3T3 MEFs WT from ATCC

Product Specs
Item3T3 MEFs WT
CompanyATCC
Price $95.00Supplier Page View Company Product Page
Catalog NumberCRL-2752
TypeCell Biology
SpeciesMus musculus
TissueEmbryo
Cell Line3T3 MEFs WT
Validated Species ReactivityMouse (Ms)
Cell Type (standardized)Fibroblasts
Tissue (standardized)Embryo
Cell Typefibroblast
Age13.5 days gestation
ApplicationsThese cell lines are useful in studying the role of Caveolin-1 in a variety of signaling and membrane trafficking events

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) KAT6A chimeras form a self-reinforcing epigenetic module with NURF and MLL/COMPASS to sustain AMLGenome BiolAugust 19, 2025Junhui Lv, Zhinang Yin, Conghui Li, Honglin Wen, Jian Ni, Peiyuan Yang, Zemin Song, Ying Xiang, Honghong Wang, Rui Lu, et al.blood cancers. Methods Cell culture and DNA construction HEK293T (ATCC CRL-3216) and Mouse Embryonic Fibroblast (MEF; ATCC CRL-2752) cells were cultured in high-glucose DMEM (Life Technologies) supplemented with 10% FBS (LONSERA S711-001),

(2) Positive regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in miceNature CommunicationsOctober 10, 2023Tomas Venit, Oscar Sapkota, Wael Said Abdrabou, Palanikumar Loganathan, Renu Pasricha, Syed Raza Mahmood, Nadine Hosny El Said, Shimaa Sherif, Sneha Thomas, Salah Abdelrazig, et al.Knock-Out (NM1 KO) cell lines were derived from wild-type mouse embryonic fibroblasts (MEFs) (ATCC® CRL-2752) (NM1 WT) using the CRISPR/Cas9 system23. For the NM1 rescue experiment, we transduced NM1 KO MEFs by

(3) Lamin C is required to establish genome organization after mitosisGenome BiologyDecember 1, 2021Xianrong Wong, Victoria E. Hoskins, Ashley J. Melendez-Perez, Jennifer C. Harr, Molly Gordon, Karen L. Reddyexperiments, longer term-culture C57BL/6 MEFs were purchased from ATCC (American Tissue Culture Collection, CRL-2752) and cultured according to their established protocols, in medium containing DMEM High, 10% FBS, penicillin/streptomycin

(4) Mapping the micro-proteome of the nuclear lamina and lamina-associated domainsLife Science AllianceMay 1, 2021Xianrong Wong, Jevon A Cutler, Victoria E Hoskins, Molly Gordon, Anil K Madugundu, Akhilesh Pandey, Karen L Reddyrestriction sites. Cell line generation, reagents, and antibodies MEFs were purchased from ATCC (CRL-2752) and cultured according to their establish protocols. These MEFs were transduced with lentiviral particles

(5) Endophilin-A2-dependent tubular endocytosis promotes plasma membrane repair and parasite invasionJournal of cell scienceDecember 1, 2020Corrotte M, Cerasoli M, Maeda FY, Andrews NW.endosomes associated with PM repair and T. cruzi invasion. MATERIALS AND METHODS Cell culture WT (ATCC, CRL 2752) and Cav1 KO (ATCC, CRL 2753) 3T3 MEFs were purchased from the American Type Culture Collection and

(6) Nuclear myosin 1 activates p21 gene transcription in response to DNA damage through a chromatin-based mechanismCommunications BiologyDecember 1, 2020Tomas Venit, Khairunnisa Semesta, Sannia Farrukh, Martin Endara-Coll, Robert Havalda, Pavel Hozak, Piergiorgio Percipallederived from 13.5 days NM1 WT and KO embryos46. In other experiments were used immortalized MEFs (ATCC® CRL-2752) or cell lines derived from these immortalized cells. The antibodies against H3K9me3 (ab8898), H3K27ac

(7) Metformin is a novel suppressor for transforming growth factor (TGF)-β1Scientific reportsJune 28, 2016Xiao H, Zhang J, Xu Z, Feng Y, Zhang M, Liu J, Chen R, Shen J, Wu J, Lu Z, et al.were performed as previously described19. Briefly, 3T3 cells (American Type Culture Collection, ATCC® CRL-2752™) or HCT 116 cells (American Type Culture Collection, ATCC® CRL-247™) were seeded onto a 24-well-plate

(8) Cell resistant zwitterionic polyelectrolyte coating promotes bacterial attachment: an adhesion contradictionBiomaterials ScienceFebruary 13, 2016Jessica S. Martinez, Kristopher D. Kelly, Yara E. Ghoussoub, Jose D. Delgado, Thomas C. S. Keller III, Joseph B. SchlenoffRat aortic smooth muscle A7r5 cells and mouse fibroblast 3T3 cells (originally ATCC CRL 1444 and ATCC CRL 2752 respectively, both maintained through numerous passages and stored frozen in the lab over several years)

(9) Tuning the properties of pH responsive nanoparticles to control cellular interactions in vitro and ex vivoPolymer ChemistryJanuary 1, 2016S. K. Mann, A. Dufour, J. J. Glass, R. De Rose, S. J. Kent, G. K. Such, A. P. R. Johnstonpurchased from Lumiprobe. Cell lines 3T3 mouse embryonic fibroblast wild type cells (3T3 MEFs WT, ATCC CRL-2752) and human C1R cells (ATCC CRL-1993) were cultured in DMEM medium supplemented with 10% fetal bovine

(10) Human Metapneumovirus Is Capable of Entering Cells by Fusion with Endosomal MembranesPLOS PathogensDecember 2, 2015Reagan G. Cox, Bernardo A. Mainou, Monika Johnson, Andrew K. Hastings, Jennifer E. Schuster, Terence S. Dermody, John V. Williamsmanufacturer (293 Freestyle expression system; Invitrogen). BHK-21 (ATCC CCL-10), caveolin-1 wt (ATCC CRL-2752) and Cav-1-/- (ATCC CRL-2753) MEFs were maintained in DMEM medium containing 10% FBS. Viruses HMPV

(11) Type II Secretion-Dependent Degradative and Cytotoxic Activities Mediated by Stenotrophomonas maltophilia Serine Proteases StmPr1 and StmPr2Infection and immunityOctober 1, 2015DuMont AL, Karaba SM, Cianciotto NP.conditions suggested by the ATCC. The human HeLa cell line (ATCC CCL-2) and the murine 3T3 cell line (ATCC CRL-2752) were routinely cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Corning) containing 10% FBS.

