Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Detection of Avian Orthoavulavirus-1 genotypes VI.2.1 and VII.1.1 with neuro-viscerotropic tropism in some backyard pigeons (Columbidae) in Eastern Saudi ArabiaFrontiers in Veterinary ScienceFebruary 27, 2024Abdullah I. A. Al-Mubarak, Anwar A. G. Al-Kubati, Abdullah Sheikh, Adel M. Abdelaziz, Jamal Hussen, Mahmoud Kandeel, Baraa Falemban, Maged Gomaa HemidaAOAV1 amplicons According to the manufacturer's instructions, the Reverse Transcription System (Cat # A3500, Promega, USA) was used to reverse transcribe of the extracted viral RNA. The obtained cDNA was then

(62) Mechanisms of mesothelial cell response to viral infections: HDAC1-3 inhibition blocks poly(I:C)-induced type I interferon response and modulates the mesenchymal/inflammatory phenotypeFrontiers in Cellular and Infection MicrobiologyFebruary 27, 2024Flavia Trionfetti, Claudia Montaldo, Ivan Caiello, Giulio Bontempi, Michela Terri, Marta Tiberi, Vanessa Marchant, Alessandro Domenici, Paolo Menè, Marco Cordani, et al.to the manufacturer’s instructions. cDNA synthesis was generated using a reverse transcription kit (A3500) from Promega (Madison, WI, USA), according to the manufacturer’s recommendations. cDNAs were amplified

(63) A multi-antigen vaccinia vaccine broadly protected mice against SARS-CoV-2 and influenza A virus while also targeting SARS-CoV-1 and MERS-CoVFrontiers in immunologyJanuary 1, 2024Gao N, Yang T, Dong L, Tang W, Cao K, Ding L, Zhu C, Bai S, Xia A, Zhu Y, et al.ZYMO Research, California, USA), followed by reverse transcription using a Reverse Transcription System (A3500, Promega). The resulting cDNAs were then amplified with the GoTaq qPCR Master Mix (A6002, Promega) on

(64) Inhibition of porcine reproductive and respiratory syndrome virus replication by rifampicin in vitroFrontiers in veterinary scienceJanuary 1, 2024Wei R, Li L, Chen H, Wang X, Chen Y, Liu X.instructions. Reverse transcription of 1.0 μg of RNAs was performed using the Reverse Transcription System (A3500, Promega, United States). The reverse-transcription products were amplified using 2 × RealStar Green

(65) HDAC1-3 inhibition increases SARS-CoV-2 replication and productive infection in lung mesothelial and epithelial cellsFront Cell Infect MicrobiolDecember 13, 2023Flavia Trionfetti, Tonino Alonzi, Giulio Bontempi, Michela Terri, Cecilia Battistelli, Claudia Montaldo, Federica Repele, Dante Rotili, Sergio Valente, Clemens Zwergel, et al.to the manufacturer’s instructions. cDNA synthesis was generated using a reverse transcription kit (A3500) from Promega (Madison, WI), according to the manufacturer’s recommendations. cDNAs were amplified by

(66) Hepatitis B virus infection disrupts homologous recombination in hepatocellular carcinoma by stabilizing resection inhibitor ADRM1J Clin InvestDecember 1, 2023Ming Zeng, Zizhi Tang, Laifeng Ren, Haibin Wang, Xiaojun Wang, Wenyuan Zhu, Xiaobing Mao, Zeyang Li, Xianming Mo, Jun Chen, et al.(Macherey-Nagel, 740404). cDNA was obtained from 1 μg RNA by Reverse Transcription System (Promega, A3500). Real-time quantitative PCR (qPCR) and semiquantitative PCR were performed on a Bio-Rad CFX96 Real

(67) Evaluating the Effect of Gestational Exposure to Perfluorohexane Sulfonate on Placental Development in Mice Combining Alternative Splicing and Gene Expression AnalysesEnviron Health PerspectNovember 23, 2023Yihao Zhang, Jia Lv, Yi-Jun Fan, Lin Tao, Jingjing Xu, Weitian Tang, Nan Sun, Ling-Li Zhao, De-Xiang Xu, Yichao Huanginstructions. Then, reverse transcription of RNA was performed using a Reverse Transcription system (A3500; Promega, USA) to obtain cDNA by Biometra T-Gradient (Biometra biomedizinische Analytik GmbH, Germany).

