Cytek™ Northern Lights Flow Cytometery System from Cytek Biosciences

Product Specs
ItemCytek™ Northern Lights Flow Cytometery System
CompanyCytek Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumberNorthern Lights
Dimensions(WxDxH) 54 x 52 x 52 cm - without loader
(WxDxH) 58.4 x 62 x 52 cm - with loader
QuantityEA
ApplicationsCancer Research, Immuno-oncology, Immunotherapy, Immunophenotyping, Immune Monitoring, Hematology, Cell Therapies, Stem Cell Research, Gene Editing Assays, Quality Control, Cell Cycle Analysis, Apoptosis, Bead Based Assays
DetectionFSC, SSC, up to 38 fluorescent detectors
Abs / Exc1-3 lasers - 405 nm: 100 mW, 488 nm: 50 mW, 640 nm: 80 mW

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Cytek Biosciences

46107 Landing Pkwy

Fremont, CA 94538

United States

Phone: (877) 922-9835

Email: products@cytekbio.com

Company Profile

Website: https://cytekbio.com/

(1) Stimulation of Hemolysis and Eryptosis by β-Caryophyllene OxideLife (Basel)December 4, 2023Sumiah A. Alghareeb, Mohammad A. Alfhili, Jawaher Alsughayyirannexin-V-FITC for 10 min at RT in the dark, and the fluorescence was recorded at Ex/Em of 488/512 nm using a Northern Lights flow cytometer (Cytek Biosciences, Fremont, CA, USA) [21]. 2.10. Cellular Morphology Cell size and

(2) Eriocitrin Disrupts Erythrocyte Membrane Asymmetry through Oxidative Stress and Calcium Signaling and the Activation of Casein Kinase 1α and Rac1 GTPasePharmaceuticals (Basel)December 2, 2023Sumiah A. Alghareeb, Jawaher Alsughayyir, Mohammad A. Alfhilistained with 1% annexin-V-FITC for 10 min at RT, and a total of 10,000 cells were analyzed using a Northern Lights flow cytometer (Cytek Biosciences, Fremont, CA, USA). FITC was stimulated using a blue laser at 488

(3) RIPK3 promotes brain region-specific interferon signaling and restriction of tick-borne flavivirus infectionPLOS PathogensNovember 27, 2023Marissa Lindman, Juan P. Angel, Irving Estevez, Nydia P. Chang, Tsui-Wen Chou, Micheal McCourt, Colm Atkins, Brian P. Daniels1% PFA in PBS (ThermoFisher, #J19943-K2). Data collection and analysis were performed using a Cytek Northern Lights Cytometer (Cytek, Fremont, California) and FlowJo software (Treestar). Data were normalized using a

(4) Dormant tumors circumvent tumor-specific adaptive immunity by establishing a Treg-dominated niche via DKK3JCI InsightNovember 22, 2023Timothy N. Trotter, Carina E. Dagotto, Delila Serra, Tao Wang, Xiao Yang, Chaitanya R. Acharya, Junping Wei, Gangjun Lei, H. Kim Lyerly, Zachary C. Hartmansee Supplemental Table 4). Cells were fixed in 1% paraformaldehyde and analyzed on a BD LSR or Cytek Northern Lights flow cytometer. Compensation was performed using single-color-stained splenocytes or BD CompBeads in

(5) An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptorBlood advancesOctober 24, 2023Khan DMIO, Karmaus PWF, Bach A, Crawford RB, Kaminski NE.using BD FACSCanto-II cell analyzer (BD Biosciences) with FACSDiva software (BD Biosciences) or Cytek Northern Lights full spectral flow cytometer (Freemont, CA). Flow cytometry data were analyzed using FlowJo (version

(6) Tumor-Infiltrating iNKT Cells Activated through c-Kit/Sca-1 Are Induced by Pentoxifylline, Norcantharidin, and Their Mixtures for Killing Murine Melanoma CellsPharmaceuticals (Basel)October 16, 2023Maximiliano V. M. Correa-Lara, Israel Lara-Vega, Minerva Nájera-Martínez, María Lilia Domínguez-López, Elba Reyes-Maldonado, Armando Vega-López420201, USA) and resuspended in 4% paraformaldehyde for immediate flow cytometric evaluation. A Cytek Northern Lights™ cytometer (Biosciences, State College, PA, USA) from the Flow Cytometry-Instrument Center of the National

