Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) Deficiency of lysophosphatidic acid receptor 3 decreases erythropoietin production in hypoxic mouse kidneysLipids in Health and DiseaseNovember 18, 2024Nan Yin, Xuyuan Li, Di Zhang, Mengxia Qu, Shengqiang Pei, Xi Chen, Xiaotian Zhang, Junjie Zhangreverse transcribed by using anchored oligo dT primers and the Reverse Transcription System (Cat No: A3500, Promega Biotech, WI, USA). cDNA was amplified with the specific primers listed in Table S1 via real-time

(42) Digoxin and exercise effects on skeletal muscle Na+,K+‐ATPase isoform gene expression in healthy humansExperimental PhysiologyNovember 1, 2024Michael J. McKenna, Xiaofei Gong, Aaron C. Petersen, Simon Sostaric, Craig A. Goodman, Andrew Garnham, Tai‐Juan Aw, Collene H. Steward, Kate T. Murphy, Kate A. Carey, et al.sample, 1 µg of RNA was transcribed into cDNA using the Promega AMV Reverse Transcription Kit (kit A3500; Promega, Madison, WI, USA), and the resulting cDNA was stored at −20°C for subsequent analysis. Real Time‐PCR

(43) Feeding Parenteral Nutrition in the Neonatal Period Programs Dyslipidemia in Adulthood in Yucatan Miniature PigsThe Journal of NutritionNovember 1, 2024Raniru S Randunu, Khaled Alawaini, Lee-Anne Huber, Edward W Randell, Janet A Brunton, Robert F BertoloComplementary DNA (was synthesized from the extracted RNA samples using reverse transcription (cat # A3500, Promega). Real-time quantitative polymerase chain reaction (qPCR) primers for microsomal triglyceride

(44) FUT6 Suppresses the Proliferation, Migration, Invasion, and Epithelial–Mesenchymal Transition of Esophageal Carcinoma Cells via the Epidermal Growth Factor Receptor/Extracellular Signal-Regulated Kinase Signaling PathwayTurkish Journal of GastroenterologyOctober 8, 2024Department of Surgery, Jinan University, Guangzhou, Guangdong Province, China, Jianle Lao, Department of Cardiothoracic Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi Province, China, Key Laboratory of Tumor Molecular Pathology of Baise , The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi Province, China, Yanmin Pang, Department of Hematology, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi Province, China, Hongming Chen, Department of Cardiothoracic Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi Province, China, Xiqiang Tang, Department of Cardiothoracic Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi Province, China, et al.from cells with Trizol reagent, RNA was converted into cDNA with a reverse transcription kit (cat. no. A3500; Promega, USA) according to the manufacturer’s instructions, followed by carrying out experiments with

(45) Viral etiology of febrile respiratory syndrome among patients in Liaoning Province, ChinaBMC Infectious DiseasesSeptember 27, 2024Baihong Sun, Yuzhu Qiu, Lulu Wang, Haibo Sun, Zhiqian Wang, Lingling Mao, Wei Wucat. No. 955134), Reverse Transcriptase Promega® Reverse Transcription System (Promega, USA, cat. No. A3500); Multiplex PCR Kit Seeplex® RV12 ACE Detection (Seegene, USA, cat. No. RV6C00Y); Agarose (Sigma, USA,

(46) A point mutation in VIG1 boosts development and chilling tolerance in riceNature CommunicationsSeptember 18, 2024Dunpin Xiong, Juan Wang, Ruci Wang, Yueming Wang, Yi Li, Ge Sun, Shanguo Yaofirst-strand cDNA was synthesized from 1 μg total RNA using the reverse transcription kit (Promega, No. A3500), according to the manufacturer’s instructions after digestion with RNase-free DNase I (Thermo Scientific,