(68) Evaluating the Effect of Gestational Exposure to Perfluorohexane Sulfonate on Placental Development in Mice Combining Alternative Splicing and Gene Expression AnalysesEnvironmental Health PerspectivesNovember 23, 2023Yihao Zhang, Jia Lv, Yi-Jun Fan, Lin Tao, Jingjing Xu, Weitian Tang, Nan Sun, Ling-Li Zhao, De-Xiang Xu, Yichao Huanginstructions. Then, reverse transcription of RNA was performed using a Reverse Transcription system (A3500; Promega, USA) to obtain cDNA by Biometra T-Gradient (Biometra biomedizinische Analytik GmbH, Germany).

(69) Identification of new biomarkers and immune infiltration characteristics of sepsis in very low birth weight infantsBiomolecules and BiomedicineOctober 1, 2023Yujia Luo, Zhou Jiang, Rei Gu, Xuandong Zhang, Li Wei, Yuanyuan Zhou, Songying Zhangused to extract total RNA from HUVECs, which was then reverse transcribed to cDNA using an RT-PCR Kit (A3500, Promega, USA). For the real-time PCR, cDNA was combined with SYBR Green Master Mix (DBI-2044, Germany).

(70) Total Flavonoids of Rhizoma Drynariae Treat Osteoarthritis by Inhibiting Arachidonic Acid Metabolites Through AMPK/NFκB PathwayJournal of Inflammation ResearchSeptember 20, 2023Guang-Yao Chen, Xiao-Yu Liu, Xue-Er Yan, XinBo Yu, Yi Liu, Jing Luo, Qing-Wen TaoKit (H552-1, Nanjing Jiancheng Bioengineering Institute, China), cDNA Reverse Transcription System (A3500, Promega, USA), SYBR Green Realtime PCR Master Mix (QPK-201, Toyobo, Japan), Mitochondrial Membrane

(71) Hispidulin Inhibits the Vascular Inflammation Triggered by Porphyromonas gingivalis LipopolysaccharideMoleculesSeptember 20, 2023Yeon Kim, Hoyong Lee, Hyun-Joo Park, Mi-Kyoung Kim, Yong-Il Kim, Hyung Joon Kim, Soo-Kyung Bae, Yung-Jin Kim, Moon-Kyoung Bae(#301-001, GeneAll, Seoul, Republic of Korea) and reverse-transcribed with a reverse transcription kit (#A3500, Promega, Madison, WI, USA) according to the manufacturer’s instruction, followed by an RT-qPCR with

(72) Total Flavonoids of Rhizoma Drynariae Treat Osteoarthritis by Inhibiting Arachidonic Acid Metabolites Through AMPK/NFκB PathwayJ Inflamm ResSeptember 20, 2023Guang-Yao Chen, Xiao-Yu Liu, Xue-Er Yan, XinBo Yu, Yi Liu, Jing Luo, Qing-Wen TaoKit (H552-1, Nanjing Jiancheng Bioengineering Institute, China), cDNA Reverse Transcription System (A3500, Promega, USA), SYBR Green Realtime PCR Master Mix (QPK-201, Toyobo, Japan), Mitochondrial Membrane

(73) Elabela inhibits TRAF1/NF-κB induced oxidative DNA damage to promote diabetic foot ulcer wound healingiScienceSeptember 15, 2023Yinghui Hong, Jun Li, Yinsheng Zhong, Shujun Yang, Liying Pei, Zijie Huang, Xuxiang Chen, Hao Wu, Guanghui Zheng, Chaotao Zeng, et al.and real-time quantitative PCR The recommended protocol for the Promega Reverse Transcription Kit (#A3500, Promega, USA) was adopted. A total of 20 μl reaction system was prepared as guidance. The cDNA was

