MojoSort™ Mouse CD8 T Cell Isolation Kit 480035 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD8 T Cell Isolation Kit
CompanyBioLegend
Price $654.00Supplier Page View Company Product Page
Catalog Number480035
Quantity200 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD8
Cell Type (standardized)CD8+ T Cell

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(21) Antisense oligonucleotides targeting IRF4 alleviate psoriasisActa Pharmaceutica Sinica BMay 14, 2025Yanxia Yu, Yirui Wang, Weiwei Chen, Chang Zhang, Zhuo li, Jing Yu, Minhao Wang, Can Song, Sihao Yan, Jiayi Lu, et al.F4/80+ cell sorting. CD4 nanobeads (#480070, 20 μL; BioLegend), a mouse CD8 T cell isolation kit (#480035; BioLegend), mouse CD19 nanobeads (#480002; BioLegend), and mouse CD11c nanobeads (#480078; BioLegend)

(22) Activated T Cells Break Tumor Immunosuppression by Macrophage ReeducationCancer DiscovApril 7, 2025Rosa Trotta, Silvia Rivis, Shikang Zhao, Marie-Pauline Orban, Sarah Trusso Cafarello, Iris Charatsidou, Joanna Pozniak, Jonas Dehairs, Lotte Vanheer, Carlos A. Pulido Vicuna, et al.R7757). Naïve CD8+ and CD4+ T cells were isolated using the mouse CD8+ T-cell Isolation Kit (BioLegend, 480035) or the mouse CD4+ T-cell Isolation Kit (BioLegend, 480033) according to the manufacturer’s instructions.

(23) Immunometabolite L-2-HG promotes epigenetic modification of exhausted T cells and improves anti-tumor immunityJCI InsightMarch 4, 2025Yanying Yang, Xiaoyan Li, Fangming Liu, Mingyue Ma, Ying Yang, Chengchao Ruan, Yan Lu, Xiaoyang Li, Xiangdong Wang, Yinghong Shi, et al.cells were isolated from the splenocytes using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, 480035). The sorted OT-1 CD8+ T cells were treated with L-2-HG (300 μM; Toronto Research Chemicals, H942596)

(24) NFAT single-deficient murine T cells reduce the risk of aGvHD while controlling cytomegalovirus infectioniScienceFebruary 21, 2025Nadine Hundhausen, Snigdha Majumder, Yin Xiao, Sigrun S. Haeusl, Helen Goehler, Rishav Seal, Cristina M. Chiarolla, Andreas Rosenwald, Matthias Eyrich, Luka Cicin-Sain, et al.instructions. CD4+ or CD8+ T cells were subsequently isolated using the negative Isolation kit (480033 or 480035, Biolegend) according to the manufacturer’s instructions.3 Allogenic hematopoietic stem cell transplantation CB6F1

(25) VSIG4+ tumor-associated macrophages mediate neutrophil infiltration and impair antigen-specific immunity in aggressive cancers through epigenetic regulation of SPP1J Exp Clin Cancer ResFebruary 7, 2025Zongfu Pan, Jinming Chen, Tong Xu, Anqi Cai, Bing Han, Ying Li, Ziwen Fang, Dingyi Yu, Shanshan Wang, Junyu Zhou, et al.room temperature for 5 min and centrifuged at 300 × g for 5 min. A CD8+ T cell sorting kit (Biolegend, 480035) was used for CD8+ T cell sorting under sterile conditions according to the manufacturer’s instructions.

(26) Aging impairs CD8 T cell responses in adoptive T-cell therapy against solid tumorsFrontiers in ImmunologyJanuary 24, 2025Gulfiya Kadyrzhanova, Miho Tamai, Shukla Sarkar, Rajkumar Singh Kalra, Hiroki Ishikawamin. CD8 T cells were negatively selected using a MojoSort Mouse CD8 T Cell Isolation Kit (Biolegend, 480035). CD8 T cells were then resuspended in complete RPMI-1640 media containing 2 mM L-glutamine, 1% HEPES

(27) Imaging of biphasic signalosomes constructed by checkpoint receptor 2B4 in conventional and chimeric antigen receptor-T cellsiScienceJanuary 17, 2025Ryohei Matsushima, Ei Wakamatsu, Hiroaki Machiyama, Wataru Nishi, Yosuke Yoshida, Tetsushi Nishikawa, Hiroko Toyota, Masae Furuhata, Hitoshi Nishijima, Arata Takeuchi, et al.assays ,, Mojo Mouse CD4 T Cells Isolation Kit,BioLegend,480033 Mojo Mouse CD8 T Cells Isolation Kit,BioLegend,480035 APC Annexin V Apoptosis Detection Kit with 7-AAD,BioLegend,640930 TNF-α ELISA Kit,BioLegend,430901 "CD19 MicroBeads, mouse

