NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) SARS-CoV-2 and HCoV-OC43 regulate host m6A modification via activation of the mTORC1 signalling pathway to facilitate viral replicationEmerging microbes & infectionsDecember 1, 2025Zhou S, Hui X, Wang W, Zhao C, Jin M, Qin Y, Chen M.(Thermo Fisher). The mRNA was then fragmented using the NEBNext® Magnesium RNA Fragmentation Module (NEB, E6150S) at 86°C for 7 min according to the manufacturer’s instructions, with 10% of the mRNA saved as input.

(22) Targeting Fibrotic Scarring by Mechanoregulation of Il11ra1 + /Itga11 + Fibroblast Patterning Promotes Axon Growth after Spinal Cord InjuryAdvanced ScienceNovember 1, 2025Longyou Xiao, Kaixi Shi, Wen Li, Jialin Liu, Pengfei Xie, Zijun Hu, Yu Dai, Haiyan Weng, Qiuju Yuan, Wutian Wu, et al.Fragmentation was performed at 94 °C for 5–7 min using the NEBNext Magnesium RNA Fragmentation Module (E6150S, NEB, USA). First‐strand cDNA synthesis was performed using SuperScript II (1 896 649, Thermo Fisher

(23) Arsenic regulates ALKBH1 abundance and substrate specificity to promote translation and tumorigenicityCell ReportsOctober 28, 2025Emma Wilkinson, Yan-Hong Cui, Zizhao Yang, Michelle Verghese, Seungwon Yang, Ming Sun, Houxiang Zhu, Jack Peterson, Jiangbo Wei, Yingming Zhao, et al.61012). 1 μg of mRNA was fragmented to ∼200 nt using the NEBNext Magnesium RNA Fragmentation Module (NEB, E6150S) according to the manufacturer’s instructions. 5% of the fragmented mRNA was saved as input. mRNA fragments

(24) Prognostic relevance of PRSS2 and its immune correlates in papillary thyroid carcinomaOpen Med (Wars)October 23, 2025Wei Lin, Linwen Zeng, Xiaoxiao Jiang, Xiangdong Kong, Jianming Gong, Ming Wufollowed by fragmentation with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, E6150S). cDNA was generated from the fragmented RNA and amplified to construct sequencing libraries with an

(25) Concentration-dependent formation of intersegment interactions in the viral inclusions of influenza A virus infected cellsiScienceOctober 17, 2025Naoki Takizawa, Koichi Higashi, Risa Karakida Kawaguchi, Yasuhiro Gotoh, Tetsuya Hayashi, Ken Kurokawahydrochloride,Sigma-Aldrich,A4330-5MG ISOGEN II,NIPPON GENE,311-07361 NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,E6150S CIAP,Takara Bio,2250A T4 polynucleotide kinase,Toyobo,PNK-111 ReverTra Ace,Toyobo,TRT-101 Taq polymerase,Roche,11146165001 Dynabeads MyOne Streptavidin C1

(26) RNA m1A methyltransferase TRMT61A promotes colorectal tumorigenesis by enhancing ONECUT2 mRNA stability and is a potential therapeutic targetCancer CommunicationsOctober 16, 2025Xiaoting Zhang, Na Qin, Fenfen Ji, Hao Su, Haiyun Shang, Hongyan Chen, Dan Huang, Qing Li, Jing Ren, Weixin Liu, et al.fragmented into ∼200 bp pieces with the New England Biolabs Next® Magnesium RNA Fragmentation Module (#E6150S, New England Biolabs) and then purified with Ethanol precipitation. One‐tenth of purified RNAs were

(27) PINK1 overexpression suppresses p38 MAPK/NF‑κB signaling to attenuate chondrocyte senescence in osteoarthritisInt J Mol MedOctober 15, 2025Lishi Jie, Yuanhui Zhang, Jiangyu Liu, Houyu Fu, Zaishi Zhu, Zeling Huang, Xiaoqing Shi, Peimin Wang, Songjiang Yinfragmentation buffer with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, cat. no. E6150S). First-strand cDNA was synthesized using random hexamer primers (50 μM; Invitrogen, cat. no. N8080127)

