pRL-CMV Vector from Promega

Product Specs
ItempRL-CMV Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2261
HostMammalian
Vector NamepRL-CMV
ResistanceAmpR
ReporterRenilla luciferase
Quantity20microg
PromoterCMV Enhancer and Immediate early promoter
Molecular Weight~66kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4079 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(141) USP21 regulates Hippo pathway activity by mediating MARK protein turnoverOncotargetSeptember 8, 2017Nguyen HT, Kugler JM, Loya AC, Cohen SM.shRNAs 8xGTIIC-luciferase was a gift from Stefano Piccolo (Addgene plasmid #34615). The pRL-CMV (Renilla, #E2261) was purchased from Promega (Madison, WI, USA). HA/Flag-USP21 was a gift from Wade Harper (Addgene plasmid

(142) Direct Involvement of Androgen Receptor in Oxytocin Gene Expression: Possible Relevance for Mood DisordersNeuropsychopharmacologySeptember 1, 2017Dan Dai, Qiao-Chu Li, Qiong-Bin Zhu, Shao-Hua Hu, Rawien Balesar, Dick Swaab, Ai-Min Baoincubated with transfection reagent and plasmids PSV-AR and POXTA, together with the pRL-CMV vector (E2261, Promega, USA) as the internal control for 24 h, followed by testosterone treatment (10 − 5 nM) in DMEM,

(143) Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5Molecular cellJuly 20, 2017Flack JE, Mieszczanek J, Novcic N, Bienz M.TopFLASH,Addgene,"Veeman et al., 2003; Addgene#12456" Plasmid: pRL-CMV Renilla luciferase,Promega,Cat#E2261

(144) Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer CellsPPAR ResearchMarch 26, 2017Aneta A. Koronowicz, Paula Banks, Adam Master, Dominik Domagała, Ewelina Piasna-Słupecka, Mariola Drozdowska, Elżbieta Sikora, Piotr Laidlertransfected with 0.7 𝜇g X3 PPRE-TKluc plasmid (Cat. # 1015, Addgene, USA) and 0.7 𝜇g pRL control (Cat. # E2261, Promega, WI, USA) using Lipofectamine (Thermo Fisher Scientific, MA, USA) in OPTI-MEM medium (Thermo

(145) Propofol inhibits expression of angiotensin II receptor type 2 in dorsal root ganglion neuronsExperimental and Therapeutic MedicineMarch 1, 2017Bingbing Pan, Zhigang Cheng, Gaoyin Kong, Zongbin Song, Yunjiao Wang, Lai Wei, Dan Xiao, Yuan Zhao, Qulian Guoor 10 µM propofol with or without 50 µM LY294002, for 4 h. Co-transfection of the plasmid PRL-CMV (E2261; Promega Corporation) encoding Renilla reniformis luciferase (at one fifth molar ratio to test plasmids)

(146) miR-145-5p/Nurr1/TNF-α Signaling-Induced Microglia Activation Regulates Neuron Injury of Acute Cerebral Ischemic/Reperfusion in RatsFrontiers in molecular neuroscienceJanuary 1, 2017Xie X, Peng L, Zhu J, Zhou Y, Li L, Chen Y, Yu S, Zhao Y.normalized to those of cells by co-transfecting with the Renilla-luc-expressing vector pRL-CMV (Cat # E2261; Promega, Madison, WI, USA) at 5 ng/well. Gene Transfection and siRNA Interference Experiments in Vivo

(147) DUB3 Deubiquitylating Enzymes Regulate Hippo Pathway Activity by Regulating the Stability of ITCH, LATS and AMOT ProteinsPloS oneJanuary 1, 2017Nguyen HT, Kugler JM, Cohen SM.8xGTIIC-luciferase was a gift from Stefano Piccolo (Addgene plasmid # 34615). The pRL-CMV (Renilla, #E2261) was purchased from Promega (Madison, WI, USA). pBabe HA-LATS2 and HA-p130 AMOT expressing plasmids

(148) Pharmacologic modulation of RORγt translates to efficacy in preclinical and translational models of psoriasis and inflammatory arthritisScientific reportsDecember 1, 2016Xue X, Soroosh P, De Leon-Tabaldo A, Luna-Roman R, Sablad M, Rozenkrants N, Yu J, Castro G, Banie H, Fung-Leung WP, et al.RORγt (Genbank accession no. NP_001001523, aa 1–497), pFR-Luc reporter and pRL-CMV reporter (Promega #E2261) plus Carrier DNA (Clontech # 630440) using PEI solution (Sigma Aldrich cat# 40872-7) in a 96-well plate.

(149) MUC4 is negatively regulated through the Wnt/β-catenin pathway via the Notch effector Hath1 in colorectal cancerGenes & CancerAugust 24, 2016Priya Pai et al.plasmids # 12456 and # 12457, both were gifts from Randall Moon [49]). The pRenilla-CMV luciferase vector #E2261 (Promega; Madison, WI, USA) was used as an internal transfection control for all luciferase assay transfections,

(150) Role of Mitochondrial Dynamics in Neuronal Development: Mechanism for Wolfram SyndromePLoS biologyJuly 1, 2016Cagalinec M, Liiv M, Hodurova Z, Hickey MA, Vaarmann A, Mandel M, Zeb A, Choubey V, Kuum M, Safiulina D, et al.from Addgene (Cambridge, MA). The IP3R1 channel fragment (MmCD00312368) was from PlasmID, pRL-CMV (E2261) was from Promega Co., and mKate2-mito (FP187) was from Evrogen. XBP1ΔDBD-LUC was a kind gift from Dr.

