Anti-CXCR4 antibodies are essential reagents in flow cytometry for detecting the target protein, C-X-C motif chemokine receptor 4. CXCR4 acts as a receptor for the chemokine CXCL12, leading to increased intracellular calcium levels and enhanced MAPK1/MAPK3 activation. CXCR4 also serves as a coreceptor for HIV-1 and a primary receptor for some HIV-2 isolates. Mainly a cell membrane protein, it can also be found in cell junctions, endosomes, and lysosomes. CXCR4 has been used as a phenotypic marker for myeloid-derived suppressor cells, NK T cells, granulocytes, basophils, monocytes, B cells, NK cells, and neutrophils. Examine the supplier's provided validation data when comparing CXCR4 antibodies for flow cytometry. Also look for application-specific recommendations, such as dilutions, protocols, sample types, and other helpful usage notes.