Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Identification and Phylogenetic Characterisation of Novel Adeno‐Associated Virus Capsids in Non‐Human Primate TissuesCell ProliferationSeptember 16, 2025Liyu Zhu, Kai Xu, Yali Ding, Kailun Liu, Jing Liu, Zongren Hou, Rui Niu, Ning Yang, Hualing Qin, Baoyang Hu, et al.to RNA purification (Invitrogen, 12183018A), followed by reverse transcription into cDNA (Promega, A3500). 2.3 AAV Genomic DNA Characterisation and Phylogenetic Reconstruction Nested PCR amplification was

(22) Arrestin-biased allosteric modulator of neurotensin receptor 1 alleviates acute and chronic painCellAugust 7, 2025Ran Guo, Ouyang Chen, Yang Zhou, Sangsu Bang, Sharat Chandra, Yize Li, Gang Chen, Rou-Gang Xie, Wei He, Jing Xu, et al.16894 Protease inhibitor cocktail,Millipore/Sigma,Cat# P8340 Reverse Transcription System,Promega,Cat # A3500 Coelenterazine 400a (hydrochloride),Cayman Chemical,Cat # 25738 Critical commercial assays,Critical

(23) Intrauterine growth-restricted (IUGR) female Yucatan miniature pig neonates fed parenteral nutrition exhibit early catch-up growth leading to obesity and ectopic fat deposition in adulthoodThe Journal of NutritionAugust 1, 2025Raniru S Randunu, Lee-Anne Huber, Janet A Brunton, Robert F Bertoloagarose gel. cDNA was produced from the extracted RNA samples using reverse transcription (catalog # A3500; Promega). Real-time qPCR primers (acetyl-CoA carboxylase (ACC-1), fatty acid synthase (FASN), sterol

(24) Identifying pyroptosis-related genes as novel therapeutic targets in diabetic foot ulcerationDiabetol Metab SyndrAugust 1, 2025Kang Liu, Lei Lei, Xin-Lei Yang, Xin-He Zhang(Qiagen, 74106). cDNA synthesis was carried out using 1 µg of RNA and an RT reaction mixture (Promega, A3500). qPCR was performed on an I-Cycler and IQ real-time PCR system (Bio-Rad) with SYBR Green I. Gene expression

(25) Differentiation to insulin-positive cells from human amnion epithelial cells using a pancreatic development mimicry protocolHistochem Cell BiolJuly 14, 2025Daniel Martínez-Rodríguez, Jonathan Salazar-Alonso, Axel Castro-Abrego, Daniela Ávila-González, Omar Martínez-Alarcón, Anayansi Molina-Hernández, Alejandro Martínez-Juárez, Héctor Salvador Godoy-Morales, Diana Elizabeth Lara-Barragán, Wendy Portillo, et al.was synthesized using the reverse transcription system (Promega Corporation, Madison, Wisconsin, USA; A3500). A total of 800 ng of RNA was mixed with 5 mM MgCl2, 1× reverse transcription buffer (10 mM Tris-HCl,

(26) Integrated Computational and Functional Screening Identifies G9a Inhibitors for SETD2-mutant LeukemiaGenomics, Proteomics & BioinformaticsMay 30, 2025Ya Zhang, Mengfang Xia, Zhenyi Yi, Pinpin Sui, Xudong He, Liping Wang, Qiyi Chen, Hong-Hu Zhu, Gang Huang, Qian-Fei Wang(1) mRNAs and lncRNAs were reverse transcribed using the Reverse Transcription System (Catalog No. A3500, Promega); (2) miRNAs were reverse transcribed using stem-loop-specific primers to ensure accurate transcription

(27) LncRNA SNHG7/miR-181b-5p/TLR4 Activates Inflammation And Promotes Pyroptosis Through NF-κB Signaling in Diabetic NephropathyInflammationMay 15, 2025Min Zhang, Sheng-jiang Xue, Feng Yang, Meng Xiao, Yong-Bo Tang, Ying Wuµg of the extracted RNA was reverse-transcribed into cDNA using a reverse transcription kit (Cat. #A3500, Promega, Madison, USA, US). The resulting cDNA samples were then analyzed using the SYBR Two-Step RT-qPCR

