NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(61) SERRATE drives phase separation behaviours to regulate m6A modification and miRNA biogenesisNature Cell BiologyDecember 1, 2024Songxiao Zhong, Xindi Li, Changhao Li, Haiyan Bai, Jingjing Chen, Lu Gan, Jiyun Zhu, Taerin Oh, Xingxing Yan, Jiaying Zhu, et al.with 10 fmol of methylated and unmethylated standards (NEB, E1610S) was used for fragmentation (NEB, E6150S). RNAs were then used for anti-m6A (NEB, E1610S) and anti-GFP (Sigma-Aldrich, G1546) IP, respectively.

(62) Identification of Long Non‐Coding RNA as Potential Biomarkers for the Diagnosis of Postmenopausal OsteoporosisInternational Journal of EndocrinologyNovember 23, 2024Zhu Yihua, Peng Chenjian, Wang Lining, Jiang Shujun, Yang Haomiao, Zhang Kaixuan, Dai Fenglei, Ma Yong, Chu Xudong, Zhang Chunlei, et al.ribo-minus RNA was fragmented into small pieces using Magnesium RNA Fragmentation Module (NEB, cat. e6150S, USA). Then, reversed transcription of the cleaved RNA fragments was performed with SuperScript™ II Reverse Transcriptase

(63) AKK-derived outer membrane vesicles alleviate indomethacin-induced mucin secretion reduction in LS174T cells by inhibiting endoplasmic reticulum stressFrontiers in Molecular BiosciencesNovember 7, 2024Lijun Zhang, Shuang Ma, Huixi Liang, Xin Chen, Jingwen ZhaomRNA was fragmented at high temperature using the NEBNextR Magnesium RNA Fragmentation Module (cat. E6150S, NEB, United States). CDNA synthesis from the fragmented RNA was carried out using Invitrogen SuperScriptTM

(64) The N6-methyladenosine Epitranscriptomic Landscape of Lung AdenocarcinomaCancer discoveryNovember 1, 2024Wang S, Zeng Y, Zhu L, Zhang M, Zhou L, Yang W, Luo W, Wang L, Liu Y, Zhu H, et al.Illumina). rRNA-depleted RNAs were fragmented using NEBNext Magnesium RNA Fragmentation Module (Cat. #E6150S, NEB) and then reverse-transcribed by SuperScript II Reverse Transcriptase (Cat. #18064071, Thermo Fisher Scientific

(65) Transcriptomic and metabolomic analysis based on different aggressive pecking phenotype in duckScientific ReportsSeptember 27, 2024Baoguo Zhu, Jinjin Zhu, Ai Liu, Bingnong Yao, Fuyou Liao, Shenglin Yangpurification, the mRNA was fragmented into short fragments using a Magnesium RNA Fragmentation Module (E6150S, NEB, USA). Then the short RNA fragments were reverse-transcribed to cDNA with SuperScript™ II Reverse Transcriptase

(66) Protocol for transcriptome-wide mapping of small-molecule RNA-binding sites in live cellsSTAR ProtocolsSeptember 20, 2024Yuquan Tong, Patrick R.A. Zanon, Xueyi Yang, Xiaoxuan Su, Jessica L. Childs-Disney, Matthew D. Disneyfragmented to ∼150 nt lengths by using an NEBNext Magnesium RNA fragmentation module (New England Biolabs, E6150S). The minimum amount of total RNA used as input in this step is 5 μg, although 10 μg or more is recommended.

(67) Crosstalk between epitranscriptomic and epigenomic modifications and its implication in human diseasesCell GenomicsAugust 14, 2024Chengyu Li, Kexuan Chen, Qianchen Fang, Shaohui Shi, Jiuhong Nan, Jialin He, Yafei Yin, Xiaoyu Li, Jingyun Li, Lei Hou, et al.from that was fragmented to 200 nucleotide fragments by NEBNext Magnesium RNA Fragmentation Module (E6150S, NEB) at 94°C for 5 min and purified with RNA Clean & Concentrator-5 kit (R1016, Zymo Research). 1/10

(68) Enhanced ac4C detection in RNA via chemical reduction and cDNA synthesis with modified dNTPsRNAJuly 1, 2024Sebastien Relier, Sarah Schiffers, Hamid Beiki, Shalini Oberdoerfferusing NaBH4 as described previously. Untreated samples were fragmented using NEB fragmentation buffer (E6150S) for 3 min at 94°C. Fragmentation was stopped by adding NEB fragmentation stop solution and RNA was

(69) Phenethyl isothiocyanate inhibits the carcinogenic properties of hepatocellular carcinoma Huh7.5.1 cells by activating MAPK/PI3K-Akt/p53 signaling pathwaysPeerJJune 10, 2024Jiao Du, Yuting Zhang, Jiajia Chen, Libo Jin, Liying Pan, Pengyu Lei, Sue Linthe input for preparing libraries with the NEBNext® Magnesium RNA Fragmentation Module (catalog no. E6150S; NEB, Ipswich, MA, USA). Subsequently, Illumina NovaseqTM 6000 was used by LC-Bio Technology Co., Ltd.

