Anti-TLR4 antibody [76B357.1] ab22048 from Abcam

Product Specs
ItemAnti-TLR4 antibody [76B357.1]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab22048
Antibody TypePrimary
Clone76B357.1
HostMouse, Nude mouse
ClonalityMonoclonal
Quantity100 UG
IsotypeIgG2b
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Conjugate/TagUnconjugated
Concentration0.5 mg/ml
Research AreaImmuno-oncology
Gene NameTlr4
ReactivityHuman, Rat
TargetTLR4

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) SARS-CoV-2 activates the TLR4/MyD88 pathway in human macrophages: A possible correlation with strong pro-inflammatory responses in severe COVID-19HeliyonNovember 17, 2023Sabina Sahanic, Richard Hilbe, Christina Dünser, Piotr Tymoszuk, Judith Löffler-Ragg, Dietmar Rieder, Zlatko Trajanoski, Anne Krogsdam, Egon Demetz, Maria Yurchenko, et al.secondary antibody (1:500, Thermo Fisher, A11008). A monoclonal mouse anti-TLR4 antibody (1:100, Abcam ab22048) was incubated at 37 °C for 30 min, followed by 3 washing steps and incubation with the secondary antibody

(2) Pretreatment with geniposide mitigates myocardial ischemia/reperfusion injury by modulating inflammatory response through TLR4/NF-κB pathwayEuropean Journal of HistochemistrySeptember 8, 2023Yanmei Yao, Leqing Lin, Wenxue Tang, Yueliang Shen, Fayu Chen, Ning Li, Baiyong Wangalbumin. The sections were then incubated overnight with a primary antibody solution containing TLR4 (ab22048, Abcam, Cambridge, MA, USA) at 4°C. As negative controls, some sections were processed in the same way

(3) FTO-mediated m6A modification alleviates autoimmune uveitis by regulating microglia phenotypes via the GPC4/TLR4/NF-κB signaling axisGenes & diseasesSeptember 1, 2023He S, Li W, Wang G, Wang X, Fan W, Zhang Z, Li N, Hou S.NF-κB p65 (ab32536, Abcam, 1:1,000), NF-κB p-p65 S536 (3033, Cell Signaling Technology, 1:1,000), TLR4 (ab22048, Abcam, 1:1,000), MYD88 (ab133739, Abcam, 1:1,000), CD14 (221678, Abcam, 1:1,000), CXCL10 (14969, Cell Signaling Technology

(4) Dioscin Ameliorates Experimental Autoimmune Thyroiditis via the mTOR and TLR4/NF-κB SignalingDrug Des Devel TherAugust 2, 2023Chengfei Zhang, Qiue Zhang, Lingling Qin, Zhiyi Yan, Lili Wu, Tonghua LiuCybernetics system was used to detect absorbance and determine integrated option density (IOD)/area of TLR4 (ab22048, Abcam, Cambridge, MA, USA), NF-κB p65 (ab16502, Abcam), or mTOR (ab32028, Abcam), and to perform statistical

(5) Circulatory HMGB1 is an early predictive and prognostic biomarker of ARDS and mortality in a swine model of polytraumaFrontiers in ImmunologyJuly 14, 2023Matthew D. Young, Tomas S. Cancio, Catherine R. Thorpe, Robert P. Willis, John K. Snook, Bryan S. Jordan, Samandra T. Demons, Jose Salinas, Zhangsheng Yang(PBST) (Triton X-100, 0.1%), Abcam, Cambridge, MA] and mouse monoclonal to TLR4 [IgG2b, monoclonal, cat#ab22048, diluted in 1% goat serum with PBST (Triton X-100, 0.01%), Abcam, Cambridge, MA]. Following extensive

(6) Sodium Propionate Relieves LPS-Induced Inflammation by Suppressing the NF-ĸB and MAPK Signaling Pathways in Rumen Epithelial Cells of Holstein CowsToxins (Basel)July 3, 2023Chenxu Zhao, Fanxuan Yi, Bo Wei, Panpan Tan, Yan Huang, Fangyuan Zeng, Yazhou Wang, Chuang Xu, Jianguo Wangthen incubated at 4 °C overnight with multiple primary antibodies, including TLR4, MyD88, P-NF-κB p65 (ab22048, ab2068, ab86299, Abcam, Cambridge, UK), NF-κB p65 (AF5006, Affinity Biosciences, Jiangsu, China), P-IκBα,

