ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(81) Deep mutation, insertion and deletion scanning across the Enterovirus A proteome reveals constraints shaping viral evolutionNature MicrobiologyNovember 28, 2024William Bakhache, Walker Symonds-Orr, Lauren McCormick, Patrick T. DolanKit (74106; QIAGEN). To generate cDNA for sequencing, ProtoScript II First Strand cDNA Synthesis Kit (E6560L; New England Biolabs) was used to generate first-strand cDNA using reverse primers for P1 or P2–P3.

(82) Hepatocyte differentiation requires anisotropic expansion of bile canaliculiDevelopment (Cambridge, England)November 15, 2024Bebelman MP, Belicova L, Gralinska E, Jumel T, Lahree A, Sommer S, Shevchenko A, Zatsepin T, Kalaidzidis Y, Vingron M, et al.with 40 mM DTT. cDNA was synthesized using the ProtoScriptII First Strand cDNA Synthesis Kit (NEB; E6560S), following the manufacturer's protocol with the Random Primer Mix and the RNA denaturation step. qPCR

(83) Harnessing the regenerative potential of interleukin11 to enhance heart repairNature communicationsNovember 8, 2024Shin K, Rodriguez-Parks A, Kim C, Silaban IM, Xia Y, Sun J, Dong C, Keles S, Wang J, Cao J, et al.synthesized from 300 ng to 1 μg of total RNA using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). For RNA-sequencing, total RNA was prepared from three biological replicate pools of 7 dpt or 7 mpt

(84) A drug repurposing screen reveals dopamine signaling as a critical pathway underlying potential therapeutics for the rare disease DPAGT1-CDGPLOS GeneticsOctober 28, 2024Hans M. Dalton, Naomi J. Young, Alexys R. Berman, Heather D. Evans, Sydney J. Peterson, Kaylee A. Patterson, Clement Y. Chowstep. We converted RNA into cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB cat. E6560L). We then used this cDNA, PowerUp SYBR Green Master Mix (ThermoFisher cat. A25741), and forward/reverse

(85) Protofilament-specific nanopatterns of tubulin post-translational modifications regulate the mechanics of ciliary beatingCurrent BiologyOctober 7, 2024Gonzalo Alvarez Viar, Nikolai Klena, Fabrizio Martino, Adrian Pascal Nievergelt, Davide Bolognini, Paola Capasso, Gaia Piginoacrylate,Sigma,408220 acrylamide,Sigma,A4058 Alt-R™ S.p. Cas9 Nuclease V3,IDT,1081058 Protoscript II First Strand cDNA Synthesis Kit,NEB,E6560 DirectZol,Zymo Research,R2050 PlatinumTM SuperFi II PCR Master Mix (2x),Thermo Fisher,12368010 EvaGreen

(86) Polyclonality overcomes fitness barriers in Apc-driven tumorigenesisNatureOctober 1, 2024Sadien ID, Adler S, Mehmed S, Bailey S, Sawle A, Couturier DL, Eldridge M, Adams DJ, Kemp R, Lourenço FC, et al.First strand cDNA synthesis was carried out using a NEB ProtoScript II First Strand cDNA synthesis kit (E6560) according to the manufacturer’s instructions. Both oligo-dT or a gene specific reverse primer (rev_3699

(87) Subarachnoid hemorrhage-associated brain injury and neurobehavioral deficits are reversed with synthetic adropin treatment through sustained Ser1179 phosphorylation of endothelial nitric oxide synthaseFrontiers in StrokeSeptember 30, 2024William S. Dodd, Devan Patel, Dimitri Laurent, Brandon Lucke-Wold, Koji Hosaka, Richard D. Johnson, Nohra Chalouhi, Andrew A. Butler, Eduardo Candelario-Jalil, Brian L. HohGermany), and reverse transcription was performed using a kit obtained from New England Biolabs (Cat. #: E6560, Ipswich, MA). cDNA was amplified using a master mix kit (Cat. #: 1725271, BioRad, Hercules, CA) and

(88) Evolutionary rate covariation is pervasive between glycosylation pathways and points to potential disease modifiersPLOS GeneticsSeptember 11, 2024Holly J. Thorpe, Raghavendran Partha, Jordan Little, Nathan L. Clark, Clement Y. ChowDNAse step. RNA was converted to cDNA using a ProtoScript II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix

(89) Evaluating metagenomics and targeted approaches for diagnosis and surveillance of virusesGenome MedicineSeptember 9, 2024Sarah Buddle, Leysa Forrest, Naomi Akinsuyi, Luz Marina Martin Bernal, Tony Brooks, Cristina Venturini, Charles Miller, Julianne R. Brown, Nathaniel Storey, Laura Atkinson, et al.cDNA synthesis was performed using ProtoScript II First strand synthesis kit (New England Biolabs, E6560) followed by the NEBNext Ultra Non-Directional Second Strand Synthesis module (New England Biolabs,

(90) RNA interacts with topoisomerase I to adjust DNA topologyMolecular CellSeptember 5, 2024Mannan Bhola, Kouki Abe, Paola Orozco, Homa Rahnamoun, Pedro Avila-Lopez, Elijah Taylor, Nefertiti Muhammad, Bei Liu, Prachi Patel, John F. Marko, et al.3.00μm,Polysciences Inc,Cat# 17145-5 ProtoScript® II First Strand cDNA Synthesis Kit,New England Biolabs,Cat# E6560S QIAquick PCR Purification Kit,Qiagen,Cat# 28106 Qubit 1X dsDNA HS Assay Kit,Thermo Fisher Scientific,Cat#

