NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) Zinc sulfate improves insulin resistance, oxidative stress and apoptosis in liver tissues of PCOS rats through the NF-κB pathwayFront Endocrinol (Lausanne)June 6, 2025Yi-Fan Kang, Jia-Yi Zhao, Jian-Rong Liuat high temperature using the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, cat. E6150S) for 5–7 minutes at 94°C. Subsequently, the fragmented RNA was reverse transcribed into cDNA using Invitrogen

(42) CRISPR screen decodes SWI/SNF chromatin remodeling complex assemblyNat CommunMay 30, 2025Hanna Schwaemmle, Hadrien Soldati, Nikolaos M. R. Lykoskoufis, Mylène Docquier, Alexandre Hainard, Simon M. G. BraunIsolation kit (NEB, S1550S). mRNA was fragmented using the NEBNext Magnesium RNA fragmentation module (NEB, E6150S) and purified using Monarch RNA Cleanup Kit (NEB, T2030S). 100 ng mRNA was aliquoted and stored as input

(43) Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas diseaseBMC GenomicsMay 5, 2025Lei Duan, Yunjia Tian, Ziyi Wang, Limin Yang, Yunhai Guo, Yuanyuan Li, Zhengbin Zhou, Yong Shen, Yi Zhang, Qin Liufragments via chemical hydrolysis using the NEBNext® Magnesium RNA Fragmentation Module (NEB, Cat# E6150S) at 94 °C for 8 min. First-strand cDNA synthesis was performed with random hexamer primers and SuperScript™ IV Reverse Transcriptase

(44) A nuclear RNA degradation code is recognized by PAXT for eukaryotic transcriptome surveillanceMolecular CellApril 17, 2025Lindsey V. Soles, Liang Liu, Xudong Zou, Yoseop Yoon, Shuangyu Li, Lusong Tian, Marielle Valdez, Angela M Yu, Hong Yin, Wei Li, et al.Lipofectamine RNAiMAX Transfection Reagent,Invitrogen,Cat#13778100 NEBNext Magnesium RNA fragmentation module,NEB,Cat#E6150S Phusion High-Fidelity PCR Master Mix with HF Buffer,NEB,Cat#M0531L Lonza SF Cell Line 4D-Nucleofector

(45) Targeting PRDX2 to inhibit tumor growth and metastasis in triple-negative breast cancer: the role of FN1 and the PI3K/AKT/SP1 pathwayJ Transl MedApril 11, 2025Jiawei Zhou, Anqi Cheng, Jianqiang Guo, Yafeng Liu, Xuan Li, Maoqian Chen, Dong Hu, Jing WuThermo Fisher) in two rounds. Fragmentation was performed with NEB Next® Magnesium RNA Fragmentation Module (#E6150S) at 94 °C for 5–7 min. cDNA synthesis used Super Script™ II Reverse Transcriptase (#1896649, Thermo

(46) Treatment of Pathological Lymphangiogenesis via Circular RNA-Mediated Cholesterol Metabolism RemodelingInvest Ophthalmol Vis SciApril 11, 2025Shuting Lu, Qian Liu, Fan Ye, Ziran Zhang, Lianjun Shi, Xiumiao Li, Wan Mu, Qin Jiang, Biao Yan(Thermo Fisher Scientific). Subsequently, the poly(A) RNA was fragmented using the Magnesium RNA Fragmentation Module (E6150S; New England Biolabs, Ipswich, MA, USA). The cleaved RNA fragments were reverse transcribed into complementary

(47) PCIF1 drives oesophageal squamous cell carcinoma progression via m6Am-mediated suppression of MTF2 translationClinical and translational medicineApril 1, 2025Li K, Yi Y, Ling R, Zhang C, Zhang Z, Wang Y, Wang G, Chen J, Cheng M, Chen S.extracted RNA (∼10 µg) was fragmented to obtain ∼100–200 nt fragments using RNA fragmentation buffer (E6150S; NEB) at 70°C for 7.5 min. The fragmented RNAs were then subjected to m7G immunoprecipitation using

(48) Decoding m6Am by simultaneous transcription-start mapping and methylation quantificationElifeMarch 31, 2025Jianheng Fox Liu, Ben R Hawley, Luke S Nicholson, Samie R Jaffreyfragmentized the input RNA into ~200 nt long fragments (NEBNext Magnesium RNA Fragmentation Module (NEB #E6150S), 94 °C, 2 min). The fragmentized RNAs were then A-to-I converted based on the GLORI protocol (Liu et

(49) Transcriptome Sequencing Analysis of the Effects of Metformin on the Regeneration of Planarian Dugesia japonicaGenes (Basel)March 22, 2025Zelong Zhao, Dandan Yin, Kexin Yang, Chunmei Zhang, Linxia Song, Zhenbiao XuLibrary Prep Kit (NEB #E7530L), Oligo d (T) Magnetic Beads (NEB #S1419S), Fragmentation Buffer (NEB #E6150S), M-MuLV reverse transcriptase (NEB #M0253S) and RNase H (NEB #M0297S) were purchased from New England

(50) Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seqSTAR ProtocolsMarch 1, 2025Xiaoting Zhang, Liang Lu, Chengqi Yi, Xiaoyu LiSuperMix,Vazyme,Cat#R323-01 KAPA HiFi HotStart ReadyMix,KAPA,Cat#KR0370 NEBNext Magnesium RNA Fragmentation Module,NEB,Cat#E6150S T4 polynucleotide kinase,Vazyme,Cat#N102-01 "T4 RNA ligase 2, truncated",Beyotime,Cat#R0635L "T4

