Isocitrate Assay Kits

Isocitrate assay kits include essential reagents to measure the abundance or concentration of isocitrate in biological samples. Isocitrate, a key intermediate in the Krebs cycle, is formed from citrate by aconitase and subsequently converted into a-ketoglutarate by isocitrate dehydrogenase. Isocitrate assays that utilize the enzymatic oxidation of isocitrate produce the byproduct NADPH, which reacts with a colorimetric dye or fluorescent probe. The generated signal is proportional to the concentration of isocitrate in the sample. Isocitrate assays can be used in investigating mitochondrial function, energy production, and regulation of the TCA cycle, as well as in studying other isocitrate-related processes in the context of cancer and neurodegenerative diseases. When choosing an assay kit, consider important specifications such as the assay detection range, recommended sample types, and the components included within the kit. Visit the supplier page for more details.