Decent Anti-Mouse PD-L1 (PE) From Thermo Fisher

January 08, 2024

BlueskyReddit
Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

Anti-mouse CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5)

Catalog Number:

14-5982-82

Clone Number:

MIH5

This antibody provides good staining of PD-L1-positive populations in mouse intestinal immune caecum cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse caecum cells

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

Good staining of PD-L1 positive cells in mouse intestinal cells. Great for detecting out the cells than can interact with exhausted PD-1 positive cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good and specific staining.

The Bad

Require antibody titration to improve the staining at the best.

The Bottom Line

Decent staining of PD-L1 in mouse intestinal immune cells. This markers usually provide weak staining, so I would recommend titrating for reducing background.

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