(28) Integrated metabolomics and network pharmacology analysis to reveal the mechanisms of Wenshenyang decoction in the treatment of chronic kidney diseaseFront PharmacolApril 30, 2025Ge Jin, Zongjiang ZhaoG4001). CCK-8 kit (Biorigin, BN15201). TriZol (LABLEAD, R1000). cDNA reverse transcription kit (Promega, A3500). Eastep® qPCR Master Mix (Promega, LS 2062). 2.6.2 Methods 2.6.2.1 Cell culture HK-2 cells were

(29) Mechanisms of Total Glucosides of Paeony in Alleviating Methotrexate-Induced Liver InjuryDrug Design, Development and TherapyApril 29, 2025Guang-Yao Chen, Xiang-Yu Ji, Ying Li, Si-Si Zheng, Qi Jin, Qing-Wen TaoChina), HiPure Total RNA Mini Kit (R4011, Magen Biotechnology, China), Reverse Transcription System (A3500, Promega, USA), SYBR ® Green Realtime PCR Master Mix (QPK-201, TOYOBO, Japan), BCA Protein Assay Kit

(30) Mechanisms of Total Glucosides of Paeony in Alleviating Methotrexate-Induced Liver InjuryDrug Des Devel TherApril 29, 2025Guang-Yao Chen, Xiang-Yu Ji, Ying Li, Si-Si Zheng, Qi Jin, Qing-Wen TaoChina), HiPure Total RNA Mini Kit (R4011, Magen Biotechnology, China), Reverse Transcription System (A3500, Promega, USA), SYBR ® Green Realtime PCR Master Mix (QPK-201, TOYOBO, Japan), BCA Protein Assay Kit

(31) Oncolytic virotherapy provides a potent therapy option for squamous bladder cancerSci RepApril 18, 2025Julia Pannhausen, Julia Wirtz, Klaus Mantwill, Per-Sonne Holm, Kristina Schwamborn, Danny D. Jonigk, Jürgen E. Gschwend, Michael Rose, Nadine T. Gaisa, Roman Nawrothaccording to manufacturer’s instructions. For cDNA synthesis the reverse transcription system kit (A3500, Promega) was used with 1 µg of total RNA and Thermal-Cycler C1000 Touch (Bio-Rad). Mastermix components

(32) Elevated levels of S100A8 and S100A9 exacerbate muscle mitochondrial fragmentation in sepsis-induced muscle atrophyCommunications BiologyFebruary 28, 2025Dongqin Huang, Yang Li, Yuqian Guo, Mengcao Weng, Hui Ye, Yan Zhang, Fei Lin, Kai Zhang, Xiangming FangThe obtained RNA was reverse transcribed into cDNA using the Reverse Transcription System (Promega, A3500) according to the manufacturer’s protocol. Power SYBR® Green RT-PCR reagent kit (Takara, Kyoto, Japan)

(33) A noncanonical intrinsic terminator in the HicAB toxin-antitoxin operon promotes the transmission of conjugative antibiotic resistance plasmidsNucleic Acids ResFebruary 27, 2025Jianzhong Lin, Songwei Ni, Baiyuan Li, Yunxue Guo, Xinyu Gao, Yabo Liu, Lingxian Yi, Pengxia Wang, Ran Chen, Jianyun Yao, et al.transcription process, 200 ng of total RNA was used to synthesize complementary DNA (cDNA) with the A3500 Reverse Transcription System (Promega, Madison, WI). Subsequently, 50 ng of the synthesized cDNA was

