MojoSort™ Mouse CD8 T Cell Isolation Kit 480035 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD8 T Cell Isolation Kit
CompanyBioLegend
Price $654.00Supplier Page View Company Product Page
Catalog Number480035
Quantity200 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD8
Cell Type (standardized)CD8+ T Cell

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Contrasting functions of CD73 and adenosine in CD8+ T-cell exhaustion during antitumor immunityOncoimmunologyDecember 31, 2026Juan Saavedra-Almarza, Solange Gouët, Felipe Malgue, Catalina Bascuñan, Andrés Hernández-Oliveras, Joaquín De la Rosa, Javiera Reyes-Alvarez, Camilo Villaman, José Corrales-Bermúdez, Justine Castañeda, et al.magnetically isolated from the spleen of OT-I mice using the MojoSort Mouse CD8 T Cell Isolation Kit (cat: 480035, Biolegend) or the EasySep Mouse Naïve CD8+ T Cell Isolation Kit (cat: 19858, STEMCELL Technologies).

(2) Sulfur partitioning from cysteine controls T cell proliferation and effector functionCellMarch 31, 2026Beth Kelly, Minsun Cha, Tatjana Gremelspacher, Jacob L. Martin, Massimo Andreis, Isha Maloo, Gustavo E. Carrizo, Mia Gidley, Michal A. Stanczak, Petya Apostolova, et al.BODIPY 581/591 C11 (Lipid peroxidation sensor),Invitrogen,Cat# D3861 MojoSort mouse CD8 T cell isolation kit,Biolegend,Cat# 480035 Qubit RNA Broad Range assay kit,Thermo Fisher Scientific,Cat# Q10211 Qiagen RNeasy mini kit,Qiagen,Cat#

(3) Spatial tumor evolution panorama of ovarian cancerCell Rep MedMarch 17, 2026Chenzhao Feng, Yunyi Yang, Guanchu Li, Zixuan Fang, Bin Yang, Fang Zhao, Xingyu Chen, Shuyi Liu, Di Wu, Xingyuan Hu, et al.erythrocytes. Subsequently, T cells were isolated using the Mouse CD8 T cell Isolation Kit (BioLegend, 480035). Isolated T cells were cultured in T cell medium composed of RPMI-1640 medium supplemented with L-glutamine,

(4) Hypoxia-driven remodeling of SELENOP+ macrophages shapes T cell dynamics and promotes ovarian cancer metastasisNature CommunicationsJanuary 12, 2026Qing Liu, Chenzhao Feng, Tianhao Wu, Siyang Zhang, Xinyi Wang, Qian Zhao, Xueying Song, Shuangyan Liu, Linru Quan, Yuli Zhang, et al.human CD8+ T cells of HGSOC patients were isolated by the MojoSort™ Mouse CD8 T Cell Isolation Kit (Cat#480035, Biolegend) and MojoSort™ Human CD8 T Cell Isolation Kit (Cat#480129, Biolegend), and subsequently stimulated

(5) DAPK1-positve macrophages facilitate immunosuppressive microenvironment and determine immunotherapy efficacy in colorectal cancerJournal of Translational MedicineNovember 4, 2025Jiang Chang, Yuxu Niu, Shizhao Zhou, Ziqi Zhang, Weiying Zhu, Junchang Zhu, Haoran Xiu, Ke Shang, Qingyang Feng, Ye Weidifferentiation. Co-culture assay CD8+T cells were isolated from C57BL/6 mouse spleens via magnetic bead separation (480035, BioLegend). Following purification, cells were enumerated and seeded into 96-well plates pre-coated

(6) The transcriptional repressor BLIMP1 enforces TCF-1-dependent and -independent restriction of the memory fate of CD8+ T cellsImmunityOctober 14, 2025Maegan K. Murphy, Matthew McCullen, Joshua L. Deffenbaugh, Andy Y. Chen, Joy Pai, Bence Daniel, Amir Yousif, Saravanan Raju, Sunnie Hsiung, Zhenxiao Wang, et al.WT P14 or Tcf7Δ+22kb P14 mice using the MojoSort CD8 T cell negative enrichment kit (Biolegend, Cat# 480035) according to the manufacturer protocol and washed twice in PBS. Cell pellets were resuspended to a

(7) DPP7 promotes fatty acid β-oxidation in tumor-associated macrophages and determines immunosuppressive microenvironment in colorectal cancerInternational Journal of Biological SciencesOctober 1, 2025Jiang Chang, Yuxu Niu, Shizhao Zhou, Weiying Zhu, Ziqi Zhang, Haoran Xiu, Ke Shang, Qingyang Feng, Ye Weiwere isolated from the spleens of wild-type C57BL/6 mice using a magnetic bead-based isolation kit (480035, BioLegend). Purified cells were quantified and plated in anti-CD3/CD28 pre-coated (2μg/mL, 100340 BioLegend,

