This Protein Labeling Reagent From Invitrogen Makes it Easy to Visualize Proteins

January 08, 2024

BlueskyReddit
UNC Chapel Hill

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Company:

Thermo Fisher

Product Name:

No-Stain™ Protein Labeling Reagent

Catalog Number:

A44717

The Invitrogen No-Stain Protein Labeling Reagent provides a flexible, accurate, rapid, and reliable method to visualize and normalize proteins in a gel or on a membrane (post-transfer)

Experimental Design and Results Summary

Application

Western blot: protein visualization

Starting Material

Western blot membrane/lysates

Tips

I use the dilution as recommended

Results Summary

Coomassie and other gel staining and de-staining steps can be extremely time consuming and cumbersome. No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins in a gel within 10 minutes.

DOI or PMID #

N/A

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Summary

The Good

Fast.

The Bad

Can't think of anything bad.

The Bottom Line

Alternative to traditional gel staining reagents—provides more accurate normalization over a wide range of protein lysate concentrations (1–80 µg). Very sensitive, lower limit of detection of 20 ng per band. specifically forms bonds only with the lysine side chains of proteins.

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