4T1 from ATCC

Product Specs
Item4T1
CompanyATCC
Price $125.00Supplier Page View Company Product Page
Catalog NumberCRL-2539
TypeCell Biology
SpeciesMus musculus
TissueMammary gland, Breast
Cell Line4T1
Validated Species ReactivityMouse (Ms)
Tissue (standardized)Mammary Gland
DiseaseMimics human breast cancer Stage IV
ApplicationsThe tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer. 4T1-induced tumors can be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell linesInternational Journal of HyperthermiaDecember 31, 2026Samir V. Jenkins, Hayden Carlton, Bree Scott, Shelbie D. Stahr, Whit Vinson, Gabriel Fletcher, Azemat Jamshidi-Parsian, Ping-Ching Hsu, Ruud P. M. Dings, David Huitink, et al.to inorganic nanoparticles. Methods Cell culture Mycoplasm-free murine breast cancer (4T1, ATCC CRL-2539), Lewis lung carcinoma (LLC, ATCC CRL-1642), and squamous cell carcinoma (SCCVII, Dept. of Radiology,

(2) PD-1 Blockade-Induced DKK1 Expression by CD8+ T Cells Promotes Blood-Brain Barrier PermeabilizationCancer DiscoveryMay 1, 2026Abhilash Deo, Sapir Levin, Chen Buxbaum, Madeleine Benguigui, Bar Manobla, Galit Saar, Noam Bosak, Eyal Bergmann, Ayelet Eran, Anat Grinfeld, et al.setting. Methods Cell Lines and Culture Conditions Murine EMT6 (cat. #CRL-2755; RRID:CVCL_1923), 4T1 (cat. #CRL-2539; RRID:CVCL_0125) mammary carcinoma, and LLC (cat. #CRL-1642; RRID:CVCL_4358) cell lines were purchased

(3) E-cadherin expression promotes tumor growth via KLRG1-dependent pathwaysJ ImmunolApril 24, 2026Jacob A Myers, Alexander R D Jordon, Samantha M Borys, KhanhLinh Dinh, Laurent BrossayPrime Therapeutics) and RMA-S were previously described.26 4T1 cells were purchased from ATCC (Cat# CRL-2539, RRID: CVCL_0125). The BWZ.36.KLRG1 reporter cell line was described previously.16 Plat-E (RRID: CVCL_B488)

(4) HE4 drives PD-L1 expression in myeloid cells via IFN-γR-JAK-STAT3 signaling to promote tumor immune evasionCell Reports MedicineApril 21, 2026Bo Zeng, Yingying Zhou, Heping Wang, Panyin Shu, Ting Pan, Fangfang Liu, Yangyang Li, Ming Yi, Ruirui He, Lingyun Feng, et al.Biotech,Cat# I451 Mouse: Raw264.7,ATCC,Cat# TIB-71 Human: THP-1,ATCC,Cat# TIB202 Mouse: 4T1,ATCC,Cat# CRL-2539 Mouse: MC38,ATCC,Cat# CRL-2640 Mouse: HGS-1,CancerTools. org,Cat# 160537 Bone marrow-derived macrophages,This

(5) ATP-Responsive Bimetallic Metal-Organic Frameworks Amplify Oxidative Stress in the Tumor Microenvironment for Synergistic Chemo-ImmunotherapyJ Funct BiomaterApril 19, 2026You Li, Wenxin Zhang, Zitao Xu, Shixin Ma, Yufei Xiong, Li Yu, Huiling Gao, Yang Shu, Teng Feifrom BD Biosciences (San Jose, CA, USA). Mouse 4T1 cells were obtained from ATCC (Manassas, VA, USA; #CRL-2539). Recombinant murine GM-CSF (#HY-P7069), IL-4 (#HY-P7080), FAM (#HY-W021817), WST-1 (#HY-136976), Calcein-AM

(6) AK5 suppresses breast cancer progression and modulates anti-PD-L1 efficacy via the miR-182-5p/PD-L1 axisiScienceApril 17, 2026Guang-Shun Sun, Yi-Han Li, Li Li, Li-Li Fan, Hui Yang, Qun Lu, Zhao Liu, Shui Wang, Jin-Juan Penglines,Experimental models: Cell lines ,, MDA-MB-231,ATCC,HTB-26 HCC1806,ATCC,CRL-2335 4T1,ATCC,CRL-2539 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(7) Promotion of breast cancer by the DNPH1 enzymeiScienceApril 17, 2026Sangphil Oh, Yuan Sui, Sook Shin, Ralf JanknechtExperimental models: cell lines,Experimental models: cell lines,Experimental models: cell lines ,, Mouse: 4T1,ATCC,CRL-2539 Human: MCF10A,ATCC,CRL-10317 Human: SKBR3,ATCC,HTB-30 Human: MDA-MB-453,ATCC,HTB-131 Human: MCF7,ATCC,HTB-22 Human:

(8) Focused Ultrasound-Triggered Burst Release and Enrichment of Engineered Bacteria for Tumor TherapyBiomaterials ResearchApril 16, 2026Jinhee Yoo, Yunhee Hwang, Mihyeon Park, Honghyeon Ha, Myeong Ryeol Choi, Sung In Lim, Byullee Park, Yong Joo Ahn, Won Jong Kim, Gyoo Yeol Jung, et al.transplantation The 4T1 breast cancer cells [American Type Culture Collection (ATCC), catalog no. CRL-2539] were cultured in RPMI 1640 (LM011-01, Welgene, Gyeongsangbuk-do, Republic of Korea) supplemented with

(9) Inflammation- and resolution-programmed myeloid circuits govern therapeutic resistance in epithelial and mesenchymal triple-negative breast cancerJournal of Clinical InvestigationApril 15, 2026Liqun Yu, Charlotte Rivas, Fengshuo Liu, Yichao Shen, Ling Wu, Zhan Xu, Yunfeng Ding, Xiaoxin Hao, Weijie Zhang, Hilda L. Chan, et al.HEPES (HyClone) and antibiotics. All cell lines were cultured in a 5% CO2 37°C incubator. 4T1 (catalog CRL-2539), E0771 (catalog CRL-3461), and EMT6 (catalog CRL-2755) were obtained from ATCC, AT3 (catalog SCC178)

(10) BSA-Based Nanoparticles for Dual Loading of Pazopanib and Enzalutamide: Formulation Optimization and In Vitro Evaluation in Breast Cancer CellsPharmaceuticsApril 13, 2026Gizem Ruya Topal, Kubra Kilic, Meral Sarper, Ozgur Esim, Ayhan Savaser, Yalcin Ozkancarcinoma cell line 4T1 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA; CRL-2539). All other chemicals used were of analytical grade. 2.2. Preparation of BSA-NPs BSA nanoparticles

(11) Photoswitchable Silver(I) Complex with Anticancer and Antimicrobial PotentialACS OmegaApril 7, 2026Aleksandra Kręcigłowa, Marta Stolarek-Sipior, Patrycja Jagielska, Kamil Kamiński, Magdalena Skóra, Marlena Gryl, Artur Sikorski, Janusz Rak, Maria Nowakowska, Krzysztof Szczubiałkamouse mammary gland epithelial cells (NMuMG, ATCC CRL-1636), murine mammary carcinoma cells (4T1, ATCC CRL-2539), human nonsmall cell lung cancer (A549, ATCC CCL-185), human prostate cancer (PC3, ATCC CRL-1435),

(12) Advanced CD276-Targeting Dual-Payload Antibody-Drug Conjugates for Cancer TherapyCancer Res CommunApril 1, 2026Zhuoxin Zhou, Davis Ballard, Jiashuai Zhang, Zhantao Du, Natsorn Watcharadulyarat, Tanvi Varadkar, Srijita Chowdhury, Sathvik R. Kambam, Phoebe Hu, Selena Khanal, et al.fetal bovine serum (FBS, v/v) and 1% penicillin–streptomycin (Pen-Strep, v/v). Mouse TNBC cell lines 4T1 (ATCC, cat. #CRL-2539, RRID:CVCL_0125) and 4T1-FLuc (ATCC, cat. #CRL-2539-LUC2, RRID:CVCL_5I85) were grown in RPMI 1640 medium

(13) Doxorubicin enhances adipogenesis in an FGF2-dependent manner and induces a tumour-promoting secretory phenotypeJournal of Bone OncologyApril 1, 2026Lauren M. Kreps, Edward Yakubovich, Huijun Zhao, Selam Yimer, Edward Yang, Juliet Cruz, Barbara Vanderhyden, Christina L. AddisonStemCell Technologies (Vancouver, BC). PC3 prostate tumour cells (ATCC CRL-1435), 4T1 breast tumour cells (CRL-2539) and K562 leukemia tumour cells (CCL-243) were obtained from the American Type Culture Collection (ATCC,