(7) Streptococcus agalactiae and Escherichia coli Induce Distinct Effector γδ T Cell Responses During Neonatal Sepsis and NeuroinflammationbioRxivOctober 10, 2023Lila T. Witt, Kara G. Greenfield, Kathryn A. KnoopFollowing this staining, samples were washed twice with FACS buffer and samples were acquired on the CyTek Northern Lights Spectral Flow Cytometer (Cytek Biosciences). Intracellular cytokine staining Cells were placed in

(8) Longitudinal evaluation of innate immune responses to three doses of CoronaVac vaccineFrontiers in ImmunologyOctober 2, 2023Cheng Cao, Junfeng Jiang, Min Liu, Yaping Dai, Tianzhi Chang, Tuo Ji, Fang Gong4°C for 30 minutes, cells were resuspended in FACS buffer and kept on ice until acquisition by Cytek™ Northern Lights. Dead cells were routinely excluded from the analysis by staining with 7-AAD (eBioscience). Monocytes

(9) Selection of cross-reactive T cells by commensal and food-derived yeasts drives cytotoxic TH1 cell responses in Crohn’s diseaseNature MedicineOctober 1, 2023Gabriela Rios Martini, Ekaterina Tikhonova, Elisa Rosati, Meghan Bialt DeCelie, Laura Katharina Sievers, Florian Tran, Matthias Lessing, Arne Bergfeld, Sophia Hinz, Susanna Nikolaus, et al.with the inside stain kit (Miltenyi Biotec). Data were acquired on an LSR Fortessa (BD Bioscience) or Northern Lights 3000 (Cytek Biosciences). Screening of expanded T cell lines on 384-well plates was performed on a MACSQuantX

(10) YAP1 expression is associated with survival and immunosuppression in small cell lung cancerCell Death & DiseaseSeptember 26, 2023Peixin Chen, Chenglong Sun, Hao Wang, Wencheng Zhao, Yan Wu, Haoyue Guo, Caicun Zhou, Yayi HeBD Pharmingen) antibodies for 30 minutes at 4 °C. All flow cytometric analyses were performed on a Northern Lights-CLC spectral flow cytometer (Cytek Biosciences, Inc). All flow cytometry data were analyzed by FlowJo

(11) Intrinsic tumor resistance to CAR T cells is a dynamic transcriptional state that is exploitable with low-dose radiationBlood AdvancesSeptember 26, 2023Alexander B. Kim, Ssu-Yu Chou, Solomon Kang, Eric Kwon, Matthew Inkman, Jeff Szymanski, Neal Andruska, Cian Colgan, Jin Zhang, Joanna C. Yang, et al.according to the manufacturer’s protocols. Data were collected using BD LSR-II, BD LSR-Fortessa, and Cytek Northern Lights cytometers. Data were analyzed with FlowJo Software (Treestar/BD Biosciences, RRID: SCR_008520). Cell sorting

(12) An active HIV reservoir during ART is associated with maintenance of HIV-specific CD8+ T cell magnitude and short-lived differentiation statusCell Host & MicrobeSeptember 13, 2023Hiroshi Takata, Julie L. Mitchell, Julian Pacheco, Amélie Pagliuzza, Suteeraporn Pinyakorn, Supranee Buranapraditkun, Carlo Sacdalan, Louise Leyre, Sam Nathanson, Juyeon C. Kakazu, et al.by 2 washes with washing buffer and then fixation in fix buffer. Fixed cells were analyzed using a Northern Lights 3000 cytometer (Cytek Biosciences). The frequency of CD69+4–1BB+IFN-γ+ HIV-specific CD8+ T cells in

(13) Stimulation of Hemolysis and Eryptosis by α-Mangostin through Rac1 GTPase and Oxidative Injury in Human Red Blood CellsMoleculesSeptember 7, 2023Sumiah A. Alghareeb, Jawaher Alsughayyir, Mohammad A. Alfhiliaway from light, and fluorescence (10,000 events) was then measured at Ex/Em of 488/512 nm with the Northern Lights flow cytometer (Cytek Biosciences, Fremont, CA, USA) [14]. 3.9. Cellular Morphology Forward scatter

