pRL-CMV Vector from Promega

Product Specs
ItempRL-CMV Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2261
HostMammalian
Vector NamepRL-CMV
ResistanceAmpR
ReporterRenilla luciferase
Quantity20microg
PromoterCMV Enhancer and Immediate early promoter
Molecular Weight~66kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4079 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) Autophagy inhibition specifically promotes epithelial-mesenchymal transition and invasion in RAS-mutated cancer cellsAutophagyMay 1, 2019Wang Y, Xiong H, Liu D, Hill C, Ertay A, Li J, Zou Y, Miller P, White E, Downward J, et al.plates, followed by 24-h transfection with 250 ng of NFKB reporter and 10 ng of phRL-CMV (Promega, E2261), which constitutively expresses the Renilla luciferase reporter. One day before the measurement of

(122) Targeting FER Kinase Inhibits Melanoma Growth and MetastasisCancersMarch 24, 2019Ivanova IA, Arulanantham S, Barr K, Cepeda M, Parkins KM, Hamilton AM, Johnston D, Penuela S, Hess DA, Ronald JA, et al.upstream from sequences encoding firefly luciferase [50]. The Renilla luciferase-encoding plasmid pRL-CMV (E2261) was purchased from Promega (Madison, WI, USA). The small hairpin RNA (shRNA) transfer vectors containing

(123) Size-Dependent Effect of Silver Nanoparticles on the Tumor Necrosis Factor α-Induced DNA Damage ResponseInternational Journal of Molecular SciencesFebruary 27, 2019Alaa Fehaid, Akiyoshi Taniguchiof the luciferase encoding gene (luc+) in different types of mammalian cells. Also, pRL-CMV vector (E2261, Promega), which is a Renilla luciferase-encoding control plasmid, was used as an internal control for

(124) Development of a high efficient promoter finding method based on transient transfectionGene XFebruary 12, 2019Yao Lu, Qilong Li, Kexin Zheng, Chenghao Fu, Chunying Jiang, Dayu Zhou, Chao Xia, Shiliang MaThe mSEAP activity was assayed as previously. The pRL-CMV vector (Promega, Madison, WI, USA, cat. no. E2261) was included to normalize transfection efficiency in each transfection experiment. Cells were lysed

(125) Environmental Control of Astrocyte Pathogenic Activities in CNS InflammationCellJanuary 24, 2019Wheeler MA, Jaronen M, Covacu R, Zandee SEJ, Scalisi G, Rothhammer V, Tjon EC, Chao CC, Kenison JE, Blain M, et al.FLAG-HA-pcDNA3.1 (a gift from Adam Antebi, Addgene plasmid #52535) (Horn et al., 2014), and pRL-CMV-Renilla (#E2261, Promega). Luciferase activity was analyzed either 24 or 48 h post-transfection using the Dual Luciferase

(126) New Molecular Mechanism Underlying Myc-Mediated Cytochrome P450 2E1 Upregulation in Apoptosis and Energy Metabolism in the MyocardiumJournal of the American Heart AssociationJanuary 8, 2019Guan F, Yang X, Li J, Dong W, Zhang X, Liu N, Gao S, Wang J, Zhang L, Lu D.pRL‐CMV vector containing Renilla luciferase gene as the reporter was used as normalization (Promega, E2261). The constructs were all confirmed by DNA sequencing (see Table S1 for primer sequences and Table S2

(127) Transforming growth factor-β downregulates sGC subunit expression in pulmonary artery smooth muscle cells via MEK and ERK signalingAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologyJanuary 1, 2019Lili Du, Jesse D. RobertspCMV-RL, which encodes Renilla remiformis luciferase driven by a CMV promoter, was purchased from Promega (E2261). pCMV.myc·ERK2-MEK1 and pCMV.myc·ERK2-L4A-MEK1 (78) (plasmids 39194 and 39197, respectively) and pcDNA3.1Green

(128) Pathogenic FAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signallingWellcome open researchJanuary 1, 2019Wu KZL, Jones RA, Tachie-Menson T, Macartney TJ, Wood NT, Varghese J, Gourlay R, Soares RF, Smith JC, Sapkota GP.seeded in 12-well plates. 24 h later, 200 ng of SuperTOPFlash, 20 ng of Renilla luciferase (Promega, E2261), and 100 ng of GFP or PAWS1-GFP plasmids were co-transfected as described above. After 24 h, cells

(129) HDAC3 Regulates the Transition to the Homeostatic Myelinating Schwann Cell StateCell reportsDecember 4, 2018Rosenberg LH, Cattin AL, Fontana X, Harford-Wright E, Burden JJ, White IJ, Smith JG, Napoli I, Quereda V, Policarpi C, et al.enhancer construct: pGL3-P0-Int-Pro,"LeBlanc et al., 2006",N/A,N/A Renilla construct: pRL-CMV,Promega,Cat#E2261,Cat#E2261 ,,, Software and Algorithms,Software and Algorithms,Software and Algorithms,Software and

(130) The HNF1α-regulated lncRNA HNF1A-AS1 reverses the malignancy of hepatocellular carcinoma by enhancing the phosphatase activity of SHP-1Molecular CancerDecember 1, 2018Chen-Hong Ding et al.Lenti-HNF1α for 24 h were co-transfected with HNF1α-RE-LUC vectors together with the control pRL-SV40 vector (E2261, Promega). Luciferase activity was measured using the Dual-Glo Luciferase Assay System (E2920, Promega)

