NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) PINK1 overexpression suppresses p38 MAPK/NF‑κB signaling to attenuate chondrocyte senescence in osteoarthritisInt J Mol MedOctober 15, 2025Lishi Jie, Yuanhui Zhang, Jiangyu Liu, Houyu Fu, Zaishi Zhu, Zeling Huang, Xiaoqing Shi, Peimin Wang, Songjiang Yinfragmentation buffer with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, cat. no. E6150S). First-strand cDNA was synthesized using random hexamer primers (50 μM; Invitrogen, cat. no. N8080127)

(42) Integrated 16 S rRNA and transcriptome analysis reveal molecular and microbial mechanisms of cold-tolerant germination in hulless barleyBMC GenomicsOctober 10, 2025Panrong Ren, Jie Wang, Lei Gongwere fragmented at 94℃ for 5–7 min using the NEBNextR Magnesium RNA Fragmentation Module (NEB, cat. E6150S, USA). Subsequently, the fragmented RNA (1 µg, 50 ng/µL) underwent reverse transcription using the Invitrogen

(43) α-hederin inhibits cervical cancer progression by inducing DNA damage-dependent cell cycle blockade and apoptosisBMC cancerOctober 3, 2025Song Y, Qin H, Huang S, Wang J, Huang Y, Lin Z, Yang F, Zhang X, Wang R, Wang J.USA), with RNase H (NEB, No. m0209, USA), and RNase H (NEBNext® Magnesium RNA Fragmentation Module, No. E6150S, USA). RNase H (NEB, item no. m0297, USA) for two-strand synthesis to convert these composite double

(44) Coupled transcriptome and proteome analysis of L3 and L4 developmental stages of Anisakis simplex s. s.: insights into target genes under glucose influenceBMC GenomicsSeptember 29, 2025Iwona Polak, Robert Stryiński, Mateusz Maździarz, Lukasz Paukszto, Mónica Carrera, Iwona Bogacka, Elżbieta Łopieńska-Biernatmanufacturer’s instructions. After removal of the ribosomal RNAs, the remaining RNAs were fragmented (Cat. No. E6150S, NEBNext® Magnesium RNA Fragmentation Module, New England Biolabs, Ipswich, MA, USA) into short fragments

(45) Lysosomal RNA profiling reveals targeting of specific types of RNAs for degradationbioRxivSeptember 16, 2025G. Jordan Ray, Eleonora Nardini, Heather R. Keys, Daniel H. Lin, David M. Sabatini, David P. Bartelconcentrators kits as above. Then, RNA was fragmented for 7 min at 94°C using NEB RNA fragmentation module (E6150S), before again purifying RNA using Trizol LS and zymo-RNA clean and concentrators kits as above. Then,

(46) METTL3/ALKBH5‐Mediated N6‐Methyladenosine Modification Drives Macrophage M1 Polarization via the SLC15A3‐TASL‐IRF5 Signaling Axis in PsoriasisAdvanced ScienceSeptember 1, 2025Tao Huang, Shijun Chen, Ke Ding, Liyan Yuan, Weiqi Lv, Kechen Chen, Yuchen Liu, Dongzhao Ma, Xin Zhang, Xiaobo Wang, et al.eluted RNA was fragmented at 94°C for 30 seconds using RNA Fragmentation Reagents (New England Biolabs, E6150S) and purified using the ZYMO RNA Clean & Concentrator Kit (Zymo Research, R1016). Proceed with the m6A

(47) Injectable sustained-release danshensu sodium-loaded nanoparticle hydrogel targets macrophages to improve myocardial microenvironment for myocardial infarction treatmentBioact MaterAugust 17, 2025Jianqiu Yang, Jing Wang, Zhihua Zeng, Zhenyu Chen, Dingguo Wang, Yongbing Wu(NEB, No. m0209, USA) and RNase H (NEB, no. m0209; NEBNext® Magnesium RNA Fragmentation Module, No. E6150S, USA; and NEB, item no. m0297, USA) was used for second - strand synthesis, converting DNA - RNA hybrids

