NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(101) Loss of Slc38a4 imprinting is a major cause of mouse placenta hyperplasia in somatic cell nuclear transferred embryos at late gestationCell reportsFebruary 22, 2022Xie Z, Zhang W, Zhang Y.RNase-free",ThermoFisher Scientific,Cat#EN0521 NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,Cat#E6150S Second Strand Buffer,ThermoFisher Scientific,Cat#10812014 Magnesium Chloride (MgCl2),Sigma-Aldrich,Cat#M1028 Dithiothreitol (DTT

(102) Profiling Selective Packaging of Host RNA and Viral RNA Modification in SARS-CoV-2 Viral PreparationsFrontiers in Cell and Developmental BiologyFebruary 3, 2022Noah Peña et al.fragmentation experiment. Eluted RNA was then transferred to PCR tubes, 2 µl RNA fragmentation buffer (E6150S, NEB) was added to each tube and mixed well. Samples were incubated at 95°C on PCR machine for 6 min

(103) The spatial RNA integrity number assay for in situ evaluation of transcriptome qualityCommunications BiologyDecember 1, 2021Linda Kvastad et al.was performed using NEBNext Magnesium RNA Fragmentation Module according to provided instructions (E6150S, New England Biolabs, NEB), at 94 °C for 10, 30 s, 2 or 4 min. Reverse transcription in solution for

(104) m6Am-seq reveals the dynamic m6Am methylation in the human transcriptomeNature CommunicationsDecember 1, 2021Hanxiao Sun, Kai Li, Xiaoting Zhang, Jun’e Liu, Meiling Zhang, Haowei Meng, Chengqi Yi100 μg >200 nt RNA was fragmented into ~150 nt-long fragments using RNA fragmentation buffer (NEB, E6150S). Adding fragment stop solution buffer stopped the reaction, and fragments were purified and concentrated

(105) Modifying the m(6)A brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic taggingMolecular psychiatryDecember 1, 2021Martinez De La Cruz B, Markus R, Malla S, Haig MI, Gell C, Sang F, Bellows E, Sherif MA, McLean D, Lourdusamy A, et al.(Illumina, MRZG12324). Ribo-depleted RNA was fragmented to 100 bp using NebNext Fragmentation module (NEB, E6150S). 1/10 volume of fragmented RNA was separated to use as input control. 5 μg of m6A Ab (abcam, ab151230)

(106) Changes in Transcriptomic Profiles in Different Reproductive Periods in YaksBiology (Basel)November 25, 2021Shaoke Guo et al.All captured mRNA was fragmented using the NEBNext® Magnesium RNA Fragmentation Module [Catalog No. E6150S, New England BioLabs (NEB)™, Ipswich, MA, USA] with high-temperature conditions, at 86 °C for seven

(107) SPT5 stabilization of promoter-proximal RNA polymerase IIMolecular cellNovember 4, 2021Aoi Y, Takahashi YH, Shah AP, Iwanaszko M, Rendleman EJ, Khan NH, Cho BK, Goo YA, Ganesan S, Kelleher NL, et al.by the ChIP-seq and PRO-seq. Total RNA was fragmented using Magnesium RNA Fragmentation Module (NEB #E6150S), followed by biotinylation of 4sU using Biotin-XX-MTSEA (Biotium #90066) in 20% DMF. Biotinylated RNA

(108) Deep (Meta)genomics and (Meta)transcriptome Analyses of Fungal and Bacteria Consortia From Aircraft Tanks and Kerosene Identify Key Genes in Fuel and Tank CorrosionFrontiers in MicrobiologyOctober 1, 2021Ines Krohn et al.were fragmented with Mg2 + at 94°C for 3 min using the NEBNext Magnesium RNA Fragmentation Module (E6150S, New England Biolabs, Frankfurt am Main, Germany) followed by RNA purification with the Zymo RNA Clean

(109) Alternative 3' UTRs play a widespread role in translation-independent mRNA association with the endoplasmic reticulumCell reportsJuly 20, 2021Cheng LC, Zheng D, Zhang Q, Guvenek A, Cheng H, Tian B.Qubit dsDNA HS Assay Kit,Thermo Fisher Scientific,Q32854 NEBNext® Magnesium RNA Fragmentation Module,NEB,E6150S DC Protein Assay Reagents Package,Bio-Rad,5000116 DIG Northern Starter Kit,Roche,12039672910 Deposited

(110) Enhanced antioxidant capacity prevents epitranscriptomic and cardiac alterations in adult offspring gestationally-exposed to ENMNanotoxicologyJuly 3, 2021Amina Kunovac, Quincy A. Hathaway, Mark V. Pinti, Andrya J. Durr, Andrew D. Taylor, William T. Goldsmith, Krista L. Garner, Timothy R. Nurkiewicz, John M. Hollandercarried out, the RNA samples were fragmented using the NEBNext Magnesium RNA Fragmentation kit (item no. E6150s; New England Biolabs) (Zeng et al. 2018; Barros-Silva et al. 2020). To determine the necessary length

