pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Periodic expression of Per1 gene is restored in chipmunk liver during interbout arousal in mammalian hibernationSci RepFebruary 13, 2025Erina Nakamaru, Kota Seki, Yuiho Shirahata, Megumi Adachi, Nene Sakabe, Takuya Matsuo, Daisuke Tsukamoto, Nobuhiko TakamatsupCMPER1G/luc, and 2.5 ng of a Renilla luciferase internal control plasmid, pRL-SV40 (Promega, catalog no. E2231), using TransIT-LT1 reagent (Mirus, catalog no. MIR2300). Where indicated, the cells were co-transfected

(62) Chemogenomics for steroid hormone receptors (NR3)Commun ChemFebruary 3, 2025Espen Schallmayer, Laura Isigkeit, Lewis Elson, Susanne Müller, Stefan Knapp, Julian A. Marschner, Daniel Merkfor normalization of transfection efficiency and cell growth; Promega, Fitchburg, Wisconsin, USA; #E2231) and one pFA-CMV-hNR-LBD clone coding for the hinge region and ligand binding domain (LBD) of the human

(63) IFN-γ licenses normal and pathogenic ALPK1/TIFA pathway in human monocytesiScienceJanuary 17, 2025Amandine Martin, Solène Caron, Mélissa Marcotte, Pauline Bronnec, Etienne Garneret, Nora Martel, Georgina Maalouf, Pascal Sève, David Saadoun, Yvan Jamilloux, et al.paper,N/A pKLV-BFP-sgALPK1ex9,This paper,N/A pNF-kB-Luc,Stratagene,Cat#219077 pRL-TK,Promega,Cat#E2231 pKLV-BFP-sgTIFAex2#1,This paper,N/A pKLV-BFP-sgTIFAex2#1,This paper,N/A p20-ALPK1,This paper,N/A p20-TIFA,This

(64) The sequence–structure–function relationship of intrinsic ERα disorderNatureJanuary 8, 2025Zhanwen Du, Han Wang, Shuqi Luo, Zixi Yun, Chen Wu, Wangfei Yang, Matthias Buck, Wenwei Zheng, Alexandar L. Hansen, Hung-Ying Kao, et al.(Addgene, catalogue no. 46756) and 0.3 µg of pRL-Renilla-luciferase plasmid (Promega, catalogue no. E2231), using Lipofectamine 2000 (Invitrogen, catalogue no. 11668027). Twenty-four hours after transfection,

(65) Phosphorylation of the selective autophagy receptor TAX1BP1 by TBK1 and IKBKE/IKKi promotes ATG8-family protein-dependent clearance of MAVS aggregatesAutophagyJanuary 1, 2025White J, Choi YB, Zhang J, Vo MT, He C, Shaikh K, Harhaj EW.12260 VSV-G,[39],N/A pCL-Ampho,A gift from Shao-Cong Sun (MD Anderson Cancer Center),N/A IFN-β-Luc,[27],N/A pRL-TK,Promega,E2231 Plasmids used in the study.

(66) A Set of Proximal Regulatory Elements Contribute to the Transcriptional Activity of the Human Lipoprotein Lipase PromoterCurrent Issues in Molecular BiologyNovember 18, 2024Nasmah K. Bastaki, Taybha A. Albarjes, Afnan K. Mohamed, Noorhan H. Sabri, Suzanne A. Al-Bustancotransfected with 100 ng of each recombinant pGL4.10 [luc2] vector and 20 ng of the control Renilla vector (E2231; Promega, Madison, WI, USA) using LipofectamineTM Transfection Reagent (18324012; Invitrogen, Eugene,

(67) TSC/mTORC1 mediates mTORC2/AKT1 signaling in c-MYC–induced murine hepatocarcinogenesis via centromere protein MJournal of Clinical InvestigationNovember 15, 2024Yi Zhou, Shu Zhang, Guoteng Qiu, Xue Wang, Andrew Yonemura, Hongwei Xu, Guofei Cui, Shanshan Deng, Joanne Chun, Nianyong Chen, et al.plasmid (catalog E1751) and the pRL-CMV Renilla luciferase control reporter vector plasmid (catalog E2231) were purchased from Promega. To generate the pGL3-CENPM promoter plasmid, a 2000bp DNA fragment of