(48) Identification of NPR2 gene mutations affecting chondrocyte differentiation in short stature through JAK2-STAT5Orphanet J Rare DisAugust 1, 2025Shuoshuo Wei, Mingming He, Chuanpeng Zhang, Yanying Li, Mei Zhang, Xinguo Hou, Bo Ban, Qianqian ZhaoOligo (dT) (Thermo Fisher, USA) and fragmented using a magnesium-based fragmentation kit (NEB, cat.e6150s, USA) at 94 °C for 5–7 min. The fragmented mRNA fragments were then reverse transcribed using SuperScript™ II Reverse Transcriptase

(49) Association of alterations in transcriptomics and intestinal immune responses with Bifidobacterium longum BAA2573 in improving dextran sulfate sodium-induced colitisBMC GastroenterolAugust 1, 2025Wujuan Hao, Lan Gu, Renmin Zhou, Cuilan Huang, Xuyang Wang, Yanshan Liu, Qiong Lincaptured mRNA was segmented at high temperatures (94 ℃, 6 min) using a magnesium ions interrupt kit (Cat.E6150S, NEB, USA). RNA fragments were synthesized into double-stranded cDNA using reverse transcriptase (Cat.1896649,

(50) The PLEKHA1-TACC2 fusion gene drives tumorigenesis via vascular mimicry formation in esophageal squamous-cell carcinomaCell Death & DifferentiationJuly 5, 2025Ting Yang, Zhi-Rui Lin, Tian-Liang Xia, Shang-Xin Liu, Bo-Yu Yuan, Yi-Ling Luo, Wen-Ting Du, Chao-Bo Lei, Yong-Zhan Nie, Mu-Sheng Zeng, et al.(ThermoFisher; EN0521) and then randomly fragmented via fragmentation buffer (New England Biolabs; E6150S). The first and second cDNA strand were synthesized with random hexamer primers by using Superscript

(51) Stress granule assembly impairs macrophage efferocytosis to aggravate allergic rhinitis in miceNat CommunJuly 1, 2025Ye Zhou, Zixuan Yang, Yuanyuan Wang, Yue Dong, Tianyu Wang, Yunhui Li, Caiquan Liang, Yanfang Liu, Zhixuan Li, Shanrong Liu, et al.(ab104139) were purchased from Abcam (Cambridge, UK). NEB Next Magnesium RNA Fragmentation Module (E6150S) and T4 polynucleotide kinase (M0201S) were purchased from New England Biolabs (Ipswich, MA). Tobacco

(52) Small-molecule-catalysed deamination enables transcriptome-wide profiling of N6-methyladenosine in RNANature ChemistryJuly 1, 2025Pingluan Wang, Chang Ye, Michelle Zhao, Bochen Jiang, Chuan Heextracted from total RNA and 10–20 ng of the RNA was fragmented using RNA fragmentation reagents (NEB, E6150S) at 94 °C for 2 min, followed by purification with the RNA Clean and Concentrator kit (Zymo Research).

(53) N6-methyladenosine-modified GPX2 impacts cancer cell stemness and TKI resistance through regulating of redox metabolismCell Death DisJune 18, 2025Xu Yang, Long Yu, Miaomiao Shao, Huiling Yang, Kangwei Qi, Gaofei He, Lanxin Wang, Di Kong, Jianxin Gu, Xiaolin Xu, et al.15596026CN). RNA was randomly fragmented by using NEBNext® Magnesium RNA Fragmentation Modul (NEB, E6150S). Anti-IgG and anti-m6A (Cell Signaling Technology, 56593) antibodies were conjugated to Protein G-Agarose beads

