlood AdvancesMarch 9, 2021Giulia Pavani et al.manufacturers’ instructions. Cells were analyzed using CytoFLEX S (Beckman Coulter, Pasadena, CA) or SP6800 Spectral Analyzer (Sony, Tokyo, Japan); data were elaborated with CytExpert (Beckman Coulter) or FlowJo software (Tree

(126) Tissue-resident memory Th17 cells maintain stable fungal commensalism in the oral mucosaMucosal immunologyMarch 1, 2021Kirchner FR, LeibundGut-Landmann S.Permeabilization buffer (eBioscience). Cells were acquired on a FACS Gallios (Beckman Coulter) or a SP6800 Spectral Analyzer (Sony). Data were analyzed with FlowJo software (FlowJo LLC). The gating of the flow cytometric data

(127) Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced ArthritisMediators of InflammationFebruary 20, 2021Fengfan Yang, Xing Luo, Wenxiao Zhu, Jia Li, Zhaohui Zheng, Ping Zhu(B2D) were added for transcription factor staining. The processed cells were analyzed using a SONY SP6800 Spectral Analyzer or a Beckman Coulter-CytoFLEX flow cytometer and corresponding software. 2.5. Detection of Cytokines

(128) Mucosal-Associated Invariant T (MAIT) Cells Are Highly Activated and Functionally Impaired in COVID-19 PatientsVirusesFebruary 3, 2021Deschler S, Kager J, Erber J, Fricke L, Koyumdzhieva P, Georgieva A, Lahmer T, Wiessner JR, Voit F, Schneider J, et al.Fixation and Permeabilisation Buffer Set (Thermo Fisher, Waltham, MA, USA). Samples were acquired with SP6800 Spectral Analyzer (Sony, Tokyo, Japan) and analysed with FlowJo 10.7 (BD Biosciences, San Jose, CA, USA). Table 1. Characteristics

(129) Structurally symmetric near-infrared fluorophore IRDye78-protein complex enables multimodal cancer imagingTheranosticsJanuary 1, 2021Jiang Yang et al.FortessaTM X-20. Spectral flow cytometry was performed on suspended cells using 638 nm laser with the SONY SP6800 Spectral Cell Analyzer. Zeta potentials of α-LA before and after covalent modification were measured

(130) Acute Hemolysis and Heme Suppress Anti-CD40 Antibody-Induced Necro-Inflammatory Liver DiseaseFrontiers in immunologyJanuary 1, 2021Pfefferlé M, Ingoglia G, Schaer CA, Hansen K, Schulthess N, Humar R, Schaer DJ, Vallelian F.(2 µg/mL, Stemcell #60033) antibody. Stained cells were analyzed using the LSRFortessa (BD) or the SP6800 Spectral Analyzer (Sony). Data were analyzed using FlowJo and FCS express 6 (De Novo software). Gene Expression Analysis Microarray

(131) Type 2 immunity is maintained during cancer-associated adipose tissue wastingImmunother AdvJanuary 1, 2021Lenehan PJ, Cirella A, Uchida AM, Crowley SJ, Sharova T, Boland G, Dougan M, Dougan SK, Heckler M.ml cold PBS. After the final wash, samples were fixed in 1% formalin (in PBS) and analyzed on a Sony SP6800 spectral flow cytometer. Analysis and quantification of flow cytometry data was performed in FlowJo

(132) RXRs control serous macrophage neonatal expansion and identity and contribute to ovarian cancer progressionNature CommunicationsDecember 1, 2020MarÃa Casanova-Acebes, MarÃa Piedad MenÃ©ndez-GutiÃ©rrez, JesÃºs Porcuna, Damiana Ãlvarez-Errico, Yonit Lavin, Ana GarcÃa, Soma Kobayashi, Jessica Le Berichel, Vanessa NÃºÃ±ez, Felipe Were, et al.PBS 1×. Flow cytometry was performed using Fortessa (BD, Biosciences), Canto 3 L (BD, Biosciences) or SP6800 Spectral Analyzer (SONY) and data were analysed using FlowJo 10.4.2 Software. For sorting, we used an ARIA SORT (BD, Biosciences)