(111) YTHDF1 Promotes Cyclin B1 Translation through m⁶A Modulation and Contributes to the Poor Prognosis of Lung Adenocarcinoma with KRAS/TP53 Co-MutationCellsJuly 2, 2021Lou X, Ning J, Liu W, Li K, Qian B, Xu D, Wu Y, Zhang D, Cui W.indicated was fragmented into ~100 nt sections using the NEBNext Magnesium RNA Fragmentation Module (NEB E6150S). RNA was incubated with the m6A, YTHDF1, or IgG antibody (5 μg) for immunoprecipitation overnight at

(112) The m⁶A methylome of SARS-CoV-2 in host cellsCell researchApril 1, 2021Liu J, Xu YP, Li K, Ye Q, Zhou HY, Sun H, Li X, Yu L, Deng YQ, Li RT, et al.RNA) was fragmented into ~150-nucleotide-long fragments by magnesium RNA fragmentation buffer (NEB, E6150S). The fragmentation was stopped by RNA fragmentation stop solution followed by ethanol precipitation.

(113) VELCRO-IP RNA-seq reveals ribosome expansion segment function in translation genome-wideCell reportsJanuary 19, 2021Leppek K, Byeon GW, Fujii K, Barna M.fragments by magnesium-buffer based degradation using the NEBNext Magnesium RNA Fragmentation Module (NEB, E6150S). Fragmentation was optimized for time and RNA input amount monitoring RNA size using the mRNA Pico

(114) Maximizing transcription of nucleic acids with efficient T7 promotersCommunications BiologyDecember 1, 2020Thomas Conrad, Izabela Plumbom, Maria Alcobendas, Ramon Vidal, Sascha Sauerthe MEGAscript T7 kit. After EXOSAP-IT treatment (Thermo, 78201) and fragmentation for 1.5 min (NEB E6150S), RNA was cleaned up by 1.8 volumes of Ampure XP. The fragmented RNA was combined with HEX-primer and

(115) EndoVIPER‐seq for Improved Detection of A‐to‐I Editing Sites in Cellular RNACurrent Protocols in Chemical BiologyJune 1, 2020Steve D. Knutson, Jennifer M. HeemstraAM9780) Nuclease-free water NEBNext® Magnesium RNA Fragmentation Module (New England Biolabs, cat. No. E6150S) Sodium acetate anhydrous, molecular biology grade, ≥99% (Sigma Aldrich, cat. No. S2889) Acetic acid,

(116) G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress GranulesCellApril 16, 2020Yang P, Mathieu C, Kolaitis RM, Zhang P, Messing J, Yurtsever U, Yang Z, Wu J, Li Y, Pan Q, et al.RNA Cleanup Kit,New England Biolabs,T2040S NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,E6150S SuperScript III First-Strand Synthesis System,Thermo Fisher Scientific,18080051 Magnetic mRNA Isolation Kit

(117) Chromatin Hyperacetylation Impacts Chromosome Folding by Forming a Nuclear SubcompartmentMolecular cellApril 2, 2020Rosencrance CD, Ammouri HN, Yu Q, Ge T, Rendleman EJ, Marshall SA, Eagen KP.corresponding to 10 million cells. 2 μL 10x NEBNext RNA Fragmentation Buffer (New England Biolabs #E6150S) was added to 18 μL of sample (no more than 50 μg RNA, if more than 50 μg of RNA all volumes were linearly

(118) Transcriptome-wide dynamics of extensive m6A mRNA methylation during Plasmodium falciparum blood-stage developmentNature MicrobiologyDecember 1, 2019Sebastian Baumgarten, Jessica M. Bryant, Ameya Sinha, Thibaud Reyser, Peter R. Preiser, Peter C. Dedon, Artur ScherfmRNA was fragmented for 15 min at 70 °C to ~ 100 nt using the NEBNext fragmentation module (NEB no. E6150S) and purified using the Qiagen MinElute kit (Qiagen no. 74204) with slight modifications: to also purify

(119) BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencingGenome BiologyDecember 1, 2019Daniel Alpern, Vincent Gardeux, Julie Russeil, Bastien Mangeat, Antonio C. A. Meireles-Filho, Romane Breysse, David Hacker, Bart Deplancke2: Table S4). RNA fragmentation was done using the NEBNext Magnesium RNA Fragmentation Module (NEB, #E6150S) with incubation time at 94 °C for 1 or 2 min. This resulted in RNA with a variable extent of degradation

(120) Brain somatic mutations in MTOR reveal translational dysregulations underlying intractable focal epilepsyThe Journal of clinical investigationOctober 1, 2019Kim JK, Cho J, Kim SH, Kang HC, Kim DS, Kim VN, Lee JH.via alkaline hydrolysis using the NEBNext Magnesium RNA Fragmentation Module (New England BioLabs, E6150S). Ribosome footprints (~30 nt in size) and RNA fragments (40–60 nt in size) were size-fractionated by

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NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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