pRL-CMV Vector from Promega

Product Specs
ItempRL-CMV Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2261
Vector NamepRL-CMV
ResistanceAmpR
ReporterRenilla luciferase
Molecular Weight~66kDa
Selectable MarkerAmpicillin
PromoterCMV Enhancer and Immediate early promoter
Quantity20microg
HostMammalian

Specifications/Features

Vector Size:
4079 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(161) E2F activators signal and maintain centrosome amplification in breast cancer cellsMolecular and cellular biologyJuly 1, 2014Lee MY, Moreno CS, Saavedra HI.cell lines were cotransfected with either pGL3-Nek2, pGL3-Plk4 or mutant constructs along with pRLCMV (E2261; Promega) as an internal control using TransIT-2020 transfection reagent (MIR5400; Mirus, Madison, WI)

(162) Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in miceFree radical biology & medicineMarch 1, 2014Tomasi ML, Ryoo M, Yang H, Iglesias Ara A, Ko KS, Lu SC.respectively. The luciferase activity was normalized to Renilla luciferase activity (pRL-CMV, cat. # E2261, Promega, Madison, WI). Each experiment was done in triplicates. Western blot and Co-immunoprecipitation (Co-IP

(163) Luciferase-based reporter to monitor the transcriptional activity of the SIRT3 promoterMethods in enzymologyJanuary 1, 2014Satterstrom FK, Haigis MC.renilla control vector (used to control for cell number and transfection efficiency, Promega, cat. # E2261). The exact amounts of these two plasmids should be empirically determined for each assay in order to

(164) Synergistic Antiproliferative Effects of Combined γ -Tocotrienol and PPAR γ Antagonist Treatment Are Mediated through PPAR γ -Independent Mechanisms in Breast Cancer CellsPPAR researchJanuary 1, 2014Malaviya A, Sylvester PW.X3-TK-luc (Addgene plasmid no.1015) [31] and 3.2 ng of Renilla luciferase plasmid per well (Promega E2261) and then cotransfected with scrambled or PPARγ siRNAs using 0.8 μL of lipofectamine 2000 transfection

(165) Epigenetic regulation of Sox4 during palate developmentEpigenomicsAugust 20, 2013Ratnam S Seelan, Partha Mukhopadhyay, Dennis R Warner, Cynthia L Webb, Michele Pisano, Robert M Greeneplasmid containing the Sox4 promoter (Sox4/pGL3) and 50 ng/ml of a control vector (pRL-CMV; Cat. No. E2261; Promega) expressing Renilla luciferase were transfected into freshly isolated murine embryonic maxillary

(166) DNA methylation silences miR-132 in prostate cancerOncogeneJanuary 3, 2013A Formosa et al.nmoles of scrambled or premiR-132 sequences, and 10 ng of Renilla luciferase pRL-CMV vector (Promega E2261) were co-transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s suggestions.

(167) HIV Type 1 Viral Infectivity Factor and the RUNX Transcription Factors Interact with Core Binding Factor β on Genetically Distinct SurfacesAIDS Research and Human RetrovirusesDecember 1, 2012Judd F. Hultquist, Rebecca M. McDougle, Brett D. Anderson, Reuben S. Harris(E1751; Promega). The CMV-Renilla luciferase vector transfection control was obtained from Promega (E2261; phRL-CMV). Cell lines The CBFb-knockdown Human Embryonic Kidney 293T (HEK293T) cell line stably expressing

(168) Interferon-inducible protein Mx1 inhibits influenza virus by interfering with functional viral ribonucleoprotein complex assemblyJournal of virologyDecember 1, 2012Verhelst J, Parthoens E, Schepens B, Fiers W, Saelens X.polymerase II promoter sequence using the SnaBI and HpaI restriction sites. The pRLCMV plasmid (catalog no. E2261; Promega), which contains a Renilla luciferase gene under the control of a cytomegalovirus (CMV) promoter, was

(169) Silencing of miR-370 in Human Cholangiocarcinoma by Allelic Loss and Interleukin-6 Induced Maternal to Paternal Epigenotype SwitchPLoS ONEOctober 22, 2012Fangmei An, Sumitaka Yamanaka, Sarah Allen, Lewis R. Roberts, Gregory J. Gores, Timothy M. Pawlik, Qing Xie, Masaharu Ishida, Esteban Mezey, Anne C. Ferguson-Smith, Yuriko Mori, Florin M. Selaruor mutated pGL4 vector (firefly luciferase) and internal control pRL-CMV (Renilla luciferase, Cat# E2261; Promega, Madison, WI, US) vector. 48 hours after plasmid vector transfection, the luciferase reporter assay