(51) Exploration of Conditions for the Scaled Application of Laparoscopic Ovum Pick-Up in Sheep and Comparison of Follicular Development Differences Among BreedsInternational Journal of Molecular SciencesFebruary 25, 2025Dongxu Li, Xiangli Wu, Ying Chen, Yangsheng Wu, Gulimire Abudureyimu, Hongyang Liang, Xiuling Ma, Wei Zhang, Liqin Wang, Jiapeng Linunder high-temperature conditions using the NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. E6150S, Ipswich, MA, USA). The fragmented RNA was reverse transcribed into complementary DNA (cDNA) using Invitrogen

(52) Transcriptome Profiling Reveals That the African Swine Fever Virus C315R Exploits the IL-6 STAT3 Signaling Axis to Facilitate Virus ReplicationVirusesFebruary 24, 2025Shuxian Geng, Zhonghui Zhang, Jie Fan, Hualin Sun, Jifei Yang, Jianxun Luo, Guiquan Guan, Hong Yin, Qiaoying Zeng, Qingli NiumRNA was fragmented into 200 bp fragments using the NEB Next Magnesium RNA Fragmentation Module (NEB, E6150S, Ipswich, MA, USA) and served as templates for double-stranded cDNA (ds cDNA) synthesis using the ds

(53) Protocol for measuring the auxin-induced changes of m6A modificationSTAR ProtocFebruary 4, 2025Bin Li, Yixing Li, Lei Li, Linjun Cai, Lan Li, Li Li, Chongsheng HeEN0525 Nitrocellulose membrane,Pall Corporation,Cat# 69095847 BSA,Sigma,Cat# B667 RNA fragment module,NEB,E6150S Protein-G beads,Thermo Fisher Scientific,Cat# 88848 IGEPAL CA-630,Sigma,Cat# I8896 m6A,Sigma,Cat#

(54) Machine learning-optimized targeted detection of alternative splicingNucleic Acids ResearchJanuary 24, 2025Kevin Yang, Nathaniel Islas, San Jewell, Di Wu, Anupama Jha, Caleb M Radens, Jeffrey A Pleiss, Kristen W Lynch, Yoseph Barash, Peter S ChoiNext, 2.67 µl of 10× RNA Fragmentation Buffer from the NEBNext Magnesium RNA Fragmentation Module (#E6150S, NEB) was added, resulting in a total volume of 26.67 µl. The reaction was incubated in a preheated

(55) BASAL: a universal mapping algorithm for nucleotide base-conversion sequencingNucleic Acids ResearchJanuary 11, 2025Moping Xu, Xiaoyang Liu, Miao Wang, Tingting Luo, Yawei Gao, Jun Liu, Jiejun Shiisolated from HEK293T cells was firstly fragmented to ∼150-nt by magnesium RNA fragmentation buffer (NEB, E6150S) for 4 min at 94°C and purified by ethanol precipitation. To 5 μg of polyA + fragmented RNA (CMC-) in

(56) Optimized chemical labeling method for isolation of 8-oxoG-modified RNA, ChLoRox-Seq, identifies mRNAs enriched in oxidation and transcriptome-wide distribution biases of oxidation events post environmental stressRNA BiologyDecember 31, 2024Matthew R. Burroughs, Philip J. Sweet, Lydia M. ContrerasAfterwards, mRNA samples were fragmented using the ImageLab™ Magnesium RNA Fragmentation Module (NEB, E6150S) at 94°C for 4 minutes. Fragmented mRNA was re-purified using the Oligo Clean and Concentrator Kit (Zymo

(57) SERRATE drives phase separation behaviours to regulate m6A modification and miRNA biogenesisNature Cell BiologyDecember 1, 2024Songxiao Zhong, Xindi Li, Changhao Li, Haiyan Bai, Jingjing Chen, Lu Gan, Jiyun Zhu, Taerin Oh, Xingxing Yan, Jiaying Zhu, et al.with 10 fmol of methylated and unmethylated standards (NEB, E1610S) was used for fragmentation (NEB, E6150S). RNAs were then used for anti-m6A (NEB, E1610S) and anti-GFP (Sigma-Aldrich, G1546) IP, respectively.

(58) Identification of Long Non‐Coding RNA as Potential Biomarkers for the Diagnosis of Postmenopausal OsteoporosisInternational Journal of EndocrinologyNovember 23, 2024Zhu Yihua, Peng Chenjian, Wang Lining, Jiang Shujun, Yang Haomiao, Zhang Kaixuan, Dai Fenglei, Ma Yong, Chu Xudong, Zhang Chunlei, et al.ribo-minus RNA was fragmented into small pieces using Magnesium RNA Fragmentation Module (NEB, cat. e6150S, USA). Then, reversed transcription of the cleaved RNA fragments was performed with SuperScript™ II Reverse Transcriptase

(59) AKK-derived outer membrane vesicles alleviate indomethacin-induced mucin secretion reduction in LS174T cells by inhibiting endoplasmic reticulum stressFrontiers in Molecular BiosciencesNovember 7, 2024Lijun Zhang, Shuang Ma, Huixi Liang, Xin Chen, Jingwen ZhaomRNA was fragmented at high temperature using the NEBNextR Magnesium RNA Fragmentation Module (cat. E6150S, NEB, United States). CDNA synthesis from the fragmented RNA was carried out using Invitrogen SuperScriptTM

(60) The N6-methyladenosine Epitranscriptomic Landscape of Lung AdenocarcinomaCancer discoveryNovember 1, 2024Wang S, Zeng Y, Zhu L, Zhang M, Zhou L, Yang W, Luo W, Wang L, Liu Y, Zhu H, et al.Illumina). rRNA-depleted RNAs were fragmented using NEBNext Magnesium RNA Fragmentation Module (Cat. #E6150S, NEB) and then reverse-transcribed by SuperScript II Reverse Transcriptase (Cat. #18064071, Thermo Fisher Scientific

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NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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