ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(181) The impact of thigmotaxis deprivation on the development of the German cockroach (Blattella germanica)iScienceAugust 19, 2022Yun-Ru Chen, De-Wei Li, Hsin-Ping Wang, Shih-Shun Lin, En-Cheng Yangbeads,New England BioLabs,S1419S ProtoScript® II First Strand cDNA Synthesis Kit,New England BioLabs,E6560S DNA polymerase I (E. coli),New England BioLabs,M0209S Rnase H,New England BioLabs,M0297S NEBNext® Ultra™ II End Repair/dA-Tailing Module

(182) Voltage-gated sodium channel scn8a is required for innervation and regeneration of amputated adult zebrafish finsProceedings of the National Academy of Sciences of the United States of AmericaJuly 12, 2022Osorio-Méndez D, Miller A, Begeman IJ, Kurth A, Hagle R, Rolph D, Dickson AL, Chen CH, Halloran M, Poss KD, et al.300 ng to 1 μg of total RNA using an NEB ProtoScript II first-strand cDNA synthesis kit (catalog no. E6560). For RT-PCR, HuC/elavl3 was used for the brain-specific marker; and4 was used for regenerating fin

(183) Expression pattern of Stomatin-domain proteins in the peripheral olfactory systemScientific reportsJuly 6, 2022Gonzalez-Velandia KY, Hernandez-Clavijo A, Menini A, Dibattista M, Pifferi S.DNA. cDNA was synthetized using the Protoscript II First-Strand DNA Synthesis Kit from 100 ng of mRNA (E6560S, New England Biolabs). PCR was performed in thermocycler (ThermaCycler2720, LifeTechnologies) using

(184) Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerabilityNature chemical biologyJuly 1, 2022Li Z, Ferguson L, Deol KK, Roberts MA, Magtanong L, Hendricks JM, Mousa GA, Kilinc S, Schaefer K, Wells JA, et al.of RNA samples were reverse transcribed using Protoscript II First Strand cDNA Synthesis Kit (NEB #E6560S). cDNA was measured using a QuantStudio 7 Flex Real-time PCR machine (Applied Biosystems) with Power

(185) Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinomaInternational journal of oncologyJuly 1, 2022Huang X, Huang Y, Lv Z, Wang T, Feng H, Wang H, Du S, Wu S, Shen D, Wang C, et al.as isopropanol precipitation. Reverse transcription was then conducted using a cDNA synthesis kit (E6560S, New England Biolabs) at 42°C for 15 min. Subsequent quantitative polymerase chain reaction (qPCR) analyses

(186) Vision-related convergent gene losses reveal SERPINE3â€™s unknown role in the eyeeLifeJune 21, 2022Henrike Indrischek, Juliane Hammer, Anja Machate, Nikolai Hecker, Bogdan Kirilenko, Juliana Roscito, Stefan Hans, Caren Norden, Michael Brand, Michael Hillerkit,Qiagen,"Cat. #: 75,144", commercial assay or kit,ProtoScript II First Strand Synthesis kit,NEB,Cat. #: E6560S, commercial assay or kit,TSA Plus Cy3/Cy5 kit,Perkin-Elmer,Cat. #: NEL744001KT, commercial assay or

(187) Vision-related convergent gene losses reveal SERPINE3’s unknown role in the eyeElifeJune 21, 2022Henrike Indrischek, Juliane Hammer, Anja Machate, Nikolai Hecker, Bogdan Kirilenko, Juliana Roscito, Stefan Hans, Caren Norden, Michael Brand, Michael Hillerkit,Qiagen,"Cat. #: 75,144", commercial assay or kit,ProtoScript II First Strand Synthesis kit,NEB,Cat. #: E6560S, commercial assay or kit,TSA Plus Cy3/Cy5 kit,Perkin-Elmer,Cat. #: NEL744001KT, commercial assay or

(188) Oxidative stress from DGAT1 oncoprotein inhibition in melanoma suppresses tumor growth when ROS defenses are also breachedCell reportsJune 21, 2022Wilcock DJ, Badrock AP, Wong CW, Owen R, Guerin M, Southam AD, Johnston H, Telfer BA, Fullwood P, Watson J, et al.CellEvent Caspase-3/7 Green Detection Reagent,Life Technologies,C10423 ProtoScript; II First Strand cDNA Synthesis Kit,New England Biolabs,E6560L RNeasy RNA Extraction Kit,Qiagen,74136 ,, Deposited data,Deposited data,Deposited data ,, Proteome

(189) Nuclear soluble cGAS senses double-stranded DNA virus infectionCommunications biologyMay 10, 2022Wu Y, Song K, Hao W, Li J, Wang L, Li S.transcribed into cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs®, E6560L). For one real-time reaction, 5 µL of Maxima SYBR Green/ROX qPCR Master Mix (2×) (Thermo Scientific),

(190) Phylogenetic-based inference reveals distinct transmission dynamics of SARS-CoV-2 lineages Gamma and P.2 in BraziliScienceApril 15, 2022Tiago Gräf, Gonzalo Bello, Felipe Gomes Naveca, Marcelo Gomes, Vanessa Leiko Oikawa Cardoso, Alexandre Freitas da Silva, Filipe Zimmer Dezordi, Mirleide Cordeiro dos Santos, Katia Correa de Oliveira Santos, Érika Lopes Rocha Batista, et al.house,https://www.fda.gov/media/134922/download PROTOSCRIPT® II FIRST STRAND CDNA SYNTHESIS KIT - 150 RX,New England Biolabs,E6560L Q5 Hot Start High-Fidelity DNA Polymerase,ThermoFisher,M0493L Qubit High Sensitivity dsDNA kit,ThermoFisher,Q32851 SuperScript™ IV First-Strand Synthesis System

