ID7000™ Spectral Cell Analyzer from Sony Corporation

Product Specs
ItemID7000™ Spectral Cell Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberID7000
Detection32-channels (PMT arrays); can be configured with 186 detectors to detect 44 colors or more
Throughput40,000 events per second
Lasers3 to 7 lasers
Excitation320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
Parameters186 fluorescence channels
CapacityTubes (5 mL), Microplates using autosampler (96-well, 384-well)
Data Throughput40,000 events/sec
Dimensions(WxDxH) 106 x 71.9 x 76 cm
Flow Rate33 to 200 µL/min

(1) Efficient inference of non-polyreactive antibody variants dependent on local fine-tuningmAbsDecember 31, 2026Matthew Saunders, Sophie Comyn, Borka Medjo, Zahra Anderson, Chase LaCombe, Jake Ripley, Kyra Chapman, Tim Ma, Abigail Ventura, Alyssa Ruelos, et al.minutes, and washed once with ice‑cold PBS. Cell pellets were resuspended in 100 uL PBS and run on a SONY ID7000 spectral cell analyzer. All experiments were performed in biological triplicate and 100,000 events were analyzed using FlowJo

(2) A macrophage-mimetic nanocarrier co-loaded with geraniol and FK13-a1 for MRSA-induced acute lung injuryDrug DelivDecember 31, 2026Haonan Li, Binghong Xu, Junguang Liang, Tao Hu, Tingting Wu, Liqin Lai, Xuan Zou, Ziquan Lv, Xiangjie Yao, Xiaobao Jin, et al.CD4+ T cells, CD8+ T cells, neutrophils, monocyte/macrophages, and Tregs were detected using a Sony ID7000 flow cytometer. 2.22. Statistical analysis All data are presented asmean ± standard deviation (SD)

(3) Oncolytic virus-delivered GSDME: Unlocking pyroptosis to improve dendritic cell function and amplify cytotoxic T cell responseMolecular Therapy OncologySeptember 17, 2026Sabrina Schneider, Theresa Schwaiger, Joelle Kröll, Ioannes Basbas, Fabian Martin, Dongyue Xin, Elke Podgorschek, Ilona Arnkil, Marlies Glatz, Melissa Mayr, et al.suspension and cells were analyzed on a MacsQuant16 (Miltenyi Biotec) conventional flow cytometer or on a ID7000 Spectral Cell Analyzer (Sony Biotechnology). Data were analyzed using FlowJo software and visualized using GraphPad Prism v.10.4.1. Ex

(4) GW4869 Depletes Macrophages and Increases Number of Extracellular Vesicles in Murine Peritoneal Cavity FluidJ Extracell BiolAugust 28, 2026Johann Mar Gudbergsson, Pedro Henrique Gobira, Rodrigo Grassi-Oliveira, Anders Etzerodt30 min, resuspended in FACS buffer + viability dye, and 300–400.000 events were recorded on a SONY ID7000 spectral flow cytometer using standardized mode. Reference spectra were made from antibody single stains

(5) Serological Predictors of Protection from Infection Upon Household Exposure to SARS-CoV-2Eur J ImmunolAugust 24, 2026Henrike Maaß, Imke Hinrichs, Martina Pavletic, Manuela Harries, Tatjana Prinke, Najat Bdeir, Richard Egelkamp, Berit Lange, Yannic C. Bartsch, Mate Lerga, et al.HydroSpeed automated plate washer (Tecan, Switzerland), resuspended in assay buffer, and acquired at the ID7000 Spectral Cell Analyzer (Sony Biotechnology, CA, USA). 4.8 Biotinylation and Bead‐Coupling of Antigens For antibody‐dependent

(6) AI-enabled discovery and biochemical optimization of minibinders targeting cancer cell-surface proteinsNat CommunAugust 20, 2026Bianca Broske, Benjamin A. McEnroe, Sophie C. Frechen, Tim N. Kempchen, Caroline I. Fandrey, Elisabeth Tan, Dominic Ferber, Michelle C. R. Yong, Marie Kleinert, Julia M. Messmer, et al.Biolegend). Samples were acquired using the 4-Laser Cytek Aurora Spectral Flow Cytometer or 5-Laser Sony ID7000 Spectral Cell Analyzer, and analyzed using FlowJo software. CAR-T cell activation assay CAR-T cells were plated with indicated

