ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(201) Human Cytomegalovirus miR-UL70-3p Downregulates the H(2)O(2)-Induced Apoptosis by Targeting the Modulator of Apoptosis-1 (MOAP1)International journal of molecular sciencesDecember 21, 2021Pandeya A, Khalko RK, Mishra A, Singh N, Singh S, Saha S, Yadav S, Saxena S, Gosipatala SB.prepared using 1 µg of total RNA through the Proto Script® II First Strand cDNA Synthesis Kit (Cat no: E6560S; New England Biolabs, Rowley, MA, USA). qRT-PCR was carried out using SYBR green chemistry by using

(202) RNAmetasome network for macromolecule biogenesis in human cellsCommunications BiologyDecember 15, 2021Shiro Iuchi, Joao A. Pauloepitope We purified total RNA from HEK293T cultures, and synthesized cDNA in ProtoScript II mixture (NEB, E6560S), using d(T)23VN and 600 ng total RNA. The piece of DNA encoding epitope, 550AA600 of ELMSAN1 (Q6PJG2),

(203) Adropin decreases endothelial monolayer permeability after cell-free hemoglobin exposure and reduces MCP-1-induced macrophage transmigrationBiochemical and biophysical research communicationsDecember 10, 2021Dodd WS, Patel D, Lucke-Wold B, Hosaka K, Chalouhi N, Hoh BL.Germany) and reverse transcription was performed using kit obtained from New England Biolabs (Cat. #: E6560, Ipswich, MA). cDNA was amplified using a master mix kit (Cat. #: 1725271, BioRad, Hercules, CA) and

(204) SARS-CoV-2 Genome Sequences, Including One with an ORF7a Deletion, Obtained from Patients in BangladeshMicrobiology Resource AnnouncementsDecember 9, 2021Mizanur Rahman, Rummana Rahim, Abu Hasan, Naoko Kawai, Lavel Chinyama Moonga, Tatsuki Sugi, Kyoko Hayashida, Junya Yamagishinucleic isolation kit (Thermo Fisher Scientific). The ProtoScript II first-strand cDNA synthesis kit (E6560S; NEB) was used to synthesize cDNAs with oligo(dT) primers. Multiplex PCRs were conducted, using cDNAs

(205) Molecular evidence of SARS-CoV-2 in New York before the first pandemic waveNature CommunicationsDecember 1, 2021Matthew M. Hernandez et al.sequencing was performed on the Illumina MiSeq platform following ProtoScript II (New England Biolabs, E6560) complementary DNA synthesis with random hexamers, SARS-CoV-2 whole-genome amplification with custom-designed

(206) Neurovascular protection by adropin in experimental ischemic stroke through an endothelial nitric oxide synthase-dependent mechanismRedox biologyDecember 1, 2021Yang C, Lavayen BP, Liu L, Sanz BD, DeMars KM, Larochelle J, Pompilus M, Febo M, Sun YY, Kuo YM, et al.RNA (1 μg) was transcribed into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (Cat. No. E6560; New England BioLabs). Quantitative real-time PCR was performed with 20 ng of cDNA in a total reaction

(207) Modulation of bioelectric cues in the evolution of flying fishesCurrent BiologyNovember 22, 2021Jacob M. Daane, Nicola Blum, Jennifer Lanni, Helena Boldt, M. Kathryn Iovine, Charles W. Higdon, Stephen L. Johnson, Nathan R. Lovejoy, Matthew P. Harris,New England Biolabs,Cat# B7002S ProtoScript II First Strand cDNA Synthesis Kit,New England Biolabs,Cat# E6560S TURBO DNA-Free Kit,Thermo Fischer Scientific,Cat# AM1907 Deposited data,Deposited data,Deposited data longfin

(208) Increased fidelity of protein synthesis extends lifespanCell metabolismNovember 2, 2021Martinez-Miguel VE, Lujan C, Espie-Caullet T, Martinez-Martinez D, Moore S, Backes C, Gonzalez S, Galimov ER, Brown AEX, Halic M, et al.RNA was converted to cDNA using random hexamers and ProtoScript II Enzyme mix (New England Biolabs; E6560S). Quantitative PCR was performed using Power SYBR Green PCR Master Mix (ThermoFisher Scientific; 4367659),

(209) Parasitic nematode fatty acid- and retinol-binding proteins compromise host immunity by interfering with host lipid signaling pathwaysPLOS PathogensOctober 29, 2021Sophia C. Parks et al.transcription of RNA using ProtoScript II First Strand cDNA synthesis kit (New England BioLabs, NE, E6560L) following the manufacturer protocol, in a MultiGene OptiMax Thermal Cycler (Labnet international, NJ).