(54) Zinc sulfate improves insulin resistance, oxidative stress and apoptosis in liver tissues of PCOS rats through the NF-κB pathwayFront Endocrinol (Lausanne)June 6, 2025Yi-Fan Kang, Jia-Yi Zhao, Jian-Rong Liuat high temperature using the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, cat. E6150S) for 5–7 minutes at 94°C. Subsequently, the fragmented RNA was reverse transcribed into cDNA using Invitrogen

(55) CRISPR screen decodes SWI/SNF chromatin remodeling complex assemblyNat CommunMay 30, 2025Hanna Schwaemmle, Hadrien Soldati, Nikolaos M. R. Lykoskoufis, Mylène Docquier, Alexandre Hainard, Simon M. G. BraunIsolation kit (NEB, S1550S). mRNA was fragmented using the NEBNext Magnesium RNA fragmentation module (NEB, E6150S) and purified using Monarch RNA Cleanup Kit (NEB, T2030S). 100 ng mRNA was aliquoted and stored as input

(56) Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas diseaseBMC GenomicsMay 5, 2025Lei Duan, Yunjia Tian, Ziyi Wang, Limin Yang, Yunhai Guo, Yuanyuan Li, Zhengbin Zhou, Yong Shen, Yi Zhang, Qin Liufragments via chemical hydrolysis using the NEBNext® Magnesium RNA Fragmentation Module (NEB, Cat# E6150S) at 94 °C for 8 min. First-strand cDNA synthesis was performed with random hexamer primers and SuperScript™ IV Reverse Transcriptase

(57) A nuclear RNA degradation code is recognized by PAXT for eukaryotic transcriptome surveillanceMolecular CellApril 17, 2025Lindsey V. Soles, Liang Liu, Xudong Zou, Yoseop Yoon, Shuangyu Li, Lusong Tian, Marielle Valdez, Angela M Yu, Hong Yin, Wei Li, et al.Lipofectamine RNAiMAX Transfection Reagent,Invitrogen,Cat#13778100 NEBNext Magnesium RNA fragmentation module,NEB,Cat#E6150S Phusion High-Fidelity PCR Master Mix with HF Buffer,NEB,Cat#M0531L Lonza SF Cell Line 4D-Nucleofector

(58) Treatment of Pathological Lymphangiogenesis via Circular RNA-Mediated Cholesterol Metabolism RemodelingInvest Ophthalmol Vis SciApril 11, 2025Shuting Lu, Qian Liu, Fan Ye, Ziran Zhang, Lianjun Shi, Xiumiao Li, Wan Mu, Qin Jiang, Biao Yan(Thermo Fisher Scientific). Subsequently, the poly(A) RNA was fragmented using the Magnesium RNA Fragmentation Module (E6150S; New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were reverse transcribed into complementary

(59) Targeting PRDX2 to inhibit tumor growth and metastasis in triple-negative breast cancer: the role of FN1 and the PI3K/AKT/SP1 pathwayJ Transl MedApril 11, 2025Jiawei Zhou, Anqi Cheng, Jianqiang Guo, Yafeng Liu, Xuan Li, Maoqian Chen, Dong Hu, Jing WuThermo Fisher) in two rounds. Fragmentation was performed with NEB Next® Magnesium RNA Fragmentation Module (#E6150S) at 94 °C for 5–7 min. cDNA synthesis used Super Script™ II Reverse Transcriptase (#1896649, Thermo

(60) Decoding m6Am by simultaneous transcription-start mapping and methylation quantificationElifeMarch 31, 2025Jianheng Fox Liu, Ben R Hawley, Luke S Nicholson, Samie R Jaffreyfragmentized the input RNA into ~200 nt long fragments (NEBNext Magnesium RNA Fragmentation Module (NEB #E6150S), 94 °C, 2 min). The fragmentized RNAs were then A-to-I converted based on the GLORI protocol (Liu et

Be the First to Write a Review!

NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

New England Biolabs

Citations (148)

Reviews

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map