(8) Activated T cells break tumor immunosuppression by macrophage re-educationCancer DiscoveryJuly 3, 2025Rosa Trotta, Silvia Rivis, Shikang Zhao, Marie-Pauline Orban, Sarah Trusso Cafarello, Iris Charatsidou, Joanna Pozniak, Jonas Dehairs, Lotte Vanheer, Carlos A. Pulido Vicuna, et al.R7757). Naïve CD8+ and CD4+ T cells were isolated using the mouse CD8+ T-cell Isolation Kit (BioLegend, 480035) or the mouse CD4+ T-cell Isolation Kit (BioLegend, 480033) according to the manufacturer’s instructions.

(9) Macrophages foster anti-tumor immunity by ZEB1-dependent cytotoxic T cell chemoattractionCommun BiolJuly 1, 2025Kathrin Fuchs, Elisabetta D'Avanzo, Isabell Armstark, Ruthger van Roey, Ana Clavel Ezquerra, Nino Bindel, Katharina Siebenkäs, Yussuf Hajjaj, Renato Liguori, Fulvia Ferrazzi, et al.buffer and enriched for CD8 + T cells using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufacturer´s instructions. For the T cell attraction assays, 500 μl of double concentrated

(10) Antisense oligonucleotides targeting IRF4 alleviate psoriasisActa Pharmaceutica Sinica BMay 14, 2025Yanxia Yu, Yirui Wang, Weiwei Chen, Chang Zhang, Zhuo li, Jing Yu, Minhao Wang, Can Song, Sihao Yan, Jiayi Lu, et al.F4/80+ cell sorting. CD4 nanobeads (#480070, 20 μL; BioLegend), a mouse CD8 T cell isolation kit (#480035; BioLegend), mouse CD19 nanobeads (#480002; BioLegend), and mouse CD11c nanobeads (#480078; BioLegend)

(11) Immunometabolite L-2-HG promotes epigenetic modification of exhausted T cells and improves anti-tumor immunityJCI InsightMarch 4, 2025Yanying Yang, Xiaoyan Li, Fangming Liu, Mingyue Ma, Ying Yang, Chengchao Ruan, Yan Lu, Xiaoyang Li, Xiangdong Wang, Yinghong Shi, et al.cells were isolated from the splenocytes using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, 480035). The sorted OT-1 CD8+ T cells were treated with L-2-HG (300 μM; Toronto Research Chemicals, H942596)

(12) NFAT single-deficient murine T cells reduce the risk of aGvHD while controlling cytomegalovirus infectioniScienceFebruary 21, 2025Nadine Hundhausen, Snigdha Majumder, Yin Xiao, Sigrun S. Haeusl, Helen Goehler, Rishav Seal, Cristina M. Chiarolla, Andreas Rosenwald, Matthias Eyrich, Luka Cicin-Sain, et al.instructions. CD4+ or CD8+ T cells were subsequently isolated using the negative Isolation kit (480033 or 480035, Biolegend) according to the manufacturer’s instructions.3 Allogenic hematopoietic stem cell transplantation CB6F1

(13) VSIG4+ tumor-associated macrophages mediate neutrophil infiltration and impair antigen-specific immunity in aggressive cancers through epigenetic regulation of SPP1J Exp Clin Cancer ResFebruary 7, 2025Zongfu Pan, Jinming Chen, Tong Xu, Anqi Cai, Bing Han, Ying Li, Ziwen Fang, Dingyi Yu, Shanshan Wang, Junyu Zhou, et al.room temperature for 5 min and centrifuged at 300 × g for 5 min. A CD8+ T cell sorting kit (Biolegend, 480035) was used for CD8+ T cell sorting under sterile conditions according to the manufacturer’s instructions.

