NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(101) CRHR1 mediates the transcriptional expression of pituitary hormones and their receptors under hypoxiaFront Endocrinol (Lausanne)September 2, 2022Tong Ying Wang et al.Then the poly(A) RNA was fragmented into small pieces using the Mg2+ RNA fragmentation module (Cat. E6150S, NEB, USA) at 94°C for 5–7 min. Then the cleaved RNA fragments were reverse-transcribed to synthesize

(102) Socializing Models During Lactation Alter Colonic Mucosal Gene Expression and Fecal Microbiota of Growing PigletsFrontiers in MicrobiologyJuly 7, 2022Yanju Bi, Haidong Wei, Haoyang Nian, Runze Liu, Wenbo Ji, Honggui Liu, Jun Baocaptured mRNA was fragmented into small pieces using a Magnesium RNA Fragmentation Module (NEB cat.e6150S, USA) at 94°C for 5–7 min, and the cleaved RNA fragments were then reverse-transcribed to create the

(103) m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translationCell discoveryMay 21, 2022Zhuo W, Sun M, Wang K, Zhang L, Li K, Yi D, Li M, Sun Q, Ma X, Liu W, et al.overnight. Three μg total RNA was fragmented to ~150 nt using magnesium RNA fragmentation buffer (NEB, E6150S), and ethanol precipitated. The fragmented RNA was denatured at 65 °C for 5 min and chilled on ice immediately,

(104) Laser Microdissection for Species-Agnostic Single-Tissue ApplicationsJournal of Visualized ExperimentsApril 18, 2022Alyssa Woronik, Karin Kiontke, Raya S. Jallad, R. Antonio Herrera, David H. A. FitchGCCTTGGCACCCGAGAATTCCANNNNNN,IDT,,a primer with 6 nucleotides that are random RNA Fragmentation buffer,NEB,E6150S, RNA Fragmentation stop buffer,NEB,E6150S, RNA PCR Index Primers (RPI1–RPI48),"Illumina, NEB, or IDT",,"RPIX

(105) Loss of Slc38a4 imprinting is a major cause of mouse placenta hyperplasia in somatic cell nuclear transferred embryos at late gestationCell reportsFebruary 22, 2022Xie Z, Zhang W, Zhang Y.RNase-free",ThermoFisher Scientific,Cat#EN0521 NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,Cat#E6150S Second Strand Buffer,ThermoFisher Scientific,Cat#10812014 Magnesium Chloride (MgCl2),Sigma-Aldrich,Cat#M1028 Dithiothreitol (DTT

(106) Profiling Selective Packaging of Host RNA and Viral RNA Modification in SARS-CoV-2 Viral PreparationsFrontiers in Cell and Developmental BiologyFebruary 3, 2022Noah Peña et al.fragmentation experiment. Eluted RNA was then transferred to PCR tubes, 2 µl RNA fragmentation buffer (E6150S, NEB) was added to each tube and mixed well. Samples were incubated at 95°C on PCR machine for 6 min

(107) m6Am-seq reveals the dynamic m6Am methylation in the human transcriptomeNature CommunicationsDecember 1, 2021Hanxiao Sun, Kai Li, Xiaoting Zhang, Jun’e Liu, Meiling Zhang, Haowei Meng, Chengqi Yi100 μg >200 nt RNA was fragmented into ~150 nt-long fragments using RNA fragmentation buffer (NEB, E6150S). Adding fragment stop solution buffer stopped the reaction, and fragments were purified and concentrated

(108) The spatial RNA integrity number assay for in situ evaluation of transcriptome qualityCommunications BiologyDecember 1, 2021Linda Kvastad et al.was performed using NEBNext Magnesium RNA Fragmentation Module according to provided instructions (E6150S, New England Biolabs, NEB), at 94 °C for 10, 30 s, 2 or 4 min. Reverse transcription in solution for

(109) Modifying the m(6)A brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic taggingMolecular psychiatryDecember 1, 2021Martinez De La Cruz B, Markus R, Malla S, Haig MI, Gell C, Sang F, Bellows E, Sherif MA, McLean D, Lourdusamy A, et al.(Illumina, MRZG12324). Ribo-depleted RNA was fragmented to 100 bp using NebNext Fragmentation module (NEB, E6150S). 1/10 volume of fragmented RNA was separated to use as input control. 5 μg of m6A Ab (abcam, ab151230)

(110) Changes in Transcriptomic Profiles in Different Reproductive Periods in YaksBiology (Basel)November 25, 2021Shaoke Guo et al.All captured mRNA was fragmented using the NEBNext® Magnesium RNA Fragmentation Module [Catalog No. E6150S, New England BioLabs (NEB)™, Ipswich, MA, USA] with high-temperature conditions, at 86 °C for seven

