ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(141) Spheroid Culture System, a Promising Method for Chondrogenic Differentiation of Dental Mesenchymal Stem CellsBiomedicinesApril 28, 2023Caroline Mélou, Pascal Pellen-Mussi, Solen Novello, Damien Brézulier, Agnès Novella, Sylvie Tricot, Pascale Bellaud, Dominique Chauvel-Lebret(cDNA) was performed with the cDNA synthesis kit Protoscript First Strand cDNA Synthesis Kit® (Biolabs E6560, New England Biolabs, Ipswich, MA, USA). Quantitative RT-PCR was achieved with a SYBR® Green PCR kit

(142) Rapid viral metagenomics using SMART-9N amplification and nanopore sequencingWellcome Open ResearchApril 24, 2023Ingra M. Claro, Mariana S. Ramundo, Thais M. Coletti, Camila A. M. da Silva, Ian N. Valenca, Darlan S. Candido, Flavia C. S. Sales, Erika R. Manuli, Jaqueline G. de Jesus, Anderson de Paula, et al.(Cat No. N8080127, Thermo Fisher Scientific, USA) and ProtoScript II reverse transcriptase (Cat No. E6560, New England BioLabs, USA) according to the manufacturer's instructions. The cDNA was then amplified

(143) Identification of actionable targets for breast cancer intervention using a diversity outbred mouse modeliScienceApril 21, 2023Jennifer B. Jacob, Kuang-Chung Wei, Gerold Bepler, Joyce D. Reyes, Andi Cani, Lisa Polin, Kathryn White, Seongho Kim, Nerissa Viola, Julie McGrath, et al.kit,10X Genomics,Cat#1000121 ProtoScript II first strand cDNA synthesis kit,New England Biolabs,Cat#E6560S iTaq universal SYBR Green supermix,Bio-Rad Laboratories,Cat#1725120 GigaMUGA,Neogen,Cat#550 ,, Deposited

(144) The non-canonical Wnt receptor Ror2 is required for cartilage cell polarity and morphogenesis of the craniofacial skeleton in zebrafishDevelopment (Cambridge, England)April 15, 2023Dranow DB, Le Pabic P, Schilling TF.transcription reaction to produce cDNA (Proscript II First Strand cDNA Synthesis Kit, New England Biolabs, E6560L). Zebrafish Ror1 and Ror2 constructs were amplified from 2 dpf cDNA and sfGFP fusion constructs were

(145) A Drosophila screen identifies a role for histone methylation in ER stress preconditioningbioRxivMarch 15, 2023Katie G. Owings, Clement Y. Chowstep. RNA was converted to cDNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix

(146) Parasitic nematode secreted phospholipase A2 suppresses cellular and humoral immunity by targeting hemocytes in Drosophila melanogasterFrontiers in ImmunologyMarch 15, 2023Sophia C. Parks, Ogadinma K. Okakpu, Pakeeza Azizpor, Susan Nguyen, Stephanie Martinez-Beltran, Isaiah Claudio, Kyle Anesko, Anil Bhatia, Harpal S. Dhillon, Adler R. Dillmantranscription of RNA was done using ProtoScript II First Strand cDNA synthesis kit (New England BioLabs, NE, E6560L) following the manufacturer protocol, in a MultiGene OptiMax Thermal Cycler (Labnet international, NJ).

(147) Multiplexed kinase interactome profiling quantifies cellular network activity and plasticityMolecular cellMarch 2, 2023Golkowski M, Lius A, Sapre T, Lau HT, Moreno T, Maly DJ, Ong SE.Substrate,Bio-Rad,Cat#: 1705060 Protoscript II First Strand cDNA Synthesis Kit,New England Biolabs,Cat#: E6560L Deposited Data,Deposited Data,Deposited Data All MS raw files and MaxQuant output files,"MassIVE Repository

(148) CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in miceSTAR ProtocolsMarch 1, 2023Annelise Cassidy, Stephane Pelletier(https://www.agilent.com/cs/library/usermanuals/public/G2964-90000_TapeStation_USR_ENU.pdf).34.Using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L) with the d(T)23 VN primer, produce cDNA from 100 ng of total RNA, as described by the manufacturer

(149) NEK1-Mediated Phosphorylation of YAP1 Is Key to Prostate Cancer ProgressionBiomedicinesFebruary 28, 2023Ghosh I, Khalil MI, Mirza R, King J, Olatunde D, De Benedetti A.1 µg of total RNA per reaction, complementary DNA (cDNA) was synthesized (New England Biolab, cat# E6560S, Ipswich, Massachusetts, USA). iQ SYBR green supermix (Biorad, cat# 1708880, Des Plaines, IL, USA) and

(150) S1P Released by SGPL1-Deficient Astrocytes Enhances Astrocytic ATP Production via S1PR2,4, Thus Keeping Autophagy in Check: Potential Consequences for Brain HealthInternational Journal of Molecular SciencesFebruary 26, 2023Shah Alam, Sumaiya Yasmeen Afsar, Gerhild Van Echten-Deckertreverse transcription with the ProtoScript® II First Strand cDNA Synthesis kit (New England Biolabs, E6560L, Frankfurt am Main, Germany,). The resulting total cDNA was then applied to real-time PCR (CFX96-real time PCR