(47) Digoxin and exercise effects on skeletal muscle Na+,K+‐ATPase isoform gene expression in healthy humansExp PhysiolSeptember 2, 2024Michael J. McKenna, Xiaofei Gong, Aaron C. Petersen, Simon Sostaric, Craig A. Goodman, Andrew Garnham, Tai‐Juan Aw, Collene H. Steward, Kate T. Murphy, Kate A. Carey, et al.sample, 1 µg of RNA was transcribed into cDNA using the Promega AMV Reverse Transcription Kit (kit A3500; Promega, Madison, WI, USA), and the resulting cDNA was stored at −20°C for subsequent analysis. Real Time‐PCR

(48) Control of lysogeny and antiphage defense by a prophage-encoded kinase-phosphatase moduleNature CommunicationsAugust 23, 2024Yunxue Guo, Kaihao Tang, Brandon Sit, Jiayu Gu, Ran Chen, Xinqi Shao, Shituan Lin, Zixian Huang, Zhaolong Nie, Jianzhong Lin, et al.RNA was used for reverse transcription reactions to synthesize cDNA with reverse transcription system A3500 (Promega, Madison, WI). Then, 50 ng of cDNA was used to conduct qRT-PCR with SYBR green reaction mix

(49) Region-specific protective effects of monomethyl fumarate in cerebellar and hippocampal organotypic slice cultures following oxygen-glucose deprivationPLOS ONEAugust 7, 2024Oliver Althaus, Nico ter Jung, Sarah Stahlke, Carsten Theiss, Jennifer Herzog-Niescery, Heike Vogelsang, Thomas Weber, Philipp Gude, Veronika Matschkeaccording to manufacturer’s protocol. cDNA synthesis was performed with a reverse transcription system (#A3500, Promega, USA). Following the manufacturer’s protocol, 1 μg total RNA and oligo(dT) primer were used.

(50) Skeletal dysmorphology and mineralization defects in Fgf20 KO miceFront Endocrinol (Lausanne)July 26, 2024Sylvie Dlugosova, Frantisek Spoutil, Carlos Eduardo Madureira Trufen, Betul Melike Ogan, Michaela Prochazkova, Olha Fedosieieva, Petr Nickl, Goretti Aranaz Novaliches, Radislav Sedlacek, Jan ProchazkaRNA samples (0.5 µg) were reverse-transcribed into cDNA using the Reverse Transcription System (PR-A3500, Promega, USA) according to the manufacturer’s instructions. RT-qPCR was carried out in a LightCycler

(51) Overexpression of macrophage migration inhibitory factor protects against pressure overload‐induced cardiac hypertrophy through regulating the miR‐29b‐3p/HBP1 axisPhysiological ReportsJune 26, 2024Liang Wen, Wei Chen, Cunjun Zhu, Jie Li, Juan Zhou, Minxia Zhang, Wenqiang Zhang, Qiang Xue(RM0051, AccuRef Scientific, China) and reverse‐transcribed into cDNA using HiFiscript cDNA synthesis kit (A3500, Promega, USA). Expression of lipid metabolism associated genes was analyzed by qPCR using SYBR Green

(52) Frameshift variants in C10orf71 cause dilated cardiomyopathy in human, mouse, and organoid modelsJournal of Clinical InvestigationJune 17, 2024Yang Li, Ke Ma, Zhujun Dong, Shijuan Gao, Jing Zhang, Shan Huang, Jie Yang, Guangming Fang, Yujie Li, Xiaowei Li, et al.protocol (Invitrogen, 15596018). RNA was reverse transcribed using a reverse transcription kit (Promega, A3500) and random hexamer primers. The RT reaction was set as follows: 25°C for 10 minutes; 42°C for 60 minutes;

(53) Frameshift variants in C10orf71 cause dilated cardiomyopathy in human, mouse, and organoid modelsJ Clin InvestJune 17, 2024Yang Li, Ke Ma, Zhujun Dong, Shijuan Gao, Jing Zhang, Shan Huang, Jie Yang, Guangming Fang, Yujie Li, Xiaowei Li, et al.protocol (Invitrogen, 15596018). RNA was reverse transcribed using a reverse transcription kit (Promega, A3500) and random hexamer primers. The RT reaction was set as follows: 25°C for 10 minutes; 42°C for 60 minutes;

