Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Functional analyses identify the L1 ORF2p Cryptic domain as a hub for intramolecular interfaces required for retrotranspositionNucleic Acids ResApril 23, 2026Afzaal M Shareef, Guizi Chen, Claiborne M Christian, Benjamin Freeman, Dawn LaCoste, Shuaihua Gao, Edgar Africano Camargo, Jude N Ubi, Victoria P Belanciogenerated using ∼50 ng of eluted mRNA, the Avian Myeloblastosis Virus (AMV) RT-PCR kit (Promega, Cat #A3500) and an Oligo dT 30 primer (5′-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-3′). The cDNA was used in PCR with Oligo

(2) TGF-β and IL-4 + IL-13 induce neuroplasticity in an in vitro model of hPSC-derived sensory neuronsFront ImmunolMarch 3, 2026Carli S. Koster, I. Sophie T. Bos, Chiara Lavitola, Mihaly Balogh, Barbro N. Melgert, Reinoud GosensGSE307973. RT-qPCR For RT-qPCR, cDNA was synthesized using the Reverse Transcription System kit (#A3500, Promega,Netherlands), following the manufacturer’s instructions. qRT-PCR was performed using SYBR Green

(3) Hydrogen Sulfide Inhibits H. pylori-Induced Gastric Fibroblast Activation: Implications for Cancer PreventionCellsJanuary 16, 2026Gracjana Krzysiek-Maczka, Aneta Targosz, Patrycja Bronowicka-Adamska, Urszula Szczyrk, Malgorzata Strzalka, Hubert Mączka, Mateusz Wierdak, Izabela Rodzon, Jaroslaw Czyz, Tomasz Brzozowski, et al.Scientific, Carlsbad, CA, USA) [6], quantified with Nanodrop ND-1000, and reverse-transcribed using Promega A3500 kit. Expression for Twist, IL-6, STAT3, IL-8, FAP, HGF, CBS, MPST, TST, Ki67 was determined using specific

(4) P2RX1 Influences the Prognosis of Ph+/Ph-Like ALL through Energy and Calcium MetabolismOncology ResearchDecember 30, 2025Xiangmei Ye, Baoyi Yang, Xin Zhang, Luyuan Yang, Likun Zhang, Qin Ren, Xiaobing Li, Leiguang Feng, Lanlan Wei, Peng Song, et al.spectrophotometer. Subsequently, 1 μg of total RNA was reverse-transcribed into cDNA (Reverse Transcription System(A3500), Promega, Madison, Wisconsin, USA). Finally, sequencing libraries were constructed and analyzed on

(5) The monkeypox virus suppresses autophagy by modulating Rubicon expressionCell Death DiscoveryDecember 23, 2025Giulia Refolo, Cosmina Mija, Fabiola Ciccosanti, Giuseppe Sberna, Valentina Mazzotta, Fabrizio Maggi, Mauro Piacentini, Tiziana Vescovo, Licia BordiComplementary DNA synthesis was generated from 2 μg of RNA using the reverse transcription kit (Promega, A3500) according to the manufacturer’s recommendations. Real-time polymerase chain reactions (PCRs) were performed

(6) Targeting ATR offers multifaceted treatment strategies involving RAD51-mediated compensatory DNA repair in bladder cancerJournal of Experimental & Clinical Cancer ResearchDecember 12, 2025Julia Pannhausen, Ahmed A. Chughtai, Cem-Louis Yüce, Michael K. Melzer, Yanchun Ma, Lancelot Seillier, Emiel P. C. van der Vorst, Geoffroy Andrieux, Julia Wirtz, Sophie Leypold, et al.reverse transcription PCR cDNA synthesis was performed using the Reverse Transcription System Kit (A3500, Promega) with 1 µg total RNA on a Bio-Rad C1000 Touch Thermal Cycler. Mastermix components and cycling

