Spectral Flow Cytometers

Spectral Flow Cytometers Spectral flow cytometers capture the full fluorescence spectrum of labeled cells and particles, unlike conventional cytometers, which rely on optical filters to isolate specific wavelengths. Multiple specialized detectors enable the identification of multiple fluorophores simultaneously based on unique spectral fingerprints. Spectral unmixing, analogous to compensation in conventional cytometry, is used to distinguish fluorophores with even closely overlapping emission spectra. As a result, spectral cytometers can accommodate larger, more complex panels, enhancing multiparametric analysis. Selecting a spectral cytometer requires balancing cost with experimental needs, considering factors such as laser count, detector sensitivity, sample format, acquisition speed, and throughput. These specifications directly impact the resolution and efficiency of high-parameter experiments. Consult the instrument manufacturer for more specifications and features.

Spectral Flow Cytometers

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Cytek Aurora<sup>&#8482;</sup> Evo Flow Cytometer
  • Excitation Lasers:
    Inquire
  • Detection:
    Small Particle Resolution: Violet SSC detector capable of resolving small particles down to 70 nm ...
NovoCyte Opteon Spectral Flow Cytometer Systems
  • Excitation Lasers:
    349 nm / 405 nm / 488 nm / 561 nm / 637 nm
  • Detection:
    FSC, SSC
  • Sample Type:
    Manual loading: 12 x 75 mm, 1.5 and 2.0 mL tube
    Sample loader: 40 tube rack for 12 x 75 mm tube, 40 ...
CytoFLEX mosaic Spectral Detection Module

CytoFLEX mosaic Spectral Detection Module

Beckman Coulter Life Sciences

  • Excitation Lasers:
    Excitation Wavelength: 355 nm (20 mW), 375 nm (60 mW), 405 nm (80 mW), 488 nm (50 mW), 561 nm (30 mW...
  • Detection:
    CytoFLEX mosaic Spectral Detection Module
Cytek Aurora<sup>&#8482;</sup> Flow Cytometer

Cytek Aurora™ Flow Cytometer

Cytek Biosciences

  • Detection:
    3 scatter detectors (FSC, SSC-B, SSC-V). And up to 64 high-resolution fluorescent detectors using ...
Invitrogen&#8482; Bigfoot&#8482; Cell Sorter

Invitrogen™ Bigfoot™ Cell Sorter

Thermo Fisher Scientific

  • Excitation Lasers:
    349 nm, 405 nm, 445 nm, 488 nm, 532 nm, 561 nm, 594 nm, 640 nm, 785 nm
  • Detection:
    Up to 60 parameters, including FSC and SSC
  • Sample Type:
    Sample Input: 1.5 mL, 5 mL, and 15 mL
Invitrogen&#8482; Attune&#8482; Xenith&#8482; Flow Cytometer
  • Excitation Lasers:
    Excitation lasers: 349nm, 405 nm, 488 nm, 561 nm, 637 nm, 781 nm
  • Detection:
    Inquire
FP7000 Spectral Cell Sorter

FP7000 Spectral Cell Sorter

Sony Corporation

  • Excitation Lasers:
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  • Detection:
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ID7000&#8482; Spectral Cell Analyzer
  • Excitation Lasers:
    320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
  • Detection:
    32-channels (PMT arrays); can be configured with 186 detectors to detect 44 colors or more
  • Sample Type:
    5-mL (12 x 75-mm) tubes, 96-well and 384-well plates (Autosampler)
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SA3800 Spectral Analyzer

SA3800 Spectral Analyzer

Sony Corporation

  • Excitation Lasers:
    405 nm, 488 nm, 561nm, 638 nm
  • Detection:
    Inquire
  • Sample Type:
    96-well plates: standard, half deep, and deep; 384 standard flat, and 12 x 75-mm 5-mL tubes
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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer

Sony Corporation

  • Excitation Lasers:
    405 nm, 488 nm, 638 nm (2- or 3-laser configurations)
  • Detection:
    FSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
  • Sample Type:
    5-mL tubes
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Cytek Biosciences
Northern Lights
5
4 Figures from 519 Publications
Sony Corporation
ID7000
5
9 Figures from 385 Publications
Sony Corporation
SA3800
4
9 Figures from 349 Publications
Sony Corporation
SP6800
2
2 Figures from 204 Publications
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