(28) CCR2 restricts IFN-γ production by hippocampal CD8 TRM cells that impair learning and memory during recovery from WNV encephalitisJournal of NeuroinflammationDecember 27, 2024Shenjian Ai, Artem Arutyunov, Joshua Liu, Jeremy D. Hill, Xiaoping Jiang, Robyn S. KleinPercoll gradient centrifugation using MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, cat. no. 480035). For Cre− and Cre+ mice, forebrain tissues were collected following cardiac perfusion. RNA was isolated

(29) Acetylcysteine synergizes PD-1 blockers against colorectal cancer progression by promoting TCF1+PD1+CD8+ T cell differentiationCell Communication and SignalingOctober 17, 2024Wenchang Zhou, Mengdi Qu, Ying Yue, Ziwen Zhong, Ke Nan, Xingfeng Sun, Qichao Wu, Jie Zhang, Wankun Chen, Changhong Miaospleens were surgically removed. Subsequently, we used a MojoSort™ mouse CD8 T Cell isolation kit (Cat # 480035, Biolegend), a negative screening method, to isolate CD8+ T cells from the spleen. Following spleen

(30) Activation of NOD1 on tumor-associated macrophages augments CD8(+) T cell-mediated antitumor immunity in hepatocellular carcinomaScience advancesOctober 4, 2024Zhang F, Jiang Q, Cai J, Meng F, Tang W, Liu Z, Lin X, Liu W, Zhou Y, Shen X, et al.isolated from the spleens of wild-type C57BL/6 mice using the CD8 T Cell Isolation Kit (BioLegend, #480035) after red blood cell lysis. For the proliferation assay, purified CD8+ T cells were labeled with 2

(31) RIG-I is an intracellular checkpoint that limits CD8+ T-cell antitumour immunityEMBO Molecular MedicineSeptember 25, 2024Xiaobing Duan, Jiali Hu, Yuncong Zhang, Xiaoguang Zhao, Mingqi Yang, Taoping Sun, Siya Liu, Xin Chen, Juan Feng, Wenting Li, et al.cells were further purified using the MojoSort™ Mouse CD8+ T-cell isolation kit (BioLegend, 480044 and 480035). After stimulation with PMA (100 ng/ml), ionomycin (2 μg/ml) and GolgiStop (1:1500 dilution) for 4

(32) Suppressive effect of the anesthetic propofol on the T cell function and T cell-dependent immune responsesScientific reportsAugust 20, 2024Yamamoto W, Hamada T, Suzuki J, Matsuoka Y, Omori-Miyake M, Kuwahara M, Matsumoto A, Nomura S, Konishi A, Yorozuya T, et al.CD4 T cells (CD44lowCD62Lhigh) were prepared using the MojoSort Mouse CD8 T cell isolation kit (cat#480035; BioLegend) and anti-CD44 mAb (cat#103004; BioLegend). Naïve CD4 T cells were prepared using a MojoSort Mouse CD4 T cell isolation kit

(33) 2-Bromo-1,4-Naphthalenedione promotes CD8(+) T cell expansion and limits Th1/Th17 to mitigate experimental autoimmune encephalomyelitisJournal of neuroinflammationJuly 27, 2024Yang C, Ma Y, Lu Q, Qu Y, Li Y, Cheng S, Xiao C, Chen J, Wang C, Wang F, et al.regions. Naive CD8+ T cells were isolated using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, 480035). The leukocytes and naive CD8+ T cells were then adjusted to a cell concentration of 1 × 106 cells/mL

(34) Copper(II)-Based Nano-Regulator Correlates Cuproptosis Burst and Sequential Immunogenic Cell Death for Synergistic Cancer ImmunotherapyBiomaterials ResearchJune 27, 2024Yingli Luo, Xianyu Luo, Yi Ru, Xinru Zhou, Didi Liu, Qian Huang, Maoyuan Linghu, Yuhang Wu, Zicheng Lv, Meimei Chen, et al.MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, #423801) were labeled with a CFSE Cell Division Tracker Kit (BioLegend, #480035) and subsequently added to the culture. After 48 h of coculture, the proliferation of the CFSE-labeled