(74) BATF relieves hepatic steatosis by inhibiting PD1 and promoting energy metabolismeLifeSeptember 15, 2023Zhang Z, Liao Q, Pan T, Yu L, Luo Z, Su S, Liu S, Hou M, Li Y, Damba T, et al.(Life Technologies, USA). Reverse transcription of RNA was performed with the Reverse Transcription System (A3500, Promega), and formed cDNA from 1 μg total RNA. 2×RealStar Green Fast Mixture was used for real-time

(75) Effects of Gestational Arsenic Exposures on Placental and Fetal Development in Mice: The Role of Cyr61 m6AEnvironmental Health PerspectivesSeptember 8, 2023Ya-Ping Song, Jin-Wei Lv, Zhi-Cheng Zhang, Qing-Hua Qian, Yi-Jun Fan, Dao-Zhen Chen, Heng Zhang, Fei-Xiang Xu, Cheng Zhang, Yichao Huang, et al.treated with DNase without RNase and reverse-transcribed with Promega reverse transcription system (#A3500; Promega). Real-time RT-PCR was performed with a LightCycler 480 SYBR Green qPCR Master Mix (#04887352001;

(76) Effects of Gestational Arsenic Exposures on Placental and Fetal Development in Mice: The Role of Cyr61 m6AEnviron Health PerspectSeptember 8, 2023Ya-Ping Song, Jin-Wei Lv, Zhi-Cheng Zhang, Qing-Hua Qian, Yi-Jun Fan, Dao-Zhen Chen, Heng Zhang, Fei-Xiang Xu, Cheng Zhang, Yichao Huang, et al.treated with DNase without RNase and reverse-transcribed with Promega reverse transcription system (#A3500; Promega). Real-time RT-PCR was performed with a LightCycler 480 SYBR Green qPCR Master Mix (#04887352001;

(77) Comparative Analysis and Identification of Terpene Synthase Genes in Convallaria keiskei Leaf, Flower and Root Using RNA-Sequencing ProfilingPlants (Basel)July 28, 2023Sivagami-Jean Claude, Gurusamy Raman, Seon-Joo Parkand primer structure (Supplementary Table S1). For cDNA synthesis, a reverse transcription system (A3500, Promega (product made in USA), Yuseong-gu, Daejeon, Korea) was used, and 20 µL of the final cDNA product

(78) ALDH1A1 promotes PARP inhibitor resistance by enhancing retinoic acid receptor-mediated DNA polymerase θ expressionNPJ precision oncologyJuly 10, 2023Lavudi K, Banerjee A, Li N, Yang Y, Cai S, Bai X, Zhang X, Li A, Wani E, Yang SM, et al.total, 1 μg of RNA was used to synthesize cDNA using the Promega Reverse Transcription System (Cat. No. A3500, Promega, Madison, WI) in a 20 μl reaction. After diluted four times, 2.5 μl of cDNA was amplified by

(79) Bioinformatic Analysis and Machine Learning Methods in Neonatal Sepsis: Identification of Biomarkers and Immune InfiltrationBiomedicinesJune 28, 2023Zhou Jiang, Yujia Luo, Li Wei, Rui Gu, Xuandong Zhang, Yuanyuan Zhou, Songying Zhang(Qiagen 74034, Hilden, Germany), and this RNA was then reverse-transcribed into cDNA using an RT-PCR Kit (A3500, Promega, Madison, WI, USA). SYBR Green Master Mix (DBI-2044, Freiberg, Germany) was mixed with cDNA

(80) A LY6E-PHB1-TRIM21 assembly degrades CD14 protein to mitigate LPS-induced inflammatory responseiScienceJune 16, 2023Xinyu Zhu, Linxia Zhang, Daobin Feng, Lang Jiang, Peng Sun, Chen Zhao, Xiaoyan Zhang, Jianqing XuReverse transcription of RNA into cDNA was performed using Reverse Transcription System (Promega # A3500). The resultant cDNAs were then subjected to quantitative PCR analyses conducted on a 7300 real-time PCR

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