(14) Expanding the toolbox of bioorthogonal activation of photosensitizers for precise photodynamic therapy through transition metal-mediated deallylationMaterials Today BioApril 1, 2026Feijie Xu, Qianqian Wu, Lin He, Lin Yang, Pui-Chi Loconditions The HeLa human cervical cancer cells (ATCC, CCL-2), 4T1 murine mammary carcinoma cells (ATCC, CRL-2539), MCF-7 human breast cancer cells (ATCC, HTB-22), and NIH 3T3 murine embryonic fibroblast cells were

(15) Benzyl-Naphthoquinones as Selective Anticancer Agents for Oral Squamous Cell Carcinoma via Apoptosis InductionBiomedicinesMarch 26, 2026Antonio Mendonça Marconi-Nicolau, Rebeca Gripp de Sá, Caroline Reis Santiago Paschoal, Lethícia Andrade de Almeida, Gabriel Ouverney, Ana Caroline dos Santos-Diniz, Anamel Blaudt Meira, João Pedro da Costa Faria Brunhosa, Luiz Carlos da Silva Pinheiro, Paula Alvarez Abreu, et al.Manassas, VA, USA) (ATCC numbers: CRL-1624, CRL-1629, CRL-1628, HB-8065, HTB-38, CCL-247, CCL-2, CRL-6475, CRL-2539, respectively). Primary normal human gingival fibroblasts (HGFs) were obtained from ATCC (PCS-201-018).

(16) TRIM21-mediated degradation of HILPDA overcomes anti-PD-1 immunotherapy resistance in breast cancer by limiting PD-L1 palmitoylationOncogeneMarch 24, 2026Xi Wang, Guangyan Li, Jiaming Wu, Baiyang Fu, Haoyang Zhang, Yichi Chen, Jianjiao Wang, Jianguo Zhang, Xi ChenTo generate a PD-1–resistant 4T1 breast cancer cell line (4T1-R), parental 4T1 cells (4T1-P, ATCC® CRL-2539™) were orthotopically injected into the fourth mammary fat pad of 6–8-week-old female BALB/c mice at

(17) Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cellsCell ReportsMarch 24, 2026Kevin C. Corn, Shannon E. Martello, Vinay K. Menon, Lucy S. Britto, Kara M. Simmons, Youssef K. Mohamed, Yoanna I. Ivanova, Abtin A. Ghelmansaraei, Sara A. Weidenbach, Tian Zhu, et al.fibroblasts64,"Kuperwasser, Tufts University",PNAS.0401064101 4T1 murine triple negative breast cancer cells,ATCC,Cat#CRL-2539; RRID:CVCL_0125 HEK293T cells,ATCC,Cat#CRL-3216; RRID:CVCL_0063 ,, Experimental models: Organisms/strains,Experimental models

(18) Virus-based IFNγ gene delivery and photodynamic therapy cooperate to remodel the tumor microenvironment and suppress breast cancerMolecular Therapy OncologyMarch 19, 2026Ksenija Korotkaja, Zhanna Rudevica, Darija Lapina, Dace Skrastina, Juris Jansons, Olga Nilova, Karina Spunde, Anna Zajakinatreatment. Materials and methods Cell lines Murine mammary gland adenocarcinoma 4T1 cells (ATCC CRL-2539) were cultured in DMEM-GlutaMAX (Cat. No. 31966-021; Gibco, Life Technologies, Thermo Fischer Scientific,

(19) Characterization of HER2-Positive Murine Breast Cancer Models for Investigating HER2-Targeted Therapy and ImmunotherapyCancers (Basel)March 19, 2026Yun Lu, Benjamin P. Lee, Abbigael V. Eli, Shannon E. Lynch, Ar Rafi Md Faisal, Jonathan Moye, Anna G. SoraceLines Triple-negative breast cancer (TNBC) mouse mammary carcinoma cell lines 4T1 (ATCC, Manassas, VA, USA, #CRL-2539) and EO771 (ATCC, #CRL-3461) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Thermo Fisher Scientific

(20) Amphiphilic Chitosan-PEI Hybrid Nanocarrier Enhances Delivery Efficiency and Immunogenicity of PEDV mRNA VaccinesInt J NanomedicineMarch 17, 2026Shangen Xu, Haiwen Zhong, Linyun Li, Yao Chen, Chenxi Sun, Zhicheng Lao, Afei Wang, Tianyu Qian, Xiaohua Wang, Kai Zhaofrom Beyotime, China. HEK-293T (ATCC CRL-3216), Vero (ATCC CCL-81), L929 (ATCC CCL-1), and 4T1 (ATCC CRL-2539) cells were obtained from ATCC (Manassas, VA, USA). The dendritic cell line DC2.4 was obtained from

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4T1 from ATCC

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