(7) Miconazole as a Repurposed Anticancer Candidate for Prostate CancerACS OmegaAugust 11, 2026Eswara N. H. K. Ghali, Lindsey Shim, Rajasekhar Baru, Anupam Dhasmana, Ryan J. Chang, Sung Yun Jung, Rahul Tiwari, Neeraj Chauhan, Vivek Kashyap, Sheema Khan, et al.room temperature in the dark. Finally, cells were analyzed (Ex (Max): 535 nm, Em (Max): 617 nm) using ID7000 spectral cell analyzer, Flow cytometer (SONY, USA). Data were analyzed with the ID7000 system software, and cell populations

(8) Impact of intranasal cigarette smoke exposure on murine peripheral immune responses and lung pathologyFront ToxicolAugust 3, 2026Laura G. Bartlett, Yasmin Azizbayli, Amanda L. Tatler, Adam J. Watkins, Lucy C. Faircloughimmune cell populations were analysed by multiparametric flow cytometry. Data was acquired on a Sony ID7000 flow cytometer at the University of Nottingham Flow Cytometry Facility. A viability dye was used to

(9) Induced pluripotent stem cells from sporadic pulmonary veno-occlusive disease exhibit altered endothelial commitment and stress-related transcriptional programsFront Cell Dev BiolAugust 3, 2026Ling Lian, Noah Daniels, Nicholas Wanner, Suzy Comhair, Gunnar H. D. Poplawski, Kewal Asosingh, Rodrigo Lopez-Gonzalez(Thermo Fisher, 00-5523-00) according to the manufacturer’s instructions. Data were acquired using a Sony ID7000 spectral flow cytometer equipped with six lasers. Spectral unmixing was performed using UltraComp eBeads

(10) A Microgel-Based Platform for Tunable Expansion and Function of γδ T-cellsbioRxivJuly 24, 2026Favour Omafuvwe Obuseh, Junzhe Lou, Michelle Chang, Anqi Chen, David Weitz, David Mooneyperformed per manufacturer’s instructions. Flow cytometry acquisition was carried out with the Sony ID7000 spectral cytometer. Gating was performed based on fluorescence-minus-one controls after compensation

(11) Viscoelastic Niches Shape γδ T-Cell Phenotype and Effector FunctionbioRxivJuly 24, 2026Favour Omafuvwe Obuseh, Michelle Chang, Joshua Price, Kyle Ruark, Tania To, Leah Lourenco, Bogdan Budnik, David Mooneyperformed per manufacturer’s instructions. Flow cytometry acquisition was carried out with the Sony ID7000 spectral cytometer. Gating was performed based on fluorescence-minus-one controls after compensation

(12) Hormonal Contraceptive Use and Musculoskeletal Injury Risk in Female Athletes: A Prospective Cohort StudySports HealthJuly 23, 2026Natasha Trentacosta, Giselle Kaneda, Natasha Schimmoeller, Dave T. Huang, Jasmine Galloway, Marilee Fisher, Chloe Castaneda, Christian Blough, Thomas Kremen, Caroline Jefferies, et al.buffer and run according to manufacturer protocol. Fluorescent intensity was acquired using a Sony ID7000 Spectral Cell Analyzer (Sony). The resulting data were analyzed using the online LEGENDplex analysis software. Injury Tracking Injury

(13) Intratumoral TIGIT blockade augments antitumor responses and bypasses increased functionality of peripheral TIGIT+ NK cells in mouse and human models of bone and soft tissue sarcomaJ Immunother CancerJuly 22, 2026Marshall L Lammers, Cyrus J Sholevar, Makan Karimzadeh, Aryana M Razmara, Sylvia M Cruz, Megan C Purl, Natalie M Liu, Danika Bakke, Rosemary N Plagens, Andrew Elliott, et al.and intracellular targets.13 31–33 Flow cytometry was performed using either BD LSRFortessa or Sony ID7000 analyzer. A complete list of flow cytometry antibodies is available in online supplemental table 1.

