ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(101) Epidermal retinol dehydrogenases cyclically regulate stem cell markers and clock genes and influence hair compositionCommunications BiologyApril 12, 2024Kelli R. Goggans, Olga V. Belyaeva, Alla V. Klyuyeva, Jacob Studdard, Aja Slay, Regina B. Newman, Christine A. VanBuren, Helen B. Everts, Natalia Y. Kedishvili(Thermo Scientific). ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs, catalog no. E6560) was used for cDNA strand synthesis from 1 µg sample RNA. One WT mouse sample was used for several extra

(102) Pathophysiology of chikungunya virus infection associated with fatal outcomesCell host & microbeApril 10, 2024de Souza WM, Fumagalli MJ, de Lima STS, Parise PL, Carvalho DCM, Hernandez C, de Jesus R, Delafiori J, Candido DS, Carregari VC, et al.(LXSAHM) – 12 Plex,R&D Systems,LXSAHM ProtoScript® II First Strand cDNA Synthesis Kit,New England Biolabs,E6560L Q5 High-Fidelity DNA polymerase,New England Biolabs,M0491L Agencourt AMPure XP,Beckman Coulter,A63880 Qubit

(103) Systematic mapping of TF-mediated cell fate changes by a pooled induction coupled with scRNA-seq and multi-omics approachesGenome ResearchApril 5, 2024Muyoung Lee, Qingqing Guo, Mijeong Kim, Joonhyuk Choi, Alia Segura, Alper Genceroglu, Lucy LeBlanc, Nereida Ramirez, Yu Jin Jang, Yeejin Jang, et al.reverse-transcribed with the ProtoScript II first strand cDNA synthesis kit (New England Biolabs [NEB] E6560). Each gene of interest was amplified from the cDNA and cut with NotI and/or NheI depending on the presence

(104) Dissection of a CTCF topological boundary uncovers principles of enhancer-oncogene regulationMolecular CellApril 4, 2024Kyung Lock Kim, Gilbert J. Rahme, Viraat Y. Goel, Chadi A. El Farran, Anders S. Hansen, Bradley E. Bernstein74134) and used to synthesize cDNA with the ProtoScript II First Strand cDNA Synthesis Kit (NEB no. E6560). cDNA was analyzed using the SYBR mastermix (Applied Biosystems) on a 7500 Fast Real Time system (Applied

(105) Analysis of the Mycotoxin Levels and Expression Pattern of SWN Genes at Different Time Points in the Fungus Slafractonia leguminicolaMicroorganismsMarch 27, 2024Sumanjari Das, Dale R. Gardner, Daniel Cook, Rebecca Creamerisolated total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs # E6560L, Ipswich, MA, USA) following the manufacturer’s instruction. 2.5. Quantitative Real-Time PCR (RT-qPCR) Quantified

(106) Gα11 deficiency increases fibroblast growth factor 23 levels in a mouse model of familial hypocalciuric hypercalcemiaJCI insightMarch 26, 2024Ay B, Cyr SM, Klovdahl K, Zhou W, Tognoni CM, Iwasaki Y, Rhee EP, Dedeoglu A, Simic P, Bastepe M.using the RNeasy Mini Kit (74104, Qiagen) and converted into cDNA using a first-strand synthesis kit (E6560, New England BioLabs). cDNAs used to detect Fgf23 gene expression were prepared with oligo(dT) and a

(107) An in vitro study elucidating the synergistic effects of aqueous cinnamon extract and an anti-TNF-α biotherapeutic: implications for a complementary and alternative therapy for non-respondersBMC Complement Med TherMarch 23, 2024Shubrata Khedkar, Minhaj Ahmad Khanlow-volume plates on a Synergy microplate reader (BioTek). cDNA was prepared using Protoscript II (E6560, NEB, Ipswich, MA, USA) per the product datasheet. qRT-PCR was performed using the PowerUp™ SYBR™ Green master mix

(108) Integrative high-throughput enhancer surveying and functional verification divulges a YY2-condensed regulatory axis conferring risk for osteoporosisCell GenomicsMarch 13, 2024Xiao-Feng Chen, Yuan-Yuan Duan, Ying-Ying Jia, Qian-Hua Dong, Wei Shi, Yan Zhang, Shan-Shan Dong, Meng Li, Zhongbo Liu, Fei Chen, et al.Cat#T2030S, USA). Subsequently, first strand cDNA synthesis was performed using RT-UMI (Table S6) (NEB, Cat#E6560S, USA). Next, cDNA was treated with RNase A (Takara, Cat#2158, Japan) and purified with AMPure XP beads.

