Primary Cells, Cell Lines and Microorganisms

Primary Cells, Cell Lines and Microorganisms Research in the life sciences has advanced to encompass a wide breadth of biological focuses, from the small and microbial to the living human. Consequently, researchers are presented with ever more options of different cellular material with which to progress their work. Primary cells, taken directly from the tissue sources, include blood (such as red blood cells and PBMC), neurons, hepatocytes, endothelial cells, smooth muscle cells, and much more. Stable and proliferating cell lines are available, such as HEK, CHO, HeLa and Jurkat. For cell-based assays, there are extensive options of several stably-expressing cell lines, as well as ready-to-transfect viral delivery particles to investigate virtually any gene of interest. Stem cells and progenitors, ranging from the pluripotent embryonic to the tissue-specific can be provided freshly isolated, or already growing in culture. Microbial strains, including various competent cells have also been developed to suit your cloning needs.

Recommended Resources:

  • Isolation, Culture, and Preservation of Primary Cells

    Isolation, Culture, and Preservation of Primary Cells

    Primary cells, though sensitive to grow, are prized for their abilities to better mimic in vivo physiological metabolism and behavior than cell lines derived from tumors or immortalized cells. This article provides some expert tips regarding the isolation, culture, and preservation of primary cells.
  • Upstream Manufacturing for Cell and Cell-based Gene Therapies

    Upstream Manufacturing for Cell and Cell-based Gene Therapies

    Cell therapies like CAR-T still face limitations and challenges, sometimes due to the quality of the starting cells or difficulties during processing. In this article, we discuss what to consider during upstream manufacturing of cell and cell-based therapies, including during cell harvesting, preservation, and growth.
  • Documentary: The Importance of Cell Line Authentication

    Documentary: The Importance of Cell Line Authentication

    For over 50 years, there has been consistent consensus among scientists that misidentified and contaminated cell lines are a significant problem. This documentary discusses the concerns surrounding cell line identification and what can be done.