(14) Heparan Sulfate Controls Nanoscale Assembly of GPC3-Wnt Receptor ComplexesbioRxivJuly 22, 2026Shaoli Lin, David A. Ball, Mohamadreza Fazel, Tatiana S. Karpova, Mitchell HoAllophycocyanin (Jackson ImmunoResearch, Cat#711-116-152) at a dilution of 1:200. Data was visualized using SONY ID7000 (Sony Biotechnology) and analyzed by FlowJo v10.4.2 software (FlowJo, LLC). Confocal Microscopy For

(15) Protocol for deep phenotyping of human antigen-specific T cells across conventional and unconventional subsets by 22-color spectral flow cytometrySTAR ProtocJuly 15, 2026Jiahui Du, Mingzhe Zeng, Ziqian He, Xiang Li, Shuxun Liu, Zhiqing LiμL of PBS per well.i.Proceed with spectral analyzer setup and sample acquisition.10.Set up the Sony ID7000 spectral cell analyzer. Perform fluidics priming, flow cell purge, and QC calibrations, following the manufacturer’s instructions.

(16) Multiome-based identification of molecular markers for prospective identification of platelet-biased HSCsStem Cell ReportsJuly 14, 2026Bowen Zhang, Yiran Meng, Esther Rodríguez Correa, Xiying Ren, Alexandre Fagnan, Michael D. Milsom, Claus Nerlov-cell transplantation and single-cell qPCR experiments. Spectral flow cytometry was performed using a Sony ID7000 spectral analyser. Single-colour reference controls were prepared using UltraComp eBeads (ThermoFisher,

(17) Engineering trispecific IL-2 receptor agonistic antibodies through geometry optimization for enhanced Treg targetingNature CommunicationsJuly 10, 2026Valentina Lykhopiy, Emilie Pollenus, Laurie Rangan, Michelle Stakenborg, Tugsan Tezil, Michiel Varheust, Giel Tanghe, Nina Lambrechts, Vanshika Malviya, Eva De Langhe, et al.staining, counting beads (Spherotech) were added to each sample. Samples were acquired using a Sony ID7000 spectral flow cytometer (Sony Biotechnology Inc.) and analyzed with FCS Express software (version 7,

(18) Inhalable ROS-Responsive Nanospray Activates PPAR-γ to Restore Macrophage Mitochondrial Homeostasis and Attenuate Radiation-Induced Lung InjuryAdv Sci (Weinh)July 8, 2026Mingquan Gao, Xudong Yu, Ziqian Shang, Mengyao Tan, Xie Huang, Zaizhi Du, Xiaojiao Wang, Xinrui Yang, Ximei Luo, Weidong Wang, et al.catalog numbers, and dilutions) is provided in Table S1. Following washing, data were acquired on a Sony ID7000 spectral flow cytometer. After excluding debris and doublets, macrophages were defined by the CD45+CD11b+F4/80+

(19) An optimized method to differentiate HL60 cells into neutrophil-like cellsJournal of Leukocyte BiologyJuly 3, 2026Samuel P Collie, Grant Yu, Suraj S Rawat, Catharina Rypstra, Carole A Parenttransferred to flow cytometry tubes, and analyzed immediately. Data was acquired on either LSR Fortessa, Sony ID7000, Sony SH800, Bio-Rad Ze#5, or Beckman Coulter CytoFLEX S Flow cytometers, recording 100,000 events per

(20) The mitochondrial unfolded protein response in human microglia disrupts neuronal–glial communication and promotes senescenceNature NeuroscienceJune 26, 2026Maria Jose Perez J, Alicia Lam, Christin Weissleder, Federico Bertoli, Hariam Raji, Mariella Bosch, Ivan Nemazanyy, Stefanie Kalb, Mohammed Kehili, Insa Hirschberg, et al.measured with the Human Essential Immune Response Panel (BioLegend GmbH, cat. no. 740930) and a Sony ID7000 spectral cell analyzer (Sony Biotechnology). Data (>300 beads per sample) were analyzed with LEGENDplex software v9.0 (BioLegend,

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ID7000™ Spectral Cell Analyzer

ID7000™ Spectral Cell Analyzer from Sony Corporation

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ID7000™ Spectral Cell Analyzer from Sony Corporation

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