(68) Development of luciferase-based highly sensitive reporters that detect ER-associated protein biogenesis abnormalitiesiScienceNovember 15, 2024Hiroshi Kadokura, Nanshi Harada, Satoshi Yamaki, Naoya Hirai, Ryusuke Tsukuda, Kota Azuma, Yuta Amagai, Daisuke Nakamura, Kota Yanagitani, Hideki Taguchi, et al.DNA,Recombinant DNA ,, pcDNA3.1+,Invitrogen,Cat#V79020 pET15b,Novagen,Cat#69661 pRL-SV40,Promega,Cat#E2231 pFL-SV40-ER [pRL-SV40 ΔRLuc Calr (17 aa)-FLuc-KDEL w/o N197Q],Nakamura et al.35,N/A pHK960 [pRL-SV40

(69) Structural basis of the human transcriptional Mediator regulated by its dissociable kinase moduleMolecular CellOctober 17, 2024Ti-Chun Chao, Shin-Fu Chen, Hee Jong Kim, Hui-Chi Tang, Hsiang-Ching Tseng, An Xu, Leon Palao, Subash Khadka, Tao Li, Mo-Fan Huang, et al.563–700)(GGK/RRE),This paper,N/A Plasmid: pGEX6P-1-GST-VP16,This paper,N/A Plasmid: pRL-TK,Promega,Cat#E2231 Plasmid: p5xGal4-TATA-luc,Sun et al.61,"Addgene, Cat#46756" Plasmid: Gal4-VP16,Budzynski et al.62,"Addgene,

(70) TRAF7 determines circadian period through ubiquitination and degradation of DBPCommunications BiologyOctober 8, 2024Shusaku Masuda, Nobuhiro Kurabayashi, Rina Nunokawa, Yuta Otobe, Hiroko Kozuka-Hata, Masaaki Oyama, Yuri Shibata, Jun-ichiro Inoue, Michinori Koebis, Atsu Aiba, et al.3xD-box #1_Luc/pGL3N was constructed in the previous study5. pRL-SV40 was purchased from Promega (#E2231). Short-hairpin RNA (shRNA) were constructed as previously described30. Following sequences are designed

(71) EZH2 specifically regulates ISL1 during embryonic urinary tract formationScientific reportsOctober 2, 2024Mingardo E, Kalanithy JC, Dworschak G, Ishorst N, Yilmaz Ö, Lindenberg T, Hollstein R, Felger T, Angrand PO, Reutter H, et al.11668019) with 500 ng reporter and 50 ng pRL Renilla Luciferase Control Reporter vector (Promega Cat. No. E2231). Luciferase intensity was measured 24 h after transfection with Dual-Luciferase® Reporter Assay System

(72) Impaired endocytosis and accumulation in early endosomal compartments defines herpes simplex virus-mediated disruption of the nonclassical MHC class I-related molecule MR1The Journal of biological chemistryOctober 1, 2024Samer C, McWilliam HEG, McSharry BP, Burchfield JG, Stanton RJ, Rossjohn J, Villadangos JA, Abendroth A, Slobedman B.NucleoBond Xtra Midi kit (Macherey-Nagel, #740410) and transfected into T-REx-293 using FuGene HD (Promega, #E2231). After several days the infected cells were collected, and the pellet lysed by resuspending in equal

(73) TSC/mTORC1 mediates mTORC2/AKT1 signaling in c-MYC–induced murine hepatocarcinogenesis via centromere protein MJ Clin InvestSeptember 26, 2024Yi Zhou, Shu Zhang, Guoteng Qiu, Xue Wang, Andrew Yonemura, Hongwei Xu, Guofei Cui, Shanshan Deng, Joanne Chun, Nianyong Chen, et al.plasmid (catalog E1751) and the pRL-CMV Renilla luciferase control reporter vector plasmid (catalog E2231) were purchased from Promega. To generate the pGL3-CENPM promoter plasmid, a 2000bp DNA fragment of

(74) The Pentamer glycoprotein complex inhibits viral Immediate Early transcription during Human Cytomegalovirus infectionsProceedings of the National Academy of SciencesSeptember 24, 2024Michael S. Ohman, Emily R. Albright, Christopher B. Gelbmann, Robert F. Kalejtacotransfected with the indicated MIEP-driven Firefly luciferase reporter along with pRL-TK (Promega; E2231), as an internal Renilla luciferase control, and the designated EV, UL138 or Pentamer subunit construct.