(70) Redox regulation of m(6)A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetesNature cell biologyMarch 1, 2024De Jesus DF, Zhang Z, Brown NK, Li X, Xiao L, Hu J, Gaffrey MJ, Fogarty G, Kahraman S, Wei J, et al.described above and 50μg were fractionated using NEBNext Magnesium RNA Fragmentation Module (NEB, # E6150S). 18μl of total RNA were used for m6A-immunoprecipitation (m6A-IP) using the EpiMark N6-Methyladenosine enrichment kit

(71) Cell type-specific regulation of m(6) A modified RNAs in the aging Drosophila brainAging cellMarch 1, 2024Perlegos AE, Byrns CN, Bonini NM.NEBNext Poly(A) mRNA Magnetic Isolation Module. PolyA+ RNA was fragmented using the NEB Next Magnesium Fragmentation Module (NEB, E6150S) for 4 min at 95°C for a 250 ng sample of polyA+ RNA, and RNA was repurified using the Zymo RNA clean

(72) N 6 -methyladenosine (M 6 A) in fetal offspring modifies mitochondrial gene expression following gestational nano-TiO 2 inhalation exposureNanotoxicologyJanuary 18, 2024Amina Kunovac, Quincy A. Hathaway, Dharendra Thapa, Andrya J. Durr, Andrew D. Taylor, Saira Rizwan, Daud Sharif, Stephen J. Valentine, John M. Hollanderfragmented into ~250-350-nucleotide fragments using the NEBNext Magnesium RNA Fragmentation kit (item no. E6150S; New England Biolabs (NEB), Ipswich, MA) at 65°C for 5 minutes, followed by addition of the stop solution

(73) A multidimensional platform of patient-derived tumors identifies drug susceptibilities for clinical lenvatinib resistanceActa pharmaceutica Sinica. BJanuary 1, 2024Sun L, Wan AH, Yan S, Liu R, Li J, Zhou Z, Wu R, Chen D, Bu X, Ou J, et al.using a NEBNextR Magnesium RNA Fragmentation Module kit (NEBNextR Magnesium RNA Fragmentation Module (E6150S, NEB, USA) at 94 °C for 5–7 min. The fragmented RNA was performed PCR amplification with sequencing

(74) Expression and Prognostic Value of m6A RNA Methylation-Related Genes in Thyroid CancerIranian Journal of Public HealthSeptember 9, 2023Hui Han, Xiaodan Fu, Yu Zhang, Dingcun Luo, Xianfeng Zhang, Xiaohong WuDynabeads Oligo (dT) (25-61005, Thermo Fisher) was fragmented by Magnesium RNA Fragmentation Module (E6150S, NEB, USA) with the conditions of 86°C for 7 minutes. Fragmented RNA, immuno-magnetic beads (14311D,

(75) Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on DrosophilaSTAR ProtocolsSeptember 7, 2023J. Eduardo Pérez-Mojica, Lennart Enders, Kin H. Lau, Adelheid Lempradl,Thermo Fisher Scientific,Cat# AM1333 NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,Cat# E6150S Agilent RNA 6000 Pico Kit,Agilent Technologies,Cat# 5067-1513 Qubit dsDNA HS (High Sensitivity) Assay

(76) DOT1L activity affects neural stem cell division mode and reduces differentiation and ASNS expressionEMBO reportsAugust 3, 2023Appiah B, Fullio CL, Ossola C, Bertani I, Restelli E, Cheffer A, Polenghi M, Haffner C, Garcia-Miralles M, Zeis P, et al.For PCR Product Clean‐Up,Affymetrix,Cat# 78200 NEBNext Magnesium RNA Fragmentation Module,NEB,Cat# E6150S "randomhexRT primer, GCCTTGGCACCCGAGAATTCCANNNNNN",Integrated DNA technologies,Sagar et al (2018) "RNA

(77) TET2-mediated mRNA demethylation regulates leukemia stem cell homing and self-renewalCell stem cellAugust 3, 2023Li Y, Xue M, Deng X, Dong L, Nguyen LXT, Ren L, Han L, Li C, Xue J, Zhao Z, et al.μg mRNA was fragmented into 100–200 nt in length using NEBNext® Magnesium RNA Fragmentation Module (E6150S, New England Biolabs). The fragmented RNA was purified and 5 μg mRNA was incubated with 5 μg anti-m5C

(78) Teosinte Pollen Drive guides maize domestication and evolution by RNAibioRxivAugust 3, 2023Benjamin Berube, Evan Ernst, Jonathan Cahn, Benjamin Roche, Cristiane de Santis Alves, Jason Lynn, Armin Scheben, Adam Siepel, Jeffrey Ross-Ibarra, Jerry Kermicle, et al.ligated RNA to ≤200nt was performed using the Magnesium RNA fragmentation kit (New England Biolabs, cat#E6150S). 2ul RNA Fragmentation Buffer was added and samples were incubated at 94°C for 5 minutes followed by

(79) QKI shuttles internal m(7)G-modified transcripts into stress granules and modulates mRNA metabolismCellJuly 20, 2023Zhao Z, Qing Y, Dong L, Han L, Wu D, Li Y, Li W, Xue J, Zhou K, Sun M, et al.polyA+ purification) was fragmented into 100 nt using NEBNext® Magnesium RNA Fragmentation Module (E6150S, New England Biolabs) following the manufacturer’s protocol. The fragmented mRNA was decapped using

(80) Unique Features of the m6A Methylome and Its Response to Salt Stress in the Roots of Sugar Beet (Beta vulgaris)International Journal of Molecular SciencesJuly 19, 2023Junliang Li, Qiuying Pang, Xiufeng YanUSA), and then, fragmented into short pieces using a NEBNext® Magnesium RNA Fragmentation Module (#E6150S, NEB, Ipswich, MA, USA) at 86 °C for 7 min. The cleaved RNA fragments were incubated at 4 ℃ for 2 h

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NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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