(151) Mitochondrial biogenesis is required for axonal growthJournal of Cell ScienceJune 15, 2016Annika Vaarmann et al.(mitoDsRed, 632421) and eGFP (6085-1) were from Clontech. The Renilla luciferase reporter vector pRL-CMV (E2261) and firefly luciferase reporter pGL4.31[luc2P/GAL4UAS/Hygro] vector (C9351) were purchased from Promega.

(152) Cannabinoid WIN‑55,212‑2 mesylate inhibits ADAMTS‑4 activity in human osteoarthritic articular chondrocytes by inhibiting expression of syndecan‑1Molecular medicine reportsJune 1, 2016Kong Y, Wang W, Zhang C, Wu Y, Liu Y, Zhou X.Corporation, Madison, WI, USA), according to the manufacturer's protocol. Plasmid PRL-CMV (cat no. E2261; Promega Corporation) encoding Renilla reniformis luciferase was co-transfected with the reporter plasmid

(153) MYB-QKI rearrangements in angiocentric glioma drive tumorigenicity through a tripartite mechanismNature geneticsMarch 1, 2016Bandopadhayay P, Ramkissoon LA, Jain P, Bergthold G, Wala J, Zeid R, Schumacher SE, Urbanski L, O'Rourke R, Gibson WJ, et al.pGL4.10[luc2] vector (Promega) digested with XhoI and HindIII. The pRL Renilla Luciferase Reporter Vector (Promega E2261) served as an internal control in all assays. The mim-1 reporter construct and pRL renilla vector (ratio

(154) The canonical Wnt pathway regulates the metastasis-promoting mucin MUC4 in pancreatic ductal adenocarcinomaMolecular OncologyFebruary 1, 2016Priya Pai, Satyanarayana Rachagani, Imayavaramban Lakshmanan, Muzafar A. Macha, Yuri Sheinin, Lynette M. Smith, Moorthy P. Ponnusamy, Surinder K. Batraseven mutant TCF/LEF sites and was used as a negative control. The pRenilla-CMV luciferase vector #E2261 (Promega; Madison, WI, USA) was used as an internal transfection control in all luciferase assays, which

(155) Deubiquitylating enzyme USP9x regulates hippo pathway activity by controlling angiomotin protein turnoverCell discoveryJanuary 1, 2016Thanh Nguyen H, Andrejeva D, Gupta R, Choudhary C, Hong X, Eichhorn PJ, Loya AC, Cohen SM.(Cambridge, MA, USA plasmid # 34615). The pRL-CMV was purchased from Promega, Madison, WI, USA (Renilla, #E2261). The Myc-tagged ubiquitin expression vector was a gift from Hong Yi (IMCB). 480SBY vector was generated

(156) Enhancement of Radiation Sensitivity in Lung Cancer Cells by a Novel Small Molecule Inhibitor That Targets the β-Catenin/Tcf4 InteractionPloS oneJanuary 1, 2016Zhang Q, Gao M, Luo G, Han X, Bao W, Cheng Y, Tian W, Yan M, Yang G, An J.Tcf4 binding sites for β-catenin and the pRL-CMV Renilla-luciferase control reporter vector (Promega, E2261). Four hours after transfection, cells were treated with various concentrations of BC-23 or ICRT14.

(157) Functional Comparison of Mx1 from Two Different Mouse Species Reveals the Involvement of Loop L4 in the Antiviral Activity against Influenza A VirusesJournal of virologyNovember 1, 2015Verhelst J, Spitaels J, Nürnberger C, De Vlieger D, Ysenbaert T, Staeheli P, Fiers W, Saelens X.protein segment (3= 23 nucleotides [nt] and 5= 26 nt) of PHW198-NS (31). The pRL-CMV plasmid (catalog no. E2261; Promega), containing a Renilla luciferase gene under the control of a cytomegalovirus (CMV) promoter,

(158) MicroRNA-224 Induces G1/S Checkpoint Release in Liver CancerJournal of Clinical MedicineAugust 26, 2015Fangmei An, Alexandru V. Olaru, Esteban Mezey, Qing Xie, Ling Li, Klaus B. Piontek, Florin M. Selaruor mutated pGL4 vector (firefly luciferase) and internal control pRL-CMV (Renilla luciferase, Cat# E2261; Promega, Madison, WI, USA) vector. Next, 48 h after plasmid vector transfection, the luciferase reporter assay

(159) An HNF1α-regulated feedback circuit modulates hepatic fibrogenesis via the crosstalk between hepatocytes and hepatic stellate cellsCell researchAugust 1, 2015Qian H, Deng X, Huang ZW, Wei J, Ding CH, Feng RX, Zeng X, Chen YX, Ding J, Qiu L, et al.for 24 h were co-transfected with SHP-1 promoter vectors together with the control pRL-CMV vector (E2261, Promega). Luciferase activity was measured by Dual-Glo® Luciferase Assay System (E2920, Promega) 48

(160) Transforming growth factor beta receptor type III is a tumor promoter in mesenchymal-stem like triple negative breast cancerBreast Cancer ResearchAugust 1, 2014Bojana Jovanović, J Scott Beeler, Michael W Pickup, Anna Chytil, Agnieszka E Gorska, William J Ashby, Brian D Lehmann, Andries Zijlstra, Jennifer A Pietenpol, Harold L MosesUSA). Cells were transfected with 1.5 μg 3TP-Lux [22] or CAGA(9)-Luc [23]. pRL-CMV-renilla (Promega #E2261, Madison, WI, USA) was co-transfected and used as an internal control to correct for transfection efficiency.

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pRL-CMV Vector

pRL-CMV Vector from Promega

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Promega

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pRL-CMV Vector from Promega

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