(133) Vaccination against RhoC induces long-lasting immune responses in patients with prostate cancer: results from a phase I/II clinical trialJournal for ImmunoTherapy of CancerNovember 12, 2020Juliane Schuhmacher et al.staining, cells were washed twice, resuspended in FACS buffer, and acquired on a Spectral cell analyzer SP6800 (Sony) using the software V.2.0.2.14140. Analysis was done with FlowJo (V.10.6.2). Identification of

(134) Method for the Analysis of the Tumor Microenvironment by Mass Cytometry: Application to Chronic Lymphocytic LeukemiaFrontiers in ImmunologyOctober 20, 2020Susanne Gonder, Iria Fernandez Botana, Marina Wierz, Giulia Pagano, Ernesto Gargiulo, Antonio Cosma, Etienne Moussay, Jerome Paggetti, Anne Largeotin parallel (such as the BDSymphony or bio-rad ZE5), and the emergence of spectral cytometers (Sony SP6800 and Cytek Aurora) positioning both technologies at the forefront of immunology research. However, the

(135) EGF Conjugation Improves Safety and Uptake Efficacy of Titanium Dioxide NanoparticlesMoleculesSeptember 29, 2020Basma Salama, Chia-Jung Chang, Koki Kanehira, El-Said El-Sherbini, Gehad El-Sayed, Mohamed El-Adl, Akiyoshi Taniguchiassessed using side-scattered light and cell size was assessed using forward-scattered light using a SP6800 spectral analyzer (Sony Biotechnology, Tokyo, Japan). The percentage of cells incorporated with nanoparticles was calculated

(136) Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After CoitusFrontiers in ImmunologySeptember 11, 2020Ippei Yasuda, Tomoko Shima, Taiki Moriya, Ryoyo Ikebuchi, Yutaka Kusumoto, Akemi Ushijima, Akitoshi Nakashima, Michio Tomura, Shigeru Saitosupernatant to block Fc binding. Dead cells were labeled with PI. Stained samples were acquired using SP6800 (SONY, Tokyo, Japan). KikGR-green and red signals were detected using 530/60 and 595/50 bandpass filters,

(137) Costello syndrome model mice with a HrasG12S/+ mutation are susceptible to develop house dust mite-induced atopic dermatitisCell Death & DiseaseAugust 13, 2020Yu Katata et al.antibodies described in Supplementary Table 2. Flow cytometry data were acquired by Spectral Analyzer (SP6800; SONY, Tokyo, Japan) and analyzed with FlowJo (Becton, Dickinson and Company, USA). Spleen and LN immune

(138) Molecular Pathways of Colon Inflammation Induced by Cancer ImmunotherapyCellAugust 6, 2020Luoma AM, Suo S, Williams HL, Sharova T, Sullivan K, Manos M, Bowling P, Hodi FS, Rahma O, Sullivan RJ, et al.flow cytometry analysis Stained cells were passed through a 40 μm filter and analyzed with a Sony SP6800 Spectral Analyzer. Single-color controls for spectral unmixing, using the identical antibodies as for analysis, were acquired

(139) Ex vivo editing of human hematopoietic stem cells for erythroid expression of therapeutic proteinsNature communicationsJuly 29, 2020Pavani G, Laurent M, Fabiano A, Cantelli E, Sakkal A, Corre G, Lenting PJ, Concordet JP, Toueille M, Miccio A, et al.manufacturers’ instructions. Cells were analyzed using CytoFLEX S (Beckman Coulter, Pasadena, CA, USA) or SP6800 Spectral Analyzer (Sony, Tokyo, Japan); data were elaborated with CytExpert (Beckman Coulter, Pasadena, CA, USA) or FlowJo

(140) Effect of immune regulatory pathways after immunization with GMZ2 malaria vaccine candidate in healthy lifelong malaria-exposed adultsVaccineJune 1, 2020Odilon Nouatin, Ulysse Ateba Ateba Ngoa, Javier Ibáñez, Jean Claude Dejon-Agobe, Benjamin Mordmüller, Jean Ronald Edoa, Fabrice Mougeni, Sina Brückner, Aurore Bouyoukou Bouyoukou Hounkpatin, Meral Esen, et al.manufacturer’s recommendations (BD Biosciences). Cell acquisition was performed using a Spectral Cell Analyzer SP6800 (SONY) 15-color cytometer, with 100,000 cells per tube as the total number of cells acquired. Treg,

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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer from Sony Corporation

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SP6800 Spectral Analyzer from Sony Corporation

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