Metabolite assay kits enable the rapid and reliable analytical measurements of bioactive small molecules in biological samples such as cells, tissues, blood, and bodily fluids. These kits, which generally contain the necessary reagents in a ready-to-use format, can serve as useful tools for many research applications. These include studying metabolic pathways, measuring disease biomarkers, assessing pharmacological effects, toxicology, and investigating cellular processes. Many metabolite assays rely on the generation of a colored or fluorescent byproduct. This can arise from a direct reaction with a specific probe, or via an enzyme-coupled reaction. An example is the oxidation of glucose by glucose oxidase to produce hydrogen peroxide, which subsequently reacts with another compound to produce a color change. Colorimetric or fluorometric detection is generally carried out using a plate reader or spectrophotometer to quantify the target metabolite. Explore our catalog of assay kits for common metabolite molecules sourced by many reliable suppliers.
Expanding suite of tools and technologies is advancing the field
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Both options have value and can complement one another
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I want to investigate the genetic connection between cell wall hydrolase knockouts of bacteria and cell wall-acting antibiotic vancomycin. Therefore, I have used this product to examine the vancomycin sensitivity of these mutant bacteria.
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I study gene signaling pathways and routinely measure cell viability and responses to experimental treatments. CellTiter-Glo 2.0 provides a simple luminescence-based readout of ATP and is convenient for multiwell experiments
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