(210) Shift in MSL1 alternative polyadenylation in response to DNA damage protects cancer cells from chemotherapeutic agent-induced apoptosisCell reportsOctober 12, 2021Kunisky AK, Anyaeche VI, Herron RS, Park CY, Hwang HW.gels,Invitrogen,Cat#EA0375BOX ProtoScript II First Strand cDNA Synthesis Kit,New England Biolabs,Cat#E6560L DNase I,Invitrogen,Cat#18068015 Trizol reagent,Invitrogen,Cat#15596018 PerfeCTa SYBR Green SuperMix,QuantaBio,Cat#95054–500 Critical

(211) Anisotropic expansion of hepatocyte lumina enforced by apical bulkheadsThe Journal of cell biologyOctober 4, 2021Belicova L, Repnik U, Delpierre J, Gralinska E, Seifert S, Valenzuela JI, Morales-Navarrete HA, Franke C, Räägel H, Shcherbinina E, et al.with DTT. cDNA was synthesized using the ProtoScriptII First Strand cDNA Synthesis Kit (NEB; cat. no. E6560S), following the manufacturer’s protocol with the Random Primer Mix and the RNA denaturation step. qPCR

(212) Prior Exposure to Coxsackievirus A21 Does Not Mitigate Oncolytic Therapeutic EfficacyCancers (Basel)September 4, 2021William J. Burnett, David M. Burnett, Gennie Parkman, Andrew Ramstead, Nico Contreras, William Gravley, Sheri L. Holmen, Matthew A. Williams, Matthew W. VanBrocklinsamples using the ProtoScript II first strand cDNA synthesis kit (New England BioLabs; Ipswich, MA; USA E6560S) with a CVA21-specific reverse primer (Rev 5′-gagtcgagccatcggcggtac-3′). The reaction was carried out

(213) Drosophila STING protein has a role in lipid metabolismeLifeSeptember 1, 2021Akhmetova K, Balasov M, Chesnokov I.generated from 1 μg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560). RT-qPCR analysis was performed in Luna Universal qPCR Master Mix (New England Biolabs, #M3003) using

(214) Targeted RNA-seq improves efficiency, resolution, and accuracy of allele specific expression for human term placentasG3 Genes|Genomes|GeneticsAugust 7, 2021Weisheng Wu, Jennie L Lovett, Kerby Shedden, Beverly I Strassmann, Claudius Vincenzimmediately after DNaseI digestion of placental RNA with the ProtoScript®II First Strand cDNA Synthesis Kit (E6560, New England Biolabs) and random hexamer primers. Qiagen PyroMark Assay Design 2.0 software was used

(215) Baculovirus actin-rearrangement-inducing factor ARIF-1 induces the formation of dynamic invadosome clustersMolecular Biology of the CellAugust 1, 2021Domokos I. Lauko, Taro Ohkawa, Sergio E. Mares, Matthew D. Welchreverse-transcribed to cDNA using a Protoscript II First Strand DNA Synthesis kit (New England Biolabs; E6560S) using random hexamers as primers. S. frugiperda cortactin-specific primers were used to PCR-amplify

(216) Baculovirus actin-rearrangement-inducing factor ARIF-1 induces the formation of dynamic invadosome clustersMol Biol CellAugust 1, 2021Domokos I. Lauko, Taro Ohkawa, Sergio E. Mares, Matthew D. Welchreverse-transcribed to cDNA using a Protoscript II First Strand DNA Synthesis kit (New England Biolabs; E6560S) using random hexamers as primers. S. frugiperda cortactin-specific primers were used to PCR-amplify

(217) Structure and analysis of nanobody binding to the human ASIC1a ion channeleLifeJuly 28, 2021Wu Y, Chen Z, Sigworth FJ, Canessa CM.200521,Mutagenesis of DNA Commercial assay or kit,ProtoScrript II First strand cDNA,New England Biolabs,Cat#: E6560L,Synthesis of single strand DNA "Chemical compound, drug",Pierce anti-HA magnetic beads,Thermo Fisher Scientific

(218) Reciprocal priming between receptor tyrosine kinases at recycling endosomes orchestrates cellular signalling outputsThe EMBO journalJuly 15, 2021Smith MP, Ferguson HR, Ferguson J, Zindy E, Kowalczyk KM, Kedward T, Bates C, Parsons J, Watson J, Chandler S, et al.tests),Cambridge Bioscience,11‐1100 ProtoScript; II First Strand cDNA Synthesis Kit,New England Biolabs,E6560L ReliaPrep RNA Cell Miniprep System,Promega,Z6011 Tumor dissociation kit,Miltenyi Biotec,130‐095‐929 Isolate II PCR and Gel kit

(219) Orphan CpG islands amplify poised enhancer regulatory activity and determine target gene responsivenessNature geneticsJuly 1, 2021Pachano T, Sánchez-Gaya V, Ealo T, Mariner-Faulí M, Bleckwehl T, Asenjo HG, Respuela P, Cruz-Molina S, Muñoz-San Martín M, Haro E, et al.845-KS-2040250). cDNA was generated using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L). RT-qPCRs were performed on the Light Cycler 480II (Roche) using Eef1a1 and Hptr as housekeeping genes.

(220) Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shorteningCell reportsMay 11, 2021Tichy ED, Ma N, Sidibe D, Loro E, Kocan J, Chen DZ, Khurana TS, Hasty P, Mourkioti F.Assay,Sekisui Diagnostics,Cat# 326-10 Protoscript II cDNA First Strand Synthesis Kit,New England Biolabs,Cat# E6560S Qiafilter Plasmid Midi Kit,QIAGEN,Cat# 12243 Rneasy Plus Micro Kit,QIAGEN,Cat# 74034 Trichrome Staining

Be the First to Write a Review!

ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

New England Biolabs

Citations (276)

Reviews

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map