Fig 1: Relative mRNA expression of melanoma genes GP100 and TRP-2 in different cell lines and normal tissues.To compare the expression of GP100 and TRP-2 in melanoma cells to that in other cell types, total RNA was extracted from three different sublines of B16.F10 melanoma cells, including a variant obtained directly from ATCC, from the amelanotic melanoma cell line B78-D14, and from the lymphoma cell line EL-4 (to serve as negative control). Using Q-PCR, gene expression was compared to that in three different tissues from normal C57BL/6 mice: liver, lungs and lymph nodes (LNs). Melanoma gene expression was quantified relative to the housekeeping gene GAPDH. The relative mRNA expression of B16.F10gp cells was used as calibrator and set to 1.