(170) MicroRNA-mediated regulation of gene expression is affected by disease-associated SNPs within the 3′-UTR via altered RNA structureRNA BiologyJune 6, 2012Ulrike Haas, Georg Sczakiel, Sandra D. LaufermiR-155_SNP. For normalization a control-plasmid coding for Renilla luciferase (pRL-CMV, Promega, # E2261, 0.25 ng per well) was added. For MRAS reporter gene assays pmirGLO MRAS 3′-UTR constructs (either C

(171) The protective effect of tianeptine on Gp120-induced apoptosis in astroglial cells: role of GS and NOS, and NF-κB suppressionBritish journal of pharmacologyNovember 1, 2011Janda E, Visalli V, Colica C, Aprigliano S, Musolino V, Vadalà N, Muscoli C, Sacco I, Iannone M, Rotiroti D, et al.

(172) Transcriptional analysis of the titin cap geneMolecular genetics and genomics : MGGMarch 1, 2011Zhang S, Londhe P, Zhang M, Davie JK.luciferase reporter assays. The pGL3-Tcap constructs (0.2 μg, this lab) and pRL-CMV (0.01 μg, Promega/#E2261) were co-transfected into cells grown in 96 well plates using calcium phosphate. The positive control

(173) Tristetraprolin regulates the stability of HIF-1alpha mRNA during prolonged hypoxiaBiochemical and biophysical research communicationsJanuary 1, 2010Kim TW, Yim S, Choi BJ, Jang Y, Lee JJ, Sohn BH, Yoo HS, Yeom YI, Park KC.

(174) Estrogen regulation of thrombospondin-1 in human breast cancer cellsInternational journal of cancerSeptember 1, 2009Hyder SM, Liang Y, Wu J.luminometer (Berthold Detection Systems, GmbH). Renilla luciferase was expressed from pRL-CMV (Promega E2261). This plasmid was cotransfected with a consensus estrogen responsive element (ERE)-Luc at a ratio of

(175) Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cellsEndocrine-related cancerSeptember 1, 2009Hyder SM, Liang Y, Wu J, Welbern V.(Berthold Detection Systems, Huntsville, AL, USA). Renilla luciferase was expressed from pRL-CMV (Promega E2261). This plasmid was co-transfected with a consensus progesterone-responsive element (PRE)-Luc at a ratio

(176) Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryosBMC BiotechnologyDecember 1, 2008Francisca Alcaraz-Pérez, Victoriano Mulero, María L Cayuelaplasmids (Table 1). • Sea pansy (Renilla reniformis) reporter plasmids from Promega: pRL-CMV (Cat.# E2261), pRL-TK (Cat.# E2241) and/or pRL-SV40 (Cat.# E2231) (Table 1). • Adult zebrafish. • Egg water (60 mg/l

(177) Corneal Epithelial Cell Fate Is Maintained during Repair by Notch1 Signaling via the Regulation of Vitamin A MetabolismDevelopmental CellJune 29, 2007Sophie Vauclair, François Majo, André-Dante Durham, Norbert B. Ghyselinck, Yann Barrandon, Freddy Radtke

(178) Activation of retinoic acid receptor-dependent transcription by organochlorine pesticidesToxicology and Applied PharmacologyJune 2, 2004Géraldine Lemaire, Patrick Balaguer, Serge Michel, Roger Rahmani

(179) 1-deoxysphingolipids bind to COUP-TF to modulate lymphatic and cardiac cell developmentDevelopmental cellJanuary 1, 0001"Wang T, Wang Z, de Fabritus L, Tao J, Saied EM, Lee HJ, Ramazanov BR, Jackson B, Burkhardt D, Parker M, et al."Oligos.,This paper,N/A Recombinant DNA,, The Renilla luciferase (RL) plasmid pRL-CMV,Promega,Cat#E2261 PGL4.31[luc2p/GAL4UAS/Hygro] (luc2p),Promega,Cat#C935A Lenticrispr Version 2 vector,Addgene,Cat#52961 pHM1-Gal4(UAS),"Santori

(180) α-Catenin levels determine direction of YAP/TAZ response to autophagy perturbationNature communicationsJanuary 1, 0001"Pavel M, Park SJ, Frake RA, Son SM, Manni MM, Bento CF, Renna M, Ricketts T, Menzies FM, Tanasa R, et al."constructs were used in this study: 8XGTIIC-luciferase (no. 34615, Addgene)32, pCMV-Renilla Luciferase (E2261, Promega), empty mEmerald-C1 (no. 54734, Addgene), mEmerald-Alpha1-Catenin-C-18 (no. 53982, Addgene),

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pRL-CMV Vector

pRL-CMV Vector from Promega

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Specifications/Features

Promega

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pRL-CMV Vector from Promega

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