MojoSort™ Mouse CD8 T Cell Isolation Kit 480035 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD8 T Cell Isolation Kit
CompanyBioLegend
Price $654.00Supplier Page View Company Product Page
Catalog Number480035
Quantity200 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD8
Cell Type (standardized)CD8+ T Cell

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Contrasting functions of CD73 and adenosine in CD8+ T-cell exhaustion during antitumor immunityOncoimmunologyDecember 31, 2026Juan Saavedra-Almarza, Solange Gouët, Felipe Malgue, Catalina Bascuñan, Andrés Hernández-Oliveras, Joaquín De la Rosa, Javiera Reyes-Alvarez, Camilo Villaman, José Corrales-Bermúdez, Justine Castañeda, et al.magnetically isolated from the spleen of OT-I mice using the MojoSort Mouse CD8 T Cell Isolation Kit (cat: 480035, Biolegend) or the EasySep Mouse Naïve CD8+ T Cell Isolation Kit (cat: 19858, STEMCELL Technologies).

(2) Single-cell genomics links targeted functional manipulations to efficacy-associated chromatin signatures in CAR T cellsResearch SquareJune 29, 2026Kole R. Scott-Browne, Stephanie DeGolier, Marwan. A. Abushawish, Marwan Abushawish, Terry J. Prete, James P. Scott-Browneold C57BL/6 mouse splenocytes using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, Cat No. 480035). On day 0, T cells were activated with 1μg/mL anti-CD3 and 1μg/mL anti-CD28 overnight. Cells were transduced

(3) Depletion of NAT10 in T cells attenuates metabolic dysfunction–associated steatohepatitis in miceHepatol CommunJune 12, 2026Yanying Yang, Jie Lu, Mingyue Ma, Jing Zhou, E. Wang, Yan Lu, Zhe Dai, Bing Zhou, Yao LiMojoSort Mouse CD3 T Cell Isolation Kit (BioLegend, 480024) and the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), respectively, strictly following the manufacturer’s protocols. Flow cytometry Mouse livers were

(4) Targeting tumor-specific T cells with LAG3-directed interleukin-2 prevents T-cell exhaustion and reinvigorates antitumor immunitySignal Transduct Target TherJune 2, 2026Xiaohong Yu, Huiping Liao, Jiaoyun Lv, Yu Sun, Ruiqi Zhang, Yiwei Chen, Yifan Lin, Lulu Liu, Shijie Li, Hui Tang, et al.from tumors from LAG3-InFc-LaIL2- or control-treated mice via magnetic bead purification (BioLegend, 480035). To assess CTL-mediated cytotoxicity, CD8+ T cells (2.5e6, effector) and MC38 tumor cells (5e5, target)

(5) Ezh2 Inhibits the Differentiation of Short‐Lived Effector CD8 + T Cells and Promotes T Cell‐Dependent Antitumor ImmunityCancer ScienceJune 2, 2026Kenji Takagi, Junpei Suzuki, Yuko Matsuoka, Makoto Kuwahara, Taro Oshikiri, Masakatsu YamashitaMenin KO, Ezh2 KO, WT OT‐1 Tg, or Ezh2 KO OT‐1 Tg mice using a MojoSort CD8+ T Cell Isolation Kit (cat# 480035; BioLegend, CA, USA) with biotin anti‐CD44 mAb (cat# 103004; BioLegend) using autoMACS pro. The purified

(6) Macrophage-derived fibronectin suppresses antitumor immunity via tissue stiffening and immunosuppressive cell induction in cancer mouse modelsNature CommunicationsMay 22, 2026Aitian Li, Ying Wang, Haiqing Bai, Xin Xie, Beibei Xu, Yunhan Wang, Shengjun Luo, Lei Zhang, Qitai Zhao, Shichao Duan, et al.T cells were dissociated from spleens using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, 480035) through magnetic cell sorting. The human monocyte THP-1 cell line (National Collection of Authenticated

(7) ETS1 Orchestrates a Hybrid EMT Program Driving Metastasis and Immune EvasionCancer ResMay 19, 2026Benjamin Ziman, Talia A. Wenger, Chehyun Nam, Qian Yang, Daniel Arnaudov, Thomas Tilton, Megha Sheth, Zhixuan Jing, Yuhao Pan, Joseph A. Vargas, et al.hours. Next, CD8+ T Cells were isolated using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), counted, and resuspended in RPMI-1640 media containing 20% FBS. Media from MOC2 cells was removed