(12) Directed targeting of chromatin to the nuclear lamina is mediated by chromatin state and A-type laminsThe Journal of cell biologyJanuary 5, 2015Harr JC, Luperchio TR, Wong X, Cohen E, Wheelan SJ, Reddy KL.(RP23-349M24) and Bcl11a (RP23-30C14 and RP23-153C18) loci into the genome of NIH3T3-derived C57BL/6 FB (ATCC CRL-2752; Figs. S1 B and S2, A and B). As controls, a BAC from the distal, but internal, portion of the Igh locus

(13) Nanoscale engineering of low-fouling surfaces through polydopamine immobilisation of zwitterionic peptidesSoft MatterApril 21, 2014Jiwei Cui, Yi Ju, Kang Liang, Hirotaka Ejima, Samuel Lörcher, Katelyn T. Gause, Joseph J. Richardson, Frank CarusoJ software. Mammalian Cell Attachment on Substrates NIH 3T3 mouse embryonic fibroblast cells (ATCC CRL-2752) were maintained at 37 °C with 5% CO2 in Dulbecco’s Modified Eagles’ Medium (DMEM) containing 10% (v/v)

(14) Invasion of epithelial cells by Campylobacter jejuni is independent of caveolaeCell communication and signaling : CCSDecember 23, 2013Konkel ME, Samuelson DR, Eucker TP, Shelden EA, O'Loughlin JL.10% CO2, 10% H2, 75% N2). Tissue culture HeLa (ATCC® CCL2™), Caco-2 (ATCC® HTB-37™), 3T3 MEF WT (ATCC® CRL-2752™), and 3T3 MEF KO (ATCC® CRL-2753™) cells were purchased from the American Type Culture Collection (Manassas,

(15) Caveolin-1-Enhanced Motility and Focal Adhesion Turnover Require Tyrosine-14 but Not Accumulation to the Rear in Metastatic Cancer CellsPLoS ONEApril 10, 2012Hery Urra, Vicente A. Torres, Rina J. Ortiz, Lorena Lobos, María I. Díaz, Natalia Díaz, Steffen Härtel, Lisette Leyton, Andrew F. G. Questin DMEM-F12 medium containing 10% FBS and antibiotics. Mouse embryonic fibroblasts MEF-3T3 (ATCC, #CRL-2752) were maintained in DMEM medium supplemented with 10% FBS and antibiotics. Metastatic murine melanoma

(16) A two-way communication between microglial cells and angiogenic sprouts regulates angiogenesis in aortic ring culturesPloS oneJanuary 10, 2011Rymo SF, Gerhardt H, Wolfhagen Sand F, Lang R, Uv A, Betsholtz C.medium changed every 2–3 days. MEF cells: Wildtype MEF cells were purchased from ATCC (catalog no. CRL-2752). The MEF cells were derived from embryonic fibroblast of mouse musculus with a 129Sv, C57Bl6 background.

(17) Focal-adhesion targeting links caveolin-1 to a Rac1-degradation pathwayJournal of cell scienceJune 1, 2010Nethe M, Anthony EC, Fernandez-Borja M, Dee R, Geerts D, Hensbergen PJ, Deelder AM, Schmidt G, Hordijk PL.in EGM2 medium (Lonza) prepared according to the manufacturer’s instructions. Cav1–/– knockout (ATCC-CRL-2752) and parental wild-type (ATCC-CRL-2753) MEFs were obtained from ATCC and cultured in DMEM containing

(18) The utilization of pathogen-like cellular trafficking by single chain block copolymerBiomaterialsMarch 1, 2010Sahay G, Gautam V, Luxenhofer R, Kabanov AV.the caveolin-1 gene Cav-1 -/-, ATCC CRL-2753) and 3T3 MEFs WT (wild type, Cav-1 +/+) cell line (ATCC CRL-2752) were maintained in DMEM, containing 10% heat inactivated FBS and 0.01% penicillin/streptomycin. Primary

(19) The exploitation of differential endocytic pathways in normal and tumor cells in the selective targeting of nanoparticulate chemotherapeutic agentsBiomaterialsFebruary 1, 2010Gaurav Sahay, Jong Oh Kim, Alexander V. Kabanov, Tatiana K. Bronichthe caveolin-1 gene Cav-1 −/−, ATCC CRL-2753) and 3T3 MEFs WT (wild type, Cav-1 +/+) cell line (ATCC CRL-2752) were maintained in DMEM, containing 10% heat inactivated FBS and 0.01% penicillin/streptomycin. Primary

(20) Preparation of biologically active subcellular fractions using the Balch homogenizerAnalytical BiochemistryNovember 1, 2009Christopher L. German, Charles L. HoweMethods Cell Lines and Reagents 3T3 mouse embryonic fibroblast (MEF) WT cells acquired from ATCC (CRL-2752) were grown in DMEM with 10% FBS and 40 μg/ml penicillin-streptomycin at 37° C in a 5% CO2 environment.

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3T3 MEFs WT from ATCC

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