Nucleic Acid Quantification Kit From Thermo Fisher is Perfect for the Detection and Labelling of RNA

January 09, 2024

BlueskyReddit
Covaris
R&D
Associate Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

Quant-it™ RiboGreen RNA Assay Kit and RiboGreen RNA Reagent, RediPlate™ 96 RiboGreen™ RNA Quantitation Kit

Catalog Number:

R11490

Ribogreen assay, used in quantification of RNA. In this case, it was used for FFPE RNA quantitation. The sample was in H2O.

Experimental Design and Results Summary

Application

Assessment of extraction quality

Starting Material

RNA from FFPE in Tris solution

Tips

Ensure to dilute into correct range of purchase the correct kit (BR or HS)

Results Summary

Simply put, this was used to quantify RNA in a H2OO buffer after being extracted from FFPE from 10 tissue types.

DOI or PMID #

N/A

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Summary

The Good

Optimal for 16 samples or more.

The Bad

Requires plate reader and correct plates.

The Bottom Line

Overall a great product for 16 samples or more. Standard curve is similar to Picogreen and can be tedious to make but is a great quantification kit.

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