(14) Aging impairs CD8 T cell responses in adoptive T-cell therapy against solid tumorsFrontiers in ImmunologyJanuary 24, 2025Gulfiya Kadyrzhanova, Miho Tamai, Shukla Sarkar, Rajkumar Singh Kalra, Hiroki Ishikawamin. CD8 T cells were negatively selected using a MojoSort Mouse CD8 T Cell Isolation Kit (Biolegend, 480035). CD8 T cells were then resuspended in complete RPMI-1640 media containing 2 mM L-glutamine, 1% HEPES

(15) Imaging of biphasic signalosomes constructed by checkpoint receptor 2B4 in conventional and chimeric antigen receptor-T cellsiScienceJanuary 17, 2025Ryohei Matsushima, Ei Wakamatsu, Hiroaki Machiyama, Wataru Nishi, Yosuke Yoshida, Tetsushi Nishikawa, Hiroko Toyota, Masae Furuhata, Hitoshi Nishijima, Arata Takeuchi, et al.assays ,, Mojo Mouse CD4 T Cells Isolation Kit,BioLegend,480033 Mojo Mouse CD8 T Cells Isolation Kit,BioLegend,480035 APC Annexin V Apoptosis Detection Kit with 7-AAD,BioLegend,640930 TNF-α ELISA Kit,BioLegend,430901 "CD19 MicroBeads, mouse

(16) CCR2 restricts IFN-γ production by hippocampal CD8 TRM cells that impair learning and memory during recovery from WNV encephalitisJournal of NeuroinflammationDecember 27, 2024Shenjian Ai, Artem Arutyunov, Joshua Liu, Jeremy D. Hill, Xiaoping Jiang, Robyn S. KleinPercoll gradient centrifugation using MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, cat. no. 480035). For Cre− and Cre+ mice, forebrain tissues were collected following cardiac perfusion. RNA was isolated

(17) Interleukin-1α release during necrotic-like cell death generates myeloid-driven immunosuppression that restricts anti-tumor immunityCancer cellDecember 9, 2024Hänggi K, Li J, Gangadharan A, Liu X, Celias DP, Osunmakinde O, Keske A, Davis J, Ahmad F, Giron A, et al.CellTrace™ Violet Cell Proliferation Kit,ThermoFisher,Cat# C34557 MojoSort Mouse CD8+ T cell Isolation Kit,Biolegend,Cat# 480035 In-Fusion® Snap Assembly Master Mix with Competent Cells,TakaraBio,Cat# 638951 Nucleospin PCR/Gel

(18) Acetylcysteine synergizes PD-1 blockers against colorectal cancer progression by promoting TCF1+PD1+CD8+ T cell differentiationCell Communication and SignalingOctober 17, 2024Wenchang Zhou, Mengdi Qu, Ying Yue, Ziwen Zhong, Ke Nan, Xingfeng Sun, Qichao Wu, Jie Zhang, Wankun Chen, Changhong Miaospleens were surgically removed. Subsequently, we used a MojoSort™ mouse CD8 T Cell isolation kit (Cat # 480035, Biolegend), a negative screening method, to isolate CD8+ T cells from the spleen. Following spleen

(19) Activation of NOD1 on tumor-associated macrophages augments CD8(+) T cell-mediated antitumor immunity in hepatocellular carcinomaScience advancesOctober 4, 2024Zhang F, Jiang Q, Cai J, Meng F, Tang W, Liu Z, Lin X, Liu W, Zhou Y, Shen X, et al.isolated from the spleens of wild-type C57BL/6 mice using the CD8 T Cell Isolation Kit (BioLegend, #480035) after red blood cell lysis. For the proliferation assay, purified CD8+ T cells were labeled with 2

(20) RIG-I is an intracellular checkpoint that limits CD8+ T-cell antitumour immunityEMBO Molecular MedicineSeptember 25, 2024Xiaobing Duan, Jiali Hu, Yuncong Zhang, Xiaoguang Zhao, Mingqi Yang, Taoping Sun, Siya Liu, Xin Chen, Juan Feng, Wenting Li, et al.cells were further purified using the MojoSort™ Mouse CD8+ T-cell isolation kit (BioLegend, 480044 and 480035). After stimulation with PMA (100 ng/ml), ionomycin (2 μg/ml) and GolgiStop (1:1500 dilution) for 4

Write a Review

(1)

11/16/2020Great Murine CD8 Isolation Kit Meng SunThe isolation is 90% clean and CD8s are always enriched.
(1)

1/10/2020Good Isolation Kit to Recover Untouched Mouse CD8+ T CellsDr. Min Hee OhWe are very happy in the end using this kit since it's very cheap per isolation. Yield and purity is very acceptable especially with transgenic mouse T cells.
(1)

1/6/2020Good BioLegend Mouse CD8 T Cells Isolation Kit Wei XuYou can use this kit to get acceptable yield and purity to isolate CD8 cells from mouse with reasonable price.
(1)

6/18/2019Easy-to-Use Isolation Kit Meng SunThis kit is a great alternative to other costly brands. The purity is great, the yield is good, and changing to this brand has cut our lab's cost by a few thousands of dollar a year.

MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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