(54) Primary oocytes with cellular senescence features are involved in ovarian aging in miceScientific ReportsJune 13, 2024Hao Yan, Edgar Andres Diaz Miranda, Shiying Jin, Faith Wilson, Kang An, Brooke Godbee, Xiaobin Zheng, Astrid Roshealy Brau-Rodríguez, Lei Leiwas carried out using 1 μg of total RNA and random primers (Reverse Transcription System, Promega, A3500). IQ™ SYBR® Green qPCR Supermix (Bio-Rad, 1708887) was employed for the real-time PCR assay. PCR reactions

(55) Dual role of GRHL3 in bladder carcinogenesis depending on histological subtypesMolecular oncologyJune 1, 2024Lammert FC, Pannhausen J, Noetzel E, Friedland F, Wirtz J, Herfs Y, Leypold S, Gan L, Weiskirchen R, Schnitzler T, et al.protocols (ANP). cDNA synthesis was accomplished with the reverse transcription system (Promega Kit A3500), using 1 μg of total RNA. 2.5 Quantitative real‐time reverse transcription PCR mRNA expression of

(56) Ginsenoside Rb1 induces hepatic stellate cell ferroptosis to alleviate liver fibrosis via the BECN1/SLC7A11 axisJournal of Pharmaceutical AnalysisMay 1, 2024Lifan Lin, Xinmiao Li, Yifei Li, Zhichao Lang, Yeping Li, Jianjian ZhengThermo Fisher Scientific Inc.). Complementary DNA (cDNA) was synthesized using a reverse transcription kit (A3500; Promega, Beijing, China). The SYBR Green Master Mix (TOROIVD, Shanghai, China) was employed to determine

(57) Exercise training affects calcium ion transport by downregulating the CACNA2D1 protein to reduce hypertension-induced myocardial injury in miceiScienceApril 19, 2024Shan Gao, Wei Yao, Rui Zhou, Zuowei PeiqPCR Master Mix (2X),Promega Technology,Cat# LS2062 Reverse Transcription System,Promega Technology,Cat# A3500 BCA kit,Shanghai -Beyotime Biotechnology,Cat# P6551 High Sensitivity DNA Kit,Agilent Technologies

(58) PHD finger protein 10 promotes cell proliferation by regulating CD44 transcription in gastric cancerHeliyonApril 15, 2024Fan Z, Jiang X, Yan W, Li J, Yan M, Liu B, Yu B.protocol. Then 1 μg RNA was reversely transcripted into 20 μL cDNA using the reverse transcription kit (A3500, Promega, USA). QRT-PCR analysis was performed using the ABI 7900HT system (Applied Biosystems, USA)

(59) Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicatorExperimental and Therapeutic MedicineMarch 26, 2024Yang Chen, Ren Li, Ziao Li, Biao Yang, Jianhang He, Jiayu Li, Peize Li, Zihan Zhou, Yongqiang Wu, Yuanli Zhao, et al.(Thermo Fisher Scientific, Inc.). The RNA was reverse transcribed using the reverse transcription kit (cat. no. A3500; Promega Corp.) according to the manufacturer's instructions, and qPCR assays were performed with SYBR

(60) Transcriptome sequencing and expression analysis in peanut reveal the potential mechanism response to Ralstonia solanacearum infectionBMC Plant BiolMarch 21, 2024Xiao Wang, Feiyan Qi, Ziqi Sun, Hongfei Liu, Yue Wu, Xiaohui Wu, Jing Xu, Hua Liu, Li Qin, Zhenyu Wang, et al.Beijing, China). First, RNA was reverse-transcribed to cDNA using the Reverse Transcription System A3500 (Promega, Madison, WI, USA). Next, the qRT-PCR was performed in triplicate for each sample using the

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