ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(161) WNT5A-RHOA Signaling Is a Driver of Tumorigenesis and Represents a Therapeutically Actionable Vulnerability in Small Cell Lung CancerCancer researchNovember 15, 2022Kim KB, Kim DW, Kim Y, Tang J, Kirk N, Gan Y, Kim B, Fang B, Park JL, Zheng Y, et al.protocol. cDNA was generated by the ProtoScript II first strand cDNA synthesis kit (New England Biolabs, E6560). Quantitative PCR (RT-qPCR) was performed using SYBR Green (Thermo Fisher Scientific, 4367659) with

(162) Long-range phasing of dynamic, tissue-specific and allele-specific regulatory elementsNature GeneticsOctober 4, 2022Sofia Battaglia, Kevin Dong, Jingyi Wu, Zeyu Chen, Fadi J. Najm, Yuanyuan Zhang, Molly M. Moore, Vivian Hecht, Noam Shoresh, Bradley E. Bernstein740984.50). cDNA synthesis was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560) using the Random Primer Mix. RT-qPCR was performed using SYBR Select Master Mix (Applied Biosystems,

(163) Sphingosine-1-phosphate-lyase deficiency affects glucose metabolism in a way that abets oncogenesisMolecular oncologyOctober 1, 2022Afsar SY, Alam S, Fernandez Gonzalez C, van Echten-Deckert G.performed using the ProtoScript® II First Strand cDNA Synthesis kit (New England Biolabs, Ipswich, MA, USA; E6560L). The resulting total cDNA was then applied in real‐time PCR (CFX96‐real‐time PCR; Bio‐Rad Laboratories)

(164) COVID-19 Whole-Genome Resequencing with Redundant Tiling PCR and Subtract-Based Amplicon Normalization Successfully Characterized SARS-CoV-2 Variants in Clinical SpecimensInterdisciplinary Perspectives on Infectious DiseasesSeptember 28, 2022Tatsuki Sugi, Mizanur Rahman, Rummana Rahim, Abu Hasan, Naoko Kawai, Kyoko Hayashida, Junya Yamagishiinstructions. First-strand cDNA was synthesized using a ProtoScript II first-strand cDNA synthesis kit (E6560S, NEB) using oligo-dT and random 9-mer primers. An in vitro amplified virus isolate (EPI_ISL_408667)

(165) A high-throughput drug discovery pipeline to optimize kidney normothermic machine perfusionFrontiers in PhysiologySeptember 26, 2022Smilla Hofmann, Florian Grahammer, Ilka Edenhofer, Victor G. Puelles, Tobias B. Huber, Jan Czogallaused for DNA digestion. For reverse transcription, Protoscript II First Strand cDNA Synthesis Kit (E6560L, New England BioLabs) was applied. Quantitative PCR was performed on a QuantStudio 3 System (A28566,

(166) Genomic epidemiology identifies emergence and rapid transmission of SARS-CoV-2 B.1.1.7 in the United StatesmedRxivSeptember 14, 2022Nicole L. Washington et al.al., 2017) and reagents from the Protoscript First Strand cDNA Synthesis Kit (New England Biolabs, #E6560L) and the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs, #M0493L). Amplicons were

(167) The B.1.427/1.429 (epsilon) SARS-CoV-2 variants are more virulent than ancestral B.1 (614G) in Syrian hamstersbioRxivSeptember 8, 2022Timothy Carroll et al.first strand cDNA synthesis using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs #E6560) with random hexamers and following manufacturer’s instructions. PCR amplification was performed using

(168) Generation of full-length circular RNA libraries for Oxford Nanopore long-read sequencingPLOS ONESeptember 7, 2022Steffen Fuchs, Loélia Babin, Elissa Andraos, Chloé Bessiere, Semjon Willier, Johannes H. Schulte, Christine Gaspin, Fabienne Meggettoused to create cDNA with the ProtoScript II First Strand cDNA Synthesis kit (New England Biolabs, #E6560S) according to the manufacturer using random hexamer primers. An RT-PCR was performed using the FastStart

(169) Removal of a 10-kb Gret1 transposon from VvMybA1 of Vitis vinifera cv. ChardonnayHorticulture ResearchSeptember 6, 2022Yingzhen Yang, John Ke, Xiaoyan Han, Wegi A Wuddineh, Guo-qing Song, Gan-Yuan Zhong(RT) in 20 μl volume with d(T)23 VN primer using the Postscript II first strand cDNA synthesis kit (E6560, New England Biolabs). For each RT-PCR reaction, 0.5 μl RT reaction was used in a 20 μl-reation with

(170) Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processesPLoS pathogensSeptember 1, 2022Yang E, Huang S, Jami-Alahmadi Y, McInerney GM, Wohlschlegel JA, Li MMH.Scientific,20-300B, Commercial assay or kit,Protoscript II First Strand cDNA Synthesis Kit,New England Biolabs,E6560L, Commercial assay or kit,Q5 Site-Directed Mutagenesis Kit,New England Biolabs,E0552S, Commercial assay