(7) MiR-137 promotes TLR4/NF-κB pathway activity through targeting KDM4A, inhibits osteogenic differentiation of human bone marrow mesenchymal stem cells and aggravates osteoporosisJournal of Orthopaedic Surgery and ResearchJune 21, 2023Ying-feng Yu, Pei-quan Yao, Zhi-kun Wang, Wen-wei Xieovernight with the primary antibodies [Runx2, ab76956, 1:1000; osteocalcin (OCN), ab93876, 1:1000; TLR4, ab22048, 1:1000; P65, ab32536, 1:1000; p-P65, ab76302, 1:1000; Abcam, Cambridge, UK] that were used for protein

(8) Soluble CD4 effectively prevents excessive TLR activation of resident macrophages in the onset of sepsisSignal transduction and targeted therapyJune 19, 2023Zhang SY, Xu QP, Shi LN, Li SW, Wang WH, Wang QQ, Lu LX, Xiao H, Wang JH, Li FY, et al.(ab131541), phospho-SHP1 at Y536 (ab51171), anti-MHCII (ab180779), anti-MHCII (ab25681), anti-TLR4 (ab22048), anti-SHP2 (ab131541), anti-P-SHP2 (ab62322) mouse IgG isotype control (ab172730), rabbit IgG isotype

(9) Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axisCurrent Research in Food ScienceJune 15, 2023Qian He, Li-Li Zhang, Deming Li, Jiangxue Wu, Ya-Xin Guo, Jingbo Fan, Qingyang Wu, Hai-Peng Wang, Zhongxiao Wan, Jia-Ying Xu, et al.ab263899), IL-18(Abcam, ab243091), IL-1β (Abcam, ab254360), caspase-1 (Abcam, ab207802), TLR4 (Abcam, ab22048), myeloid differentiation factor 88 (MyD88; Abcam, ab133739), NF-κB p65 (Abcam, ab32536), GAPDH (ABclonal,

(10) Engeletin alleviates cerebral ischemia reperfusion‐induced neuroinflammation via the HMGB1/TLR4/NF‐κB networkJournal of Cellular and Molecular MedicineJune 1, 2023Yangyang Xu, Jie Zhang, Fei Gao, Wenna Cheng, Ye Zhang, Chuanmei Wei, Shuping Zhang, Xinfu Gaoedaravone group (6 mg/kg) and engeletin groups (15, 30 and 60 mg/kg). Antibodies (HMGB1: ab79823, TLR4: ab22048, Bcl‐2: ab32124, NF‐κB p65: ab16502, Bax: ab182733 and Cleaved Caspase‐3: ab214430) were acquired via

(11) Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4 /NF-κB pathwayEuropean Journal of HistochemistryMarch 20, 2023Qian Hu, Ran Tao, Xiaoyun Hu, Haibo Wu, Jianjun Xuexamine TLR4 expression and distribution, samples were incubated with primary antibody against TLR4 (ab22048, 1/100; Abcam) overnight at 4ºC, and then with secondary antibody (rabbit anti-rat IgG H&L, ab6734,

(12) Protective effects of cordycepin pretreatment against liver ischemia/reperfusion injury in miceImmunity, Inflammation and DiseaseMarch 14, 2023Yunxia Liu, Mingwei Sheng, Lili Jia, Min Zhu, Wenli Yup65 (diluted at 1:1000, ab16502), p‐p65 (diluted at 1:1000, ab86299), and GAPDH (diluted at 1:2000, ab22048) were purchased from Abcam. The primary antibodies of Bax (diluted at 1:1000, sc‐7480) and Bcl‐2 (diluted

(13) Dietary Supplement, Containing the Dry Extract of Curcumin, Emblica and Cassia, Counteracts Intestinal Inflammation and Enteric Dysmotility Associated with ObesityMetabolitesMarch 9, 2023Vanessa D’Antongiovanni, Matteo Fornai, Laura Benvenuti, Clelia Di Salvo, Carolina Pellegrini, Federica Cappelli, Stefano Masi, Luca AntonioliCell Signaling, Massachusetts, USA), occludin (ab167161, Abcam, Cambridge, UK), toll-like receptor (TLR)-4 (ab22048, Abcam, Cambridge, UK), nuclear factor kB p65 (NF-κB-p65, sc-8008, Santa Cruz, Dallas, USA) and myeloid-differentiation

(14) TLR4 Blockade Using Docosahexaenoic Acid Restores Vulnerability of Drug-Tolerant Tumor Cells and Prevents Breast Cancer Metastasis and Postsurgical RelapseACS Bio & Med Chem AuFebruary 15, 2023Mou Wang, Yuejing Wang, Renhe Liu, Ruilian Yu, Tao Gong, Zhirong Zhang, Yao Fuwith 1% (w/v) bovine serum albumin (Sigma-Aldrich) in PBS for 60 min. TLR4 antibody (1:100) (Abcam; ab22048) or NF-κB p65 antibody (1:400) (Cell Signaling, D14E12) (UniProtKB Q04206) served as primary reagents.