(8) Age-related reduction of pyruvate dehydrogenase kinase 1 impairs T cell responsesFront ImmunolMay 11, 2026Rajkumar S. Kalra, Miho Tamai, Shukla Sarkar, Yong-Woon Han, Mio Miyagi, Daiki Sasaki, Hiroki Ishikawatotal CD4+ and CD8+ T cell purification, MojoSort CD4+ and CD8+ specific selection kits (#480033 and #480035, respectively) were used. The purity of cells (93-97%) was confirmed by FACS Fortessa (BD). Purified

(9) Hypoxia Impairs CD8+ T Cell Fitness and Is Associated with a Dysfunctional CD8+ T Cell State in Pancreatic CancerCancers (Basel)May 8, 2026Ashley M. Mello, Marina Pasca di Magliano, Kyoung Eun Leespleens of C57BL/6 mice (#000664, Jackson Laboratory) using the MojoSort Mouse CD8 T Cell Isolation Kit (#480035, BioLegend, San Diego, CA, USA). Cells were activated for 24 h using plate-bound anti-CD3 (145-2C11,

(10) ERα blockade in dendritic cells enhances antigen cross-presentation and induces antitumor CD8+ T cell immunityNature CommunicationsMay 5, 2026Yaxin Liu, Zhongfei Ma, Xuelian Li, Yuanyuan Ding, Zhiqing Li, Bin Zhang, Yumei Zhou, Jun Zhou, Shuo Liu, Shuxun Liu, et al.cell sorter. Splenic CD8+ T cells were isolated with the MojoSort™ Mouse CD8 T Cell Isolation Kit (480035; BioLegend) according to the manufacturer’s protocol. Isolation of murine splenic cDC1 and cDC2 subsets Splenic

(11) Sulfur partitioning from cysteine controls T cell proliferation and effector functionCellMarch 31, 2026Beth Kelly, Minsun Cha, Tatjana Gremelspacher, Jacob L. Martin, Massimo Andreis, Isha Maloo, Gustavo E. Carrizo, Mia Gidley, Michal A. Stanczak, Petya Apostolova, et al.BODIPY 581/591 C11 (Lipid peroxidation sensor),Invitrogen,Cat# D3861 MojoSort mouse CD8 T cell isolation kit,Biolegend,Cat# 480035 Qubit RNA Broad Range assay kit,Thermo Fisher Scientific,Cat# Q10211 Qiagen RNeasy mini kit,Qiagen,Cat#

(12) Spatial tumor evolution panorama of ovarian cancerCell Rep MedMarch 17, 2026Chenzhao Feng, Yunyi Yang, Guanchu Li, Zixuan Fang, Bin Yang, Fang Zhao, Xingyu Chen, Shuyi Liu, Di Wu, Xingyuan Hu, et al.erythrocytes. Subsequently, T cells were isolated using the Mouse CD8 T cell Isolation Kit (BioLegend, 480035). Isolated T cells were cultured in T cell medium composed of RPMI-1640 medium supplemented with L-glutamine,

(13) Contrasting functions of CD73 and adenosine in CD8+ T-cell exhaustion during antitumor immunityOncoimmunologyMarch 13, 2026Juan Saavedra-Almarza, Solange Gouët, Felipe Malgue, Catalina Bascuñan, Andrés Hernández-Oliveras, Joaquín De la Rosa, Javiera Reyes-Alvarez, Camilo Villaman, José Corrales-Bermúdez, Justine Castañeda, et al.magnetically isolated from the spleen of OT-I mice using the MojoSort Mouse CD8 T Cell Isolation Kit (cat: 480035, Biolegend) or the EasySep Mouse Naïve CD8+ T Cell Isolation Kit (cat: 19858, STEMCELL Technologies).