(7) Inhibition of IRAK4 by microbial trimethylamine blunts metabolic inflammation and ameliorates glycemic controlNat MetabDecember 8, 2025Julien Chilloux, Francois Brial, Amandine Everard, David Smyth, Petros Andrikopoulos, Liyong Zhang, Hubert Plovier, Antonis Myridakis, Lesley Hoyles, José Maria Moreno-Navarrete, et al.isoflurane (10014451, Forene, Abbott); TriPure reagent (1667165, Roche); Reverse Transcription System kit (A3500, Promega); Mesa Fast qPCR (CS-CKIT-PROD, Eurogentec); and the MSD V-PLEX Plus Proinflammatory Panel

(8) OPTN deficiency through CRISPR/Cas9 downregulates autophagy and mitophagy in a SOD1-G93A-expressing transgenic cell lineIBRO Neuroscience ReportsDecember 1, 2025Di Wen, Qiusheng Li, Yuanyuan Li, Wenyu Yan, Yanyan Wang, Yakun LiuTRIzol reagent (Qiagen, Hilden, Germany). cDNA was synthesized through a reverse transcription system (A3500, Promega) with template RNA. A quantitative real-time PCR assay was performed with Power qPCR PreMix

(9) Development of a Hepatocyte‐Specific Temporal Genetic Mouse Model Using Albumin Promoter‐Driven FlpER2 ExpressiongenesisNovember 9, 2025Bin Wang, Jiale Wang, Yang Liu, Gordon He, Yasmin Jahan‐Mihan, Yuxi Wang, Jie Hu, Audrey Li, Faheem Muhammad, Yan Bi, et al.No.: 74104). cDNA synthesis was performed using the Reverse Transcription System (Promega, Cat. No.: A3500). Genomic DNA extracted from the liver, lung and pancreas with DNA kit (Qiagen, Cat. No.:80204). For

(10) A natural allele of PC08 lost in domestication contributes to soybean seed storage protein accumulationProceedings of the National Academy of SciencesNovember 4, 2025Shu Liu, Peichun Zeng, Dingyue Ban, Chunyu Zhang, Fanjiang Kong, Xiaoming Li, Xingliang Hou(LS1040, Promega). Complementary DNA (cDNA) was synthesized with a Promega reverse transcription kit (A3500, Promega). RT-qPCR was performed with the KAPA SYBR FAST qPCR Kit (KK4601, Sigma-Aldrich, USA) using

(11) Terminalia chebula Retz. Fruit Extract Promotes Murine Hair Growth by Suppressing 5α-Reductase and Accelerating the Degradation of DihydrotestosteroneBiomedicinesOctober 22, 2025Ting Cui, Xiaoqing Wang, Qi Wu, Ye Zhong, Fenglou Wang, Yue Zou, Yushu Wang, Shanshan Jiang, Gang MaReal-Time PCR Briefly, 1 µg of total RNA was transcribed by using Reverse Transcriptase Kit (Promega, A3500) following the manufacturer’s instructions. Real-time PCR was conducted by using the Roche lightcycle

(12) Human platelets support Semliki Forest virus replicationBMC Research NotesSeptember 30, 2025Cameron J. Stockwell, Matthew S. Hindlefrom Invitrogen. Direct-zol RNA MiniPrep Plus (R2072) was from Zymo. Reverse transcription system (A3500) was from Promega. Itaq Universal SYBR® Green Master mix (1725120) was from Bio-Rad. All other reagents

(13) Integrated genomics reveals cellular senescence‐driven molecular networks and immune crosstalk in myopia pathogenesisJournal of Cell Communication and SignalingSeptember 21, 2025Wangming Su, Pinsheng Qiu, Yanling Li, Ping Xie, Xiaoyong Yuanreagent (Magen). The RNA (1 μg) is reverse transcribed into cDNA using a reverse transcription system (A3500, Promega) with a reaction volume of 10 μL. Oligo(dT)15 primers (C110A, Promega) and random primers (C118A,