(91) Large-scale RNA-seq mining reveals ciclopirox triggers TDP-43 cryptic exonsbioRxivAugust 28, 2024Irika R. Sinha, Parker S. Sandal, Grace D. Burns, Aswathy Peethambaran Mallika, Katherine E. Irwin, Anna Lourdes F. Cruz, Vania Wang, Josué Llamas Rodríguez, Philip C. Wong, Jonathan P. LingcDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560L). cDNA was amplified using primer pairs designed to either target two wildtype sequences on either side

(92) Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivoFrontiers in Cell and Developmental BiologyAugust 20, 2024Ivan Anticevic, Cecile Otten, Marta Popovicstored at −80°C. For reverse transcription, the ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560L) was used, following the manufacturer’s instructions. Total RNA from zebrafish tissues and embryos was

(93) Interface-guided phenotyping of coding variants in the transcription factor RUNX1Cell ReportsJuly 23, 2024Kivilcim Ozturk, Rebecca Panwala, Jeanna Sheen, Kyle Ford, Nathan Jayne, Andrew Portell, Dong-Er Zhang, Stephan Hutter, Torsten Haferlach, Trey Ideker, et al.Tissue Kit,Qiagen,Cat#69504 Protoscript II First Strand cDNA Synthesis Kit,New England BioLabs,Cat#E6560S QIAquick PCR Purification Kit,Qiagen,Cat#28104 Nucleofector Solution Set SF,Lonza,Cat#PBC2-00675 Chromium

(94) Comparative immune profiling of pancreatic ductal adenocarcinoma progression among South African patientsBMC CancerJuly 7, 2024Nnenna Elebo, Ebtesam A. Abdel-Shafy, Jones A. O. Omoshoro-Jones, Zanele Nsingwane, Ahmed A. A. Hussein, Martin Smith, Geoffrey Candy, Stefano Cacciatore, Pascaline Fru, Ekene Emmanuel Nwekeperformed from 250 ng/µl of total RNA using the Photoscript® II First Strand cDNA Synthesis Kit (cat no. E6560S, New England BioLabs® Inc. Massachusetts, United States), according to the manufacturer’s instructions. A

(95) Depletion of TDP-43 exacerbates tauopathy-dependent brain atrophy by sensitizing vulnerable neurons to caspase 3-mediated endoproteolysis of tau in a mouse model of Multiple Etiology DementiabioRxivJuly 3, 2024Meghraj S Baghel, Grace D Burns, Margarita Tsapatsis, Aswathy Peethambaran Peethambaran Mallika, Anna Lourdes F Cruz, Tianyu Cao, Xiaoke K Chen, Isabel De La Rosa, Shaelyn R Marx, Yingzhi Ye, et al.cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L). Target sequences were amplified using a modified touchdown PCR protocol (Korbie and Mattick, 2008),

(96) CMTR-1 RNA methyltransferase mutations activate widespread expression of a dopaminergic neuron-specific mitochondrial complex I geneCurrent BiologyJune 17, 2024Joshua D. Meisel, Presli P. Wiesenthal, Vamsi K. Mootha, Gary Ruvkun(Invitrogen AM1906) and cDNA was synthesized by the ProtoScript II First Strand cDNA Synthesis Kit (NEB E6560). Quantitative real-time PCR was performed using iQ SYBR Green Supermix (Biorad) on a BIORAD CFX Real-Time

(97) Amyloid-β neuropathology induces bone loss in male mice by suppressing bone formation and enhancing bone resorptionBone ReportsJune 1, 2024Younghun Jung, Birol Ay, Sajin M. Cyr, Christina M. Tognoni, Kaitlin Klovdahl, Julia Matthias, Qiuxia Cui, Daniel J. Brooks, Mary L. Bouxsein, Isabel Carreras, et al.(catalog no. 74104, Qiagen) and converted into cDNA using a First-Strand Synthesis Kit (catalog no. E6560, New England BioLabs) with or without reverse transcriptase. Bone tissue consisted of cortical bone

(98) All-trans retinoic acid pretreatment of mesenchymal stem cells enhances the therapeutic effect on acute kidney injuryCell Communication and SignalingMay 27, 2024Yifan Zhang, Xiaochen Wang, Yuwei Ji, Haijuan Hong, Xiaodong Geng, Keying Zhang, Zhangning Fu, Guangyan Cai, Xiangmei Chen, Ping Li, et al.Carlsbad, CA, USA), followed by cDNA synthesis with the ProtoScript II First Strand cDNA Synthesis Kit (E6560S, New England Biolabs, Ipswich, MA, USA). The qRT-PCR was executed on an Applied Biosystems 7500 system

(99) Targeting Integrin α3 Blocks β1 Maturation, Triggers Endoplasmic Reticulum Stress, and Sensitizes Glioblastoma Cells to TRAIL-Mediated ApoptosisCellsApril 26, 2024Yuki Kuranaga, Bing Yu, Satoru Osuka, Hanwen Zhang, Narra S. Devi, Sejong Bae, Erwin G. Van Meir(RT-PCR) First-strand cDNA synthesis was performed with ProtoScript II First Strand cDNA Synthesis Kit (E6560, New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol from 1 μg total RNA

(100) Anti‐inflammatory and immunoregulatory effects of colistin sulphate on human PBMCsJournal of Cellular and Molecular MedicineApril 25, 2024Huiling Chen, Tianli Yang, Yiran Xu, Beibei Liang, Xianyong Liu, Yun CaiComplementary DNA (cDNA) was synthesized from total RNA with the First Stand cDNA Synthesis Kit (NEB, E6560S). Quantitative real‐time reverse transcription‐polymerase chain reaction (qRT‐PCR) was carried out using

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ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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