(14) Hypoxia-driven remodeling of SELENOP+ macrophages shapes T cell dynamics and promotes ovarian cancer metastasisNature CommunicationsJanuary 12, 2026Qing Liu, Chenzhao Feng, Tianhao Wu, Siyang Zhang, Xinyi Wang, Qian Zhao, Xueying Song, Shuangyan Liu, Linru Quan, Yuli Zhang, et al.human CD8+ T cells of HGSOC patients were isolated by the MojoSort™ Mouse CD8 T Cell Isolation Kit (Cat#480035, Biolegend) and MojoSort™ Human CD8 T Cell Isolation Kit (Cat#480129, Biolegend), and subsequently stimulated

(15) DAPK1-positve macrophages facilitate immunosuppressive microenvironment and determine immunotherapy efficacy in colorectal cancerJournal of Translational MedicineNovember 4, 2025Jiang Chang, Yuxu Niu, Shizhao Zhou, Ziqi Zhang, Weiying Zhu, Junchang Zhu, Haoran Xiu, Ke Shang, Qingyang Feng, Ye Weidifferentiation. Co-culture assay CD8+T cells were isolated from C57BL/6 mouse spleens via magnetic bead separation (480035, BioLegend). Following purification, cells were enumerated and seeded into 96-well plates pre-coated

(16) The transcriptional repressor BLIMP1 enforces TCF-1-dependent and -independent restriction of the memory fate of CD8+ T cellsImmunityOctober 14, 2025Maegan K. Murphy, Matthew McCullen, Joshua L. Deffenbaugh, Andy Y. Chen, Joy Pai, Bence Daniel, Amir Yousif, Saravanan Raju, Sunnie Hsiung, Zhenxiao Wang, et al.WT P14 or Tcf7Δ+22kb P14 mice using the MojoSort CD8 T cell negative enrichment kit (Biolegend, Cat# 480035) according to the manufacturer protocol and washed twice in PBS. Cell pellets were resuspended to a

(17) RTP4 Suppresses Colorectal Cancer Progression via MHC-I-Mediated CD8(+) T Cell Infiltration and Enhances Immunotherapy ResponseJournal of cellular and molecular medicineOctober 1, 2025Yu C, Li Y, Liu Z, Ye W, Wei L, Xu W, Yan L, Ye H.

(18) DPP7 promotes fatty acid β-oxidation in tumor-associated macrophages and determines immunosuppressive microenvironment in colorectal cancerInternational Journal of Biological SciencesOctober 1, 2025Jiang Chang, Yuxu Niu, Shizhao Zhou, Weiying Zhu, Ziqi Zhang, Haoran Xiu, Ke Shang, Qingyang Feng, Ye Weiwere isolated from the spleens of wild-type C57BL/6 mice using a magnetic bead-based isolation kit (480035, BioLegend). Purified cells were quantified and plated in anti-CD3/CD28 pre-coated (2μg/mL, 100340 BioLegend,

(19) Activated T cells break tumor immunosuppression by macrophage re-educationCancer DiscoveryJuly 3, 2025Rosa Trotta, Silvia Rivis, Shikang Zhao, Marie-Pauline Orban, Sarah Trusso Cafarello, Iris Charatsidou, Joanna Pozniak, Jonas Dehairs, Lotte Vanheer, Carlos A. Pulido Vicuna, et al.R7757). Naïve CD8+ and CD4+ T cells were isolated using the mouse CD8+ T-cell Isolation Kit (BioLegend, 480035) or the mouse CD4+ T-cell Isolation Kit (BioLegend, 480033) according to the manufacturer’s instructions.

(20) Macrophages foster anti-tumor immunity by ZEB1-dependent cytotoxic T cell chemoattractionCommun BiolJuly 1, 2025Kathrin Fuchs, Elisabetta D'Avanzo, Isabell Armstark, Ruthger van Roey, Ana Clavel Ezquerra, Nino Bindel, Katharina Siebenkäs, Yussuf Hajjaj, Renato Liguori, Fulvia Ferrazzi, et al.buffer and enriched for CD8 + T cells using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufacturer´s instructions. For the T cell attraction assays, 500 μl of double concentrated

Write a Review

(1)

11/16/2020Great Murine CD8 Isolation Kit Meng SunThe isolation is 90% clean and CD8s are always enriched.
(1)

1/10/2020Good Isolation Kit to Recover Untouched Mouse CD8+ T CellsDr. Min Hee OhWe are very happy in the end using this kit since it's very cheap per isolation. Yield and purity is very acceptable especially with transgenic mouse T cells.
(1)

1/6/2020Good BioLegend Mouse CD8 T Cells Isolation Kit Wei XuYou can use this kit to get acceptable yield and purity to isolate CD8 cells from mouse with reasonable price.
(1)

6/18/2019Easy-to-Use Isolation Kit Meng SunThis kit is a great alternative to other costly brands. The purity is great, the yield is good, and changing to this brand has cut our lab's cost by a few thousands of dollar a year.

MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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