(35) Chemoradiotherapy-induced ACKR2(+) tumor cells drive CD8(+) T cell senescence and cervical cancer recurrenceCell reports. MedicineMay 21, 2024Dai D, Pei Y, Zhu B, Wang D, Pei S, Huang H, Zhu Q, Deng X, Ye J, Xu J, et al.130-045-201 Human CD11b MicroBeads,Miltenyi Biotec,Cat# 130-049-601 Mouse CD8 MojoSort™ kit,Biolegend,Cat# 480035 SB-431542,MCE,Cat# HY-10431 LY294002,Selleck,Cat# S1105 MK-2206 2HCl,Selleck,Cat# S1078 Rapamycin (Sirolimus

(36) Induction of immortal-like and functional CAR T cells by defined factorsThe Journal of experimental medicineMay 6, 2024Wang L, Jin G, Zhou Q, Liu Y, Zhao X, Li Z, Yin N, Peng M.(both male and female mice were used). CD8+ T cells were purified using a negative selection kit (Cat# 480035; BioLegend), and the purified CD8+ T cells were activated with 1 μg/ml anti-CD3 (Cat# BP0001-1, RRID:

(37) Multimodal probing of T-cell recognition with hexapod heterostructuresNature MethodsMay 1, 2024Xiaodan Huang, Lingyuan Meng, Guoshuai Cao, Aleksander Prominski, Yifei Hu, Chuanwang Yang, Min Chen, Jiuyun Shi, Charles Gallagher, Thao Cao, et al.by negative selection with the MojoSort™ Mouse CD4+/CD8+ T-cell Isolation Kits (Biolegend, 480033, 480035). After three washes, cells were resuspended in the complete medium for use. NALM-6 cells were a generous

(38) Single-cell adhesive profiling in an optofluidic device elucidates CD8(+) T lymphocyte phenotypes in inflamed vasculature-like microenvironmentsCell Rep MethodsApril 22, 2024Camargo CP, Alapan Y, Muhuri AK, Lucas SN, Thomas SN.assays,Critical commercial assays,Critical commercial assays ,, MojoSort Mouse CD8a Selection Kit,Biolegend,Cat#480035 Zombie Red Fixable Viability Kit,Biolegend,Cat#423110 Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link,Abcam,Cat#ab269823 ,, Experimental models

(39) Sulforaphane activates CD8+ T cells antitumor response through IL-12RB2/MMP3/FasL-induced MDSCs apoptosis'Journal for ImmunoTherapy of CancerJanuary 31, 2024Jinyan Liu, Huanan Chen, Caijuan Guo, Jieyao Li, Miaomiao Li, Ming Zhao, Ziyi Fu, Zhen Zhang, Feng Li, Xuan Zhao, et al.levels. T-cell suppression assay CD8+ T cells were recovered from the spleens using magnetic sorting (Cat.480035, BioLegend, USA) and activated by the addition of CD3 (Cat.100238, BioLegend, USA, 10 ng/mL) and CD28

(40) Inhibiting PLA2G7 reverses the immunosuppressive function of intratumoral macrophages and augments immunotherapy response in hepatocellular carcinomaJournal for immunotherapy of cancerJanuary 25, 2024Zhang F, Liu W, Meng F, Jiang Q, Tang W, Liu Z, Lin X, Xue R, Zhang S, Dong L.the spleens of wild-type C57BL/6 mice was carried out using the CD8 T Cell Isolation Kit (BioLegend, #480035) in accordance with the manufacturer’s instructions. For preactivation, purified CD8 T cells were quantified

Write a Review

(1)

11/16/2020Great Murine CD8 Isolation Kit Meng SunThe isolation is 90% clean and CD8s are always enriched.
(1)

1/10/2020Good Isolation Kit to Recover Untouched Mouse CD8+ T CellsDr. Min Hee OhWe are very happy in the end using this kit since it's very cheap per isolation. Yield and purity is very acceptable especially with transgenic mouse T cells.
(1)

1/6/2020Good BioLegend Mouse CD8 T Cells Isolation Kit Wei XuYou can use this kit to get acceptable yield and purity to isolate CD8 cells from mouse with reasonable price.
(1)

6/18/2019Easy-to-Use Isolation Kit Meng SunThis kit is a great alternative to other costly brands. The purity is great, the yield is good, and changing to this brand has cut our lab's cost by a few thousands of dollar a year.

MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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