(111) SPT5 stabilization of promoter-proximal RNA polymerase IIMolecular cellNovember 4, 2021Aoi Y, Takahashi YH, Shah AP, Iwanaszko M, Rendleman EJ, Khan NH, Cho BK, Goo YA, Ganesan S, Kelleher NL, et al.by the ChIP-seq and PRO-seq. Total RNA was fragmented using Magnesium RNA Fragmentation Module (NEB #E6150S), followed by biotinylation of 4sU using Biotin-XX-MTSEA (Biotium #90066) in 20% DMF. Biotinylated RNA

(112) Deep (Meta)genomics and (Meta)transcriptome Analyses of Fungal and Bacteria Consortia From Aircraft Tanks and Kerosene Identify Key Genes in Fuel and Tank CorrosionFrontiers in MicrobiologyOctober 1, 2021Ines Krohn et al.were fragmented with Mg2 + at 94°C for 3 min using the NEBNext Magnesium RNA Fragmentation Module (E6150S, New England Biolabs, Frankfurt am Main, Germany) followed by RNA purification with the Zymo RNA Clean

(113) Alternative 3' UTRs play a widespread role in translation-independent mRNA association with the endoplasmic reticulumCell reportsJuly 20, 2021Cheng LC, Zheng D, Zhang Q, Guvenek A, Cheng H, Tian B.Qubit dsDNA HS Assay Kit,Thermo Fisher Scientific,Q32854 NEBNext® Magnesium RNA Fragmentation Module,NEB,E6150S DC Protein Assay Reagents Package,Bio-Rad,5000116 DIG Northern Starter Kit,Roche,12039672910 Deposited

(114) Enhanced antioxidant capacity prevents epitranscriptomic and cardiac alterations in adult offspring gestationally-exposed to ENMNanotoxicologyJuly 3, 2021Amina Kunovac, Quincy A. Hathaway, Mark V. Pinti, Andrya J. Durr, Andrew D. Taylor, William T. Goldsmith, Krista L. Garner, Timothy R. Nurkiewicz, John M. Hollandercarried out, the RNA samples were fragmented using the NEBNext Magnesium RNA Fragmentation kit (item no. E6150s; New England Biolabs) (Zeng et al. 2018; Barros-Silva et al. 2020). To determine the necessary length

(115) YTHDF1 Promotes Cyclin B1 Translation through m⁶A Modulation and Contributes to the Poor Prognosis of Lung Adenocarcinoma with KRAS/TP53 Co-MutationCellsJuly 2, 2021Lou X, Ning J, Liu W, Li K, Qian B, Xu D, Wu Y, Zhang D, Cui W.indicated was fragmented into ~100 nt sections using the NEBNext Magnesium RNA Fragmentation Module (NEB E6150S). RNA was incubated with the m6A, YTHDF1, or IgG antibody (5 μg) for immunoprecipitation overnight at

(116) The m⁶A methylome of SARS-CoV-2 in host cellsCell researchApril 1, 2021Liu J, Xu YP, Li K, Ye Q, Zhou HY, Sun H, Li X, Yu L, Deng YQ, Li RT, et al.RNA) was fragmented into ~150-nucleotide-long fragments by magnesium RNA fragmentation buffer (NEB, E6150S). The fragmentation was stopped by RNA fragmentation stop solution followed by ethanol precipitation.

(117) VELCRO-IP RNA-seq reveals ribosome expansion segment function in translation genome-wideCell reportsJanuary 19, 2021Leppek K, Byeon GW, Fujii K, Barna M.fragments by magnesium-buffer based degradation using the NEBNext Magnesium RNA Fragmentation Module (NEB, E6150S). Fragmentation was optimized for time and RNA input amount monitoring RNA size using the mRNA Pico

(118) Maximizing transcription of nucleic acids with efficient T7 promotersCommunications BiologyDecember 1, 2020Thomas Conrad, Izabela Plumbom, Maria Alcobendas, Ramon Vidal, Sascha Sauerthe MEGAscript T7 kit. After EXOSAP-IT treatment (Thermo, 78201) and fragmentation for 1.5 min (NEB E6150S), RNA was cleaned up by 1.8 volumes of Ampure XP. The fragmented RNA was combined with HEX-primer and

(119) EndoVIPER‐seq for Improved Detection of A‐to‐I Editing Sites in Cellular RNACurrent Protocols in Chemical BiologyJune 1, 2020Steve D. Knutson, Jennifer M. HeemstraAM9780) Nuclease-free water NEBNext® Magnesium RNA Fragmentation Module (New England Biolabs, cat. No. E6150S) Sodium acetate anhydrous, molecular biology grade, ≥99% (Sigma Aldrich, cat. No. S2889) Acetic acid,

(120) G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress GranulesCellApril 16, 2020Yang P, Mathieu C, Kolaitis RM, Zhang P, Messing J, Yurtsever U, Yang Z, Wu J, Li Y, Pan Q, et al.RNA Cleanup Kit,New England Biolabs,T2040S NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,E6150S SuperScript III First-Strand Synthesis System,Thermo Fisher Scientific,18080051 Magnetic mRNA Isolation Kit

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NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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