TLR4 Antibody (76B357.1) - BSA Free NB100-56566 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemTLR4 Antibody (76B357.1) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $469.00Supplier Page View Company Product Page
Catalog NumberNB100-56566
ImmunogenThis TLR4 Antibody (76B357.1) was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564)
Concentration1.0 mg/ml
TargetTLR4
Clone76B357.1
ApplicationsChemotaxis,Cytometric Bead Assay Standard,Flow Cytometry Control,IF/IHC,In-vitro,Flow Cytometry,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vitro assay,Dot Blot,In vivo assay,SDS-Page,Flow (Cell Surface),Flow (Intracellular),Simple Western,ELISA Capture (Matched Antibody Pair),Chromatin Immunoprecipitation (ChIP),CyTOF-ready, Knockdown Validated,Block/Neutralize,Dual RNAscope ISH-IHC,Immunocytochemistry/ Immunofluorescence
ClonalityMonoclonal
ReactivityBovine,Human,Mammal,Mouse,Porcine,Rat
IsotypeIgG2b Kappa
Conjugate/TagUnconjugated
HostMouse
Quantity0.1 mg (also 0.025 mg)
FormatPBS
PurityProtein G purified
Research AreaAdaptive Immunity, Autophagy, Cytokine Research, Immunology, Inflammation, Innate Immunity, Neurodegeneration, Signal Transduction, Virology Bacteria and Parasites
ValidationKnockout Validated
Antibody TypePrimary Antibodies
Molecular Weight95.7 kDa
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene Nametoll like receptor 4
NCBI Gene AliasesTLR-4, TOLL, CD284, ARMD10

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 mg (also 0.025 mg)

Questions and Answers

What species can this TLR4 antibody be used with in my experiments?

The TLR4 Antibody (76B357.1) is reactive with a wide range of species, including human, mouse, rat, canine, bovine, feline, horse, non-human primate, goat, hamster, guinea pig, porcine, donkey, rabbit, and sheep.

Has this antibody been used in any specific applications in research?

Yes, the TLR4 Antibody has been shown to work in various applications, including Western blotting, immunohistochemistry, and flow cytometry. For instance, it was utilized in a study examining TLR4 expression in multiple myeloma cell lines, where it helped elucidate the role of TLR4 in cell proliferation and apoptosis regulation ().

Can this antibody help in studying the effects of lipopolysaccharide on cells?

Yes, the TLR4 Antibody has been used in studies investigating the effects of lipopolysaccharide (LPS) on cells. For example, it was employed to assess TLR4 expression in porcine vascular wall-mesenchymal stem cells exposed to LPS, which demonstrated the antibody's utility in understanding inflammatory responses ().

Is there any evidence of this antibody being effective in animal models?

The TLR4 Antibody has been effectively used in animal models, such as in studies involving rats where it was used to analyze TLR4 expression in the context of neuroinflammation and migraine models. The antibody's application in these studies supports its relevance in understanding TLR4-mediated signaling pathways ().

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Cardiac Tissue Damage in a Female Animal Post-COVID Model: Relevance of Chemokine-Mediated InflammationVirusesDecember 16, 2025Silvia Flaj-Prados, Esperanza Herradón Pliego, Carlos Goicoechea Garcia, Eva M. Sánchez-Robles, Lars Arendt-Nielsen, César Fernández-de-Las-Peñas, Visitación López-Mirandafollowing antibodies: Toll-like receptor-4 (TLR4) 1:500 (Novus Biosciences, Toronto, ON, Canada, Cat#NB100-56566); Myeloid differentiation primary response gene 88 (MyD88) 1:500 (Abcam, Cambridge, UK, Cat#2064); Nuclear

(2) Glial activation and increase in cerebral pro-inflammatory cytokine expression in a female animal post-COVID modelBrain Behav Immun HealthDecember 1, 2025Silvia Flaj Prados, Esperanza Herradón Pliego, Carlos Goicoechea Garcia, Eva Ma Sánchez-Robles, Lars Arendt-Nielsen, Cesar Fernández-de-Las-Peñas, Visitación López-Mirandaovernight with the primary following antibodies: Toll-like receptor-4 (TLR4) 1:500 (Novus Biosciences Cat#NB100-56566); Myeloid differentiation primary response gene 88 (MyD88) 1:500 (Abcam, Cat#2064); Nuclear Factor kappa-light-chain-enhancer

(3) Microphysiological system to address the opioid crisis: A novel multi-organ model of acute opioid overdose and recoveryCurrent Research in ToxicologyJanuary 1, 2025Aakash Patel, Suruchi Poddar, Daniel Nierenberg, Stephanie Lang, Hao Wang, Camilly Pestana Pires DeMello, Julio Gamarra, Alisha Colon, Paula Kennedy, Jeffry Roles, et al.κ-opioid receptors (Santa Cruz Biotechnology, sc-374479), and anti-TLR4 for toll-like receptor 4 (Novus, NB100-56566). The next day, the primary antibody/blocking buffer solution was removed, and the cells were rinsed

(4) Ketogenic diet prevents paclitaxel-induced neuropathic nociception through activation of PPARγ signalling pathway and inhibition of neuroinflammation in rat dorsal root ganglionThe European journal of neuroscienceAugust 1, 2021Zhong S, Zhou Z, Lin X, Liu F, Liu C, Liu Z, Deng W, Zhang X, Chang H, Zhao C.

