Anti-TLR4 antibodies are essential reagents in flow cytometry for detecting the target protein, toll like receptor 4. TLR4 acts as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. TLR4 is expressed in myeloid lineage immune cells, including monocytes, macrophages, dendritic cells, neutrophils, and eosinophils. Examine the supplier's provided validation data when comparing TLR4 antibodies for flow cytometry. Also look for application-specific recommendations, such as dilutions, protocols, sample types, and other helpful usage notes.