Mouse cell lines are established from mouse tissue that originates from liver, neural segments, blood, tumorigenic cells, and fibroblasts. The purpose of choosing a particular type of tissue is for disease or protein assessment, or retroviral vector production and cell culture support. Embryonic neural cells are cultured from the hippocampus, pituitary, cortical, striatal and several additional derivatives of the central nervous system (CNS). Embryonic CNS neurospheres offered here are differentiated into neural and neural support cell lines. Engineered cell lines are available from cardiac, neural, and fibroblasts among others. These engineered lines are used to study DNA repair, cell cycle, and have been altered through transfection with viruses, irradiation, chemical treatment, or genetically alteration. Many of the mouse cell lines offered contain a knockout gene or a resistance to antibiotics. Wild-type cell lines are available for the comparison to the manipulated or tumorigenic cell lines. The diversity of mouse cell lines promoted here fit the users proposed research needs.