(5) Glia-Selective Deletion of Complement C1q Prevents Radiation-Induced Cognitive Deficits and NeuroinflammationCancer ResearchApril 1, 2021Mineh Markarian, Robert P. Krattli, Jabra D. Baddour, Leila Alikhani, Erich Giedzinski, Manal T. Usmani, Anshu Agrawal, Janet E. Baulch, Andrea J. Tenner, Munjal M. Acharya(1:200, Bio-Rad Cat# MCA1957GA, RRID:AB_324217), mouse anti-TLR4 (1:100, toll-like receptor 4, Novus Cat# NB100-56566, RRID:AB_2205129), rat anti-C5aR1 (1:500, Abcam Cat# ab117579, RRID:AB_10902319), mouse anti-GFAP

(6) Role of Nociceptor Toll-like Receptor 4 (TLR4) in Opioid-Induced Hyperalgesia and Hyperalgesic PrimingThe Journal of NeuroscienceAugust 14, 2019Dioneia Araldi, Oliver Bogen, Paul G. Green, Jon D. Levineh at room temperature, cut in half at ⬃75 kDa, and probed with either a mouse monoclonal anti-TLR4 (NB100 – 56566, 1:500, Novus Biologicals) or a rabbit polyclonal anti ␤-actin (ab8227, 1:1000, Abcam) antibody in antibody

(7) Xuezhikang improves the outcomes of cardiopulmonary resuscitation in rats by suppressing the inflammation response through TLR4/NF-κB pathwayBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapieJune 1, 2019Liang L, Shao W, Shu T, Zhang Y, Xu S, Guo L, Zhou Y, Huang H, Sun P.

(8) Acute inflammation down-regulates alpha-synuclein expression in enteric neuronsJournal of neurochemistryMarch 1, 2019Prigent A, Gonzales J, Durand T, Le Berre-Scoul C, Rolli-Derkinderen M, Neunlist M, Derkinderen P.respectively), and mouse monoclonal anti-toll-like receptor 4 (TLR4) (Novus, Bio-Techne, Lille, France, Cat# NB10056566, RRID:AB_2205129). Bound antibodies were detected with horseradish peroxidase-conjugated anti-rabbit

(9) CEACAM1 regulates the IL-6 mediated fever response to LPS through the RP105 receptor in murine monocytesBMC immunologyJanuary 23, 2019Zhang Z, La Placa D, Nguyen T, Kujawski M, Le K, Li L, Shively JE.

(10) Bushen recipe and its disassembled prescriptions inhibit inflammation of liver injury associated with Concanavalin A through Toll‑like receptor 3/9 signaling pathwayMolecular medicine reportsAugust 1, 2018Nie H, Mei Z, Wang R, Zhao B, Gao Y, Chen J, Wang L.Germany). The primary antibodies used in the western blot analysis were as follows: Anti‑TLR4 (cat. no. NB100‑56566), anti‑TLR3 (cat. no. NB100‑56571) and anti‑TLR9 (cat. no. nbp2‑24729), purchased from Novus Biologicals

(11) Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent mannerJournal of neuroinflammationNovember 25, 2017Sun L, Li M, Ma X, Feng H, Song J, Lv C, He Y.

(12) Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivoJournal of immunology (Baltimore, Md. : 1950)April 15, 2015Martin I, Cabán-Hernández K, Figueroa-Santiago O, Espino AM.

(13) Toll-Like Receptor 4 Activation Promotes Multiple Myeloma Cell Growth and Survival Via Suppression of The Endoplasmic Reticulum Stress Factor ChopScientific reportsJanuary 1, 0001"Bagratuni T, Sklirou AD, Kastritis E, Liacos CI, Spilioti C, Eleutherakis-Papaiakovou E, Kanellias N, Gavriatopoulou M, Terpos E, Trougakos IP, et al."antibodies at room temperature. The primary antibodies used were: TLR4 (Novus Biologicals, cat no: NB100-56566), CHOP (Santa Cruz, cat no: sc-575), p-eIF2A (Millipore, cat no: 07-760), eIF2A (Cell Signaling Technology,

(14) Inhibitory effect of Patrinia on BRL-3A cell apoptosis through the TLR4/PI3K/AKT/GSK3β and TLR4/P38/JNK signaling pathwaysMolecular medicine reportsJanuary 1, 0001"Chen X, Yan X, Guo L."

(15) Impact of heat stress during the follicular phase on porcine ovarian steroidogenic and phosphatidylinositol-3 signalingJournal of animal scienceJanuary 1, 0001"Dickson MJ, Hager CL, Al-Shaibi A, Thomas PQ, Baumgard LH, Ross JW, Keating AF."

(16) Improvement of Colonic Immune Function with Soy Isoflavones in High-Fat Diet-Induced Obese Rats"Molecules (Basel, Switzerland)"January 1, 0001"Luo Q, Cheng D, Huang C, Li Y, Lao C, Xia Y, Liu W, Gong X, Hu D, Li B, et al."

(17) Cocaine self-administration differentially activates microglia in the mouse brainNeuroscience lettersJanuary 1, 0001"Burkovetskaya ME, Small R, Guo L, Buch S, Guo ML."

(18) Dasatinib regulates LPS-induced microglial and astrocytic neuroinflammatory responses by inhibiting AKT/STAT3 signalingJournal of neuroinflammationJanuary 1, 0001"Ryu KY, Lee HJ, Woo H, Kang RJ, Han KM, Park H, Lee SM, Lee JY, Jeong YJ, Nam HW, et al."

(19) Activation of TNFR1 and TLR4 following oxygen glucose deprivation promotes mitochondrial fission in C6 astroglial cellsCellular signallingJanuary 1, 0001"Halder A, Yadav K, Aggarwal A, Singhal N, Sandhir R."

(20) "PC1/3 KD Macrophages Exhibit Resistance to the Inhibitory Effect of IL-10 and a Higher TLR4 Activation Rate, Leading to an Anti-Tumoral Phenotype"CellsJanuary 1, 0001"Rodet F, Capuz A, Ozcan BA, Le Beillan R, Raffo-Romero A, Kobeissy F, Duhamel M, Salzet M."

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