(131) The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulatingAjEHHADHduring aestivation in sea cucumberPeerJOctober 2, 2018Muyan Chen, Shanshan Wang, Xingke Li, Kenneth B. Storey, Xiumei ZhangpMIR-REPORT vectors were cotransfected with a control Renilla luciferase plasmid (pRL-CMV) (Cat No. E2261; Promega, Madison, WI, USA). The ratio of experimental plasmid to control plasmid was 5:1. Then vectors

(132) LUBAC and ABIN-1 Modulate TRAIL-Based NF-κB Induction in Human Embryonic Kidney 293 CellsBioResearch Open AccessJune 5, 2018Sebastian Dorn, Christian Schoergenhofer, Michael Krainer, Markus Müller, Bernd Jilmaoverexpression experiments 75 ng NFjB responsive reporter (#210978; Stratagene), 10 ng pRL-CMV vector (E2261; Promega) in combination with 10, 20, or 30 ng pCAGGS-E-hABIN-1 (LMBP 5126) or 10, 20, 30 ng pCAGGS-E-hABIN-1-MAD (LMBP

(133) Dietary and Microbial Oxazoles Induce Intestinal Inflammation by Modulating Aryl Hydrocarbon Receptor ResponsesCellMay 17, 2018Iyer SS, Gensollen T, Gandhi A, Oh SF, Neves JF, Collin F, Lavin R, Serra C, Glickman J, de Silva PSA, et al.DNA pGL4.43 Luc2pXRE,Promega,Cat# E4121 pGL4.75,Promega,Cat# E6931 Renilla luciferase,Promega,Cat# E2261 Software and Algorithms,Software and Algorithms,Software and Algorithms Graphpad Prism 7.0,GraphPad

(134) Activated ALK signals through the ERK–ETV5–RET pathway to drive neuroblastoma oncogenesisOncogeneMarch 15, 2018Lucille Lopez-Delisle et al.promoter or without promoter (400 ng); (3) the Renilla expression control vector (pRL-CMV, Promega, E2261) at a 1:400 dilution with respect to the luciferase vector. Luciferase assays were carried out 48 h

(135) Resolving systematic errors in widely used enhancer activity assays in human cellsNature MethodsFebruary 1, 2018Felix Muerdter et al.luciferase reporter plasmid and 2 µg of a Renilla luciferase control plasmid (pRL-CMV, Promega; cat# E2261). After 30 min, C16 and BX795 inhibitors were added to the cells at 1 µM concentration. qPCR to quantify

(136) Neofunctionalization of Duplicated P450 Genes Drives the Evolution of Insecticide Resistance in the Brown PlanthopperCurrent BiologyJanuary 22, 2018Christoph T. Zimmer, William T. Garrood, Kumar Saurabh Singh, Emma Randall, Bettina Lueke, Oliver Gutbrod, Svend Matthiesen, Maxie Kohler, Ralf Nauen, T.G. Emyr Davies, et al.reporter vector,Promega,Cat# E1751 pRL-CMV Renilla luciferase control reporter vector,Promega,Cat# E2261 ,, Software and Algorithms,Software and Algorithms,Software and Algorithms ,, Geneious v 9.1.8,Biomatters,https://www.geneious.com/download/ MEGA

(137) microRNA‑211 suppresses the growth and metastasis of cervical cancer by directly targeting ZEB1Molecular medicine reportsJanuary 1, 2018Chen G, Huang P, Xie J, Li R.pMIR‑REPORT miRNA Expression firefly Reporter vectors. pRL‑CMV was obtained from Promega Corporation (E2261). For the luciferase reporter assay, 293T cells were seeded in 24‑well plates at a density of 50‑60%

(138) qPCR based reporter assay on luciferase transcriptsProtocol ExchangeDecember 11, 2017Felix Muerdter et al.Mix \(2x) \(Promega, Cat No. A6001) pRL Renilla Luciferase Control Reporter Vector \(Promega, Cat No. E2261) Procedure **1. Transfection by electroporation with the MaxCyte STX transfection system** This protocol

(139) qPCR assay to measure ISG expression in human cellsProtocol ExchangeDecember 11, 2017Felix Muerdter et al.\(2x) \ (Promega, Cat No. A6001) pRL Renilla Luciferase Control Reporter Vector \(Promega, Cat No. E2261) pBluescript vector Procedure **1. Transfection by electroporation with the MaxCyte STX transfection

(140) Downregulation of miR-199a-5p promotes prostate adeno-carcinoma progression through loss of its inhibition of HIF-1αOncotargetOctober 13, 2017Zhong J, Huang R, Su Z, Zhang M, Xu M, Gong J, Chen N, Zeng H, Chen X, Zhou Q.reporter constructs using Lipofectamine 2000 (11668019; Invitrogen, Carlsbad, CA). The pRL-CMV plasmid (E2261; Promega, Madison, WI) containing the Renilla luciferase gene (0.02 µg) was cotransfected as an internal

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pRL-CMV Vector

pRL-CMV Vector from Promega

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Specifications/Features

Promega

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pRL-CMV Vector from Promega

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