(14) CD51 labels periosteal injury-responsive osteoprogenitorsFrontiers in PhysiologySeptember 4, 2023Ye Cao, Ivo Kalajzic, Brya G. Matthewsinjury, we used a panel containing 15 cell surface markers in addition to GFP and tdTomato on the Cytek Northern Lights spectral cytometer, and a simpler panel on a BD LSRII. These and other reagents used are shown in Supplementary

(15) Tumor-Specific CD8+ T Cells from the Bone Marrow Resist Exhaustion and Exhibit Increased Persistence in Tumor-Bearing Hosts as Compared to Tumor Infiltrating LymphocytesbioRxivSeptember 2, 2023Elizabeth M. Zawidzka, Luca Biavati, Amy Thomas, Claudio Zanettini, Luigi Marchionni, Robert Leone, Ivan Borrellodata collection, samples were washed with and resuspended in FACS buffer. Data were collected on a Cytek Northern Lights spectral flow cytometer equipped with 3-lasers (405nm, 488nm, 640nm). A full list of the antibodies

(16) Targeting ACC1 in T cells ameliorates psoriatic skin inflammationJournal of Molecular MedicineSeptember 1, 2023Yu-San Kao, Panagiota Mamareli, Ayesha Dhillon-LaBrooy, Philipp Stüve, Gloria Janet Godoy, Lis Noelia Velasquez, Verena Katharina Raker, Beate Weidenthaler-Barth, Fatima Boukhallouk, Francesca Rampoldi, et al.eBiosciences, San Diego, CA) before staining. Cells were acquired on Cytoflex S (Beckman Coulter) or Cytek Northern Lights (Cytek Biosciences, Fremont, CA), and data were analyzed with FlowJo software (Tree Star). Histology For

(17) Distinct molecular profiles of skull bone marrow in health and neurological disordersCellAugust 17, 2023Zeynep Ilgin Kolabas, Louis B. Kuemmerle, Robert Perneczky, Benjamin Förstera, Selin Ulukaya, Mayar Ali, Saketh Kapoor, Laura M. Bartos, Maren Büttner, Ozum Sehnaz Caliskan, et al.47-0193-82). Cells were washed with FACS buffer, resuspended in 200 μl of FACS buffer and acquired using a Cytek® Northern Lights (Cytek® Biosciences, US) and analyzed using FlowJo software (Treestar, US). Isotype controls were used

(18) Longitudinal analysis of memory TFH cells and antibody response following CoronaVac vaccinationJCI InsightAugust 8, 2023Pengcheng Zhou, Cheng Cao, Tuo Ji, Ting Zheng, Yaping Dai, Min Liu, Junfeng Jiang, Daoqi Sun, Zhonghu Bai, Xiaojie Lu, et al.acquisition. Detailed immune phenotyping of CD4+ T cells using 24-color flow cytometry was performed on Cytek Northern Lights with standardized configuration. Dead cells were routinely excluded from the analysis by staining with

(19) The co-delivery of adenovirus-based immune checkpoint vaccine elicits a potent anti-tumor effect in renal carcinomanpj VaccinesAugust 4, 2023Nan Jiang, Yanyan Zheng, Jiage Ding, Jiawei Wang, Fei Zhu, Meng Wang, Navid Sobhani, Praveen Neeli, Gang Wang, Hailong Li, et al.(Biolegend, Cat. 503808, 1:100). All data were obtained on a BD FACSCanto II (BD Biosciences) or Cytek® Northern Lights (Cytek Biosciences) and analyzed using FlowJo software (Tree Star Inc.). Detection of CD8 T-cell proliferation Lymphocytes

(20) AI-powered discovery of a novel p53-Y220C reactivatorFront OncolAugust 1, 2023Shan Zhou, Dafei Chai, Xu Wang, Praveen Neeli, Xinfang Yu, Aram Davtyan, Ken Young, Yong Liwere stained with 7-amino-actinomycin D (7-AAD; BioLegend, San Diego, CA, USA) and analyzed using a Northern Lights flow cytometer (Cytek Biosciences, Fremont, CA, USA). Apoptosis analysis Apoptosis of BxPC-3, NUGC-3,

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