(191) Efficient Generation and Transcriptomic Profiling of Human iPSC-Derived Pulmonary Neuroendocrine CellsSSRN Electronic JournalApril 3, 2022Pooja Hor et al.and reverse-transcribed to cDNA using a Protoscript first strand synthesis kit (New England Biolabs, E6560S). Quantitative PCR was performed using SYBR Green (Biorad, 1725125) in a 5 μl reaction volume at 50

(192) Profiling SARS-CoV-2 mutation fingerprints that range from the viral pangenome to individual infection quasispeciesmedRxivMarch 31, 2022Billy T. Lau et al.hexamer priming and ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, catalog no. E6560L) according to manufacturer instructions. We generated amplicons from cDNA samples using the Titanium

(193) Comprehensive Genetic Characterisation of Mitochondrial Ca2+ Uniporter Components Reveals Their Different Physiological Requirements in VivoSSRN Electronic JournalMarch 30, 2022Roberta Tufi, Thomas P. Gleeson, Sophia von Stockum, Victoria L. Hewitt, Juliette J. Lee, Ana Terriente-Felix, Alvaro Sanchez-Martinez, Elena Ziviani, Alex WhitworthRNA purification kit QIAGEN QIAGEN: 74106 ProtoScript II first strand cDNA Synthesis Kit New England BioLabs NEB: E6560S DC Protein Assay Kit Bio-Rad N/A (Continued on next page) Cell Reports 27, 1541–1550.e1–e5, April

(194) Influence of Periodontal Ligament Stem Cell-Derived Conditioned Medium on OsteoblastsPharmaceuticsMarch 28, 2022Novello S, Tricot-Doleux S, Novella A, Pellen-Mussi P, Jeanne S.deoxyribonucleic acid (cDNA) using the cDNA synthesis kit Protoscript First Strand cDNA Synthesis Kit® (Biolabs E6560, New England Biolabs, Ipswich, MA, USA). Quantitative RT-PCR was carried out with a SYBR® Green PCR kit

(195) Mutations in SARS-CoV-2 variants of concern link to increased spike cleavage and virus transmissionCell host & microbeMarch 9, 2022Escalera A, Gonzalez-Reiche AS, Aslam S, Mena I, Laporte M, Pearl RL, Fossati A, Rathnasinghe R, Alshammary H, van de Guchte A, et al.Briefly, cDNA synthesis was performed with random hexamers and ProtoScript II (New England Biolabs, E6560) using 7 μL of RNA according to manufacturer’s recommendations. The RT reaction was incubated for 30

(196) Alphacoronavirus in a Daubenton’s Myotis Bat (Myotis daubentonii) in SwedenVirusesMarch 8, 2022Olivia Wesula Lwande, Therese Thalin, Johnny de Jong, Andreas Sjödin, Jonas Näslund, Magnus Evander, Frauke Eckeworkflows. Briefly, the RNA was converted to cDNA using ProtoScript II First Strand cDNA Synthesis Kit (E6560S) and New England Biolab’s Random Primer 6 (S1230S). The NEBNext Ultra II Non-Directional RNA Second

(197) Exploration of the ocular surface infection by SARS-CoV-2 and implications for corneal donation: An ex vivo studyPLoS medicineMarch 1, 2022Maurin C, He Z, Mentek M, Verhoeven P, Pillet S, Bourlet T, Rogues F, Pugniet JL, Peyragrosse T, Barallon M, et al.synthetized from total RNA by reverse transcription with ProtoScript II First Strand cDNA Synthesis Kit (E6560, New England BioLabs). Reverse transcription was conducted during 1 hour at 42°C followed by enzyme

(198) Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display LibraryAntibodies (Basel, Switzerland)February 10, 2022Mendoza-Salazar I, Gómez-Castellano KM, González-González E, Gamboa-Suasnavart R, Rodríguez-Luna SD, Santiago-Casas G, Cortés-Paniagua MI, Pérez-Tapia SM, Almagro JC.the Protoscript® II First Strand cDNA synthesis Kit (New England Biolabs, Ipswich, MA, USA, Cat. No.: E6560S) and a poly-T oligo. Double-stranded DNA encoding the VH repertoire was PCR-amplified with the primers

(199) The B.1.427/1.429 (epsilon) SARS-CoV-2 variants are more virulent than ancestral B.1 (614G) in Syrian hamstersPLOS PathogensFebruary 10, 2022Timothy Carroll et al.first strand cDNA synthesis using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs #E6560) with random hexamers and following manufacturer’s instructions. PCR amplification was performed using

(200) Identification of high-confidence human poly(A) RNA isoform scaffolds using nanopore sequencingRNAFebruary 1, 2022Logan Mulroney et al.first strand cDNA synthesis with random priming using the Protoscript II First Strand Synthesis Kit (NEB, #E6560) in a total volume of 40 µL for 1 h at 42°C. The cDNA was diluted to 150 µL and aliquoted into 96 wells

Be the First to Write a Review!

ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

New England Biolabs

Citations (285)

Reviews

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map