(28) Integrated 16 S rRNA and transcriptome analysis reveal molecular and microbial mechanisms of cold-tolerant germination in hulless barleyBMC GenomicsOctober 10, 2025Panrong Ren, Jie Wang, Lei Gongwere fragmented at 94℃ for 5–7 min using the NEBNextR Magnesium RNA Fragmentation Module (NEB, cat. E6150S, USA). Subsequently, the fragmented RNA (1 µg, 50 ng/µL) underwent reverse transcription using the Invitrogen

(29) α-hederin inhibits cervical cancer progression by inducing DNA damage-dependent cell cycle blockade and apoptosisBMC cancerOctober 3, 2025Song Y, Qin H, Huang S, Wang J, Huang Y, Lin Z, Yang F, Zhang X, Wang R, Wang J.USA), with RNase H (NEB, No. m0209, USA), and RNase H (NEBNext® Magnesium RNA Fragmentation Module, No. E6150S, USA). RNase H (NEB, item no. m0297, USA) for two-strand synthesis to convert these composite double

(30) Coupled transcriptome and proteome analysis of L3 and L4 developmental stages of Anisakis simplex s. s.: insights into target genes under glucose influenceBMC GenomicsSeptember 29, 2025Iwona Polak, Robert Stryiński, Mateusz Maździarz, Lukasz Paukszto, Mónica Carrera, Iwona Bogacka, Elżbieta Łopieńska-Biernatmanufacturer’s instructions. After removal of the ribosomal RNAs, the remaining RNAs were fragmented (Cat. No. E6150S, NEBNext® Magnesium RNA Fragmentation Module, New England Biolabs, Ipswich, MA, USA) into short fragments

(31) Lysosomal RNA profiling reveals targeting of specific types of RNAs for degradationbioRxivSeptember 16, 2025G. Jordan Ray, Eleonora Nardini, Heather R. Keys, Daniel H. Lin, David M. Sabatini, David P. Bartelconcentrators kits as above. Then, RNA was fragmented for 7 min at 94°C using NEB RNA fragmentation module (E6150S), before again purifying RNA using Trizol LS and zymo-RNA clean and concentrators kits as above. Then,

(32) METTL3/ALKBH5‐Mediated N6‐Methyladenosine Modification Drives Macrophage M1 Polarization via the SLC15A3‐TASL‐IRF5 Signaling Axis in PsoriasisAdvanced ScienceSeptember 1, 2025Tao Huang, Shijun Chen, Ke Ding, Liyan Yuan, Weiqi Lv, Kechen Chen, Yuchen Liu, Dongzhao Ma, Xin Zhang, Xiaobo Wang, et al.eluted RNA was fragmented at 94°C for 30 seconds using RNA Fragmentation Reagents (New England Biolabs, E6150S) and purified using the ZYMO RNA Clean & Concentrator Kit (Zymo Research, R1016). Proceed with the m6A

(33) Injectable sustained-release danshensu sodium-loaded nanoparticle hydrogel targets macrophages to improve myocardial microenvironment for myocardial infarction treatmentBioact MaterAugust 17, 2025Jianqiu Yang, Jing Wang, Zhihua Zeng, Zhenyu Chen, Dingguo Wang, Yongbing Wu(NEB, No. m0209, USA) and RNase H (NEB, no. m0209; NEBNext® Magnesium RNA Fragmentation Module, No. E6150S, USA; and NEB, item no. m0297, USA) was used for second - strand synthesis, converting DNA - RNA hybrids

(34) Association of alterations in transcriptomics and intestinal immune responses with Bifidobacterium longum BAA2573 in improving dextran sulfate sodium-induced colitisBMC GastroenterolAugust 1, 2025Wujuan Hao, Lan Gu, Renmin Zhou, Cuilan Huang, Xuyang Wang, Yanshan Liu, Qiong Lincaptured mRNA was segmented at high temperatures (94 ℃, 6 min) using a magnesium ions interrupt kit (Cat.E6150S, NEB, USA). RNA fragments were synthesized into double-stranded cDNA using reverse transcriptase (Cat.1896649,

(35) Identification of NPR2 gene mutations affecting chondrocyte differentiation in short stature through JAK2-STAT5Orphanet J Rare DisAugust 1, 2025Shuoshuo Wei, Mingming He, Chuanpeng Zhang, Yanying Li, Mei Zhang, Xinguo Hou, Bo Ban, Qianqian ZhaoOligo (dT) (Thermo Fisher, USA) and fragmented using a magnesium-based fragmentation kit (NEB, cat.e6150s, USA) at 94 °C for 5–7 min. The fragmented mRNA fragments were then reverse transcribed using SuperScript™ II Reverse Transcriptase

(36) The catalytic efficiency of METTL16 affects cellular processes by governing the intracellular S-adenosylmethionine setpointCell reportsJuly 22, 2025Flaherty JN, Sivasudhan E, Tegowski M, Xing Z, McGinnis MM, Hunter OV, Featherston KM, Sethia K, Tu BP, Meyer KD, et al.assays ,, CellTiter-Glo Reagent,ProMega,Cat#G7570 NEBNext Magnesium RNA Fragmentation Module,NEB,Cat#E6150S RiboMinus Eukaryote System V2,ThermoFisher,A15026 NEBNext Small RNA Library Prep Set for Illumina,NEB,E7330S "NEBNext Multiplex Oligos for Illumina, Index primers set 1

(37) The PLEKHA1-TACC2 fusion gene drives tumorigenesis via vascular mimicry formation in esophageal squamous-cell carcinomaCell Death & DifferentiationJuly 5, 2025Ting Yang, Zhi-Rui Lin, Tian-Liang Xia, Shang-Xin Liu, Bo-Yu Yuan, Yi-Ling Luo, Wen-Ting Du, Chao-Bo Lei, Yong-Zhan Nie, Mu-Sheng Zeng, et al.(ThermoFisher; EN0521) and then randomly fragmented via fragmentation buffer (New England Biolabs; E6150S). The first and second cDNA strand were synthesized with random hexamer primers by using Superscript

(38) Stress granule assembly impairs macrophage efferocytosis to aggravate allergic rhinitis in miceNat CommunJuly 1, 2025Ye Zhou, Zixuan Yang, Yuanyuan Wang, Yue Dong, Tianyu Wang, Yunhui Li, Caiquan Liang, Yanfang Liu, Zhixuan Li, Shanrong Liu, et al.(ab104139) were purchased from Abcam (Cambridge, UK). NEB Next Magnesium RNA Fragmentation Module (E6150S) and T4 polynucleotide kinase (M0201S) were purchased from New England Biolabs (Ipswich, MA). Tobacco

(39) Small-molecule-catalysed deamination enables transcriptome-wide profiling of N6-methyladenosine in RNANature ChemistryJuly 1, 2025Pingluan Wang, Chang Ye, Michelle Zhao, Bochen Jiang, Chuan Heextracted from total RNA and 10–20 ng of the RNA was fragmented using RNA fragmentation reagents (NEB, E6150S) at 94 °C for 2 min, followed by purification with the RNA Clean and Concentrator kit (Zymo Research).

(40) N6-methyladenosine-modified GPX2 impacts cancer cell stemness and TKI resistance through regulating of redox metabolismCell Death DisJune 18, 2025Xu Yang, Long Yu, Miaomiao Shao, Huiling Yang, Kangwei Qi, Gaofei He, Lanxin Wang, Di Kong, Jianxin Gu, Xiaolin Xu, et al.15596026CN). RNA was randomly fragmented by using NEBNext® Magnesium RNA Fragmentation Modul (NEB, E6150S). Anti-IgG and anti-m6A (Cell Signaling Technology, 56593) antibodies were conjugated to Protein G-Agarose beads

Be the First to Write a Review!

NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

New England Biolabs

Citations (133)

Reviews

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map