(34) Adaptive immune cells antagonize ILC2 homeostasis via SLAMF3 and SLAMF5Sci AdvJanuary 10, 2025Yuande Wang, Dan Li, Yehai Liu, Shasha Chen, Zhongjun Dongthe TRIzol Kit (Invitrogen) and reverse transcribed using the reverse transcription system (Promega, A3500). Quantitative polymerase chain reaction (PCR) was performed using SYBR Green–based detection. The expression

(35) The Intricate Mechanisms of Functional Foods Oyster Mushroom and Fenugreek on Type 2 Diabetic Animal ModelJournal of Diabetes ResearchDecember 9, 2024Arafat Hassan Razon, Md. Alauddin, Nisat Farzana, Sanaullah Mazumdar, Md. Ruhul Amin, Md. Mahedi Hassan Tusher, Md. Asrafuzzaman, Nahid Hasan, Mahfuzur Rahman, Muhammad Saiedullah, et al.(cDNA) by utilizing a high-capacity cDNA reverse transcriptase kit (Promega Corp. United States, Cat. A3500) [36]. In 10 μL reaction volume, 1 μg of mRNA was utilized. To observe the expression of GLUT-4, 3.0

(36) Prophylactic clemastine treatment improves influenza A virus-induced cognitive dysfunction in miceBrain, behavior, & immunity - healthDecember 1, 2024Tingling JD, Krauklis SA, Haak PL, Carr R, Louie AY, Johnson RW, Steelman AJ.lungs. Following isolation, RNA was converted into cDNA using the Reverse Transcription System (Promega, A3500) according to manufacturer's instructions in a C1000 Touch Thermal Cycler (Bio-Rad Laboratories). Gene expression

(37) T Lymphocyte-Macrophage Hybrid Membrane-Coated Biomimetic Nanoparticles Alleviate Myocarditis via Suppressing Pyroptosis by Targeting Gene SilencingInternational Journal of NanomedicineNovember 29, 2024Yulong Xiong, Zhenhao Zhang, Shangyu Liu, Lishui Shen, Lihui Zheng, Ligang Ding, Limin Liu, Lingmin Wu, Zhicheng Hu, Le Li, et al.Scientific). Reverse transcription was performed with the GoScriptTM Reverse Transcription System (A3500, Promega, USA). Realtime qPCR was carried out using GoTaq® qPCR and RT-qPCR Systems (A6002, Promega,

(38) T Lymphocyte-Macrophage Hybrid Membrane-Coated Biomimetic Nanoparticles Alleviate Myocarditis via Suppressing Pyroptosis by Targeting Gene SilencingInt J NanomedicineNovember 29, 2024Yulong Xiong, Zhenhao Zhang, Shangyu Liu, Lishui Shen, Lihui Zheng, Ligang Ding, Limin Liu, Lingmin Wu, Zhicheng Hu, Le Li, et al.Scientific). Reverse transcription was performed with the GoScriptTM Reverse Transcription System (A3500, Promega, USA). Realtime qPCR was carried out using GoTaq® qPCR and RT-qPCR Systems (A6002, Promega,

(39) Engrailed-2 (EN2) protein in cervical mucus: a novel biomarker for endometrial carcinomaClinical and Translational OncologyNovember 28, 2024Tong Wang, Ningyi Jia, Songkun Gao, Shengjie Liu, Ming Liu, Hong Zhangspectrophotometry. Reverse transcription (RT) was performed using the Reverse Transcription System (A3500, Promega, Madison, WI, USA) according to the manufacturer’s instructions. Real-time PCR was performed

(40) Localization of brain neuronal IL-1R1 reveals specific neural circuitries responsive to immune signalingJournal of neuroinflammationNovember 19, 2024Nemeth DP, Liu X, Monet MC, Niu H, Maxey G, Schrier MS, Smirnova MI, McGovern SJ, Herd A, DiSabato DJ, et al.Scientific). RNAs were reverse transcribed into cDNAs using a Reverse Transcription Kit (Promega, Cat# A3500). To detect the restored IL-1R1 mRNA, SYBR qPCR was performed using the following primers, which detect

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