(15) Effects of bacterial and viral pathogen-associated molecular patterns (PAMPs) on multidrug resistance (MDR) transporters in brain endothelial cells of the developing human blood–brain barrierFluids and Barriers of the CNSJanuary 31, 2023Phetcharawan Lye, Enrrico Bloise, Stephen G. Matthewswere incubated with primary antibodies, TLR-3 (ab62566, 1:500, Abcam, Toronto, ON, Canada), TLR-4 (ab22048, 1;200, Abcam), TLR-8 (ab180610, 1:200, Abcam), von Willebrand Factor (vWF) (ab11713,1:500, Abcam),

(16) Effect of a combination of Atractylodes macrocephala extract with strychnine on the TLR4/NF‑κB/NLRP3 pathway in MH7A cellsExperimental and Therapeutic MedicineJanuary 9, 2023Yijing Gao, Dan Xin, Xiao-Dong Liang, Yingxue TangImmunofluorescence analysis The dilution, batch number and supplier of the primary antibodies used were: TLR4 (1:800; cat. no. ab22048; Abcam), NF-κBP65, (1:1,200; cat. no. ab16502; Abcam) and NLRP3 (1:800; cat. no. ab214185; Abcam). The

(17) Oxysophoridine protects against cerebral ischemia/reperfusion injury via inhibition of TLR4/p38MAPK‑mediated ferroptosisMolecular Medicine ReportsJanuary 4, 2023Jing Zhao, Mingming Ma, Lei Li, Gaoli Fangbrain tissue was incubated with primary antibodies against toll-like receptor (TLR)4 (1:1,000; cat. no. ab22048; Abcam) at 4°C overnight. The cells were exposed to DAPI (BIOSS) at room temperature for 5 min were

(18) Babao Dan decreases hepatocarcinogenesis by inhibiting hepatic progenitor cells malignant transformation via down-regulating toll-like receptor 4Frontiers in oncologyJanuary 1, 2023Liang L, Zhang LY, Liu WT, Zong C, Gao L, Li R, Zhao QD, Zhao NP, Wei LX, Zhang L, et al.The antibody of CK-19(10712-1-AP, proteintech), SOX-9(ab185230, Abcam), Toll-like receptor 4 (TLR4) (ab22048, Abcam), Ras (67648, CST), ERK (4695, CST), P-ERK (4370, CST) and GAPDH (AP0063, Bioworld) were used

(19) Effects of three types of fresh Rehmannia glutinosa improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathwayIran J Basic Med SciJanuary 1, 2023Meng Liu, Mengnan Zeng, Pengli Guo, Yuhan Zhang, Xiaofeng Yang, Jufang Jia, Qinqin Zhang, Beibei Zhang, Bing Cao, Ru Wang, et al.5% BSA blocking solution on a shaker for 1 hr. Then, we added the antibodies against TLR4 (1:2000, ab22048, Abcam, Cambridge, UK), MyD88 (1:500, ab135693, Abcam, Cambridge, UK), NF-κB p-p65 (1:5000, ab86299,

(20) TLR4 activation by lysozyme induces pain without inflammationFrontiers in immunologyJanuary 1, 2023Yadav S, Singh A, Kant R, Surolia A.#L2630), Glutamate assay kit (Sigma #MAK004) and Minocycline were purchased from Sigma. TLR4 (Abcam #ab22048), PGP9.5 (Abcam #ab108986), GOT (Abcam #ab221939) and Glutaminase (Abcam #ab156876) antibodies were

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10/26/2021TLR4 Antibody Supports Many Applications Wei Yang SitTLR4 antibody is used to detect for the changes in the protein expression level in macrophages.

Anti-TLR4 antibody [76B357.1] from Abcam

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Anti-TLR4 antibody [76B357.1] from Abcam

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