(171) Engineered U1 snRNAs to modulate alternatively spliced exonsMethodsSeptember 1, 2022Samuel T. Hatch, Aaron A. Smargon, Gene W. Yeoand process to cDNA by mixing the determined mass of RNA (250–500 ng), 1 uL d(T)23VN primer (50 uM; E6560, NEB), 5 uL 2x ProtoScript II Reaction Mix (E6560, NEB), 1 uL 10x ProtoScript II Enzyme Mix (E6560,

(172) IL-37 expression reduces acute and chronic neuroinflammation and rescues cognitive impairment in an Alzheimerâ€™s disease mouse modeleLifeAugust 30, 2022Niklas Lonnemann, Shirin Hosseini, Melanie Ohm, Robert Geffers, Karsten Hiller, Charles A Dinarello, Martin KorteTriFast,Avantor,N/A, commercial assay or kit,ProtoScript II First Strand cDNA Synthesis Kit,New England Biolabs Inc.,Cat# E6560, "strain, strain background (Mus musculus)","B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax mice",The Jackson

(173) IL-37 expression reduces acute and chronic neuroinflammation and rescues cognitive impairment in an Alzheimer’s disease mouse modelElifeAugust 30, 2022Niklas Lonnemann, Shirin Hosseini, Melanie Ohm, Robert Geffers, Karsten Hiller, Charles A Dinarello, Martin KorteTriFast,Avantor,N/A, commercial assay or kit,ProtoScript II First Strand cDNA Synthesis Kit,New England Biolabs Inc.,Cat# E6560, "strain, strain background (Mus musculus)","B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax mice",The Jackson

(174) NODULIN HOMEOBOX is required for heterochromatin homeostasis in ArabidopsisNature CommunicationsAugust 27, 2022Zsolt Karányi et al.and U6- or PP2AA3 reference RNA specific primer according to the manufacturer’s instructions (NEB, E6560). First, the stem-loop RT primer was hybridized to the sRNA molecule and then reverse transcribed in

(175) The impact of thigmotaxis deprivation on the development of the German cockroach (Blattella germanica)iScienceAugust 19, 2022Yun-Ru Chen, De-Wei Li, Hsin-Ping Wang, Shih-Shun Lin, En-Cheng Yangbeads,New England BioLabs,S1419S ProtoScript® II First Strand cDNA Synthesis Kit,New England BioLabs,E6560S DNA polymerase I (E. coli),New England BioLabs,M0209S Rnase H,New England BioLabs,M0297S NEBNext® Ultra™ II End Repair/dA-Tailing Module

(176) Voltage-gated sodium channel scn8a is required for innervation and regeneration of amputated adult zebrafish finsProceedings of the National Academy of Sciences of the United States of AmericaJuly 12, 2022Osorio-Méndez D, Miller A, Begeman IJ, Kurth A, Hagle R, Rolph D, Dickson AL, Chen CH, Halloran M, Poss KD, et al.300 ng to 1 μg of total RNA using an NEB ProtoScript II first-strand cDNA synthesis kit (catalog no. E6560). For RT-PCR, HuC/elavl3 was used for the brain-specific marker; and4 was used for regenerating fin

(177) Expression pattern of Stomatin-domain proteins in the peripheral olfactory systemScientific reportsJuly 6, 2022Gonzalez-Velandia KY, Hernandez-Clavijo A, Menini A, Dibattista M, Pifferi S.DNA. cDNA was synthetized using the Protoscript II First-Strand DNA Synthesis Kit from 100 ng of mRNA (E6560S, New England Biolabs). PCR was performed in thermocycler (ThermaCycler2720, LifeTechnologies) using

(178) Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinomaInternational journal of oncologyJuly 1, 2022Huang X, Huang Y, Lv Z, Wang T, Feng H, Wang H, Du S, Wu S, Shen D, Wang C, et al.as isopropanol precipitation. Reverse transcription was then conducted using a cDNA synthesis kit (E6560S, New England Biolabs) at 42°C for 15 min. Subsequent quantitative polymerase chain reaction (qPCR) analyses

(179) Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerabilityNature chemical biologyJuly 1, 2022Li Z, Ferguson L, Deol KK, Roberts MA, Magtanong L, Hendricks JM, Mousa GA, Kilinc S, Schaefer K, Wells JA, et al.of RNA samples were reverse transcribed using Protoscript II First Strand cDNA Synthesis Kit (NEB #E6560S). cDNA was measured using a QuantStudio 7 Flex Real-time PCR machine (Applied Biosystems) with Power

(180) Vision-related convergent gene losses reveal SERPINE3â€™s unknown role in the eyeeLifeJune 21, 2022Henrike Indrischek, Juliane Hammer, Anja Machate, Nikolai Hecker, Bogdan Kirilenko, Juliana Roscito, Stefan Hans, Caren Norden, Michael Brand, Michael Hillerkit,Qiagen,"Cat. #: 75,144", commercial assay or kit,ProtoScript II First Strand Synthesis kit,NEB,Cat. #: E6560S, commercial assay or kit,TSA Plus Cy3/Cy5 kit,Perkin-Elmer,Cat. #: NEL744001KT, commercial assay or

Be the First to Write a Review!

ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

New England Biolabs

Citations (276)

Reviews

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map