(75) From Genome-wide Association Studies to Functional Variants: ARL14 Cis-regulatory Variants Are Associated With Severe MalariaThe Journal of Infectious DiseasesSeptember 23, 2024Mathieu Adjemout, Frederic Gallardo, Magali Torres, Alassane Thiam, Babacar Mbengue, Alioune Dieye, Sandrine Marquet, Pascal Rihetinstructions. For each assay, 106 cells were co-transfected with 200 ng of control vector pRL-SV40 (catalog # E2231, Promega) and 1 µg of vector tested. Empty pGL4.12-basic vector was used as a control. After transfection,

(76) Repression of SMAD3 by STAT3 and c-Ski induces conventional dendritic cell differentiationLife Science AllianceSeptember 1, 2024Jeong-Hwan Yoon, Eunjin Bae, Yasuo Nagafuchi, Katsuko Sudo, Jin Soo Han, Seok Hee Park, Susumu Nakae, Tadashi Yamashita, Ji Hyeon Ju, Isao Matsumoto, et al.or GM-CSF plus IL-4–induced BMDCs with a control TK-pRL Renilla luciferase control reporter plasmid (E2231; Promega). Five to seven days after transfection, BMDCs were lysed by Dual-Luciferase Reporter Assay

(77) AUTS2 disruption causes neuronal differentiation defects in human cerebral organoids through hyperactivation of the WNT/β-catenin pathwayScientific reportsAugust 22, 2024Geng Z, Tai YT, Wang Q, Gao Z.of AUTS2 on the WNT activity, M50 Super 8 × TOP-Flash and pRL-SV40 Renilla luciferase (Promega, Cat# E2231) were co-transfected into WT and AUTS2-/- HEK293T cells using Lipofectamine 2000. A mutant TOP-Flash

(78) Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptorsCell Chemical BiologyAugust 15, 2024Andrew N. Dates, Daniel T.D. Jones, Jeffrey S. Smith, Meredith A. Skiba, Maria F. Rich, Maggie M. Burruss, Andrew C. Kruse, Stephen C. BlacklowDNA pFuse-hIgG1-Fc2,InvivoGen,Cat# pfuse-hg1fc2 pcDNA3.1,ThermoFisher Scientific,Cat# V79020 pRL-TK,Promega,Cat# E2231 All plasmids encoding G Protein-Coupled Receptor constructs,This study,See Table S1 Luciferase reporter

(79) Loss of histone deubiquitinase Bap1 triggers anti-tumor immunityCell Oncol (Dordr)August 14, 2024Hong Chang, Mingxia Li, Linlin Zhang, Meng Li, Swee Hoe Ong, Zhiwei Zhang, Jie Zheng, Xiang Xu, Yu Zhang, Jing Wang, et al.Lipofectamine LTX with 0.5 mg of the vector constructed and 0.5 mg of the Renilla pRL-SV40 vector (Promega, E2231) as an internal control per well. After 36 h of transfection, relative luciferase units (RLUs) were

(80) The mevalonate pathway contributes to breast primary tumorigenesis and lung metastasisMolecular OncologyAugust 9, 2024Javier Conde, Isabel Fernández‐Pisonero, L. Francisco Lorenzo‐Martín, Rocío García‐Gómez, Berta Casar, Piero Crespo, Xosé R. BustelopRL‐SV40 vector (encoding the Renilla luciferase) was obtained from Promega (Madison, WI, USA; Cat. No. E2231). The pSynSRE‐T‐Luc vector (reporter plasmid encoding luciferase under the control of the Hmgcs1 promoter)

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

Citations (323)

Reviews

pRL-SV40 Vector from Promega

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