Fig 2: Identification of Let-7i mRNA targets. (A) Sequence alignment of Let-7i and its conserved target site in the TLR4 3'-UTR. (B) Luciferase activity, measured in Ishikawa cells with a dual-luciferase reporter assay. **P< 0.01.

Fig 3: Protective effect of extract of Lonicera japonica on PM2.5-induced protein expression of Western blot images (a). Protein expression levels of TLR-4 (b), MyD88 (c), p-JNK (d), p-NF-?B (e), p-NF-?B (f), COX-2 (g), iNOS (h), TNF-a (i), and IL-1ß (j). Results shown are mean ± SD (n = 3). Data were statistically represented at p < 0.05, and different lowercase letters indicate statistical significance.

Fig 4: The effect of microRNA Let-7i in the pathway of inflammatory regulatory. (A) The CD4+ T cells of patients with AS preprocessed with LPS were infected by Let-7i overexpression/interference Lenti-virus. The mRNA level of TLR4, NF-?B, and IFN-? were detected by qRT-PCR. (B and C) The protein expression level of inflammation factors was detected by WB. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

Fig 5: Let-7i and inflammation responses in PBMCs of patients with AS. (A) The mRNA level of Let-7i, TLR4, NF-?B, and IFN-? of patients with AS was detected by qRT-PCR. (B–D) Relative protein expression of inflammation factors of patients with AS was detected by WB. Data are represented as mean ± SD (n = 10), ***P<0.001, ****P<0.0001.