ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(121) Integrative high-throughput enhancer surveying and functional verification divulges a YY2-condensed regulatory axis conferring risk for osteoporosisCell GenomicsMarch 13, 2024Xiao-Feng Chen, Yuan-Yuan Duan, Ying-Ying Jia, Qian-Hua Dong, Wei Shi, Yan Zhang, Shan-Shan Dong, Meng Li, Zhongbo Liu, Fei Chen, et al.Cat#T2030S, USA). Subsequently, first strand cDNA synthesis was performed using RT-UMI (Table S6) (NEB, Cat#E6560S, USA). Next, cDNA was treated with RNase A (Takara, Cat#2158, Japan) and purified with AMPure XP beads.

(122) FTO attenuates TNF-α-induced damage of proximal tubular epithelial cells in acute pancreatitis-induced acute kidney injury via targeting AQP3 in an N6-methyladenosine-dependent mannerRen FailMarch 6, 2024Xinghui Li, Qi Liang, Lu Liu, Shujun Chen, Yong Li, Yu Puextracted, and the concentration was tested. After cDNA synthesis, a commercial cDNA synthesis kit (E6560S, New England Biolabs, Inc., USA), CFX384 Touch Real-Time PCR Detection System (Bio-Rad, USA), and SYBR

(123) FTO attenuates TNF-α-induced damage of proximal tubular epithelial cells in acute pancreatitis-induced acute kidney injury via targeting AQP3 in an N6-methyladenosine-dependent mannerRen FailMarch 6, 2024Xinghui Li, Qi Liang, Lu Liu, Shujun Chen, Yong Li, Yu Puextracted, and the concentration was tested. After cDNA synthesis, a commercial cDNA synthesis kit (E6560S, New England Biolabs, Inc., USA), CFX384 Touch Real-Time PCR Detection System (Bio-Rad, USA), and SYBR

(124) Drosophila models of phosphatidylinositol glycan biosynthesis class A congenital disorder of glycosylation (PIGA-CDG) mirror patient phenotypesG3: Genes, Genomes, GeneticsMarch 6, 2024Holly J Thorpe, Katie G Owings, Miriam C Aziz, Madelyn Haller, Emily Coelho, Clement Y ChowRNA was used to synthesize cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB Cat #E6560L). Fifty nanogram of cDNA was used to perform quantitative PCR (qPCR) with PowerUp SYBR Green Master Mix

(125) Bi-allelic variants in CELSR3 are implicated in central nervous system and urinary tract anomaliesNPJ Genom MedMarch 1, 2024Jil D. Stegmann, Jeshurun C. Kalanithy, Gabriel C. Dworschak, Nina Ishorst, Enrico Mingardo, Filipa M. Lopes, Yee Mang Ho, Phillip Grote, Tobias T. Lindenberg, Öznur Yilmaz, et al.rt-PCR using ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs GmbH, Catalog No. E6560). cDNA Amplification of 2138 bp upstream of exon 1 to exon 2 transition of transcript celsr3-204 was

(126) A Drosophila screen identifies a role for histone methylation in ER stress preconditioningG3: Genes, Genomes, GeneticsFebruary 7, 2024Katie G Owings, Clement Y Chowstep. RNA was converted to cDNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix

(127) A fluid biomarker reveals loss of TDP-43 splicing repression in presymptomatic ALS–FTDNature MedicineFebruary 1, 2024Katherine E. Irwin, Pei Jasin, Kerstin E. Braunstein, Irika R. Sinha, Mark A. Garret, Kyra D. Bowden, Koping Chang, Juan C. Troncoso, Abhay Moghekar, Esther S. Oh, et al.Complementary DNA was derived from total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB, no. E6560S). Numerous primers were designed against cryptic exon targets and screened to identify primer pairs

(128) Harnessing non-Watson–Crick’s base pairing to enhance CRISPR effectors cleavage activities and enable gene editing in mammalian cellsProceedings of the National Academy of SciencesJanuary 9, 2024Shuliang Gao, Huiwen Guan, Hanan Bloomer, Douglas Wich, Donghui Song, Jennifer Khirallah, Zhongfeng Ye, Yu Zhao, Mengting Chen, Chutian Xu, et al.cDNA Synthesis. cDNA synthesis was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560) with 200 ng ATP-mRNA or 600 ng ZTP-mRNA in a total reaction volume of 20 µL. Reactions were incubated

(129) Diversification of small RNA pathways underlies germline RNA interference incompetence in wild Caenorhabditis elegans strainsGENETICSJanuary 3, 2024Han Ting Chou, Francisco Valencia, Jacqueline C Alexander, Avery Davis Bell, Diptodip Deb, Daniel A Pollard, Annalise B PaabyScientific), and cDNA was synthesized using the ProtoScript II First Stand cDNA Synthesis Kit (NEB #E6560S). The experiment was replicated as follows: from each experimental condition, we collected 2 RNA samples,

(130) A robust knock-in approach using a minimal promoter and a minicircleDevelopmental BiologyJanuary 1, 2024Margaret Keating, Ryan Hagle, Daniel Osorio-Méndez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kangsynthesized from 300 ng to 1 μg of total RNA using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). The sequences of the primers used for RT-PCR are listed in Table S2. Imaging, immunostaining, and

