Metabolite assay kits enable the rapid and reliable analytical measurements of bioactive small molecules in biological samples such as cells, tissues, blood, and bodily fluids. These kits, which generally contain the necessary reagents in a ready-to-use format, can serve as useful tools for many research applications. These include studying metabolic pathways, measuring disease biomarkers, assessing pharmacological effects, toxicology, and investigating cellular processes. Many metabolite assays rely on the generation of a colored or fluorescent byproduct. This can arise from a direct reaction with a specific probe, or via an enzyme-coupled reaction. An example is the oxidation of glucose by glucose oxidase to produce hydrogen peroxide, which subsequently reacts with another compound to produce a color change. Colorimetric or fluorometric detection is generally carried out using a plate reader or spectrophotometer to quantify the target metabolite. Explore our catalog of assay kits for common metabolite molecules sourced by many reliable suppliers.
A guide to viability, cytotoxicity, proliferation, and apoptosis
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Tips to help identify an approach that best meets experimental needs
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I want to investigate the genetic connection between cell wall hydrolase knockouts of bacteria and cell wall-acting antibiotic vancomycin. Therefore, I have used this product to examine the vancomycin sensitivity of these mutant bacteria.
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This kit was used to quantify SA-ß-gal levels in proliferative and irradiated human brain microvascular endothelial cells (HBMECs), according to manufacturer's instructions. We also used in different vascular cells (HUAECs, bENd.3 cells).
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