(151) Endothelial cell expression of mutant Map2k1 causes vascular malformations in miceAngiogenesisFebruary 1, 2023Patrick J. Smits, Christopher L. Sudduth, Dennis J. Konczyk, Yu Sheng Cheng, Matthew P. Vivero, Harry P. W. Kozakewich, Matthew L. Warman, Arin K. Greeneperformed by generating cDNA with the Protoscript II kit using the oligo-dT primer (New England Biolabs: E6560) and then performing ddPCR to detect wild-type and missense mutant transcripts. ddPCR primers and probes

(152) A fluid biomarker reveals loss of TDP-43 splicing repression in pre-symptomatic ALSbioRxiv : the preprint server for biologyJanuary 24, 2023Irwin KE, Jasin P, Braunstein KE, Sinha I, Bowden KD, Moghekar A, Oh ES, Raitcheva D, Bartlett D, Berry JD, et al.74104). cDNA was derived from total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S). Numerous primers were designed against cryptic exon targets and screened to identify primer pairs

(153) Mechanical stimulation promotes MSCs healing the lesion of intervertebral disc annulus fibrosusFrontiers in bioengineering and biotechnologyJanuary 1, 2023Deng R, Kang R, Jin X, Wang Z, Liu X, Wang Q, Xie L.according to the manufacturer’s instructions. The RNA was synthesized by reverse transcription kit (E6560, NEB, United States) to cDNA, followed by quantitative real-time PCR (qPCR, ABI StepOnePlus, United

(154) Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programmingSTAR protocolsDecember 16, 2022Lynn MA, Ryan FJ, Tee YC, Lynn DJ.kit,Life Technologies,Cat#AM1906 ProtoScript® II First Strand cDNA Synthesis Kit,New England Biolabs,Cat#E6560S Oligo(dT)12-18 Primer,Invitrogen via TFS,Cat#18418012 Deoxynucleotide (dNTP) solution mix,New England Biolabs

(155) The Caenorhabditis elegans ARIP-4 DNA helicase couples mitochondrial surveillance to immune, detoxification, and antiviral pathwaysProceedings of the National Academy of SciencesDecember 6, 2022Kai Mao, Peter Breen, Gary RuvkunThe cDNA was generated by the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L). qPCR was performed toward cyp-14A4, ugt-8, clec-144, cnc-9, and hsp-6 with act-1 as control by the

(156) Phylogenetic analysis and comparative genomics of SARS-CoV-2 from survivor and non-survivor COVID-19 patients in Cordoba, ArgentinaBMC GenomicsDecember 1, 2022Nadia B. Olivero, Ana S. Gonzalez-Reiche, Viviana E. Re, Gonzalo M. Castro, MarÃa B. Pisano, Paola Sicilia, MarÃa G. Barbas, Zenab Khan, Adriana van de Guchte, Jayeeta Dutta, et al.complementary DNA (cDNA) synthesis with random hexamers using ProtoScript II (New England Biolabs, E6560), followed by whole-genome amplification with custom-designed tiling primers and library preparation

(157) Fusion protein-driven IGF-IR/PI3K/AKT signals deregulate Hippo pathway promoting oncogenic cooperation of YAP1 and FUS-DDIT3 in myxoid liposarcomaOncogenesisDecember 1, 2022Ruth Berthold et al.(QIAGEN, #74134), followed by cDNA synthesis with the ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560) according to the manufacturer’s instructions. Quantitative reverse transcription PCR (RT-qPCR) was

(158) One-step generation of a conditional allele in mice using a short artificial intronHeliyonDecember 1, 2022Cassidy AM, Thomas DB, Kuliyev E, Chen H, Pelletier S.100 ng of total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L) with the d(T)23 VN primer, according to the manufacturer's recommendations. Scyl1 transcript expression

(159) Polymer nanoparticles pass the plant interfaceNature CommunicationsNovember 30, 2022Sam J. Parkinson, Sireethorn Tungsirisurp, Chitra Joshi, Bethany L. Richmond, Miriam L. Gifford, Amrita Sikder, Iseult Lynch, Rachel K. O’Reilly, Richard M. Napierreverse transcribed using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs; E6560L) following manufacturer’s instructions in a 20 μL reaction volume with the included Random Primer Mix

(160) Lipopolysaccharide induces placental mitochondrial dysfunction in murine and human systems by reducing MNRR1 levels via a TLR4-independent pathwayiScienceNovember 18, 2022Purandare N, Kunji Y, Xi Y, Romero R, Gomez-Lopez N, Fribley A, Grossman LI, Aras S.89874 MitoSOX Red,Thermo Fisher,Cat # M36008 Protoscript II cDNA synthesis kit,New England Biolabs,Cat # E6560 QuikChange Lightning Site-Directed Mutagenesis Kit,Agilent,Cat # 210518 RNAeasy RNA isolation kit,Qiagen,Cat

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ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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