NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(81) Transcriptome Analysis of the Late-Acting Self-Incompatibility Associated with RNase T2 Family in Camellia oleiferaPlants (Basel)May 9, 2023Chang Li, Mengqi Lu, Junqin Zhou, Sen Wang, Yi Long, Yan Xu, Xiaofeng Tanenriched mRNA was fragmented into short fragments using NEBNext® Magnesium RNA Fragmentation Module (NEB#E6150S, New England Biolabs, Ipswich, MA, USA) for 5 min at 94 °C and reverse transcribed into cDNA with random

(82) Transcriptome analysis reveals a potential regulatory mechanism of the lnc-5423.6/IGFBP5 axis in the early stages of mouse thymic involutionActa Biochimica et Biophysica SinicaApril 25, 2023Bingxin Li, Yaqiong Ye, Longsheng Hong, Wanyan Li, Qingru Wu, Wenjun Liu, Yongjiang Ma, Danning Xu, Yugu Lifragments using divalent cations under high temperature with the Next® Magnesium RNA Fragmentation Module (E6150S; NEB, Ipswich, USA). Then, the cleaved RNA fragments were reverse-transcribed to generate cDNA using

(83) β-Glucan Enhances the Biocontrol Efficacy of Marine Yeast Scheffersomyeces spartinae W9 against Botrytis cinerea in StrawberriesJournal of FungiApril 15, 2023Xueyan Chen, Yingying Wei, Xiurong Zou, Zichang Zhao, Shu Jiang, Yi Chen, Feng Xu, Xingfeng Shaofragmented with a magnesium ion interruption kit (NEBNextR Magnesium RNA Fragmentation Module, cat. E6150S, New England Biolabs, MA, USA). The fragmented RNA was synthesized into cDNA. Then, double-stranded

(84) Integration of mRNA and miRNA Analysis Reveals the Post-Transcriptional Regulation of Salt Stress Response in Hemerocallis fulvaInternational Journal of Molecular SciencesApril 14, 2023Bo Zhou, Xiang Gao, Fei ZhaoUSA). Next, the mRNA was fragmented into small pieces via Magnesium RNA Fragmentation Module (NEB, cat. E6150S, USA). Then, SuperScript™ II Reverse Transcriptase (Invitrogen, cat. 1896649, USA) was used to reverse-transcribe

(85) Continuous transcriptome analysis reveals novel patterns of early gene expression in Drosophila embryosCell GenomicsMarch 8, 2023J. Eduardo Pérez-Mojica, Lennart Enders, Joseph Walsh, Kin H. Lau, Adelheid LempradlMEGAscript™ T7 Transcription Kit,Thermo Fisher Scientific,Cat#AM1333 NEBNext® Magnesium RNA Fragmentation Module,New England Biolabs,Cat#E6150S ,, Deposited data,Deposited data,Deposited data ,, Zelda-dependent/independent and H2A.Z-positive/negative

(86) Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA m(3)C modificationCell stem cellMarch 2, 2023Zhang F, Yoon K, Zhang DY, Kim NS, Ming GL, Song H.modification sites13. 8 μg total RNA was fragmented by incubating in 20 μl of RNA fragmentation reagents (NEB, E6150S) at 95°C for 4 mins. The fragmentation reaction was stopped by adding 2 μl 10X RNA Fragmentation Stop

(87) Iberverin exhibits antineoplastic activities against human hepatocellular carcinoma via DNA damage-mediated cell cycle arrest and mitochondrial-related apoptosisFrontiers in pharmacologyJanuary 1, 2023Zhang Y, Du J, Jin L, Pan L, Yan X, Lin S.preparations using a NEBNext® Magnesium RNA Fragmentation Module (NEB, the United States, catalog no. E6150S). Subsequently, paired-end sequencing was performed on Illumina NovaseqTM 6000 by LC-Bio Technology

(88) Limited effects of m6A modification on mRNA partitioning into stress granulesNature CommunicationsDecember 1, 2022Anthony Khong, Tyler Matheny, Thao Ngoc Huynh, Vincent Babl, Roy Parkerusing Trizol extraction. The RNA was then fragmented using NEBNext Magnesium RNA fragmentation module (E6150S; NEB) following the manufacturer’s protocol. The reaction was cleaned using RNeasy Mini kit (74106;

(89) A multiplex platform for small RNA sequencing elucidates multifaceted tRNA stress response and translational regulationNature CommunicationsDecember 1, 2022Christopher P. Watkins, Wen Zhang, Adam C. Wylder, Christopher D. Katanski, Tao PanBriefly, polyA+ RNA in 18 µl were added to PCR tubes and 2 µl Magnesium RNA fragmentation buffer (NEB, E6150S) was added to each tube. The tubes were incubated at 94 °C in a thermocycler for 5 min to fragment the

(90) Detection of m6A RNA modifications at single-nucleotide resolution using m6A-selective allyl chemical labeling and sequencingSTAR ProtocolsDecember 1, 2022Yong Peng, Hanzhe Meng, Ruiqi Ge, Shun Liu, Mengjie Chen, Chuan He, Lulu HuQubit™ RNA HS Assay Kit,Thermo Fisher Scientific,Cat#Q32852 NEBNext® Magnesium RNA Fragmentation Module,New England Biolabs,Cat#E6150S Oligo(dT)18 Primer,Thermo Fisher Scientific,Cat#SO132 RNase H and 10 × RNase H Reaction Buffer,New England Biolabs

