BioLegend
Mouse CD8 T cell Isolation Kit
480035
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We use this kit to isolate CD8 T cells from mouse spleen and lymph node. We used to use Miltenyi kit for this purpose which is very expensive. After optimization of the amounts of antibody cocktail and beads to use, combined with Miltenyi LS columns and magnet, we're able to get good yield and purity.
CD8 T cell isolation from mouse
Mouse spleen and lymph node
Reduce the amount of beads a lot compared to what's said in the instructions
Need to optimize the amount of antibody cocktail and beads to get the best yield and purity. Columns and magnet help a lot.
N/A
So much cheaper the the Miltenyi kit
Yield is lower than Miltenyi kit; too much beads clog the column
You can use this kit to get acceptable yield and purity to isolate CD8 cells from mouse with reasonable price.