(14) A novel mouse model for LAMA2-related muscular dystrophy with analysis of molecular pathogenesis and clinical phenotypeeLifeSeptember 17, 2025Tan D, Liu Y, Luo H, Shen Q, Long X, Xu L, Liu J, Zhong NA, Zhang H, Xiong H.and dyH/dyH, and was reverse transcribed to first strand cDNA using the Reverse Transcription System (A3500, Promega, Madison, Wisconsin, USA). RT–PCR with the forward primer (5'-TGCTTCGAATGCACTCATCACAAC-3′)

(15) Identification and Phylogenetic Characterisation of Novel Adeno‐Associated Virus Capsids in Non‐Human Primate TissuesCell ProliferationSeptember 16, 2025Liyu Zhu, Kai Xu, Yali Ding, Kailun Liu, Jing Liu, Zongren Hou, Rui Niu, Ning Yang, Hualing Qin, Baoyang Hu, et al.to RNA purification (Invitrogen, 12183018A), followed by reverse transcription into cDNA (Promega, A3500). 2.3 AAV Genomic DNA Characterisation and Phylogenetic Reconstruction Nested PCR amplification was

(16) Arrestin-biased allosteric modulator of neurotensin receptor 1 alleviates acute and chronic painCellAugust 7, 2025Ran Guo, Ouyang Chen, Yang Zhou, Sangsu Bang, Sharat Chandra, Yize Li, Gang Chen, Rou-Gang Xie, Wei He, Jing Xu, et al.16894 Protease inhibitor cocktail,Millipore/Sigma,Cat# P8340 Reverse Transcription System,Promega,Cat # A3500 Coelenterazine 400a (hydrochloride),Cayman Chemical,Cat # 25738 Critical commercial assays,Critical

(17) Identifying pyroptosis-related genes as novel therapeutic targets in diabetic foot ulcerationDiabetol Metab SyndrAugust 1, 2025Kang Liu, Lei Lei, Xin-Lei Yang, Xin-He Zhang(Qiagen, 74106). cDNA synthesis was carried out using 1 µg of RNA and an RT reaction mixture (Promega, A3500). qPCR was performed on an I-Cycler and IQ real-time PCR system (Bio-Rad) with SYBR Green I. Gene expression

(18) Intrauterine growth-restricted (IUGR) female Yucatan miniature pig neonates fed parenteral nutrition exhibit early catch-up growth leading to obesity and ectopic fat deposition in adulthoodThe Journal of NutritionAugust 1, 2025Raniru S Randunu, Lee-Anne Huber, Janet A Brunton, Robert F Bertoloagarose gel. cDNA was produced from the extracted RNA samples using reverse transcription (catalog # A3500; Promega). Real-time qPCR primers (acetyl-CoA carboxylase (ACC-1), fatty acid synthase (FASN), sterol

(19) Which One Would You Choose?-Investigation of Widely Used Housekeeping Genes and Proteins in the Spinal Cord of an Animal Model of Amyotrophic Lateral SclerosisNeuroSciJuly 23, 2025Epplen ASC, Stahlke S, Theiss C, Matschke V.synthesis of cDNA was carried out using a Reverse Transcription System (Promega, Madison, WI, USA, Cat# A3500), as outlined in the manufacturer’s protocol, with 1 μg of tRNA and oligo(dT) primer. The resulting

(20) Differentiation to insulin-positive cells from human amnion epithelial cells using a pancreatic development mimicry protocolHistochem Cell BiolJuly 14, 2025Daniel Martínez-Rodríguez, Jonathan Salazar-Alonso, Axel Castro-Abrego, Daniela Ávila-González, Omar Martínez-Alarcón, Anayansi Molina-Hernández, Alejandro Martínez-Juárez, Héctor Salvador Godoy-Morales, Diana Elizabeth Lara-Barragán, Wendy Portillo, et al.was synthesized using the reverse transcription system (Promega Corporation, Madison, Wisconsin, USA; A3500). A total of 800 ng of RNA was mixed with 5 mM MgCl2, 1× reverse transcription buffer (10 mM Tris-HCl,

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