(91) Multi-omics analysis reveals GABAergic dysfunction after traumatic brainstem injury in ratsFrontiers in NeuroscienceNovember 23, 2022Qin Su, Qianling Chen, Zhigang Li, Jian Zhao, Lingyue Li, Luyao Xu, Bin Yang, Chao Liuhigh temperature using the Magnesium ion Fragmentation kit (NEBNextR RNA Fragmentation Module, CAT.e6150s, New England Biolabs, Ipswich, MA, United States). Reverse transcription of mRNA fragments was performed

(92) Mettl3-dependent m⁶A modification attenuates the brain stress response in DrosophilaNature communicationsSeptember 14, 2022Perlegos AE, Shields EJ, Shen H, Liu KF, Bonini NM.NEBNext Poly(A) mRNA Magnetic Isolation Module. PolyA+ RNA was fragmented using the NEB Next Magnesium Fragmentation Module (NEB, E6150S) for 4 min at 95 °C for a 250 ng sample of polyA+ RNA, and RNA was repurified using the Zymo RNA clean

(93) CRHR1 mediates the transcriptional expression of pituitary hormones and their receptors under hypoxiaFront Endocrinol (Lausanne)September 2, 2022Tong Ying Wang et al.Then the poly(A) RNA was fragmented into small pieces using the Mg2+ RNA fragmentation module (Cat. E6150S, NEB, USA) at 94°C for 5–7 min. Then the cleaved RNA fragments were reverse-transcribed to synthesize

(94) Socializing Models During Lactation Alter Colonic Mucosal Gene Expression and Fecal Microbiota of Growing PigletsFrontiers in MicrobiologyJuly 7, 2022Yanju Bi, Haidong Wei, Haoyang Nian, Runze Liu, Wenbo Ji, Honggui Liu, Jun Baocaptured mRNA was fragmented into small pieces using a Magnesium RNA Fragmentation Module (NEB cat.e6150S, USA) at 94°C for 5–7 min, and the cleaved RNA fragments were then reverse-transcribed to create the

(95) m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translationCell discoveryMay 21, 2022Zhuo W, Sun M, Wang K, Zhang L, Li K, Yi D, Li M, Sun Q, Ma X, Liu W, et al.overnight. Three μg total RNA was fragmented to ~150 nt using magnesium RNA fragmentation buffer (NEB, E6150S), and ethanol precipitated. The fragmented RNA was denatured at 65 °C for 5 min and chilled on ice immediately,

(96) Laser Microdissection for Species-Agnostic Single-Tissue ApplicationsJournal of Visualized ExperimentsApril 18, 2022Alyssa Woronik, Karin Kiontke, Raya S. Jallad, R. Antonio Herrera, David H. A. FitchGCCTTGGCACCCGAGAATTCCANNNNNN,IDT,,a primer with 6 nucleotides that are random RNA Fragmentation buffer,NEB,E6150S, RNA Fragmentation stop buffer,NEB,E6150S, RNA PCR Index Primers (RPI1–RPI48),"Illumina, NEB, or IDT",,"RPIX

(97) Loss of Slc38a4 imprinting is a major cause of mouse placenta hyperplasia in somatic cell nuclear transferred embryos at late gestationCell reportsFebruary 22, 2022Xie Z, Zhang W, Zhang Y.RNase-free",ThermoFisher Scientific,Cat#EN0521 NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,Cat#E6150S Second Strand Buffer,ThermoFisher Scientific,Cat#10812014 Magnesium Chloride (MgCl2),Sigma-Aldrich,Cat#M1028 Dithiothreitol (DTT

(98) Profiling Selective Packaging of Host RNA and Viral RNA Modification in SARS-CoV-2 Viral PreparationsFrontiers in Cell and Developmental BiologyFebruary 3, 2022Noah Peña et al.fragmentation experiment. Eluted RNA was then transferred to PCR tubes, 2 µl RNA fragmentation buffer (E6150S, NEB) was added to each tube and mixed well. Samples were incubated at 95°C on PCR machine for 6 min

(99) Modifying the m(6)A brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic taggingMolecular psychiatryDecember 1, 2021Martinez De La Cruz B, Markus R, Malla S, Haig MI, Gell C, Sang F, Bellows E, Sherif MA, McLean D, Lourdusamy A, et al.(Illumina, MRZG12324). Ribo-depleted RNA was fragmented to 100 bp using NebNext Fragmentation module (NEB, E6150S). 1/10 volume of fragmented RNA was separated to use as input control. 5 μg of m6A Ab (abcam, ab151230)

(100) The spatial RNA integrity number assay for in situ evaluation of transcriptome qualityCommunications BiologyDecember 1, 2021Linda Kvastad et al.was performed using NEBNext Magnesium RNA Fragmentation Module according to provided instructions (E6150S, New England Biolabs, NEB), at 94 °C for 10, 30 s, 2 or 4 min. Reverse transcription in solution for

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NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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