(109) Design and synthesis of synthetic promoters for consistency of gene expression across growth phases and scale in S. cerevisiaeSynth Syst BiotechnolMarch 13, 2024Kristin V. Presnell, Omar Melhem, Sarah M. Coleman, Nicholas J. Morse, Hal S. Alpereach RNA extraction was used with the NEB ProtoScript® II First Strand cDNA Synthesis Kit (catalog #E6560S) and the Oligo d(T)23 VN primer. No enzyme and no RNA controls were also run. 2.9 RT-qPCR and analysis RT-qPCR

(110) FTO attenuates TNF-α-induced damage of proximal tubular epithelial cells in acute pancreatitis-induced acute kidney injury via targeting AQP3 in an N6-methyladenosine-dependent mannerRen FailMarch 6, 2024Xinghui Li, Qi Liang, Lu Liu, Shujun Chen, Yong Li, Yu Puextracted, and the concentration was tested. After cDNA synthesis, a commercial cDNA synthesis kit (E6560S, New England Biolabs, Inc., USA), CFX384 Touch Real-Time PCR Detection System (Bio-Rad, USA), and SYBR

(111) Drosophila models of phosphatidylinositol glycan biosynthesis class A congenital disorder of glycosylation (PIGA-CDG) mirror patient phenotypesG3: Genes, Genomes, GeneticsMarch 6, 2024Holly J Thorpe, Katie G Owings, Miriam C Aziz, Madelyn Haller, Emily Coelho, Clement Y ChowRNA was used to synthesize cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB Cat #E6560L). Fifty nanogram of cDNA was used to perform quantitative PCR (qPCR) with PowerUp SYBR Green Master Mix

(112) Bi-allelic variants in CELSR3 are implicated in central nervous system and urinary tract anomaliesNPJ Genom MedMarch 1, 2024Jil D. Stegmann, Jeshurun C. Kalanithy, Gabriel C. Dworschak, Nina Ishorst, Enrico Mingardo, Filipa M. Lopes, Yee Mang Ho, Phillip Grote, Tobias T. Lindenberg, Öznur Yilmaz, et al.rt-PCR using ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs GmbH, Catalog No. E6560). cDNA Amplification of 2138 bp upstream of exon 1 to exon 2 transition of transcript celsr3-204 was

(113) A Drosophila screen identifies a role for histone methylation in ER stress preconditioningG3: Genes, Genomes, GeneticsFebruary 7, 2024Katie G Owings, Clement Y Chowstep. RNA was converted to cDNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix

(114) A fluid biomarker reveals loss of TDP-43 splicing repression in presymptomatic ALS–FTDNature MedicineFebruary 1, 2024Katherine E. Irwin, Pei Jasin, Kerstin E. Braunstein, Irika R. Sinha, Mark A. Garret, Kyra D. Bowden, Koping Chang, Juan C. Troncoso, Abhay Moghekar, Esther S. Oh, et al.Complementary DNA was derived from total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB, no. E6560S). Numerous primers were designed against cryptic exon targets and screened to identify primer pairs

(115) Harnessing non-Watson–Crick’s base pairing to enhance CRISPR effectors cleavage activities and enable gene editing in mammalian cellsProceedings of the National Academy of SciencesJanuary 9, 2024Shuliang Gao, Huiwen Guan, Hanan Bloomer, Douglas Wich, Donghui Song, Jennifer Khirallah, Zhongfeng Ye, Yu Zhao, Mengting Chen, Chutian Xu, et al.cDNA Synthesis. cDNA synthesis was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560) with 200 ng ATP-mRNA or 600 ng ZTP-mRNA in a total reaction volume of 20 µL. Reactions were incubated

(116) Diversification of small RNA pathways underlies germline RNA interference incompetence in wild Caenorhabditis elegans strainsGENETICSJanuary 3, 2024Han Ting Chou, Francisco Valencia, Jacqueline C Alexander, Avery Davis Bell, Diptodip Deb, Daniel A Pollard, Annalise B PaabyScientific), and cDNA was synthesized using the ProtoScript II First Stand cDNA Synthesis Kit (NEB #E6560S). The experiment was replicated as follows: from each experimental condition, we collected 2 RNA samples,

(117) Therapeutic potential of archaeal unfoldase PANet and the gateless T20S proteasome in P23H rhodopsin retinitis pigmentosa micePloS oneJanuary 1, 2024Brooks C, Kolson D, Sechrest E, Chuah J, Schupp J, Billington N, Deng WT, Smith D, Sokolov M.the RNeasy Mini Kit (74104, Qiagen) and reverse transcribed into cDNA using the Protoscript II Kit (E6560S, New England Biolabs). Samples were analyzed by qPCR using Brilliant II SYBR Green qPCR Master Mix (600828,

(118) Novel strains of Culex flavivirus and Hubei chryso-like virus 1 from the Anopheles mosquito in western KenyaVirus ResearchJanuary 1, 2024Olivia Wesula Lwande, Jonas Näslund, Andreas Sjödin, Rebecca Lantto, Verah Nafula Luande, Göran Bucht, Clas Ahlm, Bernard Agwanda, Vincent Obanda, Magnus Evanderet al., 2021). The RNA was converted to cDNA using ProtoScript II First Strand cDNA Synthesis Kit (E6560S) and New England Biolab's Random Primer 6 (S1230S). The NEBNext Ultra II Non-Directional RNA Second

(119) A robust knock-in approach using a minimal promoter and a minicircleDevelopmental BiologyJanuary 1, 2024Margaret Keating, Ryan Hagle, Daniel Osorio-Méndez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kangsynthesized from 300 ng to 1 μg of total RNA using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). The sequences of the primers used for RT-PCR are listed in Table S2. Imaging, immunostaining, and

(120) leptin b and its regeneration enhancer illustrate the regenerative features of zebrafish heartsDevelopmental dynamics : an official publication of the American Association of AnatomistsJanuary 1, 2024Shin K, Begeman IJ, Cao J, Kang J.Complementary DNA (cDNA) was synthesized using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). Quantitative PCR was performed using the qPCRBIO SyGreen Blue Mix Separate-ROX (Genesee Scientific,

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ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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