(131) Therapeutic potential of archaeal unfoldase PANet and the gateless T20S proteasome in P23H rhodopsin retinitis pigmentosa micePloS oneJanuary 1, 2024Brooks C, Kolson D, Sechrest E, Chuah J, Schupp J, Billington N, Deng WT, Smith D, Sokolov M.the RNeasy Mini Kit (74104, Qiagen) and reverse transcribed into cDNA using the Protoscript II Kit (E6560S, New England Biolabs). Samples were analyzed by qPCR using Brilliant II SYBR Green qPCR Master Mix (600828,

(132) leptin b and its regeneration enhancer illustrate the regenerative features of zebrafish heartsDevelopmental dynamics : an official publication of the American Association of AnatomistsJanuary 1, 2024Shin K, Begeman IJ, Cao J, Kang J.Complementary DNA (cDNA) was synthesized using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). Quantitative PCR was performed using the qPCRBIO SyGreen Blue Mix Separate-ROX (Genesee Scientific,

(133) Novel strains of Culex flavivirus and Hubei chryso-like virus 1 from the Anopheles mosquito in western KenyaVirus ResearchJanuary 1, 2024Olivia Wesula Lwande, Jonas Näslund, Andreas Sjödin, Rebecca Lantto, Verah Nafula Luande, Göran Bucht, Clas Ahlm, Bernard Agwanda, Vincent Obanda, Magnus Evanderet al., 2021). The RNA was converted to cDNA using ProtoScript II First Strand cDNA Synthesis Kit (E6560S) and New England Biolab's Random Primer 6 (S1230S). The NEBNext Ultra II Non-Directional RNA Second

(134) Convergent insulin and TGF‐β signalling drives cancer cachexia by promoting aberrant fat body ECM accumulation in a Drosophila tumour modelEMBO reportsDecember 6, 2023Daniel Bakopoulos, Sofya Golenkina, Callum Dark, Elizabeth L Christie, Besaiz J Sánchez‐Sánchez, Brian M Stramer, Louise Y Chengreverse transcription of 0.5 μg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, #E6560S). Three independent biological replicates were prepared for each genotype. qPCR was performed with the

(135) Codon optimality modulates protein output by tuning translation initiationbioRxivDecember 1, 2023Elijah F Lyons, Lou C Devanneaux, Ryan Y Muller, Anna V Freitas, Zuriah A Meacham, Maria V McSharry, Van N Trinh, Anna J Rogers, Nicholas T Ingolia, Liana F Lareau37°C, then treated with DNase. IVT product was then reverse transcribed with a ProtoScript II kit (NEB, E6560S) according to manufacturer’s instructions, then treated with RNase A and RNase H. Purified product was

(136) Utilizing AAV-mediated LEAPER 2.0 for programmable RNA editing in non-human primates and nonsense mutation correction in humanized Hurler syndrome miceGenome BiologyOctober 23, 2023Zongyi Yi, Yanxia Zhao, Zexuan Yi, Yongjian Zhang, Gangbin Tang, Xiaoxue Zhang, Huixian Tang, Wei Zhang, Ying Zhao, Huayuan Xu, et al.manufacturer’s instructions. 1 μg total RNA was reverse transcribed with ProtoScript II First cDNA Synthesis (E6560L, NEB). 10 μL of ProtoScript II Reaction Mix was supplemented with 4 μL DMSO (A3672.0250, APPLICHEM),

(137) Tyrosyl-DNA phosphodiesterase 1 (TDP1) and SPRTN protease repair histone 3 and topoisomerase 1 DNA–protein crosslinks in vivoOpen BiologyOctober 4, 2023Ivan Anticevic, Cecile Otten, Luka Vinkovic, Luka Jukic, Marta Popovic(NEB, T2040L). For reverse transcription, the ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560L) was used. Total RNA from zebrafish or mouse tissues was added in a volume corresponding to 1000 ng

(138) A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathwayeLifeSeptember 4, 2023R Samuel Herron, Alexander K Kunisky, Jessica R Madden, Vivian I Anyaeche, May Z Maung, Hun-Way HwangBiolabs,Cat#C3040, Commercial assay or kit,ProtoScript II First Strand cDNA Synthesis Kit,New England Biolabs,Cat#E6560, Commercial assay or kit,Q5 Site-Directed Mutagenesis Kit,New England Biolabs,Cat#E0554, Commercial

(139) A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathwayElifeSeptember 4, 2023R Samuel Herron, Alexander K Kunisky, Jessica R Madden, Vivian I Anyaeche, May Z Maung, Hun-Way HwangBiolabs,Cat#C3040, Commercial assay or kit,ProtoScript II First Strand cDNA Synthesis Kit,New England Biolabs,Cat#E6560, Commercial assay or kit,Q5 Site-Directed Mutagenesis Kit,New England Biolabs,Cat#E0554, Commercial

(140) In Silico Bioinformatics Analysis on the Role of Long Non-Coding RNAs as Drivers and Gatekeepers of Androgen-Independent Prostate Cancer Using LNCaP and PC-3 CellsCurrent Issues in Molecular BiologySeptember 1, 2023Mandisa Mbeje, Jeyalakshmi Kandhavelu, Clement Penny, Mmamoletla Kgoebane-Maseko, Zodwa Dlamini, Rahaba MarimaProtoScript® II First Strand cDNA Synthesis Kit was used (New England Biolabs, Ipswich, MA, USA) (Catalogue number: E6560S). A Bio-Rad PCa-specific 384-well plate lncRNA PCR Array (Hercules, CA, USA) (Catalogue number: 12004237)

Be the First to Write a Review!

ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

New England Biolabs

Citations (285)

Reviews

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map