MC3T3-E1 Subclone 4 from ATCC

Product Specs
ItemMC3T3-E1 Subclone 4
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCRL-2593
ApplicationsThese cell lines are good models for studying in vitro osteoblast differentiation, particularly ECM signaling. They have behavior similar to primary calvarial osteoblasts
TypeCell Biology
SpeciesMus musculus
TissueCalvaria, Bone
Cell LineMC3T3-E1 Subclone 4
Validated Species ReactivityMouse (Ms)
Tissue (standardized)Bone
Cell Typepreosteoblast
Ageneonate

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) p21 Knockdown as a Therapeutic Strategy for Focal Cartilage Injury RepairFASEB JApril 30, 2026Leila Larijani, Aria Ahadzadeh Ardebili, Nini Ortiz Vales, Andreas T. Stürmer, Roman J. Krawetzin 2% O2 for 21 days with medium changes every 2–3 days. MC3T3‐E1 preosteoblasts (subclone 4, ATCC CRL‐2593) were maintained in α‐minimum essential medium (α‐MEM; Gibco) supplemented with 10% fetal bovine serum

(2) Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts DifferentiationBiofactorsApril 12, 2026Jesús Bocio-Núñez, Mª. José Montoya-García, Mª. Ángeles Vázquez-Gámez, Daniel Martín, Pedro Chacón, Miguel Ángel Rico, Miguel Ángel Colmenero, Alberto Yufera, Mercè GinerCulture and Electrical Stimulation Protocol The mouse preosteoblast cell line MC3T3‐E1 (subclone 4, ATCC CRL‐2593) was obtained from American Type Culture Collection (Manassas, VA, USA). Cells were cultured in complete

(3) Osteoinductive chemically crosslinked hydrogel enables hydroxyapatite formation, enhanced by release of dexamethasone, strontium, and zinc, and exhibits antimicrobial propertiesRSC AdvApril 8, 2026Benjamin Gambrill, Lirong Yang, Polina Prokopovichbetween frequencies at 24 °C. 2.9 Cell culture The MC3T3-E1 osteoblast precursor cell line (ATCC® CRL-2593™) was selected as a standard model to assess osteogenesis from C57BL/6 mouse calvaria, resulting in

(4) Multifunctional MoS₂-PMMA bone cement with enhanced strength and antibacterial activity to overcome limitations of conventional materials in orthopedic surgeryBMC Musculoskelet DisordApril 7, 2026Changsheng Gong, ShengBo Shi, ZiJing Zhang, ZeTian Zhao, Zuo Liu, Zhe Wang, Xiaobing YuMC3T3-E1 cell line, which serves as a model for murine calvarial osteoblasts, was obtained from ATCC (CRL-2593, Beijing, China). To maintain a normal physiological state, cells were cultured at a constant temperature

(5) Mechanical Reliability and Biocompatibility of Graphene-Modified Photocurable Resins for Additive Manufacturing: Implications for Potential Biomedical UseACS OmegaApril 7, 2026Sara Lopez de Armentia, Victor Manuel Villapún, Yolanda Ballesteros, Juan Carlos del Real, Lucy Arkinstall, Sophie Constance Cox, Nicholas Dunne, Eva Pazcollected and used on a preseeded mouse osteoblastic precursor cell line (MC3T3-E1 Subclone 1, ATCC CRL-2593, P26–28). For eluent collection, samples were cleaned by immersion and sonication in industrial methylated

(6) Full-laser-enabled clean hierarchical structuring and multifunctional synergy for high-performance in vivo 3D-printed implantsMater Today BioApril 3, 2026Qirui Zhang, Xinyue Zhang, Shanshan Liang, Jiaru Zhang, Qi Ma, Yiyang Wang, Xing Li, Fusong Yuan, Yingchun Guan, Huaming WangIn vitro cell evaluation 2.7.1 Cell culture The mouse calvarial preosteoblast cell line MC3T3-E1 (ATCC CRL-2593, RRID:CVCL_5440) and the human gingival fibroblast cell line HGF-1 (ATCC CRL-2014, RRID:CVCL_3710) were

(7) Targeting EFEMP1 enhances chondrogenesis and inhibits hypertrophic differentiation in a spontaneous osteoarthritis mouse modelJ Mol Med (Berl)March 18, 2026Yuhan Chang, Pei-Heng Jiang, Yung-Heng Hsu, Yu-Chih Lin, Chih-Chien Hu, Yi-Zhen Li, Steve Wen-Neng Ueng, Mei-Feng Chen5’-ACGUGACACGUUCGGAGAATT-3’) were used for gene knockdown experiments. The mouse preosteoblast cell line MC3T3-E1 (ATCC CRL-2593; American Type Culture Collection, Manassas, VA, USA) was cultured in alpha Eagle’s Minimum Essential

(8) Advanced models of lobular breast cancer metastasis capture clinical organ tropism, endocrine response, and bone remodelingbioRxivMarch 18, 2026Joseph L. Sottnik, Mary E. Buchanan, Maria J Contreras-Zárate, Trinh C Pham, Maggie Musick, Thu H. Truong, Diana M Cittelly, Julie H. Ostrander, Matthew J. SikoraHTB-21), UACC-3133 (CRL-2988), and ZR-75–30 (CRL-1504) were maintained as described [29]. MC3T3-E1 (ATCC CRL-2593) and Raw264.7 (ATCC TIB-71) were maintained as described [30, 31]. Cells were incubated at standard

(9) C/EBPβ dictates postmenopausal FSHβ transcription and blockade of AEP/C/EBPβ pathway alleviates osteoporosisBone ResMarch 17, 2026Zhongyun Xie, Jianming Liao, Jing Xiong, Zhenlei Zhao, Keqiang YeRAW 264.7 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA, catalog#: CRL-2593 and catalog#: TIB-71). The MC3T3-E1 cells were cultured in alpha-MEM with 10% FBS and 0.1% penicillin/streptomycin,

(10) A Multifunctional Therapeutic Platform: Ce/Zn/Sr-Doped Mesoporous Bioactive Glass Nanoparticles for Bone RepairInt J Mol SciMarch 13, 2026Nattakan Sae-Sue, Wen-Ta Su, Poommaree Namchaiw, Kamolchanok Ngamkham, Nattida Suwanakitti, Parichart Naruphontjirakulsample. 3.6. In Vitro Cytotoxicity MC3T3-E1 cells, from a mouse pre-osteoblast cell line (ATCC®, CRL-2593 ™), were routinely cultured in α-MEM medium supplemented with 10% fetal bovine serum (FBS) and 100 U/mL

(11) In Vitro Assessment of Osteogenic Modulation and Molecular Responses Induced by Contemporary Endodontic Sealers in MC3T3-E1 Pre-OsteoblastsDent J (Basel)March 11, 2026Yuka Miyamoto, Yuka Kato, Ryan Needle, Julie Yongsook Kim, Jin Koo Kim, Paul H. Krebsbach, Insoon Changmaterials. 2.2. Cell Culture and Viability Assay Murine MC3T3-E1 Subclone 4 pre-osteoblasts (ATCC CRL-2593™, ATCC, Manassas, VA, USA) were maintained in α-MEM supplemented with 10% fetal bovine serum and 1%

(12) Bone Morphogenetic Protein (BMP) 9 Outperforms BMP2 in Osteogenesis and Osseointegration: In Vitro and In VivoClin Implant Dent Relat ResMarch 10, 2026Hee-Seung Han, Dong Woo Lee, Yu-Bin Kim, Sanghui Seok, Jeong Yeon Park, Sungtae Kim, Jue Yeon Lee, Yoon Jeong Park, Chong Pyung Chung, Young-Dan ChoThe final drug substance was concentrated and lyophilized. Mouse pre‐osteoblast MC3T3‐E1 (subclone 4; CRL‐2593), murine macrophage RAW 264.7 (TIB‐71) cells (ATCC, Manassas, VA, USA), and human periodontal ligament

(13) Optimizing Titanium Osseointegration through Thermally Modified Co-Doped Monetite CoatingsACS Biomaterials Science & EngineeringMarch 9, 2026Gerson Santos de de Almeida, Maria Gabriela Jacheto Carra, Matheus Luquirini Penteado dos Santos, Julia Bucci, Luisa Camilo Suter, Diego Rafael Nespeque Corrêa, Pascale Chevalier, Margarida Juri Saeki, Diego Mantovani, Willian Fernando Zambuzziindependent scans per sample group. Osteoblasts Culture The MC3T3-E1 preosteoblast cell line (ATCC CRL-2593) was used between passages 15 and 25. Cells were routinely tested and confirmed to be free of mycoplasma

(14) Hexagonal-to-Monoclinic Phase-Modulated HAp Nanofibers for Enhanced Piezoelectric and Biocompatible PerformanceBiomoleculesMarch 4, 2026Karime Carrera-Gutiérrez, Estefania Venegas-Contreras, Miguel Márquez-Torres, Marco Antonio Ruiz-Esparza-Rodríguez, Yasmin Esqueda-Barrón, Roberto Gomez-Batres, Irene Leal-Berumen, Jorge Noé Díaz de León, Juan José Gervacio-Arciniega, Guillermo Herrera-Pérez, et al.and 21 days to allow potential leaching of ionic species. Mouse pre-osteoblast MC3T3-E1 cells (ATCC CRL-2593, passages 3–7) were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS; Gibco) and

(15) Biomedical titanium-tantalum alloys for orthopedic implant applications: From manufacturing to biological performanceBioact MaterMarch 2, 2026S.D. Luo, S. Paisansuthichol, N. Tran, M. Qian, T. SongTi∗,-,-,-,CC-7049,100,-,-,-,-,- Ti-20Ta,DED-LB/M,"Grid, 400–450 μm pore size",As-built,Mouse calvarial preosteoblasts (ATCC CRL-2593),0.008d,24,-,-,-,-,[121] DED-LB/M,"Grid, 400–450 μm pore size",As-built,Mouse calvarial preosteoblasts

(16) Residuals of Chemical Cleaning Agents Impair Peri-Implant Cell Viability: An in Vitro StudyACS Biomaterials Science & EngineeringFebruary 9, 2026Qiang Wang, Håvard Jostein Haugen, Dirk Linke, Ståle Petter Lyngstadaas, Qianli MaReykjavik, Iceland) to obtain graded concentrations as shown in Table . 2.2 Cell Culture MC3T3-E1 cells (CRL-2593, ATCC, Manassas, VA, USA) were cultured in Minimum Essential Medium Alpha (α-MEM, A1049001, Gibco, USA)

(17) Material-dependent effects of injectable platelet rich-fibrin on growth factor release, inflammation, and osteoclast activity: an in vitro studyBMC Oral HealthJanuary 16, 2026Lauma Ievina, Elena Della Bella, Lana Micko, Ilze Salma, Alexander Sieberath, Arita Dubnikaeach collection, 1.5 mL of fresh α-MEM was added to each well. Osteoblast expansion MC3T3-E1 (ATCC CRL-2593, USA) passage 6 (mycoplasma free (tested with Rapid Mycoplasma Detection Kit (MORV0011), Assay Genie,

(18) Comprehensive Toxicological Evaluation of 3D-Printed Hydroxyapatite (3DPHA) for Bone Grafting ApplicationsInternational Journal of Molecular SciencesJanuary 8, 2026Faungchat Thammarakcharoen, Autcharaporn Srion, Waraporn Suvannapruk, Watchara Chokevivat, Wiroj Limtrakarn, Jintamai Suwanprateeb570blank 4.6. In Vitro MC3T3-E1 Cell Proliferation The MC3T3-E1 preosteoblast cells (Subclone 4, ATCC CRL-2593, ATCC, Manassas, VA, USA) were utilized to evaluate cell compatibility and proliferation. Samples were

(19) Surface Engineering of Non-Equiatomic TiZrNbTaMo HEA by MAO Treatment in a Cu-Rich Electrolyte for Biomedical ApplicationsMaterials (Basel)January 3, 2026Samuel P. Bonetti, Jhuliene E. M. Torrento, Carlos R. Grandini, Tiago dos S. P. de de Sousa, Gerson S. de de Almeida, Willian F. Zambuzzi, Diego R. N. Correacrystal violet (CV) colorimetric assays, where MC3T3-E1 mouse pre-osteoblastic cells (subclone 4; ATCC CRL-2593) were cultured in α-MEM supplemented with 10% fetal bovine serum (FBS) at 37 °C and 5% CO2. Sub-confluent

(20) Understanding of the different roles of Noggin in the Noggin-BMP-2 and Noggin-BMP-9 dimer complexes at the molecular levelScientific ReportsJanuary 2, 2026Suhyun Park, Dong Woo Lee, Min-Ho Park, Jue Yeon Lee, Dakshinamurthy Sivakumar, Sathishkumar Mudedla, Yeon Gyo Seo, Sangwook Wu, Yoon Jeong Park, Chong Pyung Chungpre-osteoblastic cell line MC3T3-E1 was purchased from the American Type Culture Collection (ATCC, CRL-2593, Manassas, VA, USA). Cells were maintained in α-MEM (Welgene, LM008-01) supplemented with 10% fetal

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MC3T3-E1 Subclone 4 